[Wed Aug 24 17:24:15 2016] >Input file: TM2RH1ATD.pdb >TM segments: A34-60_A68-94_A104-131_A150-175_A195-223_A272-298_A307-329 MAIDEN score = -118.4041 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- A78A-C116A 1.46721816591071 I201A-I294A 1.08270700583745 V86A-W109A 1.01132098555336 C125A-M215A 0.980864656445176 P168A-W173A 0.970582852742407 E107A-I169A 0.939741504362186 V48A-D79A 0.907613238000789 C285A-I314A 0.881972121926069 I47A-L80A 0.844862067103458 I121A-L212A 0.806863690897867 W286A-G315A 0.764355561759364 A85A-W109A 0.762864241462916 W286A-I314A 0.754803726445425 V292A-Y308A 0.74606205756912 I278A-I325A 0.731622774443231 F289A-Y308A 0.726837615025651 T123A-V157A 0.713056096749309 I112A-S165A 0.709765193456189 L115A-S165A 0.684444749436359 F71A-I154A 0.672152399376595 P288A-L311A 0.671635633497427 F71A-I127A 0.666376768692752 W286A-L311A 0.663324638738073 I58A-T73A 0.660551639793685 V86A-I112A 0.653686529221298 I121A-F208A 0.650775286481876 V48A-L84A 0.637512332802469 F289A-G315A 0.622160223285385 D79A-C116A 0.617191562330204 V54A-T73A 0.611092984315272 F71A-A150A 0.601223258465029 T274A-Y326A 0.594009263755396 C285A-L311A 0.586689278098305 G83A-P88A 0.579204022178649 V129A-Y219A 0.578271968941312 A200A-I294A 0.578226186934652 Y70A-A150A 0.572312739283053 V48A-L80A 0.568748670781132 I201A-V297A 0.566072427806792 L53A-P323A 0.564566444152113 I43A-V317A 0.550822332124021 W173A-Y199A 0.546186173295325 I121A-S207A 0.544293636185283 T123A-I154A 0.539156822886881 L115A-W158A 0.537719063469599 N51A-C77A 0.532728268983817 F223A-L272A 0.527913339807058 S161A-S207A 0.524368858283205 A200A-V297A 0.52024235117123 A128A-V222A 0.513328666673698 I201A-N293A 0.513241282515642 F89A-W109A 0.509533344170434 V52A-A76A 0.50364401538695 A119A-W158A 0.499895287297296 S204A-I294A 0.498857244774805 F71A-T123A 0.498768168724569 T274A-I325A 0.494799194455645 F289A-L311A 0.481023078357779 W286A-N312A 0.480699854097965 V52A-L80A 0.478290664810851 M82A-V87A 0.4770356230666 I205A-V210A 0.470762898717084 F108A-D113A 0.466903689112325 L80A-S319A 0.461966318447192 I47A-M82A 0.458540452850668 F71A-L124A 0.457892365849098 V54A-A76A 0.457236908425269 Y174A-A200A 0.455986544265454 Y70A-R151A 0.450958609018239 R131A-L272A 0.449425246397666 T118A-F208A 0.44680252825177 E122A-T164A 0.444586747973397 Q170A-Y199A 0.442420520677873 L124A-M215A 0.43505870460758 V87A-Y316A 0.431301729131953 S111A-F166A 0.430782475285898 R131A-V222A 0.427517069363217 I121A-F282A 0.424760779823552 V126A-R131A 0.424303948661698 F282A-G315A 0.414508391745414 L115A-F166A 0.414012702209227 C125A-D130A 0.411280934972792 A85A-D113A 0.410498898396976 A128A-V216A 0.404930118636898 I47A-F321A 0.403491480322342 T164A-S207A 0.400885342394376 I55A-K60A 0.400355334611626 I169A-Y174A 0.398923855437977 C125A-R221A 0.395723672086262 A78A-V117A 0.393213422830849 I58A-Y70A 0.390314062435485 S74A-T123A 0.389768475431366 W313A-N318A 0.38761166145251 V44A-P88A 0.386276264769091 P288A-N312A 0.385145242143038 D79A-V117A 0.384250964981012 I277A-I325A 0.378234675108296 C125A-P211A 0.376352833968473 A57A-A76A 0.373801152762447 W286A-N318A 0.373427418666611 L115A-T164A 0.371087703725411 I43A-G320A 0.370481764630529 W173A-N196A 0.369971294954456 S203A-P211A 0.364749215689324 G50A-C327A 0.363106457344282 D113A-Y316A 0.360760048898678 V216A-R221A 0.359864611853477 L75A-A119A 0.359688574554798 M82A-S319A 0.357804151167157 C77A-T118A 0.356445756585691 C106A-R175A 0.355997710669612 P288A-N293A 0.354656460827 G162A-L167A 0.350678537671067 Y174A-V297A 0.349546393735257 I47A-G320A 0.348680121578225 Y199A-S204A 0.348532812402691 N69A-P323A 0.347864277793246 P168A-S204A 0.346803115452548 T118A-S165A 0.343694600444876 M36A-W313A 0.343490116394246 E107A-C116A 0.343365072460983 L212A-T283A 0.342166786501205 Y219A-G276A 0.339859980249561 S111A-I169A 0.339465097443741 V54A-M82A 0.336433635386711 M40A-A91A 0.335443276709828 F71A-C116A 0.332119705024636 Q197A-H296A 0.331907522274109 S165A-H172A 0.329071020028058 V52A-Y326A 0.324352240029888 T110A-I169A 0.321046750219394 L124A-M279A 0.320609114631664 S204A-P288A 0.315312652834481 V44A-L84A 0.313677793070147 M82A-Y316A 0.313507883540917 P288A-W313A 0.310953126645594 T164A-S203A 0.310846477655915 S74A-S120A 0.310523705089583 I112A-F166A 0.309342533945804 C106A-H172A 0.308604587205867 G280A-G315A 0.306979609911339 T110A-T164A 0.30505338766399 C77A-R151A 0.303571302177811 L75A-E122A 0.300404541829866 S204A-F290A 0.299256511816714 I55A-C77A 0.297585230441019 C125A-V160A 0.297363836636174 S41A-G90A 0.297356063080492 V117A-W286A 0.295814736432899 V48A-G320A 0.29473478200172 L75A-L124A 0.294031488844413 T164A-P211A 0.293692849836339 A78A-A119A 0.293254807850707 P168A-S207A 0.290627488999517 S120A-G315A 0.290497446477364 V206A-P211A 0.290360445747453 E122A-G162A 0.289784963989507 C77A-M82A 0.28920575821121 S220A-G280A 0.289003188985451 P88A-T110A 0.288855682505116 C125A-I214A 0.288828228616221 V117A-M215A 0.285670204133103 T73A-W158A 0.285643643319385 M82A-I112A 0.285087827366901 C116A-I314A 0.285073064232575 F89A-E107A 0.282729502528435 F208A-F290A 0.282564848143824 A128A-M215A 0.282165893418689 F71A-V126A 0.281944464530907 I47A-V317A 0.28166427408969 N51A-C327A 0.279380151419083 S111A-H172A 0.278957111790319 P88A-W313A 0.278813807883457 V129A-M215A 0.278158220784441 V81A-C116A 0.277474882040542 G50A-C77A 0.275033675646204 T274A-S329A 0.270629187768302 N69A-C77A 0.269308125665638 C285A-N322A 0.268649635707257 T68A-D130A 0.266291162274669 I47A-C285A 0.265848999995395 C125A-M279A 0.265548338056102 C125A-L212A 0.265473450267892 A85A-I112A 0.263929011039348 E107A-P168A 0.263524882489786 N196A-S203A 0.263277073982189 G83A-D113A 0.262140745132098 Y174A-Q197A 0.261097165228902 G90A-G315A 0.260567606661817 S204A-H296A 0.259979993575554 T68A-C125A 0.259050504486326 L53A-G320A 0.257954682590023 L75A-G320A 0.257882863869686 R131A-M279A 0.257756934797639 A85A-A91A 0.257576873386574 C116A-N318A 0.257513235657568 W313A-G320A 0.256053953728217 I121A-P211A 0.255692253754366 E122A-L212A 0.25452439693882 N51A-S74A 0.252755173544955 C285A-V317A 0.251204899976164 L75A-T123A 0.250501908701468 P168A-Q197A 0.249900248389317 L75A-S319A 0.249107186984358 S165A-Y199A 0.248517421090892 Y70A-I154A 0.248314142790357 Y70A-D130A 0.24488218687233 S120A-N318A 0.243145617008437 A78A-Y316A 0.241168012697912 D79A-Y316A 0.241051349082931 E122A-V160A 0.240466073049884 A78A-L115A 0.240422510609947 I278A-F321A 0.24029518287802 V54A-P323A 0.239913106676354 E122A-F208A 0.239614046669541 T73A-L124A 0.239269890641189 D79A-L84A 0.23864622543709 A57A-Y326A 0.238368187961856 L124A-T281A 0.236862441313056 V81A-V86A 0.236526153382554 G50A-N318A 0.234458779728661 T283A-P288A 0.232431267157912 C125A-G276A 0.230263330051626 D130A-V216A 0.230232779647223 E122A-V210A 0.230217293542482 A78A-G320A 0.230033605924624 V87A-I314A 0.229676097011391 G315A-N322A 0.22788603981873 V126A-M215A 0.227338216441622 L75A-N322A 0.227312930653675 F108A-Q170A 0.227033938885478 P288A-N318A 0.226859367795748 C106A-Q170A 0.226342488435658 A200A-F208A 0.225614654496869 L80A-P323A 0.22380242560142 F166A-Y199A 0.223363378010049 C77A-E122A 0.222621617904025 L284A-N318A 0.222592678750502 H93A-T110A 0.218669628444129 D79A-M279A 0.218514507416887 I278A-G320A 0.21754902801773 V54A-D79A 0.216936673996962 D113A-V317A 0.21690419193832 S111A-S120A 0.215881975960722 C77A-F321A 0.215332335618255 E122A-T283A 0.215122663762684 C77A-N322A 0.214777710982457 V117A-M279A 0.214315491727438 I58A-C77A 0.214210053098557 N51A-L75A 0.213507105522813 S165A-P211A 0.213489795494819 G90A-L310A 0.212191422151527 S120A-A128A 0.211618648320854 M40A-H93A 0.211404979068604 V44A-L80A 0.211090301073027 T164A-W173A 0.210877484536576 Y209A-V292A 0.210566326328521 F290A-V297A 0.209087691998808 C116A-V317A 0.208091941422804 V157A-S207A 0.207983795758983 T56A-T73A 0.207303000670199 S207A-V213A 0.207141390442015 V48A-F321A 0.206778686864624 V117A-E122A 0.206248681591114 R131A-Y219A 0.205156867180528 T123A-P211A 0.204677292272572 C125A-T283A 0.203960148428345 W109A-V114A 0.20366302576364 L310A-N318A 0.203620015756864 Y316A-N322A 0.203521008441147 V39A-Y316A 0.20311209664917 G50A-L324A 0.20270447309605 M215A-L272A 0.202676104644888 T123A-T283A 0.20250112794061 T118A-P168A 0.202347298090782 M215A-L284A 0.202260117684073 G83A-S319A 0.201596580022254 T164A-H172A 0.200241116316419 T56A-N69A 0.198966386668534 Q197A-N293A 0.198965778310316 I112A-W158A 0.198441653796976 T73A-I121A 0.198213931571037 L115A-V160A 0.196260311150563 T118A-I169A 0.196242766016485 N69A-R131A 0.195896532206486 L45A-G83A 0.195708937640211 V54A-G320A 0.195142945141546 T118A-W286A 0.194040203463601 I214A-Y219A 0.193852305925277 H93A-W313A 0.193726904311233 M215A-L287A 0.193721059084809 D79A-G320A 0.192578516348899 L53A-C77A 0.192236488116604 Y199A-F290A 0.190187765107266 F166A-M171A 0.190175179789552 V129A-V218A 0.189788979270469 I72A-T123A 0.189164585541019 A46A-D79A 0.189057730022121 N69A-L75A 0.188922692413413 A46A-G320A 0.188501130168669 R221A-K273A 0.18844083946036 I55A-A76A 0.187666988498412 F49A-C327A 0.187589883405249 M36A-L310A 0.187035179930716 S74A-M279A 0.186162435911929 C77A-I112A 0.185939323117624 A85A-E107A 0.185550139292381 A59A-T68A 0.18495093643976 M156A-S207A 0.184942095278901 N51A-F71A 0.184636648598331 S203A-F208A 0.184580889217006 M82A-S165A 0.183716916202158 M82A-S161A 0.183565561843105 W286A-W313A 0.182903942660327 A76A-Y326A 0.182071790074206 V222A-M279A 0.181838185421128 P88A-S111A 0.181658245701461 Y199A-S207A 0.181567253594126 S165A-A202A 0.181139616579037 N51A-G320A 0.179871130922416 A128A-I278A 0.179854481209679 Y209A-W286A 0.179825206804911 G83A-I112A 0.179397639652285 S120A-Y209A 0.179229155336617 G37A-H93A 0.178664325347654 S120A-L155A 0.178652059250998 M279A-G315A 0.177433686233799 L80A-V86A 0.177132793755287 L42A-S319A 0.17676371767731 L124A-S161A 0.176484268629423 Y199A-F208A 0.176406191962672 V117A-F290A 0.175618257038489 K273A-R328A 0.175616616760807 Y70A-S120A 0.175427853233392 Y174A-S204A 0.175178996913856 Y209A-C285A 0.173872907139723 A57A-S329A 0.173818776475625 K273A-I278A 0.1736645957131 F290A-Y316A 0.173587472561984 A76A-N322A 0.173152436360308 L167A-S207A 0.173146921738714 S41A-Y316A 0.173041396528755 S41A-S319A 0.172440947998813 F71A-V152A 0.172422426074222 D113A-G315A 0.172304158199064 V114A-G315A 0.171701855349475 H93A-F104A 0.171390242143857 D79A-P88A 0.170853716907149 T110A-H172A 0.170274578186609 Y70A-C125A 0.169461685783112 L75A-M156A 0.169177284936755 A78A-S111A 0.168758635371304 S319A-Y326A 0.168705072794942 T73A-I278A 0.167986151889257 C125A-W158A 0.167974555829213 L80A-P88A 0.167565231262881 T164A-I201A 0.166583349856336 D79A-T123A 0.16650902843944 A76A-G315A 0.165401997855501 Q197A-A202A 0.164606792729469 L115A-P168A 0.163814880576806 A200A-I291A 0.163666276210875 I291A-N312A 0.163560999488626 T164A-A200A 0.163360275205306 C116A-G315A 0.162967626003525 V81A-I112A 0.162814791825832 C77A-L115A 0.162313410273471 W158A-L167A 0.16163549008298 I47A-I314A 0.161391118703792 D113A-T164A 0.160708531785214 V114A-F166A 0.160669774791001 C106A-Y174A 0.160169426668963 G90A-Y308A 0.160102444215668 T118A-P211A 0.159743808930867 L275A-P323A 0.158924921961731 C125A-L275A 0.158593660024924 H296A-V307A 0.158270024740967 Y70A-R131A 0.157298355277061 I277A-C327A 0.1571023926771 L84A-C116A 0.157003203488421 V87A-G320A 0.156666926229959 S41A-P88A 0.156103758238619 I205A-I294A 0.155804083750345 L53A-C327A 0.154996949623702 H93A-I309A 0.154809846163992 N69A-D79A 0.154263983560022 V295A-V307A 0.154050528050784 S111A-S204A 0.153934629044215 M279A-V317A 0.153870767401061 F282A-N318A 0.15357246890129 G276A-N322A 0.153139351257868 W105A-D113A 0.152322141088712 I121A-M156A 0.152177518846025 G83A-W313A 0.152175216724378 A46A-V81A 0.152073246766255 I47A-V87A 0.151996198697671 M82A-I121A 0.151500041031714 H172A-A198A 0.15131189735538 C116A-T164A 0.151153046848828 A85A-S319A 0.151133786359111 Y209A-M279A 0.150832600987215 P88A-G320A 0.150580147003714 I58A-N69A 0.150461941139193 I277A-C285A 0.15017580068821 D113A-W313A 0.149337373861133 I47A-V81A 0.149314227074418 A57A-Y70A 0.148757487751874 V218A-G276A 0.148071560121993 D79A-N322A 0.147510099442708 I72A-I277A 0.14702691333192 N69A-L272A 0.146973963685661 S207A-F289A 0.146890511631585 Y199A-V295A 0.146459834744593 L42A-Y316A 0.146455668256231 D79A-N312A 0.146191426603601 M82A-A119A 0.144359097872412 V210A-F217A 0.144221875821443 S111A-Q170A 0.144166745080342 S74A-V152A 0.144016262850658 G90A-W105A 0.143943950794643 S74A-L155A 0.143440017664179 T118A-A202A 0.142780984567621 N69A-S329A 0.142485054811255 T56A-C77A 0.141332034898652 G280A-I325A 0.141140056513571 G280A-F321A 0.141128137988406 I294A-L311A 0.141041586300048 D130A-M156A 0.141001691759705 A57A-I72A 0.140757530235885 G37A-G90A 0.140660666159758 M36A-G90A 0.140531679306498 S120A-F321A 0.140134997093682 R131A-A150A 0.140058963381268 E122A-S203A 0.139758456946979 I291A-I309A 0.139667862921932 Y70A-Y326A 0.139408722889989 I127A-I214A 0.139285611758553 A78A-V317A 0.139160135754184 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