[Wed Aug 24 17:40:47 2016] >Input file: TM4EEBATD.pdb >TM segments: A296-311_A329-344_C296-310_C329-344_B295-311_B329-344_E296-311_E328-344_D296-310_D329-344 MAIDEN score = -63.3850 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- A308A-I310E 1.91010301542867 I310B-A308C 1.41778696258305 I310D-A308E 0.986837272551826 F306B-T305C 0.883157969748271 T305A-F306E 0.874050308740814 I300D-M341D 0.748090521330496 F306D-T305E 0.746142917658427 I310B-T305C 0.743642486003476 F306A-F301B 0.735811084686761 T299A-I297B 0.703771474484684 M302A-F301B 0.700384318516972 T305D-I310C 0.698821164907421 I300A-M341A 0.687868247269241 M302B-A298C 0.684216777259962 M302D-A298E 0.6825050167123 A308D-I310C 0.673348756246748 M302D-I297E 0.654703381000351 T305D-T305C 0.651522169161517 V333A-A338A 0.641067348623463 V333D-A338D 0.640838702488242 F306B-F301C 0.622386438018785 F301D-M302C 0.604305960553798 F301A-F306E 0.597303824733925 V333E-A338E 0.595879671938548 P303A-F301B 0.594847819885598 I310A-T305B 0.585277316793023 T305A-I310E 0.584292466323085 T305D-T305E 0.584061831611933 F301A-M302E 0.583281665474238 V333C-A338C 0.583083120246147 P303D-F301E 0.581082153218754 F301D-P303C 0.572450919611551 P303B-F301C 0.567788927586459 F301D-F306C 0.543649673600999 F301A-P303E 0.539702556523727 M302D-F301E 0.538512575472789 M302B-F301C 0.536551261521812 A298D-M302C 0.534713130773051 M302A-A298B 0.533490572087603 F306D-A308E 0.525174192800048 F306D-F301E 0.524960969679753 L304D-F306C 0.522261948682026 I297A-M302E 0.51740746214945 V333B-A338B 0.513664044529149 Y311B-A308C 0.506377925997815 I297D-M302D 0.506176838828321 F306A-L304B 0.505759884656569 I297C-M302C 0.505612987732724 F306B-A308C 0.505591065878586 A298A-T299E 0.505347575733575 I297E-M302E 0.495210523715349 I296E-M341E 0.493464717617546 A308A-F306E 0.491307343803356 I297A-M302A 0.491030070135534 A308A-Y311E 0.485475101081611 L304B-M334B 0.469758499014993 I296C-M341C 0.464473752750425 T299D-A298E 0.451031017593658 I297A-P303E 0.446774782176272 I310B-G309C 0.444406604540706 L304A-F306E 0.436279431051303 I297D-M302C 0.435813108801499 F306B-L304C 0.435374433607717 F306A-A308B 0.416947499884111 I296B-M341B 0.405498647353888 T305A-T305B 0.399323041718425 F306D-L304E 0.399028957979873 M302D-I307D 0.397072617114927 A298A-M302E 0.394663902155608 I300E-A338E 0.3883924821807 I300A-M302E 0.385318251340467 I300B-A338B 0.384681092408442 I297A-T299E 0.370346140411693 T299B-A298C 0.367273177856199 M302E-I307E 0.367130798244827 I300C-A338C 0.364873372545518 I307A-M334A 0.362270126516337 I310D-G309E 0.36217433700746 T305D-F306C 0.359655753929887 T299D-I297E 0.358385625333312 Y311B-V330C 0.35688278465814 T299B-I297C 0.349874456511354 I310A-A308B 0.34921871469753 I310A-G309B 0.343377066724763 L304A-A338A 0.338239030214254 M302B-I307B 0.337167464667417 M302A-V336A 0.336433635386711 M302B-V333B 0.336433635386711 M302C-V336C 0.336433635386711 L304C-M334C 0.332924636937648 L304B-A338B 0.327536285005669 A298B-A298C 0.326246809030986 L304A-M334A 0.325018207057554 I310D-Y311E 0.323836692022243 M302A-I307A 0.317434692167701 G309D-I310C 0.313341968381498 G309A-I310E 0.311200647437269 A308E-G309C 0.304948350864843 M302A-I297B 0.303472869825558 I297B-M302B 0.303192724344297 L304D-A338D 0.298631719610859 I307B-T305C 0.298042827694429 P303D-T305C 0.29628281042002 P303A-T295B 0.296016782831969 I300A-A338A 0.295508064031026 A298A-A338A 0.294415194405691 I300C-M341C 0.292975283917448 L304E-M334E 0.289738392852765 Y311A-V330B 0.289691842052612 I297A-A298E 0.289389283850157 L304D-M334D 0.288383582543253 L304C-A338C 0.280098118541495 T299A-M341A 0.273636229822404 V333E-V339E 0.272458594464768 I307D-T305E 0.270824522533918 M302A-V333A 0.270600866370462 M302D-V333D 0.266737529697439 F306A-T305B 0.266280699388727 V330A-V336A 0.265435506626654 A298A-A298E 0.263361436181593 Y311E-P328E 0.261786679855901 Y311A-I310E 0.261516678361067 I307A-T305B 0.257669695180045 G309D-F301E 0.257552780482268 I300E-M334E 0.25561822599888 M302B-T305C 0.25524656035752 I310D-T305E 0.250867458343691 M302C-V333C 0.249658970364859 M302B-I297C 0.246108635432061 F301D-F306D 0.245961124827223 L304E-A338E 0.245110607308405 V330E-V336E 0.242557317623262 I300E-M341E 0.239012802476554 I296A-F301A 0.236882508249021 T299B-T305C 0.232803665619325 V330C-V336C 0.231984781454647 I300B-V336B 0.231184086668343 F301E-G309E 0.230141782551505 P303A-L331A 0.229639231525388 I300C-V336C 0.228941380631343 T305A-I307E 0.227175329622476 F301C-G335C 0.225670884057954 Y311E-V329E 0.225350365173199 F306D-A298E 0.2240035210623 P303A-T305B 0.22253680588427 T299E-M341E 0.221903885089663 P303E-Y311E 0.221851388687035 I300D-A338D 0.219922897584958 I307B-M334B 0.219146632059918 T305D-T299C 0.218949150185538 M302D-T305E 0.218847626489859 I296A-V339A 0.216066548160024 A298C-A338C 0.215343340534259 T305D-P303E 0.215092770370072 I300B-M341B 0.214610073893024 P303B-P303C 0.210578878722038 T299B-M341B 0.207810780997777 M302C-I307C 0.20739721813195 Y311A-G309C 0.206523505226528 A298B-I297C 0.206436039027466 I296A-T305A 0.206214028163745 M334C-V343C 0.206162405258448 T299D-I307D 0.205546306421423 I300A-V336A 0.204347107864281 I300D-V336D 0.203875962700661 I296B-V339B 0.203751858711626 A298A-F306A 0.203306112419844 P303A-Y311A 0.200450383478756 P303B-G335C 0.200279824675015 I297D-A298C 0.200022583047202 T305D-I300E 0.199276556429036 T305B-G335B 0.198281424461019 G309D-I307E 0.197679555244909 T299C-M341C 0.197215981394566 T305C-G335C 0.197091696792854 T305A-I307B 0.197059641331872 T305A-T299B 0.196951395003731 M302E-V333E 0.196255257795453 I310B-V330B 0.19600559742053 I296E-F301E 0.195719659314634 T305D-I307C 0.193768891468287 I296D-V343D 0.193389540346931 Y311A-F306B 0.193133702427323 P303D-G335E 0.190998207299641 A298B-T305C 0.190457651445428 I296C-M302C 0.188966507925723 T305D-G335D 0.187017330565046 T299D-T305E 0.185606667777075 Y311A-M334A 0.185162321008875 T305A-T299E 0.184909459246562 T305A-A298E 0.184637861905104 T305A-M302E 0.182243554432715 I310A-V330A 0.181988312433023 G309B-M334C 0.18197403876866 T295B-P303B 0.181596102194437 G309B-L331B 0.179692116532706 A308D-G309C 0.179233060747936 A298D-T299C 0.176841107746096 G309D-I310E 0.176646017103249 G309D-M334E 0.176419685196928 T299A-T305E 0.175101329829397 Y311E-M334E 0.174677171362006 Y311E-G309C 0.173933404452552 F301E-G335E 0.173464956958341 A298E-A338E 0.17275522144984 I300B-T299C 0.172069594598293 T305A-I300E 0.171466589892893 T299B-F306B 0.170861766076006 Y311A-G309D 0.170780677161376 A298D-A298E 0.170574431596547 A298D-T305E 0.170242329136629 I297A-T295B 0.169211514714235 I300D-I300E 0.167776140065937 I300A-I300E 0.165701224332424 I296D-I337D 0.164875366584448 I307B-I300C 0.164548602952052 I307A-I300B 0.163114094068616 G309C-M334C 0.163037621963903 G309B-A298C 0.161636831452177 T299D-T305C 0.161587811269887 A298D-F306C 0.161343711559947 L304D-G309E 0.160882057432457 I300A-T295B 0.160769252236193 F306E-F306C 0.160128322046601 I297D-A338D 0.159470346441767 I296C-V339C 0.158837874958508 P303D-P303E 0.158758956808247 I307D-I300E 0.158742248435108 T299A-T305B 0.157990794648045 I300B-I300C 0.157433076802976 I296C-I337C 0.157216272369674 T299B-L304B 0.156881687205932 F306B-A298C 0.156054066013414 T299D-L304D 0.155578446286968 G309E-T305C 0.154761644321499 I297D-I300C 0.154629713592007 I296B-A298C 0.154097484697098 T299B-I307B 0.154065623282357 Y311A-Y311B 0.153705570752942 I297D-M302B 0.15335374207746 I297A-I307E 0.153058542729451 G309E-L331E 0.151832208845702 T305A-T305E 0.151688680721398 F301D-G309D 0.151674471590701 T305E-F306C 0.151418181815258 M302B-I307C 0.150761526950411 I310B-A332C 0.150407754001742 I307D-A308C 0.150340575031229 I300E-I340E 0.149526976663265 A308A-I307B 0.149473979997296 G309A-L331B 0.149102014687354 Y311B-V329B 0.148722431824504 M302D-I307E 0.148588996610942 A308B-I337B 0.14858779511333 T299A-I307A 0.148013244620789 G309D-P303C 0.146970428990018 G309A-M334A 0.146852309573652 L331A-G309E 0.146063855961778 I300E-I337E 0.145877925285764 I296A-I337A 0.14587719470437 A308E-I310C 0.145806180798639 P303B-L331B 0.145749577109434 M302D-A298C 0.145747187086975 A308D-I310E 0.145293825673355 I296B-I337B 0.145033993415461 T305E-L331E 0.144965768304735 T299E-I307E 0.144917113692689 I310D-A332E 0.144839561260469 V329E-V339E 0.144530597603947 T305D-T299E 0.144166626797075 I307D-T305C 0.143814074731456 A298D-F306D 0.143279403893415 T295B-T305B 0.143229815392839 I296B-V342B 0.142870321478484 I297A-Y344A 0.142051515343014 G309B-M302C 0.142005437779237 M334D-V342D 0.141961203311435 A332A-I310E 0.141870280629148 I297D-Y344D 0.141743589731235 I297E-Y344E 0.141093671161993 T305A-G309C 0.140616986709806 T305D-P303C 0.14059527010882 L304B-G309B 0.140507259143344 I307E-I337E 0.140268644371804 T305A-F306B 0.139867587898188 I297D-A298E 0.139408520139181 I310B-I307C 0.139387002563438 A298D-M341D 0.139042827109771 F301B-G335B 0.138633975806266 I296D-I300C 0.138115541554559 I300D-I300C 0.137957607387654 T305B-T305C 0.137654983752327 I300D-T299E 0.137627001212296 T295B-I300B 0.136940129526405 I300B-L331B 0.136937887664402 A298A-M302B 0.136842428608145 P303A-P303E 0.135679164181308 I310D-V330D 0.134730061481804 Y311B-V333B 0.134589785988187 L331E-I340E 0.134308066000094 F301E-A338E 0.134216962156607 I300C-L331C 0.133960545696644 I296A-M302A 0.133703343434071 M302A-Y311A 0.13339290993746 Y311A-M334B 0.133297423505891 M302B-G309C 0.132992298215123 V330E-V339E 0.132405915167314 A308C-V329C 0.132355230663091 F301B-G309B 0.131782338486001 I296D-A338D 0.131388406705122 G309B-L331C 0.131385288618835 A308D-A338D 0.130774232534192 G309D-T305B 0.130505306794447 T305B-T299C 0.130168311866737 L304A-V336A 0.129716836844259 F301D-G335D 0.129658290162363 I300B-T305C 0.129334297510405 I300D-T305C 0.129153793029593 I300D-T305E 0.129113508188036 V330A-Y311E 0.128706460149369 I300A-L331A 0.126667308325729 M334A-G309E 0.12660414821058 T305A-I310D 0.126342846554067 A308A-V329A 0.126267790744024 L331E-V336E 0.126194729292092 A298D-I300C 0.125973124263641 I296A-I297B 0.125723509578532 A308B-F306C 0.125193898975095 M302A-G309E 0.124202340439173 I310E-G309C 0.124144518562481 A308D-P303C 0.124127978568981 I310B-G335C 0.123958655123833 G309D-I310B 0.123772272536176 A308E-I337E 0.123458224108459 F301A-A308E 0.123425050488964 G309B-I310E 0.1222755279893 I310A-I307B 0.122245145832946 I300D-I307C 0.12121441559584 A298B-M341B 0.120940627393302 F301C-G309C 0.120911665688975 T305B-L331B 0.120596337183587 I297A-I297E 0.120420467569796 P303B-A308C 0.120321185655513 T305A-F301E 0.120105570282194 P303B-A338C 0.120041916914155 G309D-M334D 0.119598740828642 L304B-A332B 0.119542020531238 I297A-M302D 0.119151962198219 G309A-M302B 0.118321475375295 I310B-L331B 0.118233065639518 A298E-M341E 0.118049546340166 T305B-I300C 0.117957900950769 A298E-V342E 0.117607804299683 I310E-V330E 0.117486508025818 I297B-I340B 0.11742344897919 A298A-M341A 0.117304842459613 P303A-A338B 0.117010945024078 M302B-Y311B 0.116729216653461 T305B-I310E 0.116562019750925 I297C-Y344C 0.115973978512699 I296A-I296B 0.115926031388992 I296D-I296C 0.115894258311094 G309A-T305D 0.115610891263538 T299D-F306D 0.114797167574673 I296A-V343A 0.11454979760723 F301A-G309A 0.114535899591615 I307D-I310E 0.1144806268024 G309D-V330E 0.114329048045146 L304E-V336E 0.114224618901524 T305D-I337D 0.11376343429423 P303E-V339E 0.113422718631461 L304D-L304E 0.113401325529242 G309A-F306C 0.11295928220502 A298B-A298E 0.112630797011014 G309E-M334E 0.112587507582159 M302D-G309E 0.11226198695122 T305C-I337C 0.111734808197972 I310D-L331D 0.111729485515437 A298B-F306B 0.111325709617175 T295B-V342B 0.111105259132743 F301C-V339C 0.110558606084816 T305D-V336D 0.110500183700768 L304D-V336D 0.1103289758699 T299D-V339D 0.110201539495183 T305C-V336C 0.110201214571615 A308A-Y311B 0.10990019148831 T305A-V339A 0.109445672153895 A308E-A332E 0.109382619726948 T305D-I300C 0.109273877086304 T299B-V339B 0.108881233880698 T305D-I310B 0.108711979185873 A308B-L304C 0.1072640538263 V336C-Y344C 0.106843325392822 A308D-G335D 0.106614873589729 G309E-A308C 0.106489302070727 G309A-A308D 0.106139779627945 A298C-M341C 0.106085107684317 F306A-M302B 0.105973385471221 A308C-G335C 0.105397302301488 I296C-V343C 0.105074110516675 A298D-V339D 0.104860649427354 L304C-V336C 0.104769931045965 A308E-G335E 0.104628660144663 A308C-A338C 0.104528691906189 A298D-V342D 0.104422987062458 M302A-F306E 0.104102533290901 T305E-V336E 0.103998284878189 I300A-A298B 0.10344315891323 I300E-V336E 0.102863053901347 G309A-M334B 0.102640861418475 T299A-F306A 0.102593778801485 G309B-F306E 0.102435910426139 I310A-G309D 0.102429612855281 I297B-M302E 0.102185605420884 T299E-F306E 0.101202685498937 V333A-V343A 0.101186142745017 A332E-I337E 0.100798197446212 T305B-A338B 0.100674508642564 F301D-A298B 0.10027804506674 F306A-Y311B 0.0998470206351989 I297E-I337E 0.0996596284499653 L304A-V339A 0.0996233625547163 A298A-F306D 0.0995499101696393 P303A-A308E 0.0992529425553831 I310A-G335B 0.0991640703784155 M302D-I300E 0.0990299446941894 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