[Wed Aug 24 17:36:00 2016] >Input file: TM2NS1ATD.pdb >TM segments: A18-35_A41-59_A105-120_A123-141_A163-181_A198-213_A234-252_A255-273_A284-303_A308-328 MAIDEN score = -74.9039 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- Y32A-C312A 1.9107410776094 M23A-L208A 1.63355424350359 F52A-C109A 1.63228831252893 M23A-I207A 1.54042802275803 I242A-A265A 1.47166466645482 M23A-I28A 1.47031727784212 A240A-V289A 1.30441556226057 L136A-I141A 1.22067878032155 T165A-C326A 1.1433406324754 A241A-V289A 1.09903443874161 F31A-V201A 1.07848342315805 W250A-P256A 1.0715110524576 W245A-L293A 1.03712815463984 V272A-I287A 1.00580338289615 V20A-W212A 0.978458510077501 F107A-V167A 0.974960137614623 L30A-M200A 0.9589798872921 I169A-H318A 0.953362208980352 V20A-F107A 0.923896098145403 A118A-D309A 0.885425553974198 I246A-P256A 0.883909257215171 I242A-A261A 0.882861632830302 E249A-L296A 0.86774514454834 I169A-I323A 0.860152202446358 F52A-V112A 0.848061266321535 T111A-V167A 0.833660417088723 F31A-T203A 0.832962330933802 F213A-T234A 0.823969911597698 W297A-L302A 0.809629880731612 I28A-L208A 0.808404361972848 W245A-L296A 0.804757694438035 T238A-A265A 0.798424345574254 C56A-G105A 0.772506747702197 I25A-T49A 0.745538202705666 T238A-I266A 0.741680622630468 I28A-L114A 0.740055288010592 Q47A-V116A 0.734382430127571 L30A-V201A 0.732895427345984 T51A-C109A 0.723805463386826 A120A-P311A 0.719787195054748 F315A-V320A 0.714465401988487 L176A-F315A 0.711386577378295 V270A-C321A 0.705098670036659 T205A-I266A 0.70362078860849 L21A-C109A 0.695944044878768 I173A-G319A 0.695719229644072 I287A-G325A 0.688540255666556 V236A-L285A 0.678217076771198 Y209A-L269A 0.676509991464262 D313A-H318A 0.676134871498457 V236A-G288A 0.675695621233992 I242A-S263A 0.674300033271042 T24A-F52A 0.674174052023155 W245A-A261A 0.671334533709639 F22A-I207A 0.667682045868673 T51A-V132A 0.66323674908424 I173A-F315A 0.65943882570266 Q47A-V128A 0.658437198183636 A108A-Y140A 0.656131329743192 A120A-D310A 0.651921929292051 Q47A-L129A 0.647095988817991 V270A-A291A 0.646937838983588 G268A-V289A 0.645353561250102 M44A-A120A 0.642569914499943 Y209A-I266A 0.631828124867729 W59A-A108A 0.629274232065756 F31A-M200A 0.628863979115788 L119A-P311A 0.626774558668708 A294A-V317A 0.618869253718653 T24A-I110A 0.613849506295971 F52A-G113A 0.612419903840008 W245A-V289A 0.609642830027894 W245A-G292A 0.609176849061354 G244A-V290A 0.594037021252174 G175A-F315A 0.588705948374222 F31A-F202A 0.586922074615088 P26A-G204A 0.586587748650394 G34A-V41A 0.576898939665576 I246A-K255A 0.576725949686123 V270A-G325A 0.57508112876253 I28A-G113A 0.569812733927879 G33A-V201A 0.568000781668843 L35A-L198A 0.56432936676701 A108A-V167A 0.562227947410251 T51A-V112A 0.560540515331864 Y140A-N170A 0.557565505433194 W212A-L269A 0.555715775155491 A171A-V314A 0.554240093812407 I115A-V314A 0.553835575729948 L114A-V314A 0.545233978103253 Y180A-D309A 0.544745977552032 W245A-G264A 0.543794857710122 W297A-K303A 0.538739516628512 F202A-L258A 0.531672738001249 T24A-V48A 0.526194773186623 I123A-V128A 0.525288949866756 L19A-F213A 0.525140235417468 A118A-F315A 0.522188824140315 C262A-C312A 0.520801962169696 F22A-L208A 0.519145462043347 F202A-L259A 0.517957182779396 K303A-V308A 0.512563663157656 T111A-H168A 0.508034794129778 L35A-V201A 0.507722866813229 L119A-D310A 0.506294473969204 T234A-A284A 0.505190502768737 T205A-A265A 0.498074991021757 I115A-A171A 0.496849049368855 L35A-C312A 0.496267397912434 V166A-A171A 0.492172506565836 A241A-L293A 0.489628270219092 Y140A-V166A 0.483961865516083 L21A-I110A 0.47935003602666 M23A-W212A 0.477581833326463 T238A-A267A 0.47750054471003 L42A-Q47A 0.475338424350353 S263A-V317A 0.474151405275729 A294A-V324A 0.473675559923837 I266A-V317A 0.466273961677379 A241A-V290A 0.46424260108656 V201A-C262A 0.463986907128241 A267A-H318A 0.463359461767034 M23A-C109A 0.462871687563099 L35A-P311A 0.458901065694254 L19A-I210A 0.457762017649097 L176A-D310A 0.457317157749097 V314A-G319A 0.454418963805399 F247A-P256A 0.453184252893595 A291A-V320A 0.452477291303079 I242A-G260A 0.452379632922601 G27A-T203A 0.443405669457885 Y209A-A265A 0.440422306366268 V55A-C109A 0.43522800316955 L30A-T203A 0.427207884736105 P26A-I207A 0.426801155065584 V55A-A108A 0.425888108923747 P256A-A261A 0.425122157184306 G298A-F315A 0.423356545791799 T51A-L129A 0.418591788373755 V116A-V128A 0.417704796913173 G117A-P311A 0.416249891454671 L176A-V308A 0.414697667414534 V55A-V133A 0.414513782870325 V272A-G288A 0.414381147561572 V55A-V112A 0.413975049114153 I286A-M328A 0.413921205374449 W135A-Y140A 0.413509535830999 V290A-V324A 0.41302297455833 A237A-L269A 0.411706321933012 V55A-V132A 0.411011372069384 T234A-V272A 0.410981904755168 T238A-G264A 0.408087505722406 Y32A-V201A 0.40768866117363 F52A-I110A 0.406476977674274 G271A-I323A 0.405397993531275 M23A-I210A 0.403914696087195 V201A-L259A 0.403758322543595 V317A-G322A 0.396344677778352 I242A-C262A 0.393052681292557 A237A-V289A 0.391726598418976 I169A-F315A 0.387665619842328 K255A-C262A 0.387327314322512 W297A-V317A 0.386933921958003 I242A-G264A 0.385002670966004 Q47A-F125A 0.384623420119331 Y180A-V308A 0.384217980616427 I246A-A261A 0.382837753161839 L54A-V133A 0.382347973347209 A118A-D310A 0.381850716104295 L243A-A261A 0.378278880951121 A29A-A120A 0.377108715617203 L198A-L259A 0.376375912349977 L58A-V133A 0.373097860377585 L296A-V317A 0.365143824913061 V50A-L129A 0.361904666422441 I28A-T205A 0.358865799410233 I287A-M328A 0.358459661368598 T300A-C312A 0.357860793265614 V134A-S139A 0.35677809879814 T24A-L208A 0.356259082174976 A118A-L176A 0.355991719611398 K303A-G316A 0.350055625773364 V116A-Y180A 0.349729567861751 L114A-A172A 0.349586412123204 V299A-V317A 0.34893626044615 G34A-M200A 0.34622081508719 C56A-T137A 0.34586187878305 Y32A-M44A 0.345217627944748 V270A-V317A 0.344625556428843 G244A-V289A 0.343453422417166 G164A-I323A 0.342996970654053 T51A-G113A 0.341920118248134 Y140A-G164A 0.338649127493288 C109A-T137A 0.337631286417073 K303A-P311A 0.337558889169282 M23A-V50A 0.336433635386711 F22A-C56A 0.334945001697309 V299A-D309A 0.33321953294429 G33A-M200A 0.333152135936313 I28A-I110A 0.331801338144594 L30A-L45A 0.330252202103888 G164A-A267A 0.329465734728049 V299A-G316A 0.327827147134489 G33A-V41A 0.327523900449859 T111A-T273A 0.324849786366133 V55A-G105A 0.322558276659731 L119A-R124A 0.321826268387348 L119A-D309A 0.321292151133147 T273A-G325A 0.321062299187396 G204A-A265A 0.319974440525143 G34A-V201A 0.316833270691021 A108A-L136A 0.315520648634436 G27A-G117A 0.313333658834883 V112A-L136A 0.31327003743529 G248A-A291A 0.311376651289032 G105A-G113A 0.31090191843617 A29A-M44A 0.310364076007919 G248A-G298A 0.309944495529279 A239A-A291A 0.309503442743471 Y140A-V167A 0.308673700534504 L302A-G316A 0.308435716071628 F31A-T205A 0.30685573801523 G34A-A120A 0.306805916543869 G244A-G271A 0.306117773921234 V112A-V132A 0.305778087858485 L176A-V314A 0.304436541678095 I246A-G264A 0.303952672791107 A239A-S263A 0.303517973798411 I242A-G268A 0.301764798192096 M44A-D313A 0.300889237502614 G268A-I287A 0.299592257871436 W245A-G295A 0.299318401642944 G113A-G204A 0.297653354999481 G117A-H168A 0.297118354917428 W245A-V290A 0.296116253240158 L243A-V289A 0.295899928084794 G295A-V317A 0.295890202720763 M44A-V116A 0.295876518730351 P26A-T51A 0.295197859273063 T205A-C312A 0.293865522198237 L19A-W212A 0.292602422212957 A241A-I287A 0.292173588507496 L176A-D309A 0.292133951033549 Y32A-V112A 0.291891235888094 V20A-I110A 0.291295475638515 A118A-G178A 0.291211421316635 C109A-I169A 0.291072873018134 G268A-G319A 0.290457555649829 D310A-F315A 0.290428844549445 A172A-K303A 0.290289089574689 R124A-L129A 0.289643990778256 G113A-A174A 0.289127447898278 I242A-A267A 0.289021850705548 A171A-G316A 0.288422008904142 M23A-G211A 0.288078903848562 L302A-G319A 0.288040761057549 G113A-L136A 0.286771604269899 T111A-T137A 0.286735596766846 C56A-T111A 0.286532812013101 T238A-C321A 0.284662173384017 H168A-G295A 0.284111312992857 I28A-V48A 0.284036875055622 V270A-V320A 0.283614666804253 V314A-V320A 0.280932579071298 N170A-C326A 0.280634607420025 A241A-L285A 0.280200012410938 N170A-C321A 0.278994823599938 V272A-V324A 0.278005389858946 T111A-W212A 0.275140852245796 I25A-V48A 0.275106076588955 K303A-C321A 0.274755416042404 L119A-G175A 0.274602670073725 A239A-A265A 0.273943910496214 G175A-D309A 0.272846619801015 G268A-L296A 0.272578753014629 V55A-L136A 0.271745276068041 V166A-C321A 0.268397155255512 V270A-V324A 0.268079135845446 G204A-S263A 0.267750591411938 C109A-F131A 0.267648453179971 P199A-S257A 0.266900160584808 G295A-C321A 0.265564246499339 P26A-P199A 0.265223528443224 A261A-G295A 0.26515626225298 G113A-A120A 0.264450123099785 L243A-G264A 0.262877728848984 T273A-G322A 0.260922414472073 T24A-C56A 0.260453217352982 G244A-W297A 0.259135615383844 A171A-H318A 0.259024813445325 A237A-I287A 0.257390076740057 A241A-I266A 0.256851927562504 I115A-A174A 0.255460251290014 D310A-V317A 0.254851875325716 G260A-G298A 0.254501541619305 C109A-N170A 0.254234877569993 V167A-V270A 0.254104645235786 I25A-F52A 0.253813342289003 Y209A-G271A 0.253523208424063 W135A-G178A 0.2535180284797 T234A-L269A 0.253073957301758 G298A-K303A 0.252906060146453 G164A-V270A 0.252559119579013 F202A-C312A 0.252440389292769 A206A-A261A 0.251775124462718 W135A-A174A 0.251608615670071 V317A-C326A 0.251400116753807 L54A-G113A 0.251097268590649 W59A-T111A 0.250044295703756 G163A-H168A 0.249963908873142 V201A-D313A 0.249691685694067 G117A-D309A 0.249271970742305 G33A-M44A 0.249183195583706 V116A-G175A 0.24871753230754 Y209A-S263A 0.248531537442592 C262A-V314A 0.248417968827908 M23A-I110A 0.247502134642843 T300A-P311A 0.246956086863583 T24A-T111A 0.246875856603452 F22A-G27A 0.246159942682543 I28A-M44A 0.244436504695996 A237A-L285A 0.244244874756605 G204A-L259A 0.2423676007115 T203A-L259A 0.241891303359631 E249A-G295A 0.241773185947988 G264A-W297A 0.240002331157696 L208A-T238A 0.238932437723282 G105A-L136A 0.238543131452671 L54A-G105A 0.237975082060763 W297A-V320A 0.235770438791016 L269A-G295A 0.23565315837521 G163A-W212A 0.235570154157672 T165A-G322A 0.234882283171412 G164A-I169A 0.234798179811063 A29A-L45A 0.233583999396291 I110A-V167A 0.233386410455212 L252A-T300A 0.233167038619085 V236A-V272A 0.233012395676602 H168A-W212A 0.232907351151652 W297A-G316A 0.23283591929616 W245A-W250A 0.232653438211295 L35A-T205A 0.232229463019213 G113A-N170A 0.231922703394802 A239A-L285A 0.231663964471096 W245A-G298A 0.231475237475388 L21A-T49A 0.231447483871241 Y180A-D310A 0.23083015881334 Y32A-D310A 0.230514151459631 V290A-I323A 0.230343878370851 V167A-I323A 0.230102143796655 G248A-L296A 0.229974691402853 A53A-A108A 0.229936612546428 L30A-T49A 0.228171745505853 T49A-T111A 0.227209328155959 W245A-S263A 0.226768151422983 G292A-G319A 0.226165429327507 I246A-G260A 0.225788081215152 G27A-I207A 0.225068546752 V270A-D313A 0.224285575539085 L293A-G316A 0.224201372434878 A120A-C312A 0.223352805176713 S43A-F125A 0.221870308817976 W212A-G268A 0.221772073112714 A294A-V320A 0.221181113409422 G268A-G316A 0.220694999530385 V48A-V116A 0.220422442844864 M44A-R124A 0.219034902307645 C312A-C321A 0.218337712443206 V317A-G325A 0.218090362684814 V132A-G175A 0.217759584273394 G271A-G316A 0.217497737293701 P26A-Q47A 0.21621146421774 G33A-L42A 0.216015005376928 G175A-D310A 0.215950046806699 T203A-L258A 0.215788215731354 Y32A-H318A 0.215336017629085 G113A-L129A 0.214561516136332 A172A-V317A 0.214325651836258 M23A-V201A 0.214298227012538 P26A-T49A 0.213353245644238 C56A-I130A 0.213330706400656 K303A-H318A 0.211940251020303 I173A-V317A 0.211049012968351 T51A-G105A 0.210929508888486 S263A-L296A 0.210885289367241 V166A-G319A 0.210339155590575 I266A-C312A 0.210248188544712 V201A-L258A 0.210243723110016 I25A-C56A 0.209888423125959 G260A-W297A 0.209785102837017 L21A-G211A 0.209772653710327 M23A-V167A 0.20975924203938 A108A-H168A 0.209545683990124 A29A-T49A 0.209514332916671 F107A-G211A 0.208774734649693 T165A-I323A 0.20846853116178 G211A-T273A 0.208273201934522 V270A-G316A 0.208152768401249 T51A-G117A 0.207682041698321 I242A-V289A 0.20731913894628 I110A-G163A 0.206555905823884 L30A-T46A 0.205992242605385 T49A-L129A 0.205602625703703 I173A-C326A 0.205590345547366 T238A-G260A 0.205549742313963 W135A-I173A 0.205015639546648 T111A-G322A 0.203861546502023 L198A-L258A 0.203597124332818 I110A-T165A 0.203380956292689 M23A-V270A 0.203213953429926 A118A-D313A 0.202513024518468 A174A-H318A 0.202494942335412 P256A-C262A 0.202486566291338 L302A-V320A 0.201555095615944 F107A-A171A 0.20070161510875 V272A-L285A 0.199939240670849 G264A-L296A 0.199831842294564 N170A-G178A 0.199704707923893 G117A-V128A 0.199278320261659 P26A-G117A 0.198901656428202 L243A-G292A 0.198775483885338 G105A-T137A 0.198683457375994 I110A-T205A 0.197810140267999 V272A-A284A 0.197752215095212 G211A-C262A 0.197023871559727 T46A-L129A 0.196529377514997 I207A-T238A 0.196348420888963 I110A-T273A 0.196213550874518 L21A-T111A 0.196097204902154 F31A-V48A 0.195823225716325 W135A-G175A 0.195800958524688 Y32A-V317A 0.195679324044989 V235A-L285A 0.195399163934485 W245A-G260A 0.194905868902582 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