[Thu Aug 25 00:41:06 2016] >Input file: TM3EMNATG.pdb >TM segments: X5-24_X26-33_X39-46_X56-62_X69-76_X81-87_X96-102_X111-118_X124-131_X136-144_X150-157_X166-173_X179-187_X189-196_X202-210_X218-227_X231-239_X242-252_X254-262 MAIDEN score = -30.2437 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- M155X-Q166X 10 Q226X-C232X 10 T250X-K256X 5.41315871506538 A222X-K236X 4.31886342399929 S57X-N76X 4.14011086775065 S43X-L58X 3.65377451772372 C127X-R139X 2.95264619535857 T248X-T258X 2.92204771992914 V227X-C232X 2.22508420021298 M155X-S167X 1.70754257678786 I114X-V129X 1.55498792425221 I194X-V206X 1.240050740433 L180X-I194X 1.23163724250962 W75X-L81X 1.22113052285707 F18X-K236X 1.21504776199628 C232X-Y247X 1.20987361414222 I85X-F99X 1.20858387831056 L208X-F219X 1.18898763612543 F99X-K115X 1.1615971653206 I138X-F157X 1.14933185591276 I243X-L262X 1.131855244515 F71X-I85X 1.11028643342731 Y225X-C232X 1.10275839888944 H181X-I194X 1.06849773525713 L58X-W75X 1.04632892200953 L97X-T116X 1.0249032928661 F131X-P136X 1.01182320231462 Y22X-T258X 1.00664840983261 F99X-I114X 1.00188549183862 Q179X-Q196X 0.985988516684115 V206X-F219X 0.958359494289886 E189X-A209X 0.953083969571342 N124X-V143X 0.937164215623461 E73X-L81X 0.889111119975 G117X-C127X 0.885286475041137 L125X-L142X 0.882725433370538 V227X-F233X 0.845805421772567 V237X-I243X 0.84097892942518 V87X-L97X 0.836020156463141 K224X-A235X 0.827700629916563 F71X-T83X 0.812435720955443 F41X-T60X 0.795600715968881 T182X-F190X 0.78829960011696 L144X-L150X 0.749808859706361 L69X-V87X 0.717091574705746 V17X-T204X 0.715857888702817 I221X-V237X 0.712552300856796 F24X-L262X 0.697441410984719 V143X-L150X 0.690567529443442 T70X-V87X 0.690477839222069 Y22X-L259X 0.681413734041482 F157X-Q166X 0.678893431388554 L202X-A223X 0.666347112006132 Y22X-L245X 0.663291786218208 V184X-F190X 0.661781495795683 L245X-L262X 0.658095272433556 T6X-V143X 0.657694355724622 V206X-I221X 0.649953654927357 G21X-S234X 0.643965514512292 V129X-I138X 0.633786864646096 R218X-N238X 0.625425942388897 G45X-S57X 0.622705446121128 F169X-V184X 0.622348387395793 A231X-Q249X 0.614382335632213 A14X-N207X 0.613309908747166 F24X-A261X 0.610046269724508 F18X-A223X 0.609602829320512 A209X-F219X 0.606129652294994 F24X-S260X 0.600382979693046 L251X-L257X 0.593445541889975 G21X-Y247X 0.5775289489668 L242X-L262X 0.576940552146117 V237X-L242X 0.566058588816278 A151X-F169X 0.563993260511479 F190X-W210X 0.561813951737064 F99X-A112X 0.560309576400499 A235X-L245X 0.558400303426732 F18X-A205X 0.557965386363469 T250X-L257X 0.556931007923305 S46X-G56X 0.556929077067462 P5X-L10X 0.555185274001101 L69X-I85X 0.554157340743254 L150X-V171X 0.551177185341925 G126X-R139X 0.548125362652171 K236X-L242X 0.543005540066994 F233X-L245X 0.539949855446905 G23X-S260X 0.53705193518454 F169X-D186X 0.533104981561158 F41X-L58X 0.527016643779047 F18X-A222X 0.522793780871235 A170X-N183X 0.516953586676288 G191X-W210X 0.500215228098345 Y7X-Y153X 0.497698302564355 G11X-S193X 0.491588701990479 L39X-Y62X 0.489025512253619 A14X-T204X 0.472930505553312 F169X-N185X 0.469699262888215 L125X-V143X 0.467258300544442 Y22X-A261X 0.46355259828783 S193X-A205X 0.457755598904128 L97X-I114X 0.456848622438066 T204X-I221X 0.456168289312465 L58X-E73X 0.452515224677626 V143X-G152X 0.452099041527347 A231X-Y247X 0.451013685533962 F233X-T248X 0.448823775534283 T86X-F99X 0.448410923159412 F71X-T86X 0.448338099555829 I138X-M155X 0.447622381920207 N238X-I243X 0.445354435812263 L10X-H181X 0.434150569316549 S193X-L208X 0.431059316947223 A170X-H181X 0.428523401945221 Q166X-D186X 0.428064349573433 D9X-H181X 0.416556927664828 Q166X-N185X 0.414490988011789 K96X-N124X 0.401726979428688 V17X-E203X 0.389835808362557 A14X-V206X 0.387315000531306 Y7X-N124X 0.385133379433788 N111X-F131X 0.385031335138905 R15X-I194X 0.384942799213899 C232X-K252X 0.384643851775875 Y195X-A205X 0.382896805059131 K32X-E59X 0.381078181845863 K12X-Q196X 0.380837584505636 Y7X-L142X 0.380668740257653 Y22X-S234X 0.376532968118061 V129X-P136X 0.374364383324583 Y7X-Q154X 0.37221178772398 Q196X-L202X 0.370583855926203 N124X-A141X 0.369516592175819 L39X-T60X 0.368354403088059 S137X-F157X 0.36512204483968 N207X-K236X 0.362472706475151 V171X-T182X 0.360868619795221 K115X-G126X 0.35845213370685 T6X-L142X 0.358444110774773 A141X-M155X 0.358082567113939 Y153X-S167X 0.357689009046199 D30X-T42X 0.356883579336807 L180X-Y195X 0.356293686773387 Y7X-A170X 0.354925234540266 G244X-S260X 0.352800880036089 G23X-K28X 0.347327127291742 L150X-Y173X 0.346803590409108 E73X-T86X 0.346105118284922 V87X-T98X 0.341793599579377 F18X-C232X 0.337110408273873 Y7X-V143X 0.333541391826346 W75X-G82X 0.331174559881076 G11X-Q179X 0.327486465781572 A223X-K236X 0.325355836981869 A170X-G187X 0.325349484482806 G21X-A222X 0.323874313741116 V17X-S234X 0.322958379513431 T6X-L125X 0.322699362609896 Y7X-Q166X 0.322293012535216 A222X-S234X 0.321971272609498 T6X-G126X 0.315367249889902 N185X-F190X 0.314606949813109 L10X-A170X 0.31330162773538 K224X-S260X 0.313208214526003 L31X-S43X 0.311267947410462 T6X-A141X 0.31094506790666 L31X-F41X 0.310523952881956 L26X-S46X 0.309496519710606 A14X-Q196X 0.308601938923326 A8X-S13X 0.308115576408638 V17X-Y247X 0.305750737749528 L31X-S44X 0.302941766032716 L29X-S46X 0.302092056831464 E73X-E84X 0.300540467688387 Y195X-V206X 0.299251899917985 S57X-G82X 0.299124268767692 C127X-I138X 0.297535262778631 Y247X-L257X 0.296662480614801 Y195X-E203X 0.296607964513626 S101X-A112X 0.294877342402904 Y22X-Q249X 0.29393075746125 S44X-L58X 0.291562547364319 L202X-Y225X 0.291149700043812 V184X-E189X 0.286938891512403 T19X-T258X 0.28668580524222 N185X-G192X 0.284984116147149 G23X-L257X 0.282182070617801 Q166X-E189X 0.280210976367643 L150X-A170X 0.27892047845823 A205X-S234X 0.278441579276237 G126X-N156X 0.276405870485629 N76X-E84X 0.275470821220703 G220X-K236X 0.271701298681275 A170X-S193X 0.269734011461023 G140X-N156X 0.268803757829017 G172X-Q179X 0.267215291070217 G152X-N185X 0.267165695013037 T204X-A223X 0.266166512030817 Q179X-Y195X 0.266093287800182 G11X-L180X 0.265964877757008 A235X-I243X 0.263804351034626 Y7X-L125X 0.262426354996807 I243X-A261X 0.25921983847398 Y153X-H181X 0.257431368104716 K12X-N207X 0.254102076427369 R139X-F157X 0.253715541824812 L208X-G220X 0.251261432229878 G187X-W210X 0.25111390156776 Y118X-C127X 0.250151505593096 V143X-V171X 0.249546211482191 Q179X-E203X 0.249324317747134 S167X-N183X 0.247264533659461 K224X-C232X 0.246849045828869 V171X-V184X 0.243790707294881 L180X-G192X 0.243655409236377 A8X-R139X 0.242476741934747 K61X-E84X 0.242235554122165 Q154X-G187X 0.241947548159725 T60X-T86X 0.240104721864165 Y118X-D128X 0.239797199033086 Q196X-Q226X 0.238302306970414 G191X-N207X 0.238261929907723 F131X-S137X 0.238177895951531 L144X-G152X 0.236353605119585 I194X-N207X 0.233985743004304 R15X-A223X 0.233594651686116 A112X-S137X 0.233352754425137 F190X-L208X 0.233142940562766 L245X-A261X 0.232199804285162 T19X-L245X 0.231767628274521 G23X-L29X 0.231342407703882 G246X-T258X 0.231340539095737 A8X-R15X 0.230549596427643 V17X-V206X 0.230240651784832 L31X-G56X 0.228854820085951 K20X-A261X 0.22838009397085 R15X-Y195X 0.227541498122832 E73X-N111X 0.227117606249314 G140X-M155X 0.225029204422542 S57X-S102X 0.224988589685839 L10X-N183X 0.224911902337842 K61X-W75X 0.224522411343632 K236X-Y247X 0.224389371961108 S13X-S234X 0.224243511037321 S13X-A223X 0.223776249486432 E84X-K113X 0.222892985355971 Q249X-T258X 0.221911005055118 Y62X-K74X 0.221690420755562 Y22X-G244X 0.220978349021768 N168X-N183X 0.220854359388166 T250X-T258X 0.22016481355876 K20X-Y225X 0.220030285542093 Q154X-N185X 0.218045306380095 K12X-H181X 0.217795393166596 P5X-V143X 0.217339039838272 G246X-A261X 0.216785743734339 M155X-N183X 0.215953709334852 L144X-A151X 0.215944951799706 C232X-G254X 0.215374167943017 G23X-G45X 0.214940049410027 R15X-A205X 0.214862202161999 G23X-S234X 0.214739892570758 E189X-W210X 0.214431417198983 Y62X-L69X 0.213959304954483 T70X-T86X 0.21321498016175 N183X-G192X 0.210530118884888 T248X-G254X 0.210507004545664 A14X-Q179X 0.207566965726857 K20X-Q226X 0.207395939159007 T182X-G187X 0.206482967353795 T6X-G172X 0.206465517819273 E73X-F99X 0.203827097749155 G21X-T250X 0.203066782783305 L26X-T258X 0.202285702793594 Y153X-D186X 0.198360480794037 D9X-A170X 0.198002673415688 A112X-D128X 0.197690273678145 T33X-T72X 0.197172779463368 Y173X-N183X 0.197093669879068 T60X-L81X 0.193861169567942 A8X-G140X 0.19298010895217 I114X-F131X 0.190861488861248 T248X-L259X 0.190723782646936 Y7X-A141X 0.189913618207046 T42X-N76X 0.189880687870898 G11X-T19X 0.189826241112929 D100X-T116X 0.189616096545001 H181X-A205X 0.188197922247361 G220X-N239X 0.18712231374898 V171X-L180X 0.185401227692159 A141X-Q154X 0.184914404223584 E84X-K96X 0.184636217299171 L10X-G191X 0.183705583857671 D30X-T258X 0.18329554028423 G21X-T258X 0.182855837411489 T70X-D100X 0.182412861375332 G192X-A209X 0.18234796233005 L125X-G140X 0.182078160682142 A14X-I194X 0.181258998435791 I194X-T204X 0.181254394953771 S13X-Y173X 0.180827310809675 G117X-N124X 0.180340619850705 T116X-D128X 0.179423273748107 N239X-G244X 0.178634615487921 T116X-D130X 0.177712502650324 D16X-V206X 0.17727020595331 D9X-R139X 0.175902806787934 C232X-L245X 0.175108219931588 A170X-V184X 0.174406703342875 A222X-G246X 0.173757716155091 T19X-A261X 0.173278899992229 Q249X-L257X 0.173005147252244 V17X-G246X 0.172964025365221 D9X-G152X 0.171628989014544 G82X-D100X 0.170879191039495 E59X-T70X 0.17084718465012 R139X-D186X 0.170419145279217 Y7X-N185X 0.170040714641619 I85X-D100X 0.169026363858289 P5X-Q154X 0.168871241952264 F131X-N156X 0.168828955679522 T83X-S102X 0.168330257496158 R15X-R218X 0.168013440125956 T98X-D130X 0.166808935161215 A151X-G172X 0.166722942572069 L10X-G172X 0.166354750049042 K224X-T258X 0.166340555065276 G21X-A231X 0.165555275245081 Y7X-V184X 0.165044596394035 V227X-A235X 0.165042104744593 K28X-T248X 0.164378131781224 F18X-H181X 0.163755803263153 T42X-T70X 0.16251624149913 G126X-G152X 0.162249130044394 T6X-T182X 0.162097847652479 T182X-L208X 0.162060612290593 S102X-P136X 0.161694761256921 K12X-E203X 0.161116906890633 T70X-T116X 0.160755658267631 S193X-R218X 0.16059600195248 K252X-T258X 0.160472558154771 G11X-F18X 0.16043524908952 D30X-K74X 0.160219218810066 V143X-H181X 0.159617772477903 Y22X-V237X 0.15779711543371 K12X-G172X 0.157330224059752 D9X-T182X 0.15655253816053 C127X-M155X 0.156154976083076 T182X-W210X 0.155972516956364 M155X-N168X 0.155719563865778 E40X-T60X 0.154551252000879 K224X-G244X 0.153332864835915 D9X-A141X 0.153162907854211 G126X-L142X 0.153109475883509 T6X-D128X 0.152602927655937 T83X-K115X 0.152590057664375 A14X-E203X 0.152036181639687 K28X-T42X 0.151918407254042 K61X-D100X 0.151259739373842 R15X-Y22X 0.15053142426855 T86X-K96X 0.149985775928628 K12X-A170X 0.149786999513987 L180X-Q196X 0.149756483353671 K12X-G192X 0.149717977056585 Q226X-G246X 0.149077114482792 V17X-I221X 0.1489810391208 N156X-D186X 0.148715903539621 K61X-G82X 0.148232318860243 R15X-A209X 0.148097471078595 S13X-A222X 0.147938736370367 G172X-G191X 0.14695458970184 G152X-S167X 0.146822323034028 A141X-F157X 0.14667381845673 V17X-G244X 0.146278299075823 G117X-V143X 0.146220211249744 A205X-I221X 0.145452037738574 L97X-G117X 0.144825270333144 F18X-I221X 0.144726487051521 A170X-D186X 0.144568265324406 L26X-G56X 0.144132036816571 A235X-T258X 0.143847251971835 G11X-Q196X 0.143505597417505 L245X-S260X 0.143408628413718 D9X-N124X 0.142397993390328 Y7X-H181X 0.142279657053669 G21X-K28X 0.142017834423209 T19X-T248X 0.141905136641812 K236X-L245X 0.141735536850346 K12X-T204X 0.141453735997775 G21X-T248X 0.139212377107303 N111X-S137X 0.139208725063213 D128X-N156X 0.136281104244365 Y7X-Y173X 0.136069107410472 T42X-T83X 0.13590902399418 A235X-G246X 0.135824585775498 R139X-Q166X 0.135164448721622 C232X-Q249X 0.134668046493102 T60X-N76X 0.133718315111051 Y22X-Y225X 0.133653911560939 A170X-A209X 0.133643772146397 P5X-G126X 0.133199399288829 D130X-N156X 0.132827932121288 Y225X-F233X 0.132643332135919 A14X-A209X 0.132104887382019 T98X-K113X 0.131668416752373 G23X-L259X 0.13111942320797 A8X-N156X 0.128749804808908 Y7X-S13X 0.128728688668273 Y195X-Y225X 0.128647707616948 Y22X-L242X 0.128634250332277 S43X-E59X 0.12798840137452 T204X-N238X 0.127956453759887 G21X-E203X 0.127825669710782 T19X-I243X 0.127341798708943 A170X-Q179X 0.127076374932484 T98X-N111X 0.126966515666056 D30X-G56X 0.125277825187823 N76X-L81X 0.124724575396192 G21X-I27X 0.124338431884691 G244X-T258X 0.123771142459065 P5X-H181X 0.12278240196884 S13X-V206X 0.122614931058598 K20X-G244X 0.122498210616101 A223X-N238X 0.122264184815991 S43X-G56X 0.118463717890499 I221X-N239X 0.11800823656042 V143X-V184X 0.117984520274861 K20X-T204X 0.117884280598073 K32X-G45X 0.117749608479616 L26X-L257X 0.116928393169098 L10X-L125X 0.116436962076236 E59X-W75X 0.116193328250229 G23X-I243X 0.116114157047112 A14X-A235X 0.115940953432694 A231X-G254X 0.115938993354288 L39X-L58X 0.115914705219019 G11X-A209X 0.115720796785244 D16X-S193X 0.11535236423964 A222X-N239X 0.115147255184238 I27X-G45X 0.115064483899966 L125X-L150X 0.114519948672875 L202X-A235X 0.11442334210259 P5X-K12X 0.114415067478746 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