[Wed Aug 24 17:16:15 2016] >Input file: TM3AYFATD.pdb >TM segments: A20-42_A250-272_A304-332_A345-372_A385-410_A428-454_A471-490_A499-523_A541-562_A569-592_A614-639_A642-669_A688-713_A734-762 MAIDEN score = -132.9780 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- S257A-V576A 2.49506687869445 A592A-I616A 2.34565162436714 I589A-I620A 1.78837710671041 V310A-A760A 1.60830137666638 V251A-A637A 1.39243516116139 I265A-I583A 1.35060319470016 I507A-E512A 1.30315849244938 L26A-T547A 1.30048919156479 V312A-W362A 1.26957281725089 M692A-I748A 1.26020035278653 H559A-N634A 1.21749953139057 I372A-W448A 1.20835057128799 L37A-I555A 1.16020333063437 I555A-F577A 1.15425157869444 W505A-I557A 1.15358649815128 F486A-W505A 1.14327724273417 L449A-I478A 1.1216844986921 H559A-I571A 1.07193088190811 Q319A-L356A 1.07136618760565 F483A-W510A 1.07031682480514 F489A-W502A 1.06308503684169 F316A-W362A 1.04965158643916 L585A-L623A 1.04065936459344 A575A-P636A 1.02166060711093 Y329A-F348A 1.01557477021185 F501A-Y561A 1.00685481785137 F322A-Y354A 1.00602765432635 V624A-F629A 1.00039280532327 Y308A-F394A 0.996708688896822 L261A-L580A 0.993641241539903 T618A-V659A 0.988484706608366 E591A-A619A 0.962922984937785 F317A-V753A 0.962541951823351 Y562A-I571A 0.959474186021351 I548A-E581A 0.958661743533691 I703A-R742A 0.957646506766842 F316A-V753A 0.947602344003006 F614A-M662A 0.945577832215035 F515A-Y660A 0.938458808884398 F486A-W502A 0.931162687742663 F515A-V520A 0.925428119975635 E642A-L647A 0.91535711773712 V517A-F546A 0.911652279286579 Q357A-L433A 0.904800650528138 L693A-F749A 0.897539754159742 I363A-Y660A 0.885004060039177 H508A-I557A 0.882623537810692 Y430A-I507A 0.874110470638842 I372A-V452A 0.86919088814764 F646A-L705A 0.866535621446305 I350A-Q408A 0.859336797120015 I30A-L550A 0.847617006332644 W253A-L635A 0.835838519498841 L647A-L705A 0.834592846817092 H653A-L697A 0.832716309227393 Y561A-I571A 0.812810538343077 F309A-L668A 0.803411375308191 F322A-K352A 0.801444592457406 L697A-D746A 0.790679501464299 I372A-I451A 0.786183826349268 W367A-A519A 0.785668663156254 I555A-L573A 0.777607067066235 I633A-V638A 0.777366070228212 V442A-Y484A 0.773455713103776 I506A-V511A 0.772166534824768 T704A-L739A 0.771413460847587 L549A-P584A 0.768566306087939 W504A-I557A 0.766860581162468 I350A-W409A 0.76626674895541 V480A-I514A 0.760679343137321 S689A-I751A 0.757851808386849 L588A-I620A 0.755851108497253 W253A-I571A 0.753547489430746 E642A-M713A 0.752807821326556 L326A-F348A 0.752420498978974 E512A-V517A 0.750977394044041 F646A-V708A 0.74713945934879 F629A-H651A 0.74608380337178 L315A-W362A 0.745840238969449 L693A-V753A 0.737085951467441 S552A-F577A 0.736877718686689 Q645A-Q712A 0.735467118332364 L615A-F663A 0.735245091023804 S254A-L635A 0.734047805115263 I490A-W502A 0.733617289815347 S34A-I555A 0.730615731322832 V521A-L542A 0.728612866551631 I633A-T648A 0.724065808278465 A487A-W505A 0.722993013688052 W621A-A626A 0.722985314279326 V708A-L738A 0.722866193601817 L389A-I451A 0.722846571145568 L588A-L623A 0.721795511406312 I25A-T547A 0.717601702425389 I589A-L623A 0.715674578743751 K304A-V390A 0.715181357280296 F317A-V754A 0.711168199526013 C690A-G752A 0.70983963841455 L400A-W437A 0.709516519239296 T618A-Y660A 0.707905845319882 W621A-G658A 0.703616319766039 V310A-I761A 0.703006298377394 W621A-V659A 0.700836345649724 L509A-V554A 0.68628271925891 L647A-L706A 0.678258667992513 V480A-G513A 0.675749854880057 W570A-A575A 0.673331365279481 F646A-Q712A 0.673147736010062 M445A-P481A 0.669782580789837 L473A-V521A 0.668980772497939 F614A-I665A 0.663079863693683 L697A-F749A 0.660686225926364 A487A-W502A 0.658243914869183 V312A-T365A 0.657905676222938 L315A-L358A 0.655496226907778 W621A-A654A 0.654349948969449 W621A-A655A 0.65383901497204 W367A-A664A 0.652619951270185 M405A-L433A 0.652039451507886 W253A-A575A 0.651492795755357 S33A-G551A 0.647029474028559 G625A-V659A 0.642344483427415 A650A-V701A 0.642231027561809 A626A-V659A 0.636986327641964 L400A-I440A 0.634475501666504 V701A-L706A 0.633421612250904 I695A-F749A 0.630122479658231 Q545A-L550A 0.628584804720596 W253A-V576A 0.62718938382299 Y330A-L739A 0.626734127431088 W367A-V667A 0.624832586363054 L449A-P481A 0.621076624109739 F614A-F663A 0.619596316488427 L449A-A477A 0.619471754768928 I633A-E642A 0.618208039816405 F309A-G369A 0.616892138894469 A579A-N634A 0.616713051635219 P707A-L738A 0.615004500567335 G41A-F501A 0.614850689372117 F309A-A366A 0.611507604524172 W505A-W510A 0.61095928990763 G556A-V576A 0.609917337737848 L472A-V521A 0.608578139248383 V480A-W510A 0.603757283452118 F688A-A755A 0.601621115209979 V624A-A655A 0.599175610593853 M692A-F749A 0.597682200536866 T320A-H355A 0.595644004565766 L446A-P481A 0.590511217844409 M370A-L668A 0.58679162810053 A364A-V511A 0.583972487172083 Q357A-I431A 0.582033182015001 H643A-T648A 0.581452456037926 G513A-F518A 0.57857141272136 A650A-V702A 0.575116656258341 T618A-M662A 0.572947648346917 F688A-G752A 0.566409036022997 L400A-L441A 0.563626658055827 F316A-A359A 0.563459162256866 N622A-G627A 0.559800434312981 Q386A-I451A 0.559325145301189 V390A-I451A 0.559046696289081 T704A-V741A 0.558885520248125 G353A-Y430A 0.556119927996772 A250A-I571A 0.553505218262184 H651A-V701A 0.553041373092618 I260A-V576A 0.551695202033782 V36A-I555A 0.551030455859383 L26A-F544A 0.550819505219747 L356A-E429A 0.546194590528247 A579A-F631A 0.532074186881479 W367A-L668A 0.528857122438708 A655A-Y660A 0.526691495290565 V312A-I363A 0.524793633269748 F629A-A654A 0.524558377414125 F309A-A760A 0.523981710358403 K23A-Y543A 0.522377131867033 F489A-A499A 0.522133704274433 Y484A-I506A 0.521273869762618 L509A-I557A 0.520603696050168 M657A-M662A 0.51915051466417 F264A-L580A 0.51512487762482 S404A-G434A 0.514727840096913 A619A-V659A 0.509070665961949 F614A-C690A 0.508967056841737 Y561A-G574A 0.508607067129613 F629A-A650A 0.508081159622377 A626A-F631A 0.507155106772778 A654A-V701A 0.504584885197099 L326A-A351A 0.504235059103745 V310A-L757A 0.503630540568778 Y562A-G574A 0.503366718622924 G323A-K352A 0.499399643686997 L623A-V628A 0.498988326732991 Q319A-Y354A 0.497353062389167 Y484A-W510A 0.496646258993632 A592A-I620A 0.49628123032913 G699A-R742A 0.495816474435564 F316A-L358A 0.494589653211266 V452A-A477A 0.492658173052583 G553A-S578A 0.490457642012761 T586A-L623A 0.490420237410333 A477A-F518A 0.490269392318634 I490A-R503A 0.488895521859817 R435A-I490A 0.487233719158315 F453A-F474A 0.484474047542507 L393A-L447A 0.480598229078152 A324A-D746A 0.480494753821974 G353A-E432A 0.480105121033317 I30A-T547A 0.479274256760116 I514A-A519A 0.478220427908536 L473A-V522A 0.477105780291213 S476A-F518A 0.47654800626396 S617A-V659A 0.475846026540634 L22A-Y543A 0.470577630112188 F486A-I506A 0.469087232957602 I633A-L647A 0.467827878230733 F646A-L706A 0.467371374102675 S33A-L550A 0.465434652180592 A305A-M370A 0.464289519299408 L693A-I748A 0.463851772529724 M700A-L705A 0.463331128973707 S476A-I514A 0.462537156231707 L647A-I710A 0.462168313834289 I703A-L738A 0.462139708144896 I695A-P745A 0.461425465939313 F483A-W505A 0.460293499656322 F322A-A351A 0.459654516304415 E516A-L549A 0.45885923435992 Q645A-M713A 0.457083911666061 I555A-S578A 0.450539922438503 K23A-Q545A 0.448775811661901 E516A-T618A 0.446582386631312 G696A-T747A 0.445413767906544 I431A-R503A 0.438503727100037 V390A-W448A 0.438428435539423 T29A-T547A 0.437496181130432 S313A-L757A 0.437239772281615 I557A-F577A 0.435628978754163 A364A-I514A 0.435015159662762 V708A-V735A 0.433633973388165 S254A-A575A 0.432161173062745 L37A-V554A 0.432024522430925 D500A-H508A 0.431442965805088 L26A-Y543A 0.431045835965275 G699A-T704A 0.429173912035856 A592A-L615A 0.427306269367278 L705A-R742A 0.426154583162136 Y308A-A366A 0.423690580000213 A654A-L697A 0.423466639226231 L473A-F518A 0.422841862910965 H355A-H653A 0.422055341247227 K23A-F28A 0.419614544201396 T331A-L739A 0.419422048781882 H355A-M657A 0.419203224638131 I363A-M662A 0.41908399758307 M321A-L750A 0.418738813106532 T547A-E581A 0.418736624764956 L697A-P745A 0.4170456825291 P649A-L705A 0.416998555278068 F317A-L757A 0.41699170014695 T320A-L750A 0.416957501597162 I350A-M405A 0.414788461102596 I30A-G551A 0.413688427498428 W367A-I514A 0.413564901285934 S689A-L756A 0.412615073744181 L22A-F544A 0.412555446807797 E332A-P347A 0.411397382186951 V399A-S404A 0.41006109713202 T29A-L550A 0.408923954459392 L588A-N622A 0.407145470582644 L315A-F361A 0.406801514291471 L26A-F546A 0.404665278789496 S313A-L756A 0.40454917896832 A366A-V667A 0.404134792337059 L356A-Y430A 0.403322290908715 A626A-A654A 0.402377279283761 I363A-M657A 0.400193103920893 V520A-L542A 0.398769976522039 S552A-H559A 0.397433826861505 S254A-H559A 0.396889865995313 S257A-H559A 0.396852368032971 A305A-I372A 0.394779355453299 M700A-P745A 0.394470234191144 V708A-L739A 0.394403484764677 H653A-N694A 0.392441795886679 L615A-M662A 0.391962353794158 V442A-W448A 0.391792241557123 A256A-V576A 0.391747820416309 V624A-A654A 0.390245293143026 F264A-L269A 0.38967249077294 I589A-A619A 0.388264939865788 V582A-L623A 0.387041798554798 F483A-I506A 0.386474889361911 A305A-V390A 0.386289269744794 I571A-P636A 0.385678641936725 H559A-W570A 0.385641722617666 A654A-V659A 0.385428584029205 W621A-V628A 0.384901580053447 I695A-I748A 0.384485087726011 H355A-M656A 0.384358760122475 V251A-P636A 0.383958240545124 H643A-H651A 0.383430271717635 H328A-D746A 0.383158656371056 F264A-I583A 0.380440340341105 A314A-A366A 0.378596622052008 A650A-L705A 0.378556090848065 Q408A-G434A 0.378518947229398 A314A-A359A 0.37830939014282 F646A-I710A 0.378247027791946 W504A-L509A 0.376305807616229 A327A-R742A 0.375939033716521 N622A-H653A 0.371104875983741 K352A-E432A 0.370554166067403 W395A-M445A 0.369442013518166 H653A-V701A 0.368402939520831 W691A-G752A 0.366275169617752 W504A-H560A 0.365041637576939 G40A-W570A 0.362601354569501 M657A-L697A 0.361972502735042 E642A-P649A 0.361375568796249 V318A-L358A 0.360698446345204 A366A-L668A 0.358182710112627 V312A-Y660A 0.356583726145453 Y329A-V735A 0.356546179192347 A575A-L635A 0.35610395400196 E516A-T547A 0.354685346704192 L647A-V708A 0.352819185225269 H559A-L573A 0.351733245843452 W437A-V442A 0.350972960473344 L397A-G444A 0.350876965542047 Y354A-M405A 0.350848145156532 L549A-V554A 0.350381850748986 V390A-W395A 0.349739826801595 W437A-M445A 0.348549020709943 I268A-I583A 0.347424913296805 P707A-Q712A 0.347266031481344 H508A-H653A 0.34591342531759 Q438A-P481A 0.345243734816069 F316A-I363A 0.343586268175748 L325A-A351A 0.342510513371091 W367A-V522A 0.342457889617823 V311A-W362A 0.342356114210685 S257A-A572A 0.34102857143411 I345A-W409A 0.340224168776757 A327A-L739A 0.338367659436676 G553A-E581A 0.337169037178507 I490A-A499A 0.337101684986228 K352A-Q408A 0.336387322227494 M445A-W510A 0.334530291186765 Q408A-E429A 0.334490944794567 G556A-L573A 0.333976979382368 F317A-L750A 0.333913417856078 V628A-M657A 0.332344908415704 T331A-T704A 0.33192927541747 I548A-I583A 0.329799283107349 G369A-A519A 0.329738930099794 S313A-W362A 0.326322044207518 G371A-Y475A 0.325539029102696 T586A-T618A 0.324957929012887 V624A-Y660A 0.324934661149688 K23A-H471A 0.324030408474254 L449A-R454A 0.323023057651076 G644A-A650A 0.322554700759537 I372A-V390A 0.322543374350174 G40A-A572A 0.321913261790466 Q545A-E591A 0.320755860333192 L326A-K352A 0.320249494572064 S617A-M662A 0.320132044077991 Y329A-V741A 0.319489330174903 K385A-R454A 0.318455904346317 A396A-G444A 0.317899854538438 E591A-I616A 0.31724849377882 A579A-A626A 0.316221106120413 G551A-G574A 0.314616827052495 G369A-G387A 0.314503905945809 S257A-F577A 0.314458076599657 S617A-A664A 0.314426690582889 W253A-A637A 0.313562076551504 G625A-G699A 0.311521256642426 I360A-V511A 0.311465349622255 G434A-A487A 0.311433948808212 H508A-G630A 0.311364576506743 Y562A-S639A 0.310284351937227 F546A-G551A 0.310095141302231 S257A-A637A 0.30975868828884 F361A-E432A 0.309562692657768 H559A-G644A 0.309323249912002 G40A-H560A 0.308674587416498 D391A-V398A 0.308577207291625 L393A-W448A 0.308209595504862 G551A-A579A 0.305550645455423 M656A-G661A 0.303858207896515 M370A-D391A 0.303409131631132 A619A-A666A 0.303354520198448 S617A-A655A 0.303329524138846 Y270A-P584A 0.303071069780633 G661A-G752A 0.302864446304019 H651A-G699A 0.302509326315825 H355A-G652A 0.302419375294197 G558A-I571A 0.301914230948343 G369A-G444A 0.301088924525117 R503A-H508A 0.298878660692134 S34A-G556A 0.298781767515597 L711A-V735A 0.298593180930302 A619A-G661A 0.298041517465406 L397A-L441A 0.297050280716393 H653A-G658A 0.297003230487068 G371A-S476A 0.296313167452712 H508A-S552A 0.296137995977683 A626A-H651A 0.295160410867555 G627A-H653A 0.295067682169563 G306A-S313A 0.294859461163459 L315A-A359A 0.293922811472907 I703A-V741A 0.293762845092515 L368A-W448A 0.293458120339914 A575A-N634A 0.293196459112595 H643A-G652A 0.292215601749371 V442A-I485A 0.291516617302346 G658A-C690A 0.291462155828668 L585A-A619A 0.290227803116666 Y561A-G644A 0.289754582134066 Y308A-T365A 0.288621491910234 T320A-P745A 0.28800206430743 P707A-P745A 0.287760078560559 A250A-L635A 0.287556934335147 A314A-A755A 0.287136056922003 G403A-L441A 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