[Wed Aug 24 17:18:40 2016] >Input file: TM2J8DATD.pdb >TM segments: H3-25_M28-67_M116-133_M146-169_M196-225_M268-291_L31-54_L89-109_L113-128_L176-192_L216-242 MAIDEN score = -76.6640 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I50M-D218L 10 F35M-Q46M 5.32688813090618 F41L-C92L 5.03762977190917 C108L-I113L 4.81918380657106 I270M-A188L 2.32256330546137 C108L-Y115L 1.96954401917711 S205M-V276M 1.52422627124337 V32M-P49M 1.4368324195218 M158M-I163M 1.32039402549957 A221M-I117L 1.30148716641854 I224L-I229L 1.21809222710962 W130M-F150M 1.14222577885711 E104L-I117L 1.14094349209453 W66M-I117M 1.12671631628902 W66M-M122M 1.1214903054537 I223M-H230L 1.08647448409424 P34M-L47M 1.07927654497536 W66M-A118M 0.995563707617094 M218M-I117L 0.994863040178149 I50M-L219L 0.972487633418533 N28M-L52M 0.96425378594227 F34L-W100L 0.929160386592958 L52M-D218L 0.895890097527476 F10H-V291M 0.887033288948383 F35M-L47M 0.879466267042899 V31L-R103L 0.852738470063975 T5H-F10H 0.823124869151375 L48L-I89L 0.823027115887871 I270M-L187L 0.814423733766682 W271M-A188L 0.812079701510755 F208M-V276M 0.804198120228286 T38L-F97L 0.803010558356494 I50M-V220L 0.799737776180903 H190L-I229L 0.798832050557474 Y210M-F180L 0.797933585630132 C108L-G114L 0.784401314546694 F123L-V241L 0.779655809333782 N44M-I224L 0.777903016857947 T146M-A192L 0.761036251834057 F216M-H230L 0.734162835248336 L286M-V291M 0.7302485290098 T146M-L189L 0.720846713664075 F208M-L275M 0.703368553277902 V159M-L285M 0.703340604008239 W66M-F121M 0.699058217411615 H219M-L234L 0.698552173205043 F216M-L187L 0.69434492868479 F179L-L236L 0.69198935389871 F216M-L234L 0.691848617251168 F34L-L102L 0.681370171097321 L215M-A269M 0.677431840835053 F208M-A273M 0.677152121261802 L209M-A273M 0.675161940649802 W130M-A147M 0.673444928565147 A273M-L185L 0.666601851629199 F97L-I125L 0.665626299898081 I206M-V276M 0.664675056601203 M65M-A118M 0.659564660194078 F201M-I282M 0.659144190648184 F10H-V290M 0.65450604310473 L224M-H230L 0.651189462050198 L55M-W129M 0.65073673494005 A213M-F180L 0.649333075502278 G35L-W100L 0.646796218071527 A101L-F121L 0.645632445136822 T5H-V290M 0.64411426967939 A207M-V276M 0.637402307745198 V276M-A184L 0.633366579837012 F34L-R103L 0.630819123185596 F41L-A93L 0.629380488895266 W66M-F120M 0.616656753374593 A217M-L238L 0.614725321909212 F61M-A125M 0.609210687821831 A13H-L286M 0.608335075727705 F201M-G283M 0.607282432170534 G56M-W129M 0.601061805908051 L160M-P165M 0.598554115807093 F208M-M272M 0.596097530349947 H202M-T279M 0.586233610497969 W157M-F162M 0.580974099423073 F34L-S99L 0.57939491367202 S62M-F121M 0.577598240442776 L209M-V276M 0.575301501107767 A13H-V291M 0.574604235424993 F216L-Y222L 0.574096183458003 L156M-L278M 0.574093030399996 S212M-L187L 0.572928902439361 L58M-A125M 0.571532295651395 V98L-A122L 0.569817136633915 F123L-F242L 0.569796615384528 L224M-H116L 0.56961776605829 L58M-S128M 0.56827714136794 L102L-P118L 0.562674946961555 S30M-L52M 0.558026798547413 F67M-A118M 0.557367462168979 L214M-S237L 0.553360333855848 F216L-S223L 0.54613948547105 A176L-F242L 0.544521186680455 F216M-A269M 0.542353958849092 S36M-L47M 0.540108567525909 I224L-R231L 0.538619606845359 A120L-L238L 0.537072371537723 L209M-F181L 0.533701748716873 M65M-F121M 0.532304359440559 A217M-L234L 0.528345505682159 V32M-L47M 0.523027942686221 V159M-I284M 0.521936520663992 F39L-A96L 0.520649686834636 A127L-F242L 0.519473406234918 I50M-Y222L 0.517847061521888 A149M-I270M 0.516847017364688 H219M-H230L 0.506500297419171 A101L-F119L 0.503538319853858 Y51M-V220L 0.495427728342027 I17H-F201M 0.493692802609832 F40L-A96L 0.49350773136983 R29M-G53M 0.491924292874078 F216M-A221M 0.49022452790464 W130M-L151M 0.489695172544985 A124L-V241L 0.487620377865468 Q46M-Y222L 0.480032851639505 L160M-I284M 0.478965874436835 N183L-G233L 0.478344477711508 F180L-L236L 0.477922836315905 I270M-L189L 0.477582874409725 T38L-W100L 0.473016345111656 T222M-H116L 0.469014188423249 Y51M-R217L 0.468929291973768 L52M-V57M 0.463431521419548 V32M-Q46M 0.463270043772864 F42M-R231L 0.459687955651576 F123L-L238L 0.459617921459718 A176L-S239L 0.459398231558671 S152M-L275M 0.458324365936277 I17H-P200M 0.457451929361054 G31M-I50M 0.452690929615574 A221M-H230L 0.450685940719245 W66M-S119M 0.448179520426467 T38L-E104L 0.447187407227598 R164M-L286M 0.44391986400087 M218M-A120L 0.442192341930769 A153M-V274M 0.441016730758761 R132M-L219L 0.439227060404711 V274M-A184L 0.437050498659422 N183L-L236L 0.4367670535886 N183L-H230L 0.435433333489311 S205M-L278M 0.432355460649366 L55M-S128M 0.429961120285524 L38M-A45M 0.429189812019187 L60M-A125M 0.428274058683094 I163M-L285M 0.426532246568989 T133M-L219L 0.4240612766827 L47M-R217L 0.422948063911924 L209M-F180L 0.421173588963752 F33L-R103L 0.419880132197501 L224M-R231L 0.417946298441387 P200M-G283M 0.414827265098918 A225M-I113L 0.410351443730052 T146M-A188L 0.407373012105175 A13H-V290M 0.407349432255888 G35L-S99L 0.405670657110613 A213M-A184L 0.404950835683783 I49L-I89L 0.401105563969451 W130M-M158M 0.40053812011885 A149M-L189L 0.39984987055714 L209M-A184L 0.398106868011617 A221M-A120L 0.397735873984652 L47M-S223L 0.394841015423483 T38L-A96L 0.392595561992799 G43M-I229L 0.39026524650403 F179L-S239L 0.388115239168207 R217L-I224L 0.387511766558596 A217M-F121L 0.385749434886872 A37L-A96L 0.385713077623745 Y51M-G221L 0.382733665884786 F201M-I284M 0.382446942930537 A149M-A192L 0.381483131890148 I270M-A192L 0.381134870566929 M65M-I117M 0.381026855549184 A101L-A122L 0.37769870508825 A37L-A93L 0.37605922236573 A213M-A188L 0.375864246542454 V98L-R103L 0.372951667006631 H219M-C108L 0.372550024516531 T222M-E104L 0.372315645456767 M218M-L234L 0.371872671663273 F42M-I224L 0.371736456983101 L12H-V290M 0.359969562949965 T182L-L236L 0.359192419833926 L209M-M272M 0.357064859051098 A153M-L278M 0.356575152808559 A213M-A176L 0.354725787554438 I50M-G221L 0.350739751110677 G63M-V126M 0.350553091459973 A221M-R231L 0.350356988306433 M168M-G288M 0.347026791804871 L196M-H202M 0.34467319253643 R29M-Y51M 0.344489992104473 A37L-A101L 0.34168394883451 S205M-L275M 0.341320839843934 L39M-Q46M 0.340892659936385 G45L-A93L 0.339395835416836 L215M-M272M 0.339361792871907 M158M-V274M 0.336433635386711 G40M-Q46M 0.334757361066201 E106L-G114L 0.334278933139094 G63M-V124M 0.333393227788516 L187L-G233L 0.33157148839614 A186L-G233L 0.330227965062049 I177L-A240L 0.330070277502701 R164M-T289M 0.329149793224802 S212M-H190L 0.326818182022974 S178L-A240L 0.326163919760566 A225M-I117L 0.325591040485916 N28M-R217L 0.325550564573112 S212M-A269M 0.323785510812309 H116L-H230L 0.323110186604094 A186L-G228L 0.323095865243966 A127L-S239L 0.321672564917913 G114L-A120L 0.318956454988322 W155M-L278M 0.318573872658795 H190L-S223L 0.318471346306439 G63M-F121M 0.318007107338059 A118M-G161M 0.317966020042098 G211M-M272M 0.316159493346247 G3H-G169M 0.312973799151464 A225M-A120L 0.312060498931982 A42L-A93L 0.311672511225605 S128M-A147M 0.309800228263461 L58M-V124M 0.309775917733326 S212M-A240L 0.30960795370421 Y51M-L219L 0.307895653179343 H219M-G228L 0.307527121960331 S59M-V126M 0.307079525276433 V36L-C92L 0.306546100017389 A273M-G280M 0.306183541236124 N28M-I50M 0.304433325317914 A13H-G288M 0.302947891833671 S205M-A184L 0.302325088356695 G220M-I229L 0.302191172170579 Y51M-G56M 0.301863555653676 N28M-R132M 0.301826003662114 A213M-L236L 0.300067865464736 V105L-P118L 0.299470746724952 V32M-I50M 0.298656876712347 S54M-G131M 0.295412585837212 S212M-G191L 0.29421523812764 A213M-L238L 0.293812999243422 V98L-I125L 0.292319697701999 H219M-M272M 0.29221996902959 L224M-G114L 0.29162057851982 L167M-T289M 0.291082648699387 M272M-H190L 0.290646346952418 G43M-L227L 0.2904465160045 L204M-V276M 0.289930484226999 S19H-P200M 0.289293659891677 V124M-W129M 0.288392616029485 A186L-I229L 0.288157809894492 L44L-C92L 0.287738466687211 G40M-S223L 0.287464004820795 R217L-Y222L 0.286006663979113 W157M-T279M 0.284886499462035 G161M-G169M 0.283815875493644 A120L-G233L 0.283258181870761 F216L-G221L 0.281053645286029 N199M-G288M 0.280795676990184 W157M-P165M 0.280479454967975 A120L-L234L 0.280192059467339 V4H-D11H 0.28008564950091 A217M-L235L 0.27890289947138 N44M-G228L 0.278691809258911 G203M-G281M 0.278293390340012 L151M-G281M 0.277908433933034 A217M-A269M 0.277398786933469 R109L-G114L 0.276960488941983 T277M-T182L 0.274943077453698 G31M-Y222L 0.274563274491131 W21H-P200M 0.274448905624554 T94L-I125L 0.272984759253447 G53M-D218L 0.272132331366061 A217M-A184L 0.272116172678635 S62M-M122M 0.272048099676222 R231L-L236L 0.271063122515422 V31L-C108L 0.270812160117651 T38L-G95L 0.270639139827856 N44M-G221L 0.270595544080846 A120L-A127L 0.2703440301978 S36M-G48M 0.268604258884562 H219M-Y115L 0.268241810858224 F197M-G203M 0.267146603455356 W66M-A125M 0.267096452218619 H219M-G114L 0.266831679280367 S205M-T277M 0.266205004836862 L160M-L285M 0.266160977347377 A176L-V241L 0.265783057346631 F216M-N183L 0.265317390204691 G56M-D218L 0.264507355523522 A186L-R231L 0.264257963715584 N199M-G281M 0.264185433014256 G32L-R103L 0.263116368444186 G63M-M122M 0.262583668159661 L215M-G191L 0.262202304850395 W148M-V274M 0.261474668914518 Y198M-G203M 0.26088056285124 W148M-G191L 0.260673478868098 S178L-L236L 0.260270722849393 S14H-G283M 0.259061712963394 A127L-V241L 0.258493772763424 H116L-L235L 0.257664617443516 V57M-D218L 0.256525211754415 H116L-L238L 0.256501052313 L60M-S128M 0.252362861792641 G220M-A186L 0.249220047078471 A217M-A122L 0.247225613187927 Y198M-S287M 0.246941376417909 H202M-L285M 0.246803902714781 W155M-G283M 0.245890913796525 R29M-Q46M 0.244502136586818 A225M-R109L 0.243721783616457 M218M-R231L 0.242949147580336 N9H-G288M 0.24205401554773 Y115L-A122L 0.240860540105988 W148M-H190L 0.238048619513102 S62M-V124M 0.237988590283527 T222M-I117L 0.237937514182121 A213M-A221M 0.235834336926318 R164M-I284M 0.23472120464076 H202M-L275M 0.234570134664748 L47M-G221L 0.233689074455398 A217M-F180L 0.232947932564612 W148M-T277M 0.232751019911541 L160M-T277M 0.232628577798545 V159M-P165M 0.232486555974658 F180L-S237L 0.232060279426126 P200M-L286M 0.231613802844781 T5H-T289M 0.231537911572378 I270M-H190L 0.230969522439652 W157M-G281M 0.230108233990516 H116L-G233L 0.229954696425901 L196M-G288M 0.229895782306333 F216M-S237L 0.229801052008082 A213M-F123L 0.229800401629374 G45L-I89L 0.226275606501144 S205M-S287M 0.225604063888863 G48M-D218L 0.224908918362233 M158M-R164M 0.224779241380452 L204M-T279M 0.223756610441058 G56M-W127M 0.223519118907141 R29M-Y222L 0.223311814359301 E104L-Y115L 0.223308435078765 F179L-S237L 0.223298619918115 N28M-G221L 0.223148199276884 L215M-H230L 0.221530005552558 G211M-V274M 0.219693164454545 H190L-L227L 0.219291780775109 V36L-G95L 0.219179220146329 L204M-G281M 0.218954831439949 F150M-A184L 0.218733327890344 G3H-G8H 0.218460337789141 Q46M-G228L 0.218070673307702 Y18H-A25H 0.216972173142625 G35L-V105L 0.216122447785078 L12H-P200M 0.215333964988472 A43L-G95L 0.214075167526047 A269M-H190L 0.213897431878864 V4H-M168M 0.213578327670676 S205M-A213M 0.21344160577133 V32M-D218L 0.211733049125696 V98L-F123L 0.211346029691169 G56M-V124M 0.209774729238542 S212M-G220M 0.209681825656018 S119M-P165M 0.208948945862974 M158M-V276M 0.208833833874237 L156M-V276M 0.208766770157447 W66M-V124M 0.208631024681882 T38L-T90L 0.207361229834154 L58M-G131M 0.207295208838101 V276M-F180L 0.206715673024042 G40M-T226L 0.206261905358893 I282M-V290M 0.205223319941364 G220M-L232L 0.204268219436383 D11H-A16H 0.203526123061462 N28M-G48M 0.202591969832049 W268M-G191L 0.202451736656333 M218M-N183L 0.202158901365221 W130M-W148M 0.201174975281771 T146M-L187L 0.200756706395973 V4H-F10H 0.200748526378188 S36M-Y222L 0.200418528262715 G53M-W129M 0.200369979079106 I224L-G233L 0.20001803901416 A16H-S287M 0.199510992782514 T182L-H190L 0.19942221356759 N44M-R231L 0.199267537408807 N28M-D218L 0.198652622975189 M158M-L286M 0.198363400892689 A221M-E104L 0.197775889555207 S152M-S212M 0.197476195784093 H219M-T226L 0.197474745252729 W271M-T279M 0.197388550566338 S54M-A125M 0.196150401715211 G43M-R231L 0.195529640436559 N199M-L286M 0.195491245575442 Y198M-G280M 0.195397435708674 I154M-G281M 0.195312619582837 N183L-R231L 0.195167221481553 L156M-I282M 0.194027434000795 F216M-A240L 0.193917152048751 R29M-V220L 0.193871889197099 A217M-A225M 0.193029048607453 F7H-V290M 0.192962181116385 N183L-G191L 0.192593905275639 L12H-G283M 0.192177849763109 G53M-S128M 0.191884215655159 V126M-I154M 0.19116822936788 I22H-T279M 0.190769847941628 F197M-H202M 0.189777635064969 W21H-T279M 0.189575825944476 A43L-T90L 0.188900620392285 I154M-G280M 0.18854857069844 W157M-I282M 0.188392790779248 L39M-A45M 0.188358071895743 G33M-P49M 0.187646245566651 I206M-G211M 0.187347263936727 G40M-L227L 0.186848434489093 G225L-G233L 0.18630715420758 G33M-N44M 0.186125857456344 M158M-S287M 0.186029497830535 A45M-T226L 0.185541526766635 A43L-T94L 0.185497028811549 S30M-G48M 0.18531145600684 F20H-G283M 0.185280969044608 L160M-G281M 0.184335900804879 P200M-T279M 0.183696821140482 A186L-L232L 0.183482208942433 I206M-G283M 0.183481364700065 T133M-A147M 0.183183153429258 L156M-G281M 0.183098456185258 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