[Wed Aug 24 18:20:02 2016] >Input file: TM2WITATD.pdb >TM segments: A64-81_A91-112_A178-197_A240-262_A280-294_A305-354_A363-381_A402-419_A452-473_A493-509_A517-534_C64-81_C91-112_C178-197_C240-262_C279-294_C305-354_C363-381_C402-419_C449-473_C493-509_C515-534_B63-79_B91-112_B178-197_B240-262_B280-295_B305-354_B363-381_B402-419_B452-473_B493-508_B517-534 MAIDEN score = -286.0293 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- N331B-W101C 10 W101A-N331C 10 W101B-N331A 10 L105B-P334A 2.02044713338232 L105A-P334C 2.0127613828488 P334B-L105C 1.94888805063321 I329B-I415B 1.90866613551673 I329A-I415A 1.90818420477873 I329C-I415C 1.90756607498171 I291C-I466C 1.70363482892228 I291A-I466A 1.69759136883115 I291B-I466B 1.69674265882622 I78C-T505C 1.69609988431341 I78B-T505B 1.67890080138252 I78A-T505A 1.65469968890155 I375A-L530A 1.25801221386291 I375B-L530B 1.2579159405406 I375C-L530C 1.25678972263633 V111A-A195A 1.25488788189259 V111B-A195B 1.21544365590444 V111C-A195C 1.2007546854989 L363B-I368B 1.16845455457334 L363C-I368C 1.16768663674706 L363A-I368A 1.1654203217464 T184A-Y340A 1.12627689551262 I104B-W371B 1.12602955704194 T184C-Y340C 1.12352438644405 T184B-Y340B 1.12097693519273 I104C-W371C 1.10532235081688 I104A-W371A 1.10212997978456 L341B-Q346C 1.09511763499043 Q346B-L341A 1.09460285817021 Q346A-L341C 1.08464656548221 P334B-R350C 1.00571602357689 F187A-Y340A 1.00300414753971 F187C-Y340C 1.00041180959709 F187B-Y340B 0.997932753842328 R350B-P334A 0.996700660311874 R350A-P334C 0.995280654087269 F103B-W371B 0.960121486012675 F103A-W371A 0.956529389785262 F103C-W371C 0.95299336861566 V75C-F80C 0.951420183702387 V75A-F80A 0.945988515415007 D97B-V327A 0.936231692382115 A372A-I523A 0.930609816897705 A372B-I523B 0.920980487093319 A372C-I523C 0.918344959144804 V320B-F416B 0.91648450672188 V320A-F416A 0.916476988552454 V320C-F416C 0.913449020676839 L254C-F465C 0.907253158646046 L254A-F465A 0.902720463108541 L254B-F465B 0.90186789652119 V320B-I415B 0.896876652272861 V320A-I415A 0.895537991560727 V320C-I415C 0.894108452126978 I196C-I375C 0.879854179644566 C252A-G503A 0.877873825268171 C252C-G503C 0.876708903400377 C252B-G503B 0.875389777132965 I196B-I375B 0.874294690935315 I196A-I375A 0.861501406942237 L506A-L518A 0.860200619821044 L506B-L518B 0.855994729229444 L506C-L518C 0.855525811169432 W101A-L330C 0.845134397775572 W101B-L333A 0.843597154207418 L330B-W101C 0.84188822860701 L312A-L460A 0.835522954728017 L312B-L460B 0.834503382775805 L312C-L460C 0.834342811748548 I78C-L508C 0.810512907558719 I78B-L508B 0.810476107414275 I78A-L508A 0.810434228725263 I196C-W374C 0.806529900256497 I196A-W374A 0.802116081568083 W101B-L330A 0.799004356860876 I196B-W374B 0.798765716430356 W101A-L333C 0.788026308646993 D470C-A493C 0.784425514868147 F110B-I196B 0.783055041293281 W189A-A406A 0.781299186699972 W189B-A406B 0.779811507948646 F110C-I196C 0.778986337630521 L333B-W101C 0.778178122450722 T287A-F465A 0.777583165580778 D470A-A493A 0.777409078376209 W189C-A406C 0.776501529213296 T287B-F465B 0.772547744804509 T287C-F465C 0.770949905887761 D470B-A493B 0.768657082290672 Q260B-L461B 0.768333479452249 F110A-I196A 0.764131211334005 I261C-L461C 0.763715689492572 F248A-T499A 0.761722802038188 I261B-L461B 0.760560555605064 F248B-T499B 0.760006407133794 I261A-L461A 0.759692894756194 W194C-L402C 0.758018826737808 L507B-L518B 0.755953890268299 F248C-T499C 0.755299668241612 L507C-L518C 0.754475162049613 L507A-L518A 0.7534435774059 W194B-L402B 0.746753107868672 W194A-L402A 0.745458865292148 Q260A-L461A 0.739408437252141 S183A-I337A 0.736992747999382 H188C-P193C 0.731455776121458 S183B-I337B 0.731108483817579 H188A-P193A 0.731054417964354 H188B-P193B 0.73069976239247 Q260C-L461C 0.73020748058007 S183C-I337C 0.728549254808454 I104C-T351C 0.727477540478537 I104B-T351B 0.721917524184532 L332B-W101C 0.72117259182021 A245C-W377C 0.720673122488848 A245B-W377B 0.719810555139859 A245A-W377A 0.719533521924042 I104A-T351A 0.718401723993517 V180C-N339C 0.718022807115743 V180B-N339B 0.716770937206679 V180A-N339A 0.716279890191754 L191C-L402C 0.70131017231242 F321C-I415C 0.699584128886848 A317C-W412C 0.698729826319194 A317A-W412A 0.698536882047409 W373A-A526A 0.695134599107806 G349B-I337A 0.695098048237254 W373B-A526B 0.694598025785128 A317B-W412B 0.694066426249527 W373C-A526C 0.694017431408286 L288A-V497A 0.691763402297035 L288B-V497B 0.691667945173289 L191B-L402B 0.689752519612343 F345B-L341A 0.688783246958805 L288C-V497C 0.688756280536739 F345A-L341C 0.688631022106694 F321B-I415B 0.688299947287458 L341B-F345C 0.688035925322307 L191A-L402A 0.688002396106602 F321A-I415A 0.685465820330286 F248A-A525A 0.683008771408738 F248B-A525B 0.681331969268001 F248C-A525C 0.679840500332865 L288A-A500A 0.675691699790707 L288B-A500B 0.674458314163606 L312B-I456B 0.671348085075836 I375C-F529C 0.670528369609841 L288C-A500C 0.670017966600562 I375B-F529B 0.669912178575225 I375A-F529A 0.668965308001164 F248B-I502B 0.667647605251026 F248A-I502A 0.667499236281919 F248C-I502C 0.667393820425409 L312A-I456A 0.663845154889691 L312C-I456C 0.658152438076554 I337A-T410A 0.654605572129248 I337B-T410B 0.654547902332682 A245C-F529C 0.654144656127093 F321B-I329B 0.650908313247288 L316A-A457A 0.650904501996599 L92A-V524A 0.65007529247586 F321C-I329C 0.649894060222787 L316C-A457C 0.64982671786969 F321A-I329A 0.649481953202685 L316B-A457B 0.649436462167307 A245B-F529B 0.648165726807947 I337B-G349C 0.646820411666861 N339B-A348C 0.646237712368541 V247B-T499B 0.644673069241719 T73C-I502C 0.643366092677812 G179A-S336A 0.642648253566244 L92C-V524C 0.640347147041771 G107A-W371A 0.639445767081867 G179C-S336C 0.639205938196043 I337C-T410C 0.63902159753041 T73B-I502B 0.638546189219003 V247A-T499A 0.638419771172966 G179B-S336B 0.635309579254077 T73A-I502A 0.634864274146015 A245A-F529A 0.634748967408072 G107B-W371B 0.634151800051625 G107C-W371C 0.632786805929468 V247C-A472C 0.631988071740703 L92B-V524B 0.631315702524088 I244A-A525A 0.63105309455064 V247B-A472B 0.626326166347718 V247C-T499C 0.626324659113425 V247A-A472A 0.625118549788107 I244B-A525B 0.621653612988906 G349A-I337C 0.621542962277998 L333A-V411A 0.615476420692 I244C-A525C 0.614466877860515 A348A-N339C 0.611883236053781 L333B-V411B 0.610164930385915 S336B-G349C 0.606222880114119 F292C-A493C 0.605729959772064 F380A-A472A 0.605680857095765 L241C-P528C 0.605609955922901 F292B-A493B 0.60535500318807 F292A-A493A 0.605243057756248 F380C-A472C 0.604666626522376 F380B-A472B 0.603845899207481 A348B-N339A 0.601248613780314 L241B-P528B 0.600726621652259 L312A-A457A 0.600482625686131 L312B-A457B 0.599829762682327 L254A-A500A 0.593669777082689 L254B-A500B 0.59113672030067 L312C-A457C 0.590547844079822 L241A-P528A 0.589293849341478 N309C-F464C 0.589120449380601 L254C-A500C 0.588965623861145 L333C-V411C 0.586659137409116 N309B-F464B 0.58501392331017 N309A-F464A 0.578977131342551 F103A-I368A 0.578079896113541 R350A-G338C 0.576826840779439 G262C-V283C 0.575021946582924 I104A-A348A 0.574845394129866 V70C-I244C 0.563646945360973 V70C-I502C 0.563194457596774 V70A-I244A 0.562988602717789 V70B-I244B 0.562592369175889 A313C-L460C 0.560607444451499 G262A-V283A 0.560575770235548 I104B-A348B 0.560001443911244 V96C-I368C 0.559753320450139 F103B-I368B 0.559465369674461 I329C-T419C 0.559135117689154 G262B-V283B 0.558748845093583 V70B-I502B 0.558068967329738 S354B-G179A 0.55752382383297 G349A-S336C 0.55727732203655 V96A-I368A 0.556720571363282 G100B-I368B 0.556072560320755 V96B-I368B 0.555743125552912 V70A-I502A 0.555596604825582 A317B-F416B 0.553558567918121 A313B-L460B 0.553061455252561 A313A-L460A 0.552138719117957 A317A-F416A 0.552090135990756 I104C-A348C 0.550835764865234 G179C-N339C 0.550528667593241 R350B-G338A 0.550271678933869 A352C-L363C 0.549513058829618 G338B-R350C 0.549340097132132 G179A-N339A 0.549136682136959 I329B-T419B 0.54795890756815 F103C-I368C 0.547806202114933 G100A-I368A 0.547357914449243 F187A-F344A 0.547139702589975 A317C-F416C 0.547046555517298 G179B-N339B 0.545465375589427 I329A-T419A 0.545080712688588 F187B-F344B 0.544869023761617 S342B-F345C 0.543309574889994 A352B-L363B 0.540709175292545 G100C-I368C 0.540610326649462 V327B-D97C 0.540583734924507 F187C-F344C 0.540539825682669 S354A-G179C 0.540130182495885 A352A-L363A 0.539921829467898 F465B-D470B 0.536402435498972 F465A-D470A 0.536370752357584 G179B-S354C 0.534767980500578 F465C-D470C 0.533169884613089 F103A-L530A 0.531058437536857 L92A-N520A 0.531020962750265 F103C-L530C 0.530612440056847 L92B-N520B 0.53004774431097 F103B-L530B 0.529550130296074 L92C-N520C 0.529235099150408 P334B-A348C 0.528211467348714 D97A-V327C 0.524413562982808 F248A-T522A 0.523125882277919 L507C-N517C 0.5197073868592 L507A-N517A 0.51963289604278 L507B-N517B 0.5192337538955 F248B-T522B 0.518704423580608 L507C-L515C 0.513909791639828 A242A-F529A 0.512506683334336 F248A-S376A 0.511536044995383 A66C-I243C 0.510439147482597 F103A-A372A 0.510432568064489 C252A-L518A 0.509867436679965 F248B-S376B 0.509260024933579 F103C-A372C 0.509197419375856 A66A-I243A 0.509146200677796 F103B-A372B 0.508618940971695 C252B-L518B 0.508414759224875 F248C-S376C 0.508271793157043 A242B-F529B 0.507789681608332 C252C-L518C 0.507391845304439 G349B-S336A 0.506778062062375 F345B-S342A 0.505620556571214 F248C-T522C 0.505054517748928 A242C-F529C 0.504897455786262 A469C-T499C 0.504745999540619 A469B-T499B 0.504590921359528 A469A-T499A 0.504401654135597 L363C-F369C 0.502902177339403 L363B-F369B 0.502649508774239 L363A-F369A 0.502632244650693 D97B-A352B 0.502393298653107 A66B-I243B 0.502360701721486 F292C-D470C 0.501222271311591 I295B-A493B 0.500218638093331 F345A-S342C 0.50010600407389 F292A-D470A 0.498326118250287 F292B-D470B 0.497694730114744 W194C-A406C 0.495576686184386 W194B-A406B 0.493931392945661 A348A-P334C 0.493633175987519 W194A-A406A 0.493517220237583 D97C-A352C 0.493036107616836 S336C-S414C 0.490307965211305 D97A-A352A 0.490015447172136 S284C-L504C 0.488566091519337 S336A-S414A 0.485459917834876 S336B-S414B 0.484582527134173 S284B-L504B 0.483948981709513 F248A-V521A 0.483234282517275 S284A-L504A 0.482730812311822 V247C-W495C 0.48222450054612 V111C-L191C 0.481109714355935 T246C-A472C 0.481025472777008 T246B-A472B 0.480375427218474 F248B-V521B 0.479410640948426 T246A-A472A 0.478917415393114 F248C-V521C 0.473224333787227 V247B-W495B 0.47294062698053 C252B-W373B 0.472030480579438 C252C-W373C 0.471758763311284 V247A-W495A 0.471175022642983 V111B-L191B 0.467615417752441 L241C-F529C 0.46570775630687 L288C-I466C 0.465074593010528 L288B-I466B 0.464950996564876 L288A-I466A 0.463822787796771 V327B-W101C 0.462367197532586 A348B-P334A 0.46141420206367 A372C-V524C 0.460418024671606 V111A-L191A 0.460180990394788 F110C-H192C 0.457439933011711 T246C-F380C 0.45688941310431 A372B-V524B 0.456562408363986 L330C-S414C 0.455834618749596 A372A-V524A 0.455635905633331 L254C-S468C 0.455268419157967 L330A-S414A 0.454205551277885 L254A-S468A 0.454190548241552 F110B-H192B 0.453823868176883 L254B-S468B 0.453494856465189 L330B-S414B 0.453118466618684 F110A-H192A 0.452396468457829 W101C-F106C 0.451991127165911 I261A-G462A 0.451094944758095 I261B-G462B 0.450997648863871 I261C-G462C 0.450974859353167 T246B-F380B 0.44934725985259 C252A-W373A 0.449011083394284 I291A-G496A 0.445444286422958 T190B-A406B 0.444439706530673 I291B-G496B 0.444073734021521 L241B-F529B 0.442886915749539 T246A-F380A 0.44287899843466 F187A-H192A 0.442485745499468 T190A-A406A 0.442447723022161 H188C-T367C 0.442416274253193 I291C-G496C 0.441812179045534 W101B-F106B 0.441467597384934 H188B-T367B 0.439306907206682 H188A-T367A 0.438535153158144 T190C-A406C 0.435247462611181 I104A-G349A 0.432748496748744 F187B-H192B 0.43256017490281 W101A-L332C 0.432489002434444 S376A-I523A 0.432167283519818 A242B-P379B 0.429995862014065 W101A-F106A 0.429494788518651 A242A-P379A 0.429334409763912 G249A-W377A 0.424459768664702 A242C-P379C 0.424250016878672 V247A-A498A 0.423448795324077 V247B-A498B 0.422711922337771 L241A-F529A 0.422242588485833 V247C-A498C 0.421792926213519 V180A-N343A 0.420637350203375 V180C-N343C 0.420195148933549 W373A-T522A 0.419128324978443 G249B-W377B 0.41912724746796 I104C-G349C 0.418770870209724 P65A-I240A 0.417923899872972 V180B-N343B 0.41764191888612 P65B-I240B 0.416736364185404 F187C-H192C 0.416416281699621 F369C-T522C 0.415825221628921 P65C-I240C 0.415666546305878 W373B-T522B 0.414235513635411 W373C-T522C 0.408022895299203 V280A-L504A 0.407854592535134 V280B-L504B 0.407737810560344 V280C-L504C 0.407732218506255 I104B-G349B 0.407307222653856 S376B-I523B 0.407152314968293 S306B-T467B 0.398644703545579 S306A-T467A 0.398621365368106 V74B-I502B 0.398313967283631 V74A-I502A 0.398183694507976 V74C-I502C 0.398163010158393 L254A-A469A 0.397637271038807 G249C-W377C 0.397363689796858 S306C-T467C 0.396953311494608 L254C-A469C 0.396614607177833 F369B-T522B 0.395088849830824 L254B-A469B 0.39331363516032 W189A-S409A 0.393055805069007 A66C-I240C 0.392962247604046 A66B-I240B 0.39286561036488 I375B-A526B 0.392863741928838 A66A-I240A 0.392399310832146 I375C-A526C 0.392150450602858 W189B-S409B 0.391468154289198 S336A-T410A 0.391328520327252 S336C-T410C 0.390782106406643 V70A-A498A 0.390036691464037 S336B-T410B 0.390027974769819 V70B-A498B 0.389707168404292 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