[Wed Aug 24 18:44:34 2016] >Input file: TM4A97CGD.pdb >TM segments: A196-213_A219-236_A254-272_C814-829_C833-850_C868-885_C903-919_B505-520_B527-543_B560-577_E1427-1443_E1446-1463_E1483-1501_E1514-1532_D1121-1136_D1139-1156_D1177-1194_D1207-1223 MAIDEN score = -180.2734 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- I271A-W1442E 1.19089128758992 W1135D-I1499E 1.16679009289208 F1134D-C1211D 1.0857553359385 F827C-C904C 1.07612619504786 I1192D-W828C 0.995673647490186 R221A-I270A 0.958734548139299 W196A-I564B 0.919445841374227 F1441E-C1518E 0.847854627659739 V1154D-Y849C 0.818119433356034 L1497E-Q1516E 0.815655704688247 T227A-F533B 0.800586000371154 T1148D-F1454E 0.798387501287302 L222A-I267A 0.774396684623228 F1147D-T841C 0.767111052213805 S823C-I877C 0.748057502923179 V826C-E834C 0.74709764028072 S1130D-I1184D 0.726684745761329 S516B-I570B 0.725933748840937 V519B-E527B 0.725589516874779 F840C-T534B 0.716771879560147 I1127D-G1180D 0.715217757963375 V233A-Y1463E 0.710396486225816 I513B-G566B 0.706906704795933 F226A-T1455E 0.706552061598449 L1190D-Q1209D 0.702751538488808 I206A-G259A 0.698725725423238 S1453E-I1495E 0.696580645387203 Y1156D-V1461E 0.692108535815314 L1125D-Y1488E 0.688292341422801 Y260A-L1432E 0.687137140089381 V1153D-G1180D 0.680941887702813 V1460E-G1487E 0.678292651531023 V1133D-E1141D 0.678227534715933 L537B-Y542B 0.670727182192322 S1146D-I1188D 0.663229162009738 V847C-Y542B 0.655983890668979 V846C-G873C 0.652989791612767 T227A-T1455E 0.651488801553038 V539B-G566B 0.646642646693484 S225A-I267A 0.628654912797231 F1526E-C1531E 0.628241495124912 W1438E-F1522E 0.623951787803879 I820C-G873C 0.623365092955053 F912C-C917C 0.620656222965408 L1450E-I1495E 0.610344438052411 L529B-I574B 0.608794429079746 Y1181D-L818C 0.604193286201528 F1185D-A822C 0.604059489709592 A1129D-F1492E 0.598157581341471 A1194D-I1208D 0.594500145239716 Y874C-L511B 0.592795361916684 S209A-I263A 0.591279390140641 R1449E-I1498E 0.588244819612131 F878C-A515B 0.586937699101555 Y235A-V540B 0.586782654153076 S532B-I574B 0.581370279140487 S209A-L228A 0.579995007341514 S823C-L842C 0.573295041492696 V232A-Y260A 0.570510295672729 I1434E-G1487E 0.569526061627124 R835C-I885C 0.569319065795077 S839C-I881C 0.568591886354811 V539B-Y567B 0.564505985800221 V846C-Y874C 0.563057148431322 S1437E-I1491E 0.554664500225241 F226A-L1456E 0.553932566875993 F840C-L535B 0.540797126483799 V1460E-Y1488E 0.540575749002541 V846C-I870C 0.540056090420193 R1142D-I1191D 0.539560479593372 I513B-S569B 0.536904547994167 S1130D-L1149D 0.535258763339488 S1437E-L1456E 0.533985617863467 S516B-L535B 0.533902641746832 T841C-T534B 0.532983002193296 V1153D-I1177D 0.5281900994462 F264A-L1432E 0.526303555456978 L836C-I881C 0.525217933087499 S516B-S569B 0.521548315289542 Y1181D-A1186D 0.519850776696136 S823C-S876C 0.518214222196748 Q223A-L228A 0.51566340342325 V212A-E220A 0.51493267071702 L204A-F571B 0.514535866678859 F1215D-L1220D 0.514022276538393 F878C-L511B 0.512906810684168 F1147D-L842C 0.509636190972572 F1185D-L818C 0.505877874200379 L1125D-F1492E 0.503920333718565 A208A-F571B 0.501821763271613 F264A-A1436E 0.498967661586269 V232A-G259A 0.494752660288143 F1441E-R1449E 0.49405061576855 F827C-R903C 0.493372646849576 F1441E-R1517E 0.491161430766816 I820C-S876C 0.490711432129601 F1134D-R1210D 0.488970953132565 T538B-A543B 0.483509487766677 L204A-Y567B 0.482931987151932 R528B-I577B 0.480384347744569 R1142D-I1192D 0.478856849451139 L1143D-I1188D 0.478589204533444 R835C-I884C 0.478126282453991 F1134D-R1142D 0.475147818451405 L222A-I270A 0.469051849235119 L509B-M562B 0.468932305537761 F213A-R221A 0.461773839393703 L1497E-I1515E 0.460468620875444 F827C-R835C 0.457851765115667 F1446E-I1498E 0.457818821607004 V1460E-I1484E 0.451982895336354 S823C-S839C 0.450815963439874 M229A-I263A 0.445543469029248 T1148D-T841C 0.44297669650112 F1139D-I1191D 0.442688525707826 T231A-A236A 0.442048266711172 E834C-T531B 0.438421616432895 I1495E-F1500E 0.436041085333154 Q837C-T531B 0.433130658132141 S262A-I267A 0.431710609911195 L1207D-R1212D 0.428131350311399 D560B-A565B 0.427624108134447 L829C-E834C 0.426700563766339 V232A-I256A 0.424703586949139 Y567B-A572B 0.424252248040542 I1188D-F1193D 0.423268168139047 V1153D-Y1181D 0.422031298520691 F520B-R528B 0.421977338185116 L228A-I263A 0.41853831323655 I1427E-M1483E 0.418399333500029 T224A-E1448E 0.417032382242882 V1133D-R1212D 0.415826415320956 T231A-L537B 0.412306341553717 V1440E-T1452E 0.412306011989764 S1183D-I1188D 0.412096781559447 R1212D-L1217D 0.410049923604172 V1440E-R1519E 0.408971097465593 V826C-R905C 0.408144529420943 L1456E-I1491E 0.407924522809751 Y874C-A879C 0.405616650437594 T838C-E527B 0.405209727804748 V539B-I563B 0.402345266029979 I820C-M869C 0.399643236384746 L230A-Y235A 0.398476721541275 Y260A-A265A 0.39101890745866 L1149D-F1454E 0.390609925000714 Q1451E-L1456E 0.390608634585895 V1440E-E1448E 0.38944864667274 W517B-M536B 0.38592602139076 W1438E-M1457E 0.384935554400172 A879C-A514B 0.378684020029237 A234A-A848C 0.377776855320653 A1435E-A1486E 0.376422408280435 E220A-T1452E 0.374285788391413 A1155D-L1458E 0.373471218353034 L529B-I577B 0.373313051463585 A879C-A907C 0.372839848777174 S876C-I881C 0.37184715929202 F1446E-Q1451E 0.371236573506916 L1151D-Y1156D 0.370741363343966 A880C-A907C 0.366704734258072 L1527E-V1532E 0.364940732030111 P1429E-S1437E 0.364684836140142 A236A-A543B 0.36461717462315 L844C-Y849C 0.364393768080406 A1493E-A1521E 0.36361111794286 P508B-S516B 0.362521759885939 P815C-S823C 0.36201726502994 A514B-A565B 0.357880457148309 L818C-S823C 0.357376685366236 L1143D-I1191D 0.356955312104969 P201A-S209A 0.356419150224162 I257A-S262A 0.356131015253593 W824C-F908C 0.354947371492051 W1131D-M1150D 0.354632812990344 V826C-T838C 0.354042891627772 A1501E-Q1516E 0.349283144106955 F1185D-C1211D 0.346433630179871 A848C-A541B 0.344679920213821 V519B-T531B 0.344544917990851 G1431E-Y1488E 0.343523683918106 E1141D-T838C 0.343020007447191 Q868C-G873C 0.341567235794924 V233A-Y542B 0.341510761959754 L816C-M869C 0.338133463473854 S225A-A266A 0.337485556163626 A1494E-A1521E 0.336402959604678 V1133D-T1145D 0.3339452551798 M536B-Y567B 0.33212435906197 Q530B-L535B 0.331881993766787 A879C-G911C 0.330340217869912 Y849C-V539B 0.330090627376387 F840C-T531B 0.32991505354336 A265A-A1435E 0.329895497000575 A1155D-A850C 0.329815097475914 G1182D-A822C 0.328789007778278 E1448E-Q1516E 0.32809860957616 L844C-A541B 0.327763234197951 S1447E-T1452E 0.325988236959644 I257A-P1429E 0.32536019477161 A848C-A543B 0.324337739274544 S1146D-L1151D 0.323902695584278 L1430E-M1483E 0.322431003077759 E834C-Q530B 0.322035075237702 I870C-C917C 0.321996256324479 L230A-A1462E 0.321623083590988 T1148D-T534B 0.321506193522931 V1440E-L1450E 0.320491835153746 L511B-S516B 0.320405041938002 L535B-I570B 0.320398791048827 S839C-L844C 0.318729240417194 E1141D-F1454E 0.318231050524918 S1490E-I1495E 0.317190520579594 F1139D-Q1144D 0.317181940358509 S1453E-A1494E 0.317024794070268 S197A-A565B 0.316881850828485 S209A-A258A 0.315799129496066 G1431E-G1530E 0.314586860759395 I513B-M562B 0.313115712261212 L1458E-Y1463E 0.313044112500379 S876C-P508B 0.312817541603018 G1180D-G1218D 0.312660687979256 S1130D-A1179D 0.312354964510961 T1145D-E834C 0.3122947655866 S516B-A565B 0.311517161680674 L1450E-I1498E 0.311097125801556 I1485E-C1531E 0.310839880433283 S197A-I564B 0.310539014838181 W210A-M229A 0.309603011724553 S823C-A872C 0.309565078954956 S1439E-R1519E 0.309451433133983 S1183D-P815C 0.30938977982412 A850C-I870C 0.308704736625822 V1133D-L1143D 0.307393052217887 F1147D-E834C 0.307364878397332 A207A-A258A 0.307108578320686 P1122D-S1130D 0.306639625918458 A1128D-A1179D 0.305713921633821 V539B-D560B 0.302274300566236 A208A-G568B 0.301936442962741 P201A-S569B 0.300250718103055 Q561B-G566B 0.299950092141044 L202A-A207A 0.299879327346583 L1151D-A848C 0.29911204722214 L1123D-A1128D 0.298502769666277 L202A-M255A 0.297733611201632 A234A-L537B 0.297557828217083 L1430E-A1435E 0.297242772111102 T841C-T1455E 0.296240017787949 S876C-A907C 0.296035459805578 F827C-R905C 0.295994855825077 A208A-I574B 0.295249160834851 L1125D-S1130D 0.294993870314872 E220A-F533B 0.293514573094428 I267A-F272A 0.292913965136346 A1129D-I1495E 0.292195937718374 R905C-L910C 0.291218358181365 L1443E-Q1516E 0.290895824291313 S219A-T224A 0.290300173438241 G1182D-G1223D 0.290001248921256 M1457E-Y1488E 0.289730957413811 P201A-T538B 0.287623316499735 T224A-E527B 0.287480850356058 W824C-M843C 0.287251262068451 L200A-G566B 0.286419077587903 A1186D-G1218D 0.285894356867619 L509B-A514B 0.284462787194589 T845C-P508B 0.284437255731854 L1432E-S1437E 0.283894848980235 W1131D-T1148D 0.283015956529435 V1154D-Y1463E 0.282179682092335 A234A-A1155D 0.281071975657737 L1496E-A1501E 0.28049826394083 L816C-A821C 0.279806937751531 V212A-T224A 0.27924666803025 L1189D-A1194D 0.279170620142048 G1180D-L814C 0.27825067085449 R1519E-L1524E 0.278055026003536 A236A-A541B 0.277957774926252 L1514E-R1519E 0.277887696840439 L204A-S209A 0.277750605189286 L1121D-G1487E 0.277639908460076 G873C-L507B 0.277318747104723 Y235A-V1460E 0.275486433170824 G259A-L1428E 0.275274120828223 V847C-T538B 0.273643340707174 L230A-T1459E 0.272876636344577 S532B-G566B 0.272537555587196 S1146D-A1187D 0.27227643339848 M843C-I877C 0.271970383597291 S1132D-R1212D 0.27107422001628 E1141D-T1452E 0.271052288359927 W517B-T534B 0.271049364410745 T845C-T538B 0.269873762293082 P1122D-S1490E 0.2682733406789 T1455E-T534B 0.268134657790053 L913C-V918C 0.266772601191919 A880C-S518B 0.266520718540356 A821C-A872C 0.266488454116815 L1190D-I1208D 0.265994800778415 F1134D-R1212D 0.26566427574603 I1485E-S1490E 0.265448647005408 R1449E-I1499E 0.264978148635249 A822C-R905C 0.264430811065924 F1441E-R1519E 0.263743586215524 G261A-A1435E 0.263673755600331 T227A-T841C 0.263578093779364 A1155D-A541B 0.263304353121006 A266A-S1439E 0.260962910827432 E1141D-Q1209D 0.260889529953334 G203A-Y260A 0.26055177852084 Y235A-V1461E 0.260367834872142 P815C-Q868C 0.259696504747445 Y1156D-V846C 0.257549111219461 S1146D-G1182D 0.257494150211249 A1194D-Q1209D 0.257371518638615 E527B-T534B 0.257309228816046 G261A-L1430E 0.256665881184507 A1494E-R1519E 0.25595781099253 Q868C-S876C 0.255657613604946 Y849C-P508B 0.255062945846855 M229A-Y260A 0.25475693911175 M1150D-I1184D 0.254447164311599 M1457E-I1491E 0.254276572207227 M229A-V1440E 0.25289147968734 E1448E-C1518E 0.252168963490072 L268A-S1439E 0.252025748013286 A1436E-R1519E 0.251383949352638 S1132D-A1494E 0.251080609456121 Q254A-S262A 0.250999011368657 W824C-T841C 0.249506711717995 M536B-I570B 0.247632455759236 Q561B-S569B 0.247457454557251 P1122D-T1459E 0.247374206703318 Q1451E-T1459E 0.247369590461848 A872C-G911C 0.246437744957412 Q223A-T1452E 0.245868744336783 M1150D-Y1181D 0.245287097902052 A1129D-R1212D 0.24478183337645 A1128D-G1489E 0.244476716202117 Y1156D-V847C 0.243990857647407 F1446E-C1518E 0.243726419212106 W1131D-C1211D 0.243700639232753 S825C-R905C 0.243517491286888 T1145D-L842C 0.242186465582369 S1130D-S1183D 0.24207950539062 W517B-G568B 0.241604274884513 E1141D-Q837C 0.241107612139855 T227A-T1148D 0.241023841649255 Q837C-F520B 0.240646192070509 S839C-G873C 0.240391062328312 Q1144D-F1185D 0.240112822952822 Q530B-T538B 0.239668380331274 L1450E-A1494E 0.239136729152596 S516B-S532B 0.238694681802999 G875C-A515B 0.238120081674142 W824C-G875C 0.238080408227608 G1487E-G1525E 0.237965546890468 F1215D-G1223D 0.237716838683718 Q837C-F878C 0.237452962696219 G873C-G911C 0.237029563244725 S1437E-A1486E 0.236698898742608 Q1144D-F827C 0.235641207308764 S225A-G259A 0.235557040067063 S211A-A573B 0.235287924251709 R1449E-M1457E 0.234251011729367 G1124D-G1487E 0.234226009456155 M536B-D560B 0.233899722162454 L230A-T538B 0.233329001301586 C1518E-P1523E 0.233279317386401 T224A-M229A 0.233047153869621 M843C-T538B 0.23278605469126 F1139D-A1187D 0.232776679907017 L1149D-I1184D 0.232608611590057 A236A-A258A 0.231469547026233 P1122D-L1456E 0.231424896668796 L1123D-G1489E 0.23128936264083 W1438E-G1525E 0.231156270262523 F1134D-A1194D 0.230881173116615 Y1488E-F1522E 0.23085784829372 F198A-Q561B 0.230188299872429 P508B-Q561B 0.230097169267649 S833C-S516B 0.229520109333226 S1130D-S1447E 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