[Wed Aug 24 18:09:46 2016] >Input file: TM3V2YATD.pdb >TM segments: A48-64_A83-103_A122-140_A161-177_A208-226_A256-271_A297-311 MAIDEN score = -74.9097 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I60A-L92A 1.27088968048326 L136A-I217A 1.03893249864444 V132A-F210A 0.899529337219048 E62A-I309A 0.819717502251725 I59A-D91A 0.807242719618015 V126A-S171A 0.790988052440575 I59A-L92A 0.740879190420952 Y221A-I259A 0.718037915714295 T257A-Y311A 0.71332582319873 I60A-D91A 0.698319617404348 I84A-S134A 0.695235916579719 V264A-N303A 0.670069832313812 A139A-Y221A 0.669059395322892 G95A-V301A 0.663909679954806 C56A-Y98A 0.656995726292347 V126A-L172A 0.630157442052627 V126A-G176A 0.62634127971452 F83A-I138A 0.607375393898739 F83A-A137A 0.606217299844895 V261A-I310A 0.601551572556433 D91A-L128A 0.593407940333252 Y98A-V301A 0.570406577400083 F83A-S134A 0.565470332864198 A139A-L220A 0.559522334096943 S134A-I164A 0.559376850729644 S129A-L174A 0.554880267612122 I60A-V96A 0.553378281854905 L92A-S304A 0.551839888842943 S90A-L128A 0.551529134867477 C57A-E62A 0.54399750376456 N86A-I164A 0.534487123368377 T257A-I310A 0.526585771967539 F83A-L135A 0.523697936029445 L87A-L135A 0.51721779828492 S49A-L103A 0.485811344586253 I53A-L102A 0.478688128807361 A130A-W168A 0.473116342964147 L87A-A130A 0.472112365087353 I64A-L92A 0.467259379334046 S129A-I170A 0.465413839322996 C268A-L299A 0.455881176905983 S129A-G175A 0.453210401596687 V126A-W168A 0.45099568237363 A94A-S304A 0.445271093192472 G95A-S304A 0.444597775312673 C268A-L302A 0.424759958774267 A94A-V301A 0.40300883409165 F125A-G175A 0.401294657465944 A139A-I217A 0.394989235815716 L87A-S134A 0.389744926472092 W269A-L299A 0.387264295923046 I84A-I138A 0.375582352931918 W269A-N303A 0.37295606632983 A127A-A300A 0.366578839085215 W269A-A300A 0.364263734843714 C57A-V96A 0.364003747177518 L93A-A127A 0.354325047731829 E62A-G305A 0.35021764083994 Y98A-M124A 0.350192348632274 A137A-A166A 0.349199443452949 F52A-L102A 0.344255577508196 A94A-S123A 0.342305833904753 F125A-L174A 0.34202454725669 P271A-N303A 0.341283599138229 C167A-F210A 0.341139056474445 A97A-M124A 0.339564031627272 F83A-I164A 0.33766627992164 I217A-R223A 0.337519288813999 A94A-L128A 0.335544781535284 A127A-S171A 0.334001711536481 A88A-N307A 0.331826128941298 D91A-A127A 0.32016693790143 T48A-C56A 0.320153636680091 L214A-I266A 0.319008254017302 A97A-S304A 0.316344339107359 S90A-P308A 0.315699983136098 M124A-W269A 0.310985439351881 A94A-M124A 0.31081600999841 L135A-L262A 0.303604968190481 V218A-R223A 0.299766914281787 S129A-F210A 0.29950287654153 N86A-P308A 0.29783923105405 F52A-T99A 0.297787631614327 V126A-L174A 0.295487382559761 C268A-A300A 0.294430020370068 V132A-L214A 0.294287015059673 M124A-N303A 0.2896585877985 S123A-G175A 0.286253313894407 C57A-I309A 0.285153902299811 L87A-G305A 0.285087855860958 S134A-C167A 0.284717591755341 C56A-T306A 0.277558753165418 G85A-A137A 0.276781593181962 W269A-T306A 0.273453829748172 C268A-I309A 0.270118807794692 M124A-W168A 0.268672574976515 E62A-G85A 0.267390428897625 S131A-N303A 0.263277452557334 S226A-K256A 0.259190432662126 S90A-G305A 0.259054020025607 Y221A-S226A 0.256543748778624 V132A-L213A 0.255471659384798 I259A-C268A 0.249665591290629 F265A-N303A 0.247848676280181 A88A-P308A 0.247305159423732 I60A-G95A 0.23846331791777 I53A-Y98A 0.238133396175191 I260A-I310A 0.236535009654615 G95A-L128A 0.23331302067832 I53A-V301A 0.231256017364514 T48A-L103A 0.230289805263671 V264A-I309A 0.229937308893851 W168A-G176A 0.229717704096629 L262A-P308A 0.228604405461914 C268A-N307A 0.227041141473324 C56A-V96A 0.226684790401074 L136A-L213A 0.225855413077967 I140A-V218A 0.224714864761503 T208A-W269A 0.223211994243238 Y98A-S123A 0.221774466202641 L89A-A94A 0.220991928220247 G95A-V298A 0.219095920649935 I138A-C167A 0.216435677191056 C56A-I64A 0.215780141935908 A139A-S263A 0.21515556476333 L87A-N307A 0.215102452392877 I140A-C222A 0.213920621145778 F125A-A300A 0.213633396687528 L262A-N307A 0.213404901334188 S131A-V261A 0.212079484875313 F133A-V209A 0.211976563004164 S131A-S216A 0.211481291673845 I260A-C268A 0.209784223378508 S134A-P308A 0.209722940692309 S263A-N307A 0.209559209520706 I59A-G305A 0.209009460225194 L93A-V126A 0.207580840958918 D91A-V132A 0.206929506241766 Y221A-S263A 0.20571484568119 I259A-T306A 0.205521687011057 Y98A-N303A 0.204782743751646 S131A-A300A 0.204663686046446 S263A-P308A 0.204210454285731 S123A-S304A 0.203042015607055 N63A-G305A 0.200429055832658 C57A-T99A 0.198755905071991 M124A-L299A 0.197739248315157 I64A-G85A 0.195853129284758 I64A-T306A 0.195690739151622 N63A-L302A 0.195003338669919 N63A-L93A 0.190371679505074 V51A-F58A 0.188111822784466 E62A-L92A 0.187637358174486 I140A-L220A 0.187501936186174 I64A-G95A 0.187414729204423 T99A-A127A 0.185717226831756 E62A-A94A 0.183705630922431 L89A-C167A 0.183005689915374 G305A-I310A 0.182624690988794 F58A-T306A 0.182382176971842 I60A-L93A 0.182202759919946 F58A-T99A 0.18194355621433 A267A-S304A 0.179940070428869 L89A-G305A 0.179015936963067 D91A-A300A 0.178081134855547 G95A-L299A 0.177864281923038 V126A-G175A 0.176821989445248 C57A-G95A 0.176670344317408 L135A-S304A 0.17612661067601 G95A-L297A 0.176010255832226 C57A-L93A 0.175661406506195 F52A-N303A 0.174180337786858 A94A-S171A 0.173675175970123 F133A-V169A 0.172282571177766 V261A-G305A 0.171717543833736 L215A-C222A 0.171623452205969 N101A-S304A 0.171320965087483 S131A-L174A 0.170949799948302 A130A-I173A 0.170812968096332 C57A-L302A 0.168836115055936 Y98A-G305A 0.168526055445811 N63A-L89A 0.167796607432378 A130A-V209A 0.167148073884097 A88A-Y311A 0.167057157211678 A139A-Y225A 0.166840056633547 S304A-Y311A 0.166577197213381 G85A-A130A 0.16657545420726 A94A-G176A 0.165549924975199 I59A-I310A 0.165073504110357 T211A-C268A 0.164134161466858 I259A-N307A 0.162909489023348 A94A-T306A 0.162900583802536 V218A-A267A 0.161880383558223 D91A-T99A 0.160626977566591 I138A-C222A 0.159514769343129 A127A-G176A 0.159469395329075 A94A-G122A 0.159368429088144 S90A-V301A 0.159128831164899 G95A-N307A 0.158324935326146 F133A-F210A 0.158302534787565 P271A-L302A 0.15826399252987 A97A-V298A 0.157773873840897 A137A-F161A 0.157035498239131 N63A-F83A 0.156763512921819 T257A-L262A 0.155944301203305 I84A-I309A 0.155073987132699 S134A-L162A 0.154722430038828 L128A-C167A 0.154084759181977 I140A-I224A 0.154035031433677 G95A-A300A 0.153592638523169 I84A-I259A 0.152958007829457 D91A-V261A 0.152703407108193 D91A-N101A 0.152370974654981 L136A-W168A 0.150930862118682 I55A-A100A 0.150356551698663 A137A-I259A 0.150231793053889 F83A-N307A 0.150146229798353 A267A-V298A 0.148891605296312 L89A-S171A 0.14883331515141 C56A-L297A 0.148249353541304 N86A-L135A 0.148238684234143 I170A-G175A 0.14705422641897 V126A-A166A 0.146493448053849 D91A-G305A 0.146274981386085 E62A-I84A 0.146038874853376 L61A-N307A 0.145771395749432 V51A-V96A 0.14574369669166 L135A-I217A 0.145420003547323 V132A-A270A 0.145031282129865 L87A-S171A 0.144861408085381 W269A-V301A 0.144829610343093 L93A-S171A 0.144631573971104 I55A-A97A 0.144594876274089 V132A-C268A 0.143662268666802 N63A-L92A 0.14266735560844 A100A-V301A 0.142366090315536 C57A-S304A 0.142049534080844 V209A-V218A 0.141461081359226 I53A-V96A 0.141386099889774 L213A-W269A 0.141309276681831 C56A-S90A 0.141042618348437 S134A-S263A 0.140403139949833 Y225A-K256A 0.140231193061625 F133A-F161A 0.139609909323069 N86A-L128A 0.139336877606963 V264A-W269A 0.139012730234021 S263A-N303A 0.138712993497599 A137A-S216A 0.138322395451937 V264A-I310A 0.137982142032979 I266A-L297A 0.137910945782294 V126A-V301A 0.137890228982947 F125A-S171A 0.13774181690512 V132A-A166A 0.137302307392811 S263A-T306A 0.136952995162812 Y98A-L103A 0.136895903793507 I64A-N86A 0.136728934248088 V261A-V301A 0.136678138287791 L92A-I309A 0.136003704693884 S131A-V264A 0.135778275957764 I138A-K256A 0.135357555071437 S90A-V169A 0.135160510770581 V132A-S263A 0.134720135151216 N63A-Y311A 0.134212929480727 C56A-L89A 0.132294232017628 M124A-L177A 0.131887731801104 N86A-A94A 0.130430577827205 V218A-K256A 0.130348271971754 C56A-G95A 0.129945159333252 L215A-W269A 0.129691661593694 E62A-N86A 0.129432038529918 I217A-I259A 0.129412159819724 L93A-V301A 0.129285510728753 I84A-T306A 0.128569267701532 L89A-M124A 0.128300908538986 I59A-G95A 0.128104240399074 I55A-N303A 0.127903589945408 T99A-S123A 0.126868327651473 G95A-T306A 0.126828721412429 L135A-W168A 0.126096065905059 S123A-I173A 0.125927907772832 K256A-V264A 0.125618220780395 F58A-N307A 0.125528422436592 V50A-L103A 0.125275967370959 D91A-S123A 0.125262982100709 A100A-G122A 0.125188132830079 L87A-I309A 0.125051641284325 S131A-V169A 0.124989332972709 T208A-I266A 0.124912539657673 L302A-P308A 0.12487459652968 I60A-G85A 0.123807491664759 I64A-L89A 0.12376090972865 L213A-V218A 0.123038833013579 I53A-G305A 0.122822258393724 V132A-A300A 0.122791641065771 S90A-F265A 0.122271863669084 S129A-T211A 0.121685342830997 F125A-S304A 0.121240993457646 I260A-N307A 0.12065706509437 L93A-N303A 0.120353112444139 G85A-F161A 0.120026272029384 L174A-L213A 0.119566558058762 F83A-V132A 0.119508413512983 L93A-G305A 0.119109940643708 I64A-Y311A 0.118313418678036 S134A-V261A 0.118047494499535 S123A-W168A 0.117739092132659 V264A-G305A 0.116881621020225 S134A-V258A 0.116671684463922 N86A-S129A 0.116666130810967 S129A-S216A 0.116094861555719 G85A-S131A 0.115659794286453 A94A-V132A 0.115569394740854 L102A-L297A 0.115325064913748 L135A-S263A 0.115190246100999 L102A-L302A 0.11484842620056 S131A-L213A 0.114818335303278 S129A-W269A 0.114187481694518 L93A-L302A 0.113939514209977 G85A-V258A 0.113459630420772 A137A-L213A 0.113432490182132 L61A-S304A 0.113418436556226 S49A-V301A 0.113044115722723 L128A-L299A 0.113009514836845 L92A-L302A 0.11267239666939 V132A-I170A 0.112613610353529 S131A-W269A 0.11209833746893 L136A-L212A 0.111960244948665 S129A-A166A 0.111366649049429 S134A-L214A 0.111344231516587 K256A-V261A 0.111038736948429 I170A-L213A 0.110813439797298 Y98A-S129A 0.110044256575148 S134A-Y221A 0.110044256575148 I59A-S90A 0.109932725900116 A270A-V298A 0.109473699400076 A97A-G175A 0.109397738043042 F265A-A300A 0.108949486319908 L87A-L262A 0.108808581123354 S90A-S134A 0.108686985938444 S131A-V301A 0.108390916508487 V51A-G95A 0.108374065936864 V218A-S263A 0.10800514808181 L214A-A300A 0.107951127891202 A88A-N303A 0.107398680956825 S90A-T306A 0.106357829395496 S131A-T306A 0.10619210915368 A94A-G175A 0.106183373165904 S131A-T211A 0.105574508524209 A88A-S129A 0.105381042406817 L87A-S304A 0.105375744928386 S263A-A300A 0.105008672624596 S129A-L214A 0.104859466234387 L135A-L212A 0.104822130769884 I84A-A139A 0.104491056905387 L215A-S263A 0.104305770974969 S131A-A267A 0.1040025135177 I55A-L103A 0.103855178966317 A127A-V298A 0.103842536995463 I84A-L135A 0.103680562106569 V132A-V169A 0.10354155109972 A94A-N307A 0.103528380691659 V96A-L128A 0.102751263131212 A270A-S304A 0.102125988526582 A130A-L177A 0.102004941094358 C57A-L103A 0.100557466586029 I59A-A300A 0.100342012415129 L89A-P308A 0.100026402296541 L93A-Y98A 0.0996123764029315 L87A-S165A 0.0994342977061722 A97A-S171A 0.0994015124096581 M124A-V132A 0.0993484947025784 V132A-V258A 0.0992111392204427 A267A-L302A 0.0991683413125024 L102A-S123A 0.0987003832033304 F210A-C268A 0.0986475359743615 E62A-T99A 0.098119857901048 L174A-L212A 0.0979385854398656 I55A-A300A 0.0978404764817178 V132A-L163A 0.0976216129237493 A127A-N303A 0.0976134163629019 L128A-V298A 0.09746454397078 A88A-S165A 0.0972833025831576 A270A-V301A 0.0962168211979528 L136A-V209A 0.0961020877469814 S129A-L212A 0.0957338998076089 I84A-I140A 0.0957124023764795 L92A-S134A 0.0956941128311186 F58A-G305A 0.0956222728013696 S131A-N307A 0.0955593359015534 C57A-D91A 0.0951601343149517 S129A-A137A 0.094517312837878 L54A-V301A 0.0945080406994356 Y225A-I260A 0.0944563638041371 A267A-L297A 0.0944136786911822 V126A-S134A 0.0938470852302693 I53A-L93A 0.0938208578144524 L213A-I259A 0.0938017842234385 L215A-I260A 0.0938017842234385 L61A-I310A 0.0938017842234385 I138A-L162A 0.0938017842234385 L213A-L262A 0.093765172841582 A139A-F210A 0.0937544812499098 L93A-L172A 0.0936337465077117 L220A-I266A 0.0930079675750501 L135A-L215A 0.0929472268912948 L87A-L302A 0.0926278206846982 I84A-G305A 0.0924347919627011 S129A-A270A 0.0922812744517918 A130A-L214A 0.0916836912933701 A130A-G176A 0.0916499757151281 A137A-V169A 0.0915747640941662 I217A-V261A 0.0915464581319112 I138A-V258A 0.0912810590559285 L128A-L262A 0.0907989925618238 I64A-A88A 0.0902910185524672 V209A-I217A 0.0899855253589933 L93A-I164A 0.0899715769504581 V264A-T306A 0.0898132939654497 A127A-C167A 0.0897731041290715 S129A-A300A 0.0896558381025008 I266A-V301A 0.0895743780784706 S90A-A137A 0.0893844350578203 N86A-V132A 0.0893601080148413 A139A-C222A 0.0893462779084681 L102A-L299A 0.089199020423536 C222A-V258A 0.0891331565710374 L92A-A100A 0.0888442729494332 S123A-L177A 0.0887593994912363 A130A-L163A 0.0886589332242891 L172A-L177A 0.0883951390814728 I84A-L128A 0.0883693222424765 L128A-S171A 0.0883277173575312 F52A-A100A 0.0879424306003997 N63A-L87A 0.0879128034640058 A97A-L302A 0.0877051917875664 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