[Wed Aug 24 17:55:08 2016] >Input file: TM2ONKATD.pdb >TM segments: C7-33_C47-73_C83-125_C127-147_C184-207_C230-251_D7-35_D47-73_D83-111_D127-147_D185-207_D230-251 MAIDEN score = -133.2675 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- P98C-S198D 10 S198C-P98D 10 V109C-G114C 10 V28D-A234D 1.90550677279431 V28C-A234C 1.90462581444503 V125C-A207C 1.88893780670188 L9C-P71D 1.81126252547306 P71C-L9D 1.80244083420138 P23C-L106D 1.76065361989023 L106C-P23D 1.74214362289836 A147D-I185D 1.4531687978107 A147C-I185C 1.43980898667434 L107D-I206D 1.23117326261339 L107C-I206C 1.21321760362529 F110C-I116C 1.19348571481257 I97C-I237D 1.1331481018032 V125C-I206C 1.10543878346977 I237C-I97D 1.08093056469343 H99D-F200D 1.06292546536365 H99C-F200C 1.05858552587484 I104C-V205C 1.01382013190636 I104D-V205D 1.01157944851477 I60D-V132D 1.01139358183994 V109C-L115C 0.998364891308938 I90C-L21D 0.9909276123638 S61C-L136C 0.990808310062215 F137C-V20D 0.985616224256176 F110C-V125C 0.976953379041135 I60C-V132C 0.959356849763973 V20C-F137D 0.958603446283232 L72C-S13D 0.950832851478494 A134D-V203D 0.941861309653952 I131D-I206D 0.939426919173888 L21C-I90D 0.904541622975929 A134C-V203C 0.900821451368618 S61D-L136D 0.894079504133074 S13C-L72D 0.85818234886473 V138C-R195C 0.852084552394839 V138D-R195D 0.848299670209652 V96C-R195D 0.848246625229444 R195C-V96D 0.831393914964428 T100C-F200D 0.830112241991549 I90C-L17D 0.810977026032439 L17C-I90D 0.810219784078573 I131C-I206C 0.809272143230281 L59C-W193C 0.80866458996997 L59D-W193D 0.802680611339575 P71C-L12D 0.762076634312127 F124C-P129C 0.756989632843394 F200C-T100D 0.755792020761767 T100C-A202C 0.752647187026501 I104C-M230D 0.749547488994408 V138D-E199D 0.723711471395218 V138C-E199C 0.71942257974693 V66C-Y143C 0.718619079659615 I90C-V20D 0.708401052103056 Q31C-M230C 0.700749479988273 G69C-I16D 0.695291142534021 L17C-V91D 0.694029528141328 L12C-P71D 0.689734125994431 V91C-L17D 0.688984572384706 I16C-G69D 0.680733669843844 G130C-V203C 0.679906492405243 T100C-V205C 0.678091136493209 L106D-A134D 0.673969938854755 A65C-L136C 0.671771066130447 A65D-L136D 0.660625671898682 V20C-I90D 0.659528148496516 L106C-A134C 0.65782238314458 I142C-G188C 0.647661264766421 I142D-G188D 0.644678757631015 T100D-A202D 0.643130358847895 T100D-V205D 0.638910898142974 A57D-L128D 0.637213529168346 F68C-I15D 0.628897749483451 M230C-I104D 0.627859558829371 F110D-G130D 0.626988645031016 V109C-I116C 0.626210049800719 F110C-G130C 0.62341734308393 G130D-V203D 0.617391985363412 M135D-A202D 0.616300074415967 G117C-F124C 0.61338068772166 M135C-A202C 0.612205515352407 G103D-V204D 0.612202924740114 A102D-F137D 0.609089642234835 A102C-F137C 0.609077475923348 R195C-P98D 0.605995855496614 I142C-M191C 0.605271690929719 A102D-V138D 0.603721487814295 Q31D-M230D 0.601941882717835 A73D-I144D 0.601072356963684 V66D-Y143D 0.600065134879816 A102C-V138C 0.598458985684881 I142D-M191D 0.595388702894907 A26C-V109D 0.589383073919659 V24C-A234C 0.585422542487408 P94D-F137D 0.580637044341188 G69C-Y143C 0.578282630978619 S13C-G69D 0.577572663789665 Q146C-H184C 0.577334454275984 V24C-I237C 0.575812329444058 Q146C-I185C 0.575638688468787 I190C-R248C 0.575196522197913 P94C-A102C 0.57362179461941 Q146D-I185D 0.573121129270755 P94D-A102D 0.572916534509563 I15C-F68D 0.569136271109875 A65D-Y143D 0.56762177064588 G69C-S13D 0.566593081102577 F110C-F124C 0.566108144121199 P94C-F137C 0.563903105718779 G69D-Y143D 0.557442429586508 V109C-A26D 0.555261810544491 V24D-I237D 0.55310202395871 T27C-V108D 0.550986974081498 H99C-V138C 0.54930443308129 H99D-V138D 0.548413623439893 V138C-A202C 0.548412590933672 A73C-I144C 0.54360089185797 V138D-A202D 0.538266406296575 F124C-A207C 0.534690478469327 S139D-G196D 0.533964352207305 V108C-T27D 0.53143405324011 S139C-G196C 0.529195852994882 Y143C-I185C 0.528664095997812 Y143D-I185D 0.522673254689679 Y55C-I197C 0.520485796595931 T62C-Y143C 0.514513833838112 V24D-A234D 0.510936064204833 V138C-G196C 0.508096401256411 V101C-A202C 0.507601563008423 V138D-G196D 0.505205886195069 G103C-V204C 0.504687058246873 V101D-A202D 0.501526397276986 V108C-A26D 0.49646803656224 L72C-I144C 0.492896069060585 Y55D-I197D 0.490555665878053 T62C-W193C 0.486087466580688 P98C-R195D 0.481839888242853 P71C-A10D 0.47916577157332 I97C-L236D 0.477582162790646 P94C-E199D 0.476300696680587 E199C-P94D 0.472107074975074 E88D-P94D 0.469088842202318 E88C-P94C 0.468992787467272 L72D-I144D 0.467290077054511 V95C-R195D 0.466675926640789 V95C-S198D 0.464184266738205 L106C-I206C 0.463837342609085 P94D-E199D 0.46305253003559 S61C-M135C 0.461864242846456 I131C-V203C 0.461850902500959 I131D-V203D 0.46083577991206 S198C-V95D 0.460586818482857 P94C-E199C 0.459993144721104 A10C-P71D 0.458580411501464 A26C-V108D 0.457088787337954 K123C-P129C 0.455150470197323 V28C-A233C 0.453437425596254 M135C-R195C 0.451479455661675 T27D-A233D 0.449927808855688 M135D-R195D 0.448397105486092 G111D-I206D 0.448055626779761 M135C-E199C 0.444899636833883 M135D-E199D 0.44483553280496 L106D-I206D 0.443056054721901 L122C-P129C 0.437422974701112 T27C-A233C 0.435325642555973 T62D-W193D 0.43448980845951 V66C-A189C 0.433527267919903 A105C-M230D 0.430301154929411 R195C-V95D 0.42983459141186 F19C-F137D 0.428695273579011 F68C-L12D 0.428610699748607 F110C-L115C 0.427307384523459 T62D-L136D 0.426831904554755 T62C-L136C 0.426266292819117 L12C-F68D 0.425632533805202 S61D-M135D 0.424530088470873 P98C-F200D 0.421614611103096 L136C-R195C 0.420793947290591 L136D-R195D 0.420430612357443 I64C-G69C 0.420061729205634 L9C-L72D 0.41900809338889 I64D-G69D 0.417777579998678 L107C-V125C 0.415715581797657 F200C-P98D 0.414737151021817 L236C-V101D 0.413455128634512 F110C-S118C 0.412939973977299 P98D-E199D 0.409804007222417 L72C-S14D 0.409713474925704 F137C-F19D 0.408992454019343 V96C-L241D 0.40817929902942 V28D-A233D 0.406987648749745 I64D-L136D 0.404741134683815 F110C-K123C 0.403696349176542 P141D-Q146D 0.4036620184806 P141C-Q146C 0.403605061914157 G111C-S118C 0.402598634680322 G69C-V140C 0.399947969144229 P98C-E199C 0.398337355790415 L72C-L9D 0.394899165498165 I131D-A207D 0.393185530654019 V108C-L115C 0.392146348270707 V96C-W193D 0.391367981198966 I104D-I206D 0.390978323947375 L72C-A10D 0.388747718553037 A10C-L72D 0.388600168282663 A102C-V24D 0.38711393572452 H99D-S198D 0.385971796996511 V24C-A102D 0.385434949193972 G69D-V140D 0.384549347828167 L241C-V96D 0.38436985459012 V108C-P23D 0.383862160963268 A65C-T70C 0.383514525240695 P23C-V108D 0.383094584260413 A65D-T70D 0.382119467178494 V101C-L236D 0.38175311467869 T54C-I197C 0.380382819031615 N34D-P231D 0.37887622274278 H99C-S198C 0.378610104226123 I16C-F68D 0.37791540114063 L106C-V24D 0.375857574958011 V109C-L22D 0.37520073823923 W193C-V96D 0.374615176906968 I60C-M135C 0.374567028560024 I131C-A207C 0.373657192324467 I104C-I206C 0.373504799037544 S61D-V132D 0.372441166514804 H99D-S139D 0.371437068528224 V101C-A234D 0.371273936123419 V108C-S113C 0.370084598061337 V91C-V140C 0.369352099585099 V24C-L106D 0.36906773875203 I97D-A102D 0.368420518246491 I97C-A102C 0.367528371468318 H99C-S139C 0.366117271281727 F124C-I131C 0.364895572240937 V47D-V232D 0.362635111243077 A25C-A233C 0.362094736618167 V66D-A189D 0.361910788167867 W193C-P98D 0.361194457193151 V28C-L238C 0.3603117664214 A26C-A233C 0.359334721954574 L107C-G114C 0.357969606555508 S14C-L72D 0.356985676143001 Y56D-V133D 0.356837115441042 Y143C-V20D 0.356417219934962 P98C-P141C 0.356319641009462 A57D-A194D 0.355369621903786 K84C-A10D 0.355158600492069 Q31C-A234C 0.354545919378832 A134C-I206C 0.353910950214606 I64C-L136C 0.353693620055511 A26D-A233D 0.352516853837103 L22C-V109D 0.351578757075977 A10C-K84D 0.349884929922998 P23C-L107D 0.349775967330631 V91C-V20D 0.349098659212282 A25D-A233D 0.348777416989125 V47C-V232C 0.346571844666288 L128D-A207D 0.346356002568798 G117C-K123C 0.345948072698513 L107C-V205C 0.344140354897778 A102C-A25D 0.343460440302294 Y56C-V243C 0.343080413760888 V20C-V91D 0.342948469052758 P98C-P231D 0.341629190413486 Q31D-A234D 0.341552286999919 P98D-P141D 0.341159762905846 I97C-S198D 0.338879644025671 A134D-I206D 0.338846392089214 S61C-S139C 0.338539070186766 L136D-G196D 0.338037075968319 V109C-M135C 0.336433635386711 F68C-I16D 0.336155554688267 I60D-M135D 0.336127781291103 L136C-G196C 0.335356710171965 H184C-S192C 0.33469471010249 A58C-L136C 0.334197643219684 D92C-V245D 0.333503871679229 A65C-Y143C 0.333003714951287 V245C-D92D 0.331758795415145 A25C-A102D 0.331472427827589 V20C-Y143D 0.331282712274422 V109D-M135D 0.327788641017192 Y143C-M191C 0.327291518631853 Y143D-M191D 0.327267452683544 A57C-L128C 0.327116970891644 P94D-N145D 0.327063783078009 S113C-P121C 0.326575820802801 P94C-N145C 0.326203832880403 A58C-M135C 0.325409719567718 A58D-G130D 0.322515663607248 P98C-W193D 0.321933942249489 P141C-S13D 0.321400686988152 A57C-A194C 0.320769891628885 A134D-S198D 0.319134092043336 A134C-S198C 0.31831413206737 T54D-I197D 0.317232341566983 L128C-A207C 0.314641217442758 Y56C-V133C 0.314244733766212 V20C-L93D 0.314059075903934 G130C-G201C 0.31405100795687 I104C-A233D 0.313901024853118 G188D-G196D 0.313459149709185 G188C-G196C 0.313153853214116 F124C-I206C 0.313056468216078 A57D-S198D 0.31068482425476 V28D-L238D 0.310395016890541 I104D-A202D 0.310277184132381 Y56D-V243D 0.310275267828277 A58D-M135D 0.310226367693113 G69C-H184C 0.309925927917377 V66C-I185C 0.309547982139636 G111C-I116C 0.308467789274932 V96C-I197D 0.30765444012712 A73C-Q146C 0.30685710145757 V101C-I237D 0.305517491982997 S61C-V133C 0.304948802774677 L93C-V20D 0.303871811994728 I237C-V101D 0.303645880981119 S139D-W193D 0.303250102392526 S139C-W193C 0.303109689069296 T70D-I185D 0.301159467187196 I144C-G188C 0.300136961125538 A127D-I206D 0.300046182979052 G111C-F124C 0.299553925738816 I197C-V96D 0.299252344664164 V95C-P141C 0.29882819362219 I144D-G188D 0.298071001347729 S61D-V133D 0.297935785727205 V28C-I237C 0.297418084242773 A73C-S14D 0.296984854996352 S61D-W193D 0.296870905191327 A65C-I16D 0.296490086058002 P23C-T100D 0.29633212627415 L52C-A57C 0.295441669851903 T100C-P23D 0.295379654743697 L52D-A57D 0.295028738723843 L72C-L12D 0.294368310529477 I104C-A202C 0.293332162126868 S61D-P141D 0.29303793173503 L12C-L72D 0.292055053413923 V66D-I185D 0.292044959894986 F200C-F200D 0.291410188554216 A234C-L239C 0.291409697422984 L21D-A26D 0.291263753776314 T62C-P129C 0.29117033792145 V28D-I237D 0.291043380239965 L106D-A202D 0.290795057754152 L21C-A26C 0.290278601039095 V66C-I144C 0.290059312634953 S13C-P141D 0.289997582753156 A234D-L239D 0.28958547164859 A58D-L136D 0.288823636023921 S240C-I97D 0.286137052170748 T100C-P231D 0.285291358760972 L236C-I97D 0.284585175571129 G114C-G130C 0.284478959251895 G130D-G201D 0.284347983013641 V101C-V205C 0.284300000832417 V66D-V186D 0.284020289474382 L241C-A246C 0.283770810150363 V66C-V186C 0.283273631092404 S13C-F68D 0.283224293099898 L63C-A189C 0.283063213098455 V101D-V205D 0.282948492737949 L241D-A246D 0.281469380626262 D92D-M135D 0.280917050033382 G89C-N145C 0.280794160659638 T62D-Y143D 0.279812657109682 V108C-V24D 0.279738537671896 L63D-A189D 0.279393124185043 T70C-I185C 0.279247708341963 G130D-G196D 0.279127006930153 Y56D-R195D 0.274544476566582 G114C-L30D 0.274416531767659 T62C-V140C 0.27420734164023 A58D-I131D 0.273206574113483 A65C-G196C 0.272939503549201 V28D-I235D 0.272468745844213 V95D-P141D 0.271688782006396 A233C-A105D 0.271489900350163 F68C-S13D 0.269789258966761 G89D-N145D 0.26958482918551 S61C-W193C 0.269211738221727 A102D-A134D 0.268899075791837 L107D-V205D 0.267953376242632 L107C-P23D 0.267707705709718 G111C-K123C 0.267576770551401 M230C-A105D 0.267107777813217 L17C-V87D 0.266978526087929 Q146D-A187D 0.266645723966297 A58C-I131C 0.266198693737954 G69C-S192C 0.265169604062482 A102C-A134C 0.264352883606219 P231C-P98D 0.264240495866503 A58D-R195D 0.263448989663249 S61D-G196D 0.263256934515947 Q146C-A187C 0.263119982197377 I16C-A65D 0.263065349085061 H99C-L236D 0.263020193079185 L106C-V133C 0.262726787905674 A73D-Q146D 0.25956499420792 V87C-L17D 0.259183519205264 V51D-M135D 0.258760397555093 S112C-F119C 0.255384191145215 L106D-V133D 0.254499261371286 V91D-V140D 0.253992454156524 A58C-I197C 0.25371435752611 V91D-I144D 0.253230102150094 H99C-V203C 0.25275711592986 Y56C-R195C 0.25258919132243 A26C-A105D 0.252535518476935 I197C-P98D 0.251830494454693 S61C-V140C 0.251597802927916 P98C-I197D 0.251576847053592 S14C-A73D 0.249299847789221 M191D-R248D 0.249254009419695 L93C-S240D 0.248439900305658 G103C-I206C 0.247298056898898 E199C-V204C 0.246934906050093 T62C-Q146C 0.246792231863544 G69D-S192D 0.245522407523318 M135D-I197D 0.245507317079336 M135C-I197C 0.245110616570996 V24C-V108D 0.245068128165505 E199D-V204D 0.244430696033755 D92C-M135C 0.242991473768238 A57D-I197D 0.241138769354275 G103D-I206D 0.240811683864462 T70C-Y143C 0.24076713532272 S61D-S139D 0.239732030409963 L236C-H99D 0.238639977415862 A7D-L12D 0.238245395721204 V138C-V20D 0.237124269068626 F19C-G69D 0.235702157613647 V28C-I235C 0.235640300358981 F244C-V96D 0.235262930421196 V91C-M135C 0.2341306810393 L17D-P23D 0.234097350562226 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