[Wed Aug 24 18:31:45 2016] >Input file: TM2ZJSATD.pdb >TM segments: Y19-36_Y70-87_Y116-130_Y154-172_Y183-198_Y218-234_Y270-287_Y311-324_Y369-383_Y394-409_E27-55 MAIDEN score = -96.8610 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- P35Y-S71Y 10 I320Y-A372Y 1.44501198786322 I34Y-I72Y 1.32014478591484 I320Y-L373Y 1.25583284799579 I274Y-I403Y 1.1913070033172 A233Y-V27E 1.18683424424735 F73Y-I165Y 1.18153982610319 I275Y-I403Y 1.15694727907348 I120Y-I165Y 1.09593650898133 F128Y-W154Y 1.07119548020183 I34Y-S71Y 1.03556391214142 A277Y-E315Y 0.957029572358548 F396Y-L401Y 0.90907523546521 A278Y-E315Y 0.904537032916477 I274Y-V319Y 0.868009197725348 A229Y-T31E 0.855526300043552 Y80Y-I120Y 0.840234734256439 I77Y-L169Y 0.811238128180837 W196Y-A395Y 0.797513506511525 I120Y-V162Y 0.780364823520499 I77Y-I165Y 0.767072095074628 E195Y-A395Y 0.756900066967582 F128Y-V157Y 0.755189468472501 L197Y-V43E 0.744206813775825 F189Y-V407Y 0.741266128506187 W196Y-M42E 0.738198061250532 I77Y-L168Y 0.731554626844773 V193Y-M42E 0.729846533343652 I274Y-T323Y 0.725253410417631 F125Y-V158Y 0.721207314151501 F228Y-V405Y 0.71548731612407 P273Y-F322Y 0.699062125711386 L312Y-L380Y 0.698623377033109 F73Y-G164Y 0.691761544768294 Y80Y-L169Y 0.690470368226666 A408Y-F37E 0.683242770008639 I320Y-L375Y 0.679317185401196 Y80Y-L122Y 0.67902181353961 M78Y-A279Y 0.671403729407168 I274Y-F322Y 0.668248286201639 A26Y-Y48E 0.66595418146549 L25Y-I187Y 0.665858805998543 V271Y-I403Y 0.659082290768138 I85Y-F276Y 0.654631860656955 P79Y-Q126Y 0.650043790144416 F128Y-V158Y 0.641875546264463 F189Y-V404Y 0.641304455177569 T36Y-V157Y 0.640307373181666 G31Y-S71Y 0.639660282055329 L197Y-M42E 0.637154488982457 A278Y-V319Y 0.629863480275182 F228Y-I34E 0.629447456428642 Y28Y-L168Y 0.626536049350586 G31Y-F73Y 0.624173516742841 I77Y-I188Y 0.623660533277754 I72Y-A163Y 0.617999591803673 A277Y-F322Y 0.610691860193665 V405Y-I34E 0.605187207345549 I81Y-A279Y 0.604976403241289 A83Y-L122Y 0.60378812563145 I81Y-F276Y 0.599344082325524 I77Y-I187Y 0.588390940711237 F189Y-I403Y 0.579780564939959 R29Y-I34Y 0.572690819465515 F33Y-S71Y 0.57143940047071 P198Y-G46E 0.563009330734761 V234Y-G371Y 0.561779978879369 V194Y-L45E 0.557355321942127 V24Y-A172Y 0.555784843698344 P35Y-F70Y 0.545253780601313 A83Y-R116Y 0.543929213581798 F313Y-L380Y 0.53783777823247 F73Y-L168Y 0.528448555290825 A229Y-V28E 0.524967827499465 F228Y-L35E 0.507865551624605 D49E-F54E 0.502260986677077 F228Y-T31E 0.501466317778085 P273Y-E315Y 0.49694435186751 L409Y-I34E 0.496817284871922 A32Y-I72Y 0.49458679836591 A32Y-F73Y 0.489521009418523 V194Y-M42E 0.48947568827885 A172Y-I187Y 0.485190910924764 I274Y-L402Y 0.473995148150138 L25Y-A190Y 0.473115072785653 F276Y-F322Y 0.470338007391252 V316Y-L380Y 0.468389532535868 G76Y-I165Y 0.46661406205679 L30Y-F52E 0.466087424189041 I192Y-V404Y 0.463522588592845 P382Y-F396Y 0.462850539498041 L186Y-V407Y 0.456544866618962 R116Y-L169Y 0.452583012514596 P284Y-E315Y 0.449906182482653 V193Y-F41E 0.44885811348121 R29Y-Y48E 0.446839225781484 A227Y-V378Y 0.441862571738644 P79Y-I120Y 0.436652907172417 I81Y-G119Y 0.432256277753543 I34Y-F70Y 0.423945831808463 V193Y-A395Y 0.422393858369967 A32Y-F70Y 0.414782852843043 M78Y-I188Y 0.413637568553765 A124Y-V158Y 0.412564487550206 A395Y-L401Y 0.411878338107041 A278Y-I399Y 0.405227584219463 A124Y-V159Y 0.404521786187213 V193Y-L45E 0.400314768652281 V24Y-R29Y 0.400204817047852 T379Y-S397Y 0.399079302979415 I192Y-G400Y 0.397746837377561 A121Y-V162Y 0.392045606800528 A408Y-T38E 0.391505720958106 V319Y-A372Y 0.390263272365179 I120Y-A166Y 0.385122380429978 Y80Y-Q282Y 0.383675354857068 Q383Y-G398Y 0.37948060109884 V378Y-L401Y 0.37528032069155 A74Y-A124Y 0.373266582316096 F128Y-F155Y 0.371696024940025 Y324Y-L369Y 0.370900691129948 I274Y-I399Y 0.370743882841632 M171Y-I187Y 0.370538245075071 I72Y-I165Y 0.369480896816573 G31Y-I72Y 0.36793266628271 L169Y-T184Y 0.366871523068843 A408Y-I34E 0.365375870256677 V193Y-T38E 0.35780954291981 W196Y-V43E 0.357396324271757 I72Y-G164Y 0.355867622849028 T36Y-I72Y 0.354046592774369 M78Y-I165Y 0.353422197322821 L321Y-A372Y 0.353139463583711 I394Y-G400Y 0.342334718006246 M231Y-L375Y 0.340701424325917 V378Y-L402Y 0.339603008081097 P79Y-Q282Y 0.339001714760939 E29E-F37E 0.337938360199733 V225Y-M42E 0.336433635386711 A27Y-A172Y 0.336001102769127 V319Y-L375Y 0.335331057789535 V404Y-T38E 0.333152879630458 P79Y-L122Y 0.331795048307449 R29Y-L45E 0.331435460350859 G270Y-A408Y 0.330058532249741 V225Y-T31E 0.325456589737975 R29Y-I187Y 0.324924280271992 P79Y-P284Y 0.324922962909933 E315Y-Q383Y 0.322912184820701 A74Y-Q161Y 0.321821587062933 A408Y-A40E 0.320930713907654 A278Y-G311Y 0.319507362955505 V225Y-L35E 0.316300088295569 Y324Y-G371Y 0.313620673242061 G183Y-G191Y 0.313266862721101 G230Y-G376Y 0.311823384266195 V316Y-L377Y 0.3115190216486 A190Y-A395Y 0.308549194747926 A26Y-G191Y 0.307524879690263 S185Y-I275Y 0.307408564824829 T379Y-L402Y 0.305539944630228 G230Y-G406Y 0.304753856756109 Y28Y-P35Y 0.303809422249502 Y80Y-G118Y 0.303634822877901 A190Y-L45E 0.300393046278951 T82Y-G119Y 0.298547262488892 G76Y-G183Y 0.298302006512706 M171Y-G183Y 0.297404680405347 G31Y-G191Y 0.296735539396803 V24Y-M171Y 0.295933787006177 T82Y-P273Y 0.295910526893588 S185Y-V407Y 0.294338865218506 V193Y-V404Y 0.291929940804364 G118Y-A166Y 0.290508150504676 A26Y-L45E 0.288341118313579 P273Y-T379Y 0.287215575953849 A74Y-A163Y 0.285544985973731 L281Y-I314Y 0.283939740309059 L380Y-G400Y 0.278775776009159 G230Y-L402Y 0.2779895183659 V316Y-V378Y 0.276485732479481 S71Y-G127Y 0.268854017934716 A233Y-G371Y 0.266647384199243 T82Y-P284Y 0.266206139278345 A277Y-G376Y 0.265993612713888 G270Y-G376Y 0.265429369002397 L381Y-G398Y 0.264360316868764 A232Y-I34E 0.257519498928218 F20Y-G183Y 0.255928737015265 E195Y-T38E 0.253579445225118 G123Y-L168Y 0.253318223102633 G119Y-W170Y 0.251150591730622 Q126Y-G164Y 0.251020568834771 R29Y-M171Y 0.249436781961423 L30Y-P35Y 0.249059319751831 Y80Y-Q161Y 0.248524198952364 Y80Y-S185Y 0.248168223313154 Q126Y-P284Y 0.248064607208919 G118Y-L169Y 0.247797613503343 G118Y-Q126Y 0.247439816815009 P79Y-L168Y 0.246426422275241 P273Y-L375Y 0.245140679114689 T379Y-L401Y 0.242875810872542 L312Y-Q383Y 0.242571784889927 G123Y-I165Y 0.242521445229553 L186Y-T38E 0.242142228869733 P35Y-I72Y 0.240471918748187 G191Y-F41E 0.239743705011069 Q282Y-L287Y 0.238406894444969 W196Y-G46E 0.237428138922446 R116Y-Q161Y 0.235864985111899 A279Y-F322Y 0.233053107155161 E315Y-T323Y 0.232153658446068 I81Y-I275Y 0.230955169693253 P79Y-L287Y 0.230918801657846 T82Y-L122Y 0.227521404002012 Y48E-R53E 0.226624926601741 L75Y-T82Y 0.226115396089444 P79Y-L130Y 0.225287362621726 A27Y-G164Y 0.224079315689757 G230Y-Q32E 0.223869499625698 A190Y-F41E 0.223380515452828 L317Y-G376Y 0.222468152463768 V271Y-V407Y 0.222417102506675 R29Y-M42E 0.22115986988747 G76Y-I188Y 0.219908025109699 L221Y-P382Y 0.219050762567415 V271Y-I399Y 0.218774159582614 G376Y-Q383Y 0.21728484591186 I188Y-V271Y 0.216166375011769 V225Y-G30E 0.214150059396942 T36Y-W154Y 0.213506763133418 R116Y-M171Y 0.213104824691169 M78Y-V162Y 0.211421188746611 S185Y-V405Y 0.211411833689524 F73Y-V158Y 0.21070442737307 Y28Y-I187Y 0.210183145179901 L380Y-G398Y 0.209888967588139 V194Y-L47E 0.209827190630448 T82Y-I280Y 0.209674017218166 M78Y-Q161Y 0.20936778296367 G76Y-V162Y 0.209246540106958 F223Y-G398Y 0.208760113890431 S185Y-P273Y 0.208222300412418 A121Y-A163Y 0.207660342092634 A32Y-Y48E 0.207500651334904 G230Y-T379Y 0.206536112093971 M78Y-R116Y 0.206496226136117 V193Y-L401Y 0.206321681757123 V316Y-T379Y 0.206260962915894 W196Y-I44E 0.20600262521254 W196Y-F396Y 0.205699712869368 V316Y-L373Y 0.205544529556138 V24Y-L168Y 0.205453411677771 T323Y-L375Y 0.205173852217527 A227Y-S397Y 0.204498206299993 F396Y-V405Y 0.204336689106529 F73Y-V159Y 0.203391857999742 Q126Y-F155Y 0.203290713723593 V405Y-G30E 0.203217504144289 A27Y-S71Y 0.203122960241258 M231Y-L381Y 0.20255412517996 E315Y-P382Y 0.20250903266645 G76Y-F189Y 0.201570910564655 I187Y-T38E 0.201500950444836 I85Y-T184Y 0.200924783721194 G31Y-F52E 0.200728321921451 T184Y-G406Y 0.200618320452064 I275Y-G406Y 0.200151753321843 M78Y-I275Y 0.199294034959607 P273Y-V319Y 0.199010589901063 M231Y-Q32E 0.19885497583606 G400Y-V407Y 0.197463670572473 G406Y-T31E 0.196376159154735 V193Y-F37E 0.196099434090668 G76Y-L168Y 0.195954307488159 Y80Y-R116Y 0.194989201878522 I224Y-F396Y 0.194180961354414 P273Y-G400Y 0.193937814755241 A190Y-G400Y 0.193286895069494 I224Y-V404Y 0.192915245586555 S185Y-A279Y 0.191725782390887 T82Y-L281Y 0.190998462485912 T82Y-A124Y 0.189818416295261 F73Y-M78Y 0.189780053425447 T184Y-A279Y 0.189339292116716 V405Y-M42E 0.189062480769812 G119Y-L168Y 0.188369448070983 G270Y-I275Y 0.188072443268765 M78Y-L167Y 0.186053482448423 T21Y-I188Y 0.185872017375813 T379Y-G406Y 0.185180842758792 T184Y-A408Y 0.185053760877902 G400Y-T38E 0.184465090113968 V234Y-G270Y 0.184073436177943 G123Y-V158Y 0.184066248157722 G118Y-G164Y 0.18376350079809 I192Y-G398Y 0.183690482048437 S185Y-A278Y 0.183487809036023 V319Y-T379Y 0.183377486716154 L401Y-M42E 0.182867955543 L281Y-L318Y 0.182544730770091 G30E-T38E 0.182420484658305 F33Y-F52E 0.181768506041685 I188Y-A279Y 0.181653362730066 P382Y-L402Y 0.181151191503792 I280Y-T323Y 0.180120456917969 I117Y-W170Y 0.17987877629964 G230Y-L381Y 0.179514377482905 G230Y-L373Y 0.179139213540201 I117Y-A124Y 0.178698865604323 L221Y-S397Y 0.178603390224207 G270Y-L373Y 0.178579128142337 G31Y-L169Y 0.178499451528649 T82Y-I285Y 0.177983293788304 F70Y-Q126Y 0.177404044746893 I86Y-A279Y 0.176778783146633 F322Y-G371Y 0.176084898910852 I77Y-A172Y 0.175519682781882 A190Y-Y48E 0.1752560417633 P79Y-L167Y 0.17318538804661 G230Y-P382Y 0.172760082403965 A395Y-T38E 0.172353604219182 V271Y-G406Y 0.1719615317301 A227Y-I34E 0.171714249232386 E195Y-G400Y 0.171199730706052 G270Y-A277Y 0.170142168143328 A166Y-T184Y 0.169955564539919 A233Y-G30E 0.16952722491803 G371Y-V378Y 0.16936326489388 A121Y-T160Y 0.168789536959138 T184Y-I274Y 0.168530869725521 Y80Y-G127Y 0.167813846372933 G76Y-T160Y 0.167209561508569 A232Y-G398Y 0.166981175659228 I280Y-I320Y 0.166716603612373 G31Y-A163Y 0.166510249057911 G31Y-Q161Y 0.165836333066408 G270Y-A279Y 0.165710200523227 F20Y-A172Y 0.16532773057646 A84Y-G119Y 0.165180229388413 I188Y-A278Y 0.165073995732922 A26Y-G164Y 0.165042259043133 I77Y-I85Y 0.164735457794351 A278Y-L318Y 0.164683035867704 V271Y-A279Y 0.164426791439734 G311Y-G400Y 0.164354971079601 A190Y-A40E 0.164123532290844 A229Y-V27E 0.163599206882163 A26Y-V43E 0.163035152041201 G76Y-G119Y 0.162787789392105 A26Y-G46E 0.162746628425901 G191Y-G46E 0.162690048000593 F219Y-F396Y 0.162413672506942 I77Y-A190Y 0.162249816438508 G398Y-G406Y 0.161877117290884 A74Y-F128Y 0.16160590182994 L375Y-S397Y 0.161295456559545 A83Y-G127Y 0.161173680496344 G119Y-G183Y 0.160986595468827 W196Y-L35E 0.160948884370632 W196Y-D49E 0.160661309995196 G118Y-A163Y 0.160549553542331 I188Y-T38E 0.159978762894667 A26Y-F41E 0.15848865661145 A172Y-T184Y 0.15842410164327 W196Y-L39E 0.157835185586075 A190Y-E195Y 0.157158479110719 W196Y-L45E 0.156760723179014 A26Y-F33Y 0.156375329172213 G398Y-A408Y 0.155768180312635 A27Y-I34Y 0.155418510364546 F313Y-S397Y 0.155208704530043 L168Y-S185Y 0.154169503405942 G76Y-G127Y 0.153019752723266 G270Y-I403Y 0.152086075163529 I224Y-I403Y 0.151351022508835 A232Y-E29E 0.151183317170767 M87Y-I280Y 0.151008694208279 G376Y-G400Y 0.150102238906182 A408Y-G30E 0.150016714832806 I274Y-V378Y 0.149945032891987 A74Y-I188Y 0.14986409221376 A74Y-I120Y 0.149662770906788 I224Y-G398Y 0.149602171682127 A229Y-V404Y 0.149213506103591 V404Y-I34E 0.14884381539865 R29Y-A172Y 0.148683269974275 Y28Y-W170Y 0.148410762730108 G406Y-F37E 0.147953235674596 G191Y-G398Y 0.147789476823908 M231Y-G376Y 0.147251849603903 W196Y-S397Y 0.147220611740544 M231Y-A372Y 0.147037156430439 G191Y-T38E 0.146770876738315 I86Y-I120Y 0.146389048779668 I120Y-G164Y 0.146353399417023 V378Y-S397Y 0.145747011202591 S71Y-V162Y 0.145184299620229 G371Y-T379Y 0.143895963387534 I81Y-R116Y 0.143714537983797 I72Y-I77Y 0.143385997636451 G127Y-F286Y 0.142840922272793 G76Y-S185Y 0.142502652218145 T82Y-I274Y 0.142413325106585 V404Y-A33E 0.141622690943727 V27E-Q32E 0.141232666334814 Y28Y-I192Y 0.140685568306288 L186Y-I44E 0.140183238636863 A233Y-V378Y 0.14009906684812 G230Y-G371Y 0.139907175491317 R29Y-A74Y 0.139865719020445 I224Y-L401Y 0.139820860988793 Q161Y-A166Y 0.139710645603643 L22Y-I188Y 0.13963898591867 P35Y-T160Y 0.139373050087721 G406Y-G30E 0.13926663600878 F189Y-F276Y 0.138641165894269 L130Y-W154Y 0.137790313859682 G191Y-A40E 0.137752344889001 V316Y-S397Y 0.13765327299029 V404Y-V43E 0.137630730501084 I77Y-A124Y 0.137388830924485 G31Y-M171Y 0.136951351318817 I77Y-G123Y 0.136004591200088 A229Y-G371Y 0.135891598521346 V319Y-V404Y 0.135832307489363 L22Y-G183Y 0.134076106270189 F189Y-V271Y 0.134045019615266 L221Y-L381Y 0.133853536312218 V405Y-V28E 0.13383444353493 G230Y-G398Y 0.133821587497216 I403Y-L35E 0.133586086841836 F41E-G46E 0.133468324113343 G270Y-L321Y 0.133342091497009 R116Y-A124Y 0.133090210287989 A84Y-S185Y 0.133035116300148 A227Y-G376Y 0.132453763136375 A83Y-A166Y 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