[Wed Aug 24 17:15:05 2016] >Input file: TM2XTVATD.pdb >TM segments: A148-166_A170-185_A199-213_A229-242_A244-267 MAIDEN score = -53.8173 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- W158A-V204A 1.0279004365809 W157A-V204A 0.934642091452043 L161A-L207A 0.887720747772744 W158A-L207A 0.787655821097035 L213A-L262A 0.75885087377743 Y205A-I255A 0.749357181177835 V165A-L174A 0.719443126912616 M149A-F245A 0.715152647867238 A206A-L262A 0.662690449687437 S181A-G202A 0.647315754386223 W158A-G163A 0.64523507916397 I177A-G261A 0.612082789387477 A239A-F245A 0.610726973662871 L161A-V211A 0.59802231412732 M208A-L258A 0.581981625093545 V165A-K173A 0.571712267756512 A206A-L258A 0.56464008303133 W159A-A164A 0.552853008982802 Y210A-L258A 0.547815030587497 E166A-S171A 0.531875161371052 Y210A-A263A 0.504030212935186 I177A-L207A 0.479978687199631 S185A-V203A 0.470944058549583 L184A-A256A 0.470501261234611 V165A-S171A 0.468017516529867 T201A-H254A 0.467424076432779 S185A-I255A 0.457900710639613 I151A-G199A 0.452724409197317 E166A-T178A 0.431478505357863 G202A-I255A 0.422206115645317 I177A-M264A 0.409913321680639 Y210A-V260A 0.407643918311613 L213A-F266A 0.398316501586478 H150A-S248A 0.396818694900307 V203A-H254A 0.395855715627498 N154A-W159A 0.38719897454569 V211A-A265A 0.386150246970935 I177A-A206A 0.384032040712937 A164A-V211A 0.377391636401473 L161A-V204A 0.374420771470839 I180A-V260A 0.371487109649516 M208A-I230A 0.367407008580032 L161A-M208A 0.360274275372084 S248A-H254A 0.349355122147624 Y205A-G261A 0.347404270715582 M208A-G261A 0.344480723878329 W241A-A250A 0.342014826393553 S181A-A256A 0.341918948445507 V203A-L258A 0.341796259538704 L184A-A253A 0.323415095901147 Y210A-M264A 0.323237652624378 V204A-G257A 0.315707238135341 G199A-A253A 0.315525953209086 A206A-A259A 0.313428395902419 A182A-L200A 0.310867419870007 S185A-A253A 0.310770990937716 G163A-Y210A 0.310159192671813 W157A-G162A 0.309115946004228 G199A-G257A 0.301663754199551 I177A-V260A 0.29884409496846 L207A-L258A 0.297954515187116 N154A-V204A 0.294698222976477 I177A-V203A 0.287706030885366 S185A-G261A 0.286140844835644 Y205A-L258A 0.285502061397475 L184A-G261A 0.279378976455826 S181A-G209A 0.277372829242495 L179A-G261A 0.273685995077991 V203A-V260A 0.271154554552608 S181A-V260A 0.263563139455498 G202A-L258A 0.263260775994359 V204A-L258A 0.262940689741194 L184A-G257A 0.259299590791259 W159A-T178A 0.258589061301428 W236A-W241A 0.256595646120371 W158A-G199A 0.254016073125159 G162A-L200A 0.251791647202473 G202A-A253A 0.25121638916163 V176A-M208A 0.250821574715232 L207A-W212A 0.248029409699232 L207A-H254A 0.247292280689188 W157A-G202A 0.246774174288984 N154A-G240A 0.245146296657245 L156A-T201A 0.24214420391647 L161A-T178A 0.241754371103551 M208A-A233A 0.24109331406635 F153A-G199A 0.23336317472253 L152A-G199A 0.232776551927228 N154A-G163A 0.229393954831274 W158A-V203A 0.227817536803636 Y205A-F232A 0.226819613704461 F245A-A250A 0.22393944173304 L152A-T201A 0.217414331307847 S185A-S248A 0.215497181812381 A182A-G202A 0.21508939617334 G162A-V176A 0.21503280505709 T201A-W241A 0.214541670624276 V203A-M264A 0.214213470003686 A182A-G261A 0.212309791643547 M149A-V238A 0.209173386431614 L183A-V203A 0.208843319984127 H150A-F245A 0.208743264138349 G162A-T201A 0.206375554318169 V165A-V211A 0.204953270751231 I180A-T201A 0.203489318099743 I151A-V204A 0.202362853215359 L184A-M264A 0.202178767046136 W157A-I230A 0.200146310702132 L161A-G261A 0.197555227389083 S185A-L258A 0.195168997056275 Y160A-V203A 0.190464103356584 G170A-T178A 0.189365971681642 G163A-G172A 0.188304132399389 T178A-A259A 0.186630772474539 W158A-K173A 0.184528957891767 T178A-A206A 0.18441350188035 G209A-G252A 0.18376350079809 T201A-G261A 0.183104734643689 G162A-L207A 0.182293976173568 Y160A-Y205A 0.181609061309602 W158A-L200A 0.179706022802453 G202A-I237A 0.178982325125373 G162A-I177A 0.17867198283736 S185A-M208A 0.178242858415561 Y160A-G209A 0.17751709337094 T178A-G209A 0.176318838625398 E166A-Y210A 0.175337876204976 L183A-G261A 0.174653797901699 E166A-V211A 0.172572991634802 Y205A-W212A 0.172493698331964 T178A-A256A 0.17209595333757 G199A-H254A 0.171557086030003 H150A-F232A 0.171315018238517 G202A-L262A 0.170915931204433 G209A-A265A 0.169467515871439 G202A-A256A 0.169303519956462 G240A-A256A 0.167430869343412 V238A-M249A 0.167318738572691 I180A-G261A 0.167196499519235 A239A-L244A 0.165154953858392 A206A-G240A 0.164836897808196 T201A-G209A 0.164669572816388 F153A-G240A 0.164170064020102 G162A-L174A 0.163844302847187 W158A-G202A 0.163445100628592 H150A-W241A 0.162650341807953 A239A-G252A 0.162457653723552 W158A-L229A 0.162012067487056 A233A-G257A 0.160672027589228 G202A-G240A 0.160253099228872 G209A-L229A 0.157896212784341 M149A-N154A 0.15717418283008 H150A-Y205A 0.157154193075447 I151A-L200A 0.157111174257273 T178A-L200A 0.156754255224717 G199A-G240A 0.156353998539827 W158A-W212A 0.155207773551463 G162A-G172A 0.154461827488859 L200A-G240A 0.154221057208139 I177A-T201A 0.154201764558984 M208A-N251A 0.15311248934858 M149A-I235A 0.152987156507921 Y210A-H254A 0.15278759500899 V211A-G261A 0.152236277857144 H150A-T201A 0.152139137474587 L244A-N251A 0.152071013206879 W159A-L229A 0.151757287761256 A233A-G261A 0.151458316944182 N154A-L200A 0.149852772554262 S181A-L258A 0.149323005378285 I177A-A259A 0.147763007561155 G199A-A250A 0.147163989023718 V176A-A263A 0.146698229096958 G162A-G202A 0.143815206133612 L161A-W212A 0.14313871969667 L179A-Y205A 0.142143508021762 F153A-W236A 0.141781758312518 I237A-A253A 0.141679064281099 G172A-M264A 0.141430465725596 T178A-L258A 0.140170424505893 A239A-H254A 0.140025848329187 L234A-I255A 0.139918800826659 G162A-I175A 0.13858391688209 G209A-H254A 0.137922638072625 W241A-L258A 0.137175453080912 L161A-G170A 0.13697969708485 V238A-S248A 0.136768915073749 H150A-V203A 0.136589758446377 L156A-I230A 0.135598825513621 T201A-F245A 0.13540516902402 W158A-W236A 0.135110820469373 A250A-G257A 0.134400541762695 A164A-A206A 0.133496586676169 S181A-G199A 0.133162294582737 I177A-L213A 0.133129981272708 A164A-V204A 0.132481236491164 L155A-W236A 0.130582434437649 G199A-G252A 0.128493632987792 S181A-A263A 0.128139873683626 Y205A-A265A 0.12782376006965 G170A-I177A 0.127591156015422 L183A-Y205A 0.127337570875277 G163A-G209A 0.127264873163441 L161A-K173A 0.127136185529586 N154A-I237A 0.127070460344807 G172A-G261A 0.126751860856502 L174A-L200A 0.126539196375235 L184A-L207A 0.126117317858518 N154A-A206A 0.126097062119224 S248A-A253A 0.125795615843707 V238A-A250A 0.12546110724094 L155A-Y160A 0.125372109365491 K173A-V267A 0.124977570023268 K173A-V260A 0.124149383837944 L207A-L234A 0.123673552388484 I151A-G240A 0.123053461890948 F153A-W158A 0.122920411398372 G163A-I175A 0.122872653150125 N154A-A239A 0.12242239286473 F153A-A239A 0.12229233209185 S185A-V260A 0.12196776633266 V165A-V260A 0.121635268135551 N251A-A259A 0.121466885664957 S185A-L207A 0.119650389485298 W212A-A263A 0.118965373127699 L234A-L258A 0.118796412019406 Y210A-A233A 0.118495352478594 Y210A-A256A 0.11802910050084 G199A-A256A 0.117882320328912 L148A-L200A 0.117829224533779 K173A-S181A 0.117551450129466 V203A-A250A 0.117252818517785 G163A-T178A 0.117241352900609 L156A-Y205A 0.116002211254981 L207A-L229A 0.115993308204726 L200A-A253A 0.115738736263319 L148A-L244A 0.114823151461291 K173A-G261A 0.113796297786915 G163A-V203A 0.113683109882264 A239A-A250A 0.113567644708196 A206A-N251A 0.113100488251862 L179A-L207A 0.112564680936993 L161A-S171A 0.112217311142716 V203A-G252A 0.110735017773771 T178A-G199A 0.110605908543539 Y160A-A233A 0.10969651739766 W159A-T201A 0.109336866486943 H254A-G261A 0.10931134129547 A164A-V176A 0.108252633350157 G202A-A263A 0.107981099488054 G209A-V260A 0.107271380280416 A253A-G261A 0.106492578695735 A239A-N251A 0.106392327283362 A206A-G252A 0.106132738146401 W236A-H254A 0.1058179284231 G170A-I175A 0.105182083908873 G199A-A206A 0.103838704481235 A164A-G172A 0.103659534622522 G252A-G261A 0.103513826270216 L174A-Y205A 0.103173546733401 L184A-V204A 0.103085248942138 L156A-L234A 0.10257546755436 Y205A-W236A 0.102445261685183 I175A-Y210A 0.102359446608196 V211A-G257A 0.102356920577777 M149A-A239A 0.102344597834962 T178A-A265A 0.1020742396944 A164A-G209A 0.101334952719267 L174A-L258A 0.0997843074444642 L161A-L234A 0.0993338689043881 L234A-L244A 0.0992386737433719 Y205A-Y210A 0.0989445396448989 L207A-A263A 0.0982583414663116 L155A-L229A 0.0979951030715172 G209A-L234A 0.0975515647131902 H150A-W158A 0.0974020852133191 L213A-L234A 0.0970884121941538 V204A-L229A 0.0967340001264887 T201A-A256A 0.0965196561251367 M149A-G199A 0.0954428232592432 L184A-L262A 0.0953500491088075 T201A-S248A 0.0944453747664404 L179A-L258A 0.094033704073909 L183A-A259A 0.0938256120471099 L213A-I255A 0.0938017842234385 L161A-I237A 0.0936391071020221 G257A-A265A 0.0927233403618546 L152A-I235A 0.0924234066957449 V211A-A259A 0.0921779656279464 V176A-L207A 0.0921295031520653 L184A-N251A 0.0920219664720598 L155A-I237A 0.0919149037021618 A164A-A265A 0.0919008270489465 G163A-K173A 0.0917573614184737 V204A-A256A 0.0912831382582935 T201A-M249A 0.0912310290368398 V176A-V204A 0.0911877104847107 G170A-V211A 0.0906031952331143 K173A-V203A 0.0901630396657627 H150A-A250A 0.0900968122723578 A164A-V203A 0.0898261260751574 L161A-L258A 0.0896861820719268 A233A-L244A 0.0885509142211838 S181A-G261A 0.0881315923988737 L179A-G199A 0.0880879690179057 L155A-L174A 0.0876550195030239 T201A-G252A 0.0875531579577701 A164A-M208A 0.0873810212814377 T201A-A259A 0.0870173462715682 Y160A-A206A 0.0868096369818208 V211A-V267A 0.0862637446923493 L184A-A250A 0.08618964170624 F153A-G162A 0.0861688452694183 E166A-M264A 0.0861190764730076 V165A-V203A 0.0859994663386825 I177A-V267A 0.0855983926737043 I177A-G257A 0.0855892542763659 Y205A-A250A 0.0849703983625401 W157A-I237A 0.0834061726169484 S181A-A265A 0.0833809929446772 G202A-A239A 0.0833103471899213 A239A-M249A 0.0830079233204585 I180A-A259A 0.0829199747023708 A256A-M264A 0.0826795668192576 G209A-A256A 0.0826424113466251 L155A-M208A 0.082571028509651 L183A-G257A 0.0824134570855388 W157A-T178A 0.0823292751230955 M208A-A256A 0.0821861248313454 I180A-A265A 0.0794621941685154 A206A-A233A 0.07877667753035 G202A-Y210A 0.0785758347583272 L161A-L262A 0.0774274298236577 I180A-A206A 0.0773395682879316 T178A-V203A 0.0772302448551789 L183A-V204A 0.0771945195419081 W158A-A233A 0.0769746160772127 W158A-I175A 0.0768720671641785 G162A-G209A 0.0766082656106517 I180A-S185A 0.0765350721613629 L244A-M249A 0.0762660980409908 W212A-I231A 0.0759217778818178 F153A-L207A 0.0751651189155507 A233A-W241A 0.0750455068299956 V211A-L229A 0.0749226228477102 A164A-T178A 0.074836076177152 L148A-G199A 0.0745402304536054 V238A-G252A 0.0744610256586267 L152A-L244A 0.0736860929672218 Y160A-Y210A 0.0734546164127297 I151A-A239A 0.0732277869041976 G172A-A265A 0.072306844880659 G162A-G261A 0.0722250584451398 Y210A-I230A 0.0720119126121443 L183A-H254A 0.0714794843432967 I180A-I255A 0.0708170466898306 Y160A-K173A 0.0707104692787581 G240A-G252A 0.0700175506610987 G162A-G257A 0.0699286755163853 L174A-M264A 0.0693347703216762 G172A-S181A 0.0689404588859484 L200A-W236A 0.0687077924836137 A239A-S248A 0.0681267048509611 L200A-I255A 0.0679184454576123 K173A-A206A 0.0677097815784879 G202A-M249A 0.0667749399239739 L156A-I231A 0.0662529951540876 L183A-I255A 0.0661721938920756 L213A-A259A 0.0659140497322079 A182A-G252A 0.065876792971074 V211A-A263A 0.0657870819226281 I151A-W159A 0.065573430345891 L152A-I237A 0.0655182020935894 A182A-A250A 0.0653011709576958 G240A-G257A 0.0648574209801636 A182A-L262A 0.0638881210582412 Y205A-I235A 0.063801133396006 L200A-N251A 0.0636595962719326 L213A-A233A 0.0635543701258445 G202A-G209A 0.0634467476492278 L183A-T201A 0.0633384557000607 L183A-L258A 0.0632779976084321 N154A-G209A 0.0632074061455843 A164A-I230A 0.0627650383161376 W157A-G209A 0.0615836379944206 T201A-F232A 0.061568577773609 L161A-I231A 0.0612254236909671 A164A-Y210A 0.0611512452523897 G199A-S248A 0.0611174797519558 S181A-Y210A 0.0609901791597469 A164A-L213A 0.0608751531173988 A182A-A263A 0.0601410591224403 Y205A-A239A 0.0601349175970025 G240A-I255A 0.060044690722457 V204A-A239A 0.0598201339597431 T178A-M208A 0.0588833808418493 W157A-S181A 0.0574744647718095 A250A-L258A 0.0572285888335733 L155A-A182A 0.0567326719065271 G199A-W236A 0.0561944792750582 L183A-G199A 0.0561926078746853 V165A-Y205A 0.0558982041606795 L200A-F245A 0.0552374584113764 E166A-G172A 0.0550916059969351 A164A-I175A 0.0550786583436853 W158A-G170A 0.0547196729265376 I177A-F266A 0.0544567815525089 I175A-L200A 0.054294608069867 G170A-A265A 0.0541068108216134 S185A-G209A 0.0539561465502829 L184A-V203A 0.053458219873022 G162A-S181A 0.0531353331313755 S181A-G252A 0.0529990416221933 L200A-I237A 0.0529046934851702 V238A-H254A 0.0525704627695998 L207A-A233A 0.0520529699064518 G209A-V238A 0.051909536351253 M149A-I237A 0.051467931193482 L156A-A233A 0.0513871147344025 S181A-V204A 0.051379576584436 I151A-V203A 0.0509186166735214 L183A-M264A 0.0504229219141288 F232A-L244A 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