[Wed Aug 24 17:59:27 2016] >Input file: TM1Z98ATD.pdb >TM segments: A40-59_A116-140_A161-182_A197-214_A239-260 MAIDEN score = -54.5805 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I169A-I251A 1.25685539272092 E168A-I244A 1.22473150950152 L165A-I244A 1.1085279404989 V179A-I202A 0.855737994937773 L175A-I202A 0.811163683781021 F173A-V255A 0.737690948144749 L49A-C132A 0.712659865924541 L49A-Q126A 0.705170748098325 V179A-L197A 0.685912203966639 K161A-I244A 0.673380099174651 A182A-P201A 0.661598270523314 V176A-G252A 0.639436821132906 I169A-P249A 0.632031729186042 F45A-C127A 0.62733783010327 T48A-C127A 0.615998679178301 F51A-G133A 0.612715920531134 F45A-A130A 0.605695564044002 V179A-A198A 0.582989210806216 F180A-A257A 0.582249356006006 I42A-A125A 0.579017374502857 L49A-A130A 0.573644026590345 V174A-A256A 0.563564182068141 E168A-F245A 0.553804902725287 F51A-A130A 0.552783507461401 L165A-F245A 0.546999344985859 L52A-V134A 0.535633547879748 L175A-V206A 0.51863416312995 F45A-G129A 0.473646326281733 L49A-I131A 0.460899876089166 G171A-T213A 0.446043166928567 Y177A-A257A 0.444701495184187 T48A-G133A 0.431134348452289 T172A-G248A 0.426569481688004 F45A-L128A 0.423529730235897 Y53A-Q126A 0.416311987038996 L175A-V209A 0.414099519821969 F45A-Y122A 0.414086764708545 Y177A-A253A 0.408541906982622 L165A-V247A 0.406819304132309 V176A-A257A 0.403127667461898 A182A-P199A 0.386038005740333 A40A-A119A 0.377246974417864 L52A-C132A 0.376499000117043 F173A-I251A 0.372834397121872 T178A-I202A 0.368297247016911 I169A-G252A 0.356344718783354 Y177A-G203A 0.350233877125683 I169A-F250A 0.347325922313514 F180A-A256A 0.335245040574947 A164A-I244A 0.319717793028847 L49A-G129A 0.316970646995621 A41A-V121A 0.305816323854101 E168A-Q242A 0.304851667831449 L49A-V134A 0.302133044073299 A47A-Q126A 0.299170796071671 L52A-A57A 0.292490505316875 A182A-I202A 0.290803148051685 T213A-P249A 0.289792804643735 L50A-A130A 0.287924218045006 Y53A-L136A 0.285308540927447 S181A-A205A 0.27762670935088 F45A-Q126A 0.276797843174982 F173A-P249A 0.273409024272798 A205A-A212A 0.271782614970906 A182A-A205A 0.258288522893699 D240A-V247A 0.255672302137552 A167A-T213A 0.249027740927344 T178A-A205A 0.249004520286283 L49A-G133A 0.248608445507575 L50A-G133A 0.246204809548884 L175A-A253A 0.240617548412665 Y177A-P199A 0.240016160407103 A182A-L197A 0.233735823064806 F180A-H260A 0.228727990281887 I169A-A253A 0.228312432039112 G171A-F245A 0.224802308730309 L50A-G135A 0.219836568216989 T163A-T172A 0.217976302736711 A57A-V134A 0.21590088081804 G171A-F250A 0.215123996882635 V176A-M208A 0.214216103789869 I124A-C132A 0.213928739701725 V174A-V209A 0.206898155400742 S181A-Y259A 0.206408362092006 E168A-V247A 0.202799100158907 I169A-G248A 0.201396910368858 V56A-I131A 0.199074196643673 I42A-G129A 0.197358756599236 I46A-G129A 0.196989057635597 I42A-C127A 0.196875785681434 T172A-I214A 0.194745928479485 L165A-T213A 0.1916270872601 W243A-G248A 0.190944140076748 V206A-I214A 0.189910863280648 Q242A-P249A 0.189604784642085 F45A-I124A 0.18948145681169 F173A-A253A 0.188975761549377 I244A-P249A 0.188319041285465 G166A-I244A 0.187576381656585 T55A-H210A 0.184762768483208 A130A-K138A 0.184290730549614 V59A-I214A 0.183653409409999 T55A-G135A 0.181477504508645 K161A-W239A 0.180318712916744 G203A-L211A 0.178861421254902 A47A-Y122A 0.178455204691782 V59A-H210A 0.177987149338812 G162A-I244A 0.176499465709212 Y177A-M208A 0.17612162741821 D241A-V247A 0.17512644294451 L175A-P199A 0.173574928974625 F173A-F250A 0.172672879477984 Y53A-A130A 0.167806390517633 T163A-A212A 0.166789378717658 G171A-V255A 0.166587384974171 T172A-I251A 0.165301398731807 F173A-A254A 0.165213758204592 F51A-V137A 0.164948836874868 I54A-A130A 0.164578336664702 L50A-V134A 0.163283628610216 F180A-A254A 0.162671229574164 T58A-G133A 0.161818469763687 V176A-G203A 0.161656322873482 G203A-A212A 0.161636831452177 T213A-I244A 0.16144022796112 L165A-W243A 0.15812115167835 G162A-P249A 0.156251650059646 V56A-F140A 0.154623497041896 L52A-I131A 0.154605861937526 I54A-I131A 0.150931438456828 Y177A-F250A 0.150876294042137 L49A-L128A 0.150556570268994 V174A-G203A 0.15036776977635 T163A-D241A 0.149754675973953 I170A-A257A 0.149588313230644 A164A-I251A 0.147655835562951 L49A-Y122A 0.146312511608375 I170A-A254A 0.146169074422472 V56A-V137A 0.145930579974586 I169A-I244A 0.145510786342132 F51A-H210A 0.145324572160637 V174A-F207A 0.144450672949452 I54A-C132A 0.144447456571293 T172A-G203A 0.143079858720121 S181A-F204A 0.141239263837643 A182A-F204A 0.140399931252841 T48A-G135A 0.140098268814511 I170A-V255A 0.13983755828189 V174A-A205A 0.139302771066887 I170A-G252A 0.139280164019198 I46A-I131A 0.138942558094921 E44A-A125A 0.138682980148049 D240A-W246A 0.1383450095634 I54A-G133A 0.136583210829516 E44A-I131A 0.135667683282793 T213A-G248A 0.135143465814413 T178A-P199A 0.134615424713805 K161A-T213A 0.134413497511478 A205A-A256A 0.131886937692197 Y177A-A198A 0.130986998370274 K161A-I169A 0.128909219360176 L175A-V255A 0.128232401499169 V179A-P201A 0.127823245069264 T55A-I131A 0.12779861209465 G203A-H210A 0.12767004065655 L50A-G129A 0.127441250591755 L175A-A205A 0.126744250334696 T163A-I214A 0.126343803403767 T55A-I214A 0.125980685560723 G252A-H260A 0.125648068119878 S181A-P201A 0.124302424034236 V174A-H210A 0.123356473491767 V174A-I202A 0.122816311383219 V176A-A198A 0.122374011175951 A47A-V134A 0.122296325318009 I54A-K138A 0.121856798110964 V179A-F204A 0.119729972908273 T178A-A254A 0.11827889947711 L175A-L211A 0.116522276063834 A198A-A205A 0.116459160864357 T55A-C132A 0.116153863847845 A40A-G129A 0.115945230963185 P249A-Y259A 0.115713489047395 V59A-G133A 0.114373014480145 A164A-L211A 0.114258020244092 V176A-V247A 0.113955321515658 A41A-C132A 0.113628761847499 I169A-Y259A 0.113447083690567 A182A-G203A 0.112440434958573 F45A-C132A 0.112340564179447 T55A-V209A 0.111990170032979 E168A-F173A 0.111870704187588 F51A-G203A 0.110722194275397 V56A-G129A 0.110316334229772 A41A-A130A 0.110287578509574 K161A-W246A 0.110240619236368 T178A-G252A 0.110025155640991 T55A-L136A 0.109786672345981 L50A-L128A 0.10831276436583 T163A-V209A 0.107665442394428 T48A-V56A 0.107387296685905 F173A-V206A 0.106919281278958 K161A-E168A 0.106592806518298 G166A-A253A 0.10643112170045 E168A-W246A 0.105927153219075 V59A-T213A 0.104251209850815 V174A-T213A 0.104250856209382 S181A-A253A 0.103453000038245 A164A-G252A 0.102670612320566 A41A-Q126A 0.102616296633686 A43A-R118A 0.102198637104609 I42A-Q126A 0.102171733150998 T172A-A257A 0.102036640883313 M123A-V247A 0.101364830215292 S181A-A254A 0.100999300758012 F51A-F207A 0.0992852938141127 A47A-V121A 0.0991353691195043 T172A-Y177A 0.0988338019549815 E168A-A254A 0.0985186779748251 I46A-G135A 0.0984822783955736 V179A-A254A 0.0980894062255562 A164A-W246A 0.0971408829530165 A47A-G135A 0.0969250320973787 E168A-D241A 0.0968634355495782 F45A-R118A 0.0965140970048091 A125A-V134A 0.0963007549618542 S181A-A258A 0.0952744132130117 G166A-F250A 0.0945079296927211 G171A-A254A 0.094145376244522 L175A-I214A 0.0938017842234385 L52A-I124A 0.0938017842234385 L175A-A212A 0.0929339279532462 T55A-K138A 0.0928257184816963 A40A-L117A 0.0926835374284873 E44A-L128A 0.091986824927862 M123A-P249A 0.091907252061838 G171A-T178A 0.091601822819859 L175A-A198A 0.0914216809417345 T178A-A258A 0.0894126234071084 V174A-A257A 0.0892959668915664 T163A-G171A 0.0883923232735626 I169A-A212A 0.0883875284968934 E168A-F250A 0.0878083764543103 A47A-C132A 0.0873267529703778 T178A-V209A 0.0860216683849053 V174A-Y259A 0.0855084079097257 A47A-I124A 0.0854868171589356 A119A-A253A 0.0854099978101619 V174A-A258A 0.0847561963982404 E44A-L50A 0.0846590235646799 A205A-A253A 0.0844834478017615 A43A-L120A 0.0837587017225356 G166A-A212A 0.083419334791909 V56A-K138A 0.0832439524155562 L52A-G129A 0.0831052166598689 G171A-G203A 0.082162238278765 T172A-F204A 0.0820619639594327 L175A-A257A 0.0820152050806158 I170A-H210A 0.0819668573294445 Q126A-G135A 0.0809327804521134 L165A-G248A 0.0807230550422497 L175A-Y259A 0.0804118224027786 V59A-L136A 0.0795479545717424 E44A-G133A 0.0794208984131912 T172A-A258A 0.0788809399065933 A41A-Y122A 0.0786538369386296 A47A-C127A 0.078471566667335 A47A-M123A 0.0761942514884608 A43A-A130A 0.0757616169024486 F180A-L200A 0.0751715419632366 A41A-L117A 0.0750990126850941 F51A-Y122A 0.0744341087006717 I244A-G252A 0.0743865934283523 V206A-G252A 0.0720097504110653 A164A-P249A 0.0717629870052505 K161A-D241A 0.0714399472670151 E168A-L211A 0.0707419719212705 L175A-T213A 0.0700391967258872 I169A-A257A 0.0698563957073217 T58A-V134A 0.0681981527974434 A125A-C132A 0.0668501999464994 G162A-W243A 0.0667559245770351 T48A-L136A 0.0664102647071466 Q126A-C132A 0.0662916544350951 G166A-V209A 0.0660522699561965 V56A-C132A 0.0659608962152665 L52A-F140A 0.0659336032754494 K161A-Q242A 0.0649460955576 I244A-A254A 0.0627650383161376 G162A-G252A 0.0624034830575502 C127A-F245A 0.0623695535545927 A43A-L197A 0.0620336811503091 R118A-C127A 0.0613149377262099 A167A-I251A 0.0607114983820616 A164A-V209A 0.0594663962453916 A43A-F51A 0.0593475620604842 L175A-M208A 0.0590662729322755 A40A-C127A 0.0580167222060046 M123A-C132A 0.0574898692157682 V176A-P249A 0.0572672275383495 R118A-I124A 0.0569852177682382 E168A-G252A 0.0567853001103721 L165A-I214A 0.0567684294862696 G166A-A256A 0.0567247977897081 F173A-T213A 0.0566153187111258 V206A-P249A 0.0563206809639816 I170A-L211A 0.0560264074709835 L49A-G135A 0.0555555933153054 A43A-C127A 0.0552889634771576 V56A-V134A 0.0551514253181415 V174A-L211A 0.0547663361211068 C127A-W246A 0.0542375948651463 V56A-C127A 0.054188083171093 I214A-I244A 0.053973302227238 F180A-F250A 0.0539140592471807 I42A-L120A 0.0532879250466616 L50A-A125A 0.0532407272192694 G248A-A256A 0.0531956725136755 T163A-I251A 0.0531283162569436 A167A-M208A 0.0529563593531379 G248A-A257A 0.0529013441129758 A182A-A256A 0.0525938019890677 G162A-I251A 0.0519661660960882 A167A-F207A 0.0517691915567529 T172A-S181A 0.0504604485372053 L49A-K138A 0.0492533373102941 A182A-A257A 0.0484676623918446 A40A-L116A 0.0482196009017493 A182A-H260A 0.0479172767633269 L197A-I202A 0.0472461494610883 A57A-A130A 0.0472169353363761 A167A-A253A 0.0467622538700485 I170A-I214A 0.0466547747415076 F51A-M123A 0.0464383987811365 L175A-A254A 0.046259169445138 L52A-F207A 0.0461280744077461 Y177A-V206A 0.0453999617547125 V179A-V255A 0.0452224568238453 D240A-F245A 0.0451555934896115 L49A-M123A 0.0449954254512126 F173A-P201A 0.0439866483931728 S181A-L200A 0.0437612923289377 Y53A-L128A 0.0436356603272134 Q242A-F250A 0.042849510472152 L52A-A130A 0.0427118479564581 S181A-I202A 0.0422154597653059 I170A-H260A 0.0421068492457559 A40A-L120A 0.0420231268006015 I169A-H210A 0.0419275986110583 I170A-T178A 0.0418791999246588 M123A-I131A 0.0415535324010226 V209A-I214A 0.0410915592611909 S181A-G203A 0.0407699261885873 T55A-V134A 0.0407698297177168 V176A-F207A 0.0403340366535712 I170A-Y259A 0.0399895164116031 I42A-R118A 0.0397607348041058 K161A-G248A 0.0396767490305023 V209A-G248A 0.0396255564296162 V179A-A258A 0.0389257013400268 Y122A-A130A 0.0384195113071422 V59A-F140A 0.036891241830349 A164A-V247A 0.0364135729851269 I169A-V209A 0.0360554016585022 T172A-L211A 0.0359280783201446 F173A-F204A 0.0357132187813782 C132A-F140A 0.0354809934213174 F180A-G252A 0.0354039385435554 L52A-A125A 0.0351871708173217 A57A-C132A 0.0350871093632197 I202A-L211A 0.0349103898538301 A43A-M123A 0.0343995663658356 I42A-M123A 0.0342425025996995 G133A-F140A 0.0340498680081271 V176A-P201A 0.0340245127175418 L165A-A253A 0.0333863038655733 L197A-A205A 0.0331005226990775 I251A-H260A 0.0330045761507951 E44A-G129A 0.032990903582677 L52A-A139A 0.0329098358423495 F173A-A212A 0.0323236201198567 L52A-M123A 0.0322691085224087 I54A-G129A 0.0318570691981601 I46A-V121A 0.0314858130727691 F180A-P199A 0.0312955884040487 F45A-A119A 0.0311341575040927 F180A-G203A 0.031033930421807 V247A-A256A 0.0305626488233539 W246A-A254A 0.0292327369834959 A164A-A212A 0.0291939853528556 I170A-A253A 0.0291070918612935 I54A-G135A 0.0285919743173717 V176A-P199A 0.0279178352051019 W243A-I251A 0.0278877170743372 V59A-K138A 0.0276180762295938 E168A-V209A 0.0274075342636593 T55A-A139A 0.0270281550194267 L52A-H210A 0.0269748537563652 Y177A-G248A 0.0268429192966302 V174A-A212A 0.0268356077320657 K161A-V247A 0.0267889627479809 F45A-L52A 0.0263751466266893 E168A-V255A 0.0260322919224549 T163A-Q242A 0.0257853102856995 G162A-F245A 0.0254809272854981 Y122A-F250A 0.025376771261449 L52A-G135A 0.0252366444348091 A119A-F250A 0.0251871992358579 T172A-H210A 0.0248527846239455 T163A-G248A 0.0246553494610959 G171A-V179A 0.0228619612649834 G162A-G171A 0.0226906301507026 V176A-L197A 0.022566277203607 G171A-A256A 0.0224643904521553 V209A-G252A 0.0223030250987724 Y122A-G129A 0.0212755666159423 A167A-H210A 0.021145497792368 T178A-M208A 0.0209171497112268 F245A-A253A 0.0206776649308362 G129A-V137A 0.0201445732612791 E168A-V176A 0.0200574194856529 A164A-Q242A 0.0199578272580356 L49A-F140A 0.0197338116763923 G166A-T213A 0.0195638121998567 F45A-M123A 0.0193963565714057 Y53A-C132A 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