[Thu Aug 25 00:48:01 2016] >Input file: TM2XOWATG.pdb >TM segments: A96-112_A138-159_A172-186_A198-210_A230-246_A253-267 MAIDEN score = -65.7884 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C104A-L143A 1.15946217201606 W241A-I255A 1.1290553366767 W158A-V204A 0.992280454277501 W157A-V204A 0.9676529435584 L143A-L200A 0.854200008331233 W158A-L207A 0.809138360725068 F139A-L183A 0.773406234792362 S185A-I255A 0.729527069870636 Y205A-I237A 0.680375898093031 A103A-L143A 0.666659561387188 V238A-D243A 0.65837903834294 A206A-L262A 0.649380837216786 V204A-G257A 0.632857642949729 M144A-L200A 0.625730649239405 A239A-F245A 0.618423387496573 H145A-L200A 0.614612355159167 F139A-G186A 0.609505094379811 A142A-V203A 0.589617765852193 D243A-H254A 0.569626108175171 V96A-I175A 0.55854248843363 H145A-H150A 0.550460995550569 Y205A-G261A 0.545702997611985 F108A-M144A 0.533611804808031 S181A-G202A 0.531180121518517 Y210A-A263A 0.524317557860819 V107A-L143A 0.523957118579908 Y210A-L258A 0.508061818107549 G240A-F245A 0.485485290490226 Y205A-I255A 0.482537210464005 M208A-A233A 0.468474327717743 Y138A-G186A 0.466561761246019 M208A-L258A 0.460731443022159 M101A-L155A 0.460262811354728 F108A-I151A 0.443303853246715 F139A-L179A 0.439339825720828 Y138A-A182A 0.432357880709498 G202A-I255A 0.429728376470875 I177A-L207A 0.424017365347237 A206A-L258A 0.422219985510279 I151A-G199A 0.417563121323882 M208A-I237A 0.403145952427924 V238A-L244A 0.397833620867126 T97A-W159A 0.393794275674986 N154A-W159A 0.384774192101141 A239A-L244A 0.384592574589871 M144A-G198A 0.38236939954817 S181A-G261A 0.378614101659485 H141A-S181A 0.378189394678828 F146A-I151A 0.374760302404912 C104A-G198A 0.368857615495285 V203A-L258A 0.364913604296391 A206A-A259A 0.364823667034682 L184A-G257A 0.36437663509799 Y138A-G202A 0.360801091178768 L184A-A256A 0.354888008329096 A142A-L200A 0.352604131336419 M101A-H145A 0.352472561512188 Y210A-V260A 0.351842917155686 W158A-V203A 0.349759790989902 S201A-A253A 0.342701800133126 A142A-S201A 0.341071227829204 V96A-L174A 0.332152585358081 Y138A-V203A 0.3244044247872 S181A-A256A 0.32330577287817 V176A-M208A 0.32146946993357 Y210A-M264A 0.320654174909496 M208A-G261A 0.31984000952567 F108A-L143A 0.319117881918887 G240A-H254A 0.318247031163415 L184A-V260A 0.317682680734045 V204A-L258A 0.314437973641393 A182A-L200A 0.313973288921978 I177A-G261A 0.31299703335388 G202A-L258A 0.312150031879786 G199A-G240A 0.308563547234447 I180A-V260A 0.307358221261987 A142A-G257A 0.307072000479116 K173A-A206A 0.306085604812218 C104A-V204A 0.303898746617147 M100A-L143A 0.301365908375435 F146A-G198A 0.30115178660326 L207A-L258A 0.296935463420986 M149A-W236A 0.296831780420224 H145A-I151A 0.296137810153899 Q112A-M144A 0.295229933344904 W241A-H254A 0.294866507726824 M149A-D243A 0.294088801396291 L184A-A253A 0.292694789845378 I177A-V203A 0.291084996851298 C104A-I151A 0.290748913127889 M144A-G199A 0.28708652900494 H145A-H254A 0.28559975398943 S185A-V203A 0.285548887719829 W236A-W241A 0.284931042482071 L179A-G261A 0.284167957345992 I180A-M264A 0.279284817646646 N154A-G198A 0.278255529094051 M144A-I151A 0.268607889181112 V107A-M144A 0.267412307924296 S181A-G209A 0.26470778800883 Y205A-L258A 0.253779817672124 W157A-G202A 0.252281033463054 G186A-Y205A 0.251945128264262 A206A-A233A 0.2516236856056 I177A-M264A 0.248815277272386 L143A-H150A 0.247191352899728 L143A-T178A 0.239420349867062 L207A-H254A 0.238523587310352 S147A-G198A 0.23614586687685 I151A-V204A 0.232881434344814 W158A-G199A 0.224742499494524 S181A-V203A 0.219796428610668 G186A-A253A 0.219554728403504 H141A-T178A 0.216399758057672 M100A-V176A 0.214303024517917 V105A-L155A 0.20994373316027 W98A-W159A 0.207435804152474 V106A-I151A 0.206767040719545 S147A-G246A 0.205797062662833 F108A-F146A 0.205642953666063 T97A-L174A 0.205352374248067 V96A-I177A 0.204321553581416 L183A-V203A 0.204318676708204 G209A-V260A 0.204214922044944 G209A-W236A 0.20259767311674 H141A-L179A 0.199110503376192 G198A-V203A 0.198999040501419 F153A-A233A 0.198034660114137 M149A-F245A 0.196771733413516 A110A-F146A 0.196538647245837 L174A-G261A 0.196216701246353 L143A-G198A 0.196025855626258 T178A-G209A 0.19166748671962 C104A-W157A 0.190737927639055 G209A-A265A 0.189104777057697 Y138A-G199A 0.188420697581478 F108A-H145A 0.184980050900502 N154A-S185A 0.184926759225663 V107A-H145A 0.184753757182818 V107A-G198A 0.181346156987599 G209A-H254A 0.179414239678662 M208A-G240A 0.177950117456358 V105A-M149A 0.177567074029331 N154A-G240A 0.176600451018101 I151A-L200A 0.176192475907021 S181A-A265A 0.174906377486228 L148A-G246A 0.174880483851397 C104A-L148A 0.174731430756977 F139A-H254A 0.171287779876711 I180A-A259A 0.170560155145095 S185A-M208A 0.17052024906552 L143A-S181A 0.168813410242328 W159A-T178A 0.168548317386294 W241A-G246A 0.16846918843852 T140A-I175A 0.16820257049943 H141A-A253A 0.166325050679222 V176A-A263A 0.16608335500136 T178A-A256A 0.163485028679108 N154A-V204A 0.162351764289534 V106A-L143A 0.162039307167985 L143A-G199A 0.161953636649035 G172A-A265A 0.161636831452177 F108A-A142A 0.161141913442622 M111A-H145A 0.160975538730994 A142A-G202A 0.160064109389054 N154A-F232A 0.159653481784506 S181A-G199A 0.159385277145514 T140A-L183A 0.158511721534218 L155A-L200A 0.157234332854671 V106A-A142A 0.155280504760682 M100A-S181A 0.154101333641622 T178A-L200A 0.15237715829374 M100A-G172A 0.152240321004547 H141A-G199A 0.150625964944131 I102A-A142A 0.150222694651126 V176A-V267A 0.149570777363709 A110A-G199A 0.148872812404033 F108A-F153A 0.147350763789219 H150A-A239A 0.146960945451648 I102A-T140A 0.146791080857138 W158A-K173A 0.144670229934301 M101A-A142A 0.144471635812524 M208A-V238A 0.144014867895792 T178A-G199A 0.143870187646931 S147A-N154A 0.141849233053621 L200A-G240A 0.141849058121247 V107A-G199A 0.141518322437912 M149A-G199A 0.141489320790415 I109A-I151A 0.140273325868668 Y210A-I237A 0.140254013998069 L179A-S201A 0.139425497660836 Y210A-L234A 0.138156764361573 I175A-Y210A 0.137143094791145 V176A-V204A 0.137125441271383 Y205A-I231A 0.136611229590469 M144A-S185A 0.135832290780666 M101A-L174A 0.135643366758809 K173A-S181A 0.135363918694881 W236A-L244A 0.134703544009737 V99A-V203A 0.13439632411492 I180A-S185A 0.133663676388496 T178A-A253A 0.13313670709189 A142A-A256A 0.132352005120579 S185A-L207A 0.132165842686883 A239A-A253A 0.132103563422621 M101A-V203A 0.13202706931259 V99A-V176A 0.13013522561981 L184A-L207A 0.129458489487686 H254A-G261A 0.128138020601558 L174A-V204A 0.127617494101665 V176A-L207A 0.126858396580292 K173A-V203A 0.126689478513889 T178A-A259A 0.126509154902209 K173A-G261A 0.12618253907862 L179A-Y205A 0.125927927174438 G240A-G257A 0.125504172270391 Y205A-A265A 0.125438235784503 I237A-G261A 0.124464465095893 K173A-V267A 0.124351907077838 G209A-I235A 0.124338712312559 S185A-M264A 0.124257196118116 H141A-A256A 0.122811485093171 V106A-H145A 0.122152224077523 L174A-L200A 0.12169967306991 V105A-S147A 0.121399788701522 H150A-W236A 0.120819512700477 L183A-Y205A 0.120723893162833 L174A-Y205A 0.120630895365955 L143A-L148A 0.120454046498377 H141A-V203A 0.120178333503997 I151A-W159A 0.119960194200132 A103A-N154A 0.119421589058767 F146A-G246A 0.119167272255034 M111A-G199A 0.118899280763507 T178A-A265A 0.118632031659519 G199A-G257A 0.118510453519125 V99A-L143A 0.118450017153188 G172A-Y210A 0.118025830488111 L184A-L262A 0.117573664420747 A142A-I177A 0.117030608129649 A103A-S201A 0.116725968606457 A206A-G240A 0.115900514233973 G186A-A259A 0.115895647748572 C104A-Q112A 0.115798551488167 L179A-L258A 0.115686193188777 W158A-W236A 0.11494902077708 V203A-V260A 0.114575747501373 Y138A-A253A 0.114385449430002 L148A-L200A 0.114191694963459 T140A-G202A 0.113622576787184 L234A-L244A 0.113599378228267 G186A-V204A 0.1135843831186 V99A-G172A 0.113140604259449 V238A-G246A 0.112913373184872 W158A-G202A 0.112361241618632 A233A-G261A 0.112098711407445 V203A-G240A 0.112000988279813 A239A-G257A 0.111473444074626 T140A-S201A 0.110590191232156 V176A-G257A 0.110585699678496 H254A-A263A 0.110512877648072 A103A-L200A 0.110512156953029 Y210A-A256A 0.110421672840994 T140A-V203A 0.110297218175707 H141A-L184A 0.110036342188003 V204A-G261A 0.109646434494587 W98A-L143A 0.10955952337827 G209A-V238A 0.10917039342232 V238A-A259A 0.108732655154891 Q112A-F146A 0.108471327558883 H150A-G199A 0.108443291062339 A206A-V238A 0.108122993346198 G198A-G257A 0.107610105238656 G209A-A256A 0.10710211477856 Y138A-L200A 0.106736089258735 I177A-A206A 0.106726832665682 G199A-A206A 0.106703837919312 Y138A-S201A 0.105950239013202 G186A-L258A 0.10583670262246 Y210A-A233A 0.105561910992844 S201A-L207A 0.105127854193421 H141A-S201A 0.10484687534741 V204A-A263A 0.104086181530678 V106A-L155A 0.103482386982566 I177A-G209A 0.102377241726476 V107A-L152A 0.101725772236956 V204A-G240A 0.101506687874544 V106A-L148A 0.101464294391516 H150A-W158A 0.101321668800978 A142A-A182A 0.100055158501492 I151A-S201A 0.0998058646793424 L155A-M208A 0.0996761756743325 G202A-W236A 0.0994792999742735 G186A-A206A 0.0992730329627876 L234A-L262A 0.0992051999428973 C104A-V203A 0.09860176014719 Y205A-A239A 0.0981960618976139 M100A-L152A 0.0980547155046456 G202A-A233A 0.0977984620712604 L155A-A182A 0.0975527964294809 V106A-L152A 0.0975168164837501 A110A-L143A 0.0974459448370943 L183A-A259A 0.0974063249675776 V105A-H145A 0.0973004430286863 S201A-V260A 0.0971650016277552 S185A-H254A 0.0970614618014909 N154A-L200A 0.0970482360723253 M101A-W159A 0.0968669625041549 L184A-V204A 0.0966411947961174 G257A-A265A 0.0965227726117045 H141A-G186A 0.0964330846206281 T178A-G186A 0.0960796282222434 G202A-G209A 0.0959293804266252 V107A-H150A 0.0956446902560799 G198A-H254A 0.0948501797793266 A110A-S147A 0.0948120465461528 I180A-A263A 0.0940055444819095 S185A-A263A 0.0938709374462192 L156A-I230A 0.0938017842234385 I102A-L148A 0.0937820500231044 L179A-L207A 0.0937232195456237 A103A-H150A 0.0932918184664304 Y138A-L143A 0.0927741923162149 L244A-I255A 0.0906409094770054 T97A-G172A 0.0906127389161255 V96A-V203A 0.0901519129771353 V176A-A206A 0.0899155125217944 I151A-V203A 0.0898996547643301 Y210A-I255A 0.0895945722679192 L200A-A256A 0.0892749585569619 V99A-L200A 0.0892567724551185 S201A-A259A 0.0891309003217406 F153A-W158A 0.0887607550188667 V99A-L155A 0.0880268912079272 N154A-A206A 0.0880198711383171 L207A-I235A 0.0875339882108344 W241A-L258A 0.0875139571988957 V238A-A256A 0.0872788657151973 Y205A-V238A 0.0869775753151095 T97A-K173A 0.0864726242167433 M149A-L244A 0.0860834467780818 A142A-A206A 0.0860537355382918 M208A-I255A 0.0859982240616644 M208A-I235A 0.0857566401387588 T140A-H145A 0.085574306442602 S201A-W241A 0.0854647776703872 A182A-G261A 0.0853389103382574 A103A-V203A 0.0849969629410028 M100A-I180A 0.0845190925486041 V105A-G198A 0.0841898143257028 W241A-A256A 0.0838091035202972 N154A-A182A 0.0838010520677628 A142A-L174A 0.0835600186498038 G199A-G246A 0.0831218423016211 V176A-G202A 0.0822900279409839 S201A-G261A 0.082195734537402 L200A-I237A 0.081411149979381 V107A-L155A 0.0807016561832532 A142A-I175A 0.0805081490236823 L143A-G186A 0.0801560341308885 G198A-G240A 0.0800023201043161 A182A-L207A 0.0799446176657899 L148A-G199A 0.0796897225890714 A233A-I255A 0.0796356374377325 W158A-A233A 0.0795864877736352 A206A-I235A 0.0789647156610662 M100A-A182A 0.0785678357870354 L207A-F232A 0.078533819047341 A142A-I255A 0.0781795438659061 I109A-A142A 0.0781721627278458 H145A-V203A 0.078159637430697 N154A-I237A 0.0779675886060578 T178A-A263A 0.0775903601104853 I175A-G261A 0.0775819360317964 A142A-W158A 0.0774995883621914 A142A-L155A 0.0771741873373109 L155A-S201A 0.0771566220363304 M101A-T178A 0.0763569926447392 L183A-V204A 0.0759863984730038 W158A-I175A 0.0758679921176224 M144A-V203A 0.0756363997083103 A142A-L184A 0.0755247160186796 V99A-I151A 0.0752646000968795 W159A-S201A 0.0752491384441168 G186A-G261A 0.0750826612024887 L174A-V260A 0.0745406565529799 W157A-I237A 0.0741663248316888 A142A-L258A 0.0739870982617745 T178A-M208A 0.0739210535218591 F139A-L184A 0.0737984499198361 L143A-F153A 0.0736044157573466 I180A-G261A 0.0728858606082738 M101A-I151A 0.0716065439640783 F108A-G198A 0.0710958115012534 M111A-H141A 0.0710134972680869 G240A-A256A 0.0709705220944787 G199A-F245A 0.0708061362425125 L207A-I231A 0.0700852952682625 L143A-L179A 0.0698826137345474 Q112A-I151A 0.0698693832501738 T97A-V204A 0.0689955960839281 S201A-A233A 0.0688596764225036 A206A-F232A 0.0686901735537129 N154A-A239A 0.0684720832054433 L183A-G198A 0.0681118921542128 N154A-G257A 0.0679054935742798 T140A-V204A 0.0672560524790287 M208A-A256A 0.0671770029306273 T97A-C104A 0.0668003183060222 H141A-G257A 0.0665510271691074 V204A-A256A 0.0663327252818147 L143A-A253A 0.0662786293265871 L179A-A253A 0.0662218006258348 A233A-H254A 0.0660086930341124 A110A-L148A 0.0659690997675807 A233A-L262A 0.0658540356537276 M144A-M149A 0.0657038717848462 V203A-A233A 0.0655145230066836 T140A-L184A 0.0654264302238386 A103A-M111A 0.065258356435995 V107A-L148A 0.0651552965471325 L183A-L258A 0.0650188004792693 G202A-A256A 0.064993576858118 H150A-G202A 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