[Wed Aug 24 18:01:43 2016] >Input file: TM4FC4ATD.pdb >TM segments: A27-52_A55-88_A101-132_A137-172_A176-213_A217-245_C25-52_C55-88_C101-131_C137-172_C176-211_C217-245_B25-52_B55-88_B101-131_B141-174_B176-211_B217-245_E27-52_E55-86_E101-132_E136-172_E176-213_E217-245_D27-52_D55-88_D101-132_D137-172_D176-211_D217-245 MAIDEN score = -370.2920 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C160E-E196E 10 C160D-E196D 10 C160C-Y195C 10 C160A-Y195A 10 C160D-Y195D 10 C160B-E196B 10 C160E-Y195E 10 C160C-E196C 10 P132A-H212A 10 C160B-Y195B 10 C160A-E196A 10 K156E-Y195E 3.7743517540938 A167B-I183B 2.25797860103972 A167C-I183C 2.13437336476485 A167E-I183E 2.0895560461883 A167A-I183A 1.67235111009643 I74A-M170E 1.60913553226466 M170A-I74B 1.60390444484841 I74C-M170B 1.57481233681272 C160E-F190E 1.48312322156749 M170D-I74E 1.47692811035108 I74D-M170C 1.45539683965928 C160C-F190C 1.44875981030914 C160A-F190A 1.38459527526583 M85B-V238B 1.36253955052587 L87B-M101B 1.34694177380489 A171D-K179D 1.29123474649466 L87C-M101C 1.27571714998317 A171E-K179E 1.25222713186109 I67C-W184C 1.23705034542973 I67B-W184B 1.22715386160421 I67E-W184E 1.22588862178007 A167D-I183D 1.22092558818549 I67D-W184D 1.22050421741534 L87A-M101A 1.21606514118226 T146D-Y217D 1.20952647674347 T146E-Y217E 1.20292129709184 H197B-N233B 1.2003066907644 A171A-K179A 1.19633232692259 I67A-W184A 1.17060343045262 A171B-K179B 1.14100692291057 T81B-C165B 1.13589083892422 I45A-M61A 1.1280540947639 H197A-N233A 1.12417114328072 I45E-M61E 1.12284716025022 H197C-N233C 1.11857973878888 T146A-Y217A 1.11383086722787 C160B-F190B 1.10906317679508 T81A-C165A 1.10826929848225 W162A-F240A 1.09996118719236 I191A-E196A 1.08319158306178 T81D-C165D 1.08263096883808 V152D-G126E 1.07926369850166 I191B-E196B 1.07845651308996 W125D-F155C 1.07634017756127 W125C-F155B 1.07072500888076 F155D-W125E 1.06664379897816 M170D-K179D 1.06334894339298 F155A-W125B 1.05985450925283 C160D-F190D 1.05549129074892 H197E-N233E 1.05079465656524 T81E-C165E 1.05029403903037 W162E-F240E 1.04919364463804 I45B-M61B 1.0455507112322 W162D-F240D 1.04332228078478 I45C-M61C 1.04288746434871 W162B-F240B 1.04161314351754 W162C-F240C 1.03997783475296 W125A-F155E 1.03743000644887 A171C-K179C 1.0351360635918 T146B-Y217B 1.03424098381926 I45D-M61D 1.03256345915861 Y123D-F155C 1.03211269353338 Y123A-F155E 1.03202840655243 T81C-C165C 1.01393756952251 F155A-Y123B 1.01374756828899 W125A-V152E 1.01370275048807 W125C-V152B 1.00949120201407 F155D-Y123E 1.00876459166426 W185A-I67B 1.00737431486495 Y123C-F155B 1.00373989159462 I67D-W185C 1.00006177870253 I191C-E196C 0.994892546293169 F154A-V229A 0.987875498038986 M170E-K179E 0.980433575757456 L87D-M101D 0.979010357802764 V152A-W125B 0.97841037431219 I191E-E196E 0.977287509181666 H197D-N233D 0.976582585043735 I191D-E196D 0.975095832683211 W125D-V152C 0.971962965668094 V152D-W125E 0.971589474952066 A32D-G115D 0.964630030665148 F65C-W184C 0.95040754032406 M170A-K179A 0.945219822300571 F65E-W184E 0.943258491866279 A36B-V119B 0.935916959640295 W185D-I67E 0.935122428122631 G62D-L188D 0.928304766801412 N161B-H197B 0.927549865060874 M170B-K179B 0.926223970844672 F80A-V199A 0.919521365166665 I67C-W185B 0.914161329941605 T146C-Y217C 0.914143136855049 F154B-W228B 0.903531648611276 F240C-Y245C 0.897533346441831 F154A-W228A 0.89478169519288 M85A-V238A 0.890618166578971 G62E-L188E 0.88043271794179 F154D-W228D 0.87966493626282 K156C-Y195C 0.879441972209095 K156D-Y195D 0.877757672972603 A63A-W184A 0.869983475561282 I67A-W185E 0.868965094613096 F154D-V229D 0.865484225073875 G62A-L188A 0.861560891562334 L69C-I183C 0.860123667164226 G62C-L188C 0.859798495908606 A63B-W184B 0.856604564565075 L69D-I183D 0.855675865475704 A63C-W184C 0.855537395519589 F80B-V199B 0.852513024011069 L153B-W228B 0.84981666137409 F154E-W228E 0.846857505685283 M170C-K179C 0.842791735266401 F240B-Y245B 0.842575367956337 L69B-I183B 0.841095669581799 T177C-A178B 0.840395576410843 F65B-W184B 0.839820027064762 N161C-H197C 0.833775755912692 A63D-W184D 0.831265348605017 A178A-T177B 0.829319610328686 F154E-V229E 0.828172711753087 A63E-W184E 0.82775064396847 L153A-W228A 0.824714936756423 L79D-W109D 0.822336786213175 L79E-W109E 0.822280873510421 T177A-A178E 0.821163593322034 A167E-K179E 0.81669903032747 I45D-F65D 0.814735211307324 L159C-E196C 0.81189653916456 L69E-I183E 0.811298570073891 I42C-L204C 0.810311310951831 I42E-L204E 0.810171651538107 L69A-I183A 0.810035844143845 L122A-F155E 0.809526638986017 L153E-W228E 0.808133027743256 L122D-F155C 0.802371771141405 K156A-Y195A 0.802088676811095 F154C-W228C 0.79934592620675 I42D-L204D 0.797067076845609 L79A-W109A 0.796244949452827 L153D-W228D 0.793023321690916 F65A-W184A 0.789497857980031 N161D-H197D 0.788851922182337 F65D-W184D 0.787112909621255 L79C-W109C 0.787008223885485 F240A-Y245A 0.782140841651232 K156B-Y195B 0.780495469982307 L153E-V229E 0.776060703116 G62B-L188B 0.773252057932211 M61E-I191E 0.769127436664741 I42A-L204A 0.767643414707395 M61B-I191B 0.764864849751941 A167D-K179D 0.763777578176795 I42B-L204B 0.762862828075597 F65C-L187C 0.762167352875222 L159D-E196D 0.762129398826677 C165D-G237D 0.760928285453613 S116E-T203E 0.75820911529461 G126D-V152C 0.75732504980021 A178D-T177E 0.754547724311749 L122C-F155B 0.752677731526313 S116C-T203C 0.750474156147727 C165E-G237E 0.748657257764608 L153C-W228C 0.748584653867797 S116D-T203D 0.747430160594948 S116A-T203A 0.747194709796555 F155A-L122B 0.746178062345862 V164B-L187B 0.744141133712765 L159B-E196B 0.74330340818209 L113B-V199B 0.742652932861415 L122C-K156B 0.74223333736881 V138C-L204C 0.739631480252154 F240D-Y245D 0.73914667312901 V164E-L187E 0.736784555000282 I172E-K179E 0.731722446058145 I172D-K179D 0.731144835780904 I42D-T203D 0.727506875340412 I42A-T203A 0.727243526182471 L51D-S55C 0.726206357706498 I42B-T203B 0.725565466806632 T177D-A178C 0.723384959896485 L131B-L207B 0.72279449024553 V138D-L204D 0.722096456358619 I172B-K179B 0.721792254271358 F155D-L122E 0.721753644889006 M61A-I191A 0.720707689143624 W162C-M241C 0.71811622635997 V164A-L187A 0.718101384877631 F80C-V199C 0.716831742730353 L113E-V199E 0.716413589846436 A167B-K179B 0.715639463526886 F46D-V138D 0.714386671629549 F154D-T230D 0.714171578304253 I172A-K179A 0.711290096730733 F46E-V138E 0.710169943655741 F46A-V138A 0.709626023567866 F46C-V138C 0.708658422499446 F240E-Y245E 0.708264099023411 F154B-V229B 0.707709192916169 I172C-K179C 0.707033203847897 A75C-R173B 0.706848401962619 F80D-V199D 0.706330937633286 F65E-L187E 0.706148131476939 F80B-L105B 0.704897683659449 I42E-T203E 0.703372007421114 S116B-T203B 0.703056411652918 L122D-K156C 0.702919664342541 V164D-L187D 0.700711658269846 L122A-K156E 0.700620195615159 L163E-F190E 0.700320185345627 V164C-F190C 0.69997387661984 F80D-L105D 0.699365102201181 L153A-V229A 0.69823892536273 L113C-M202C 0.697988746892507 M85C-V238C 0.694960804263308 K156D-L122E 0.693933472855219 L163A-F190A 0.692657131450753 N161E-H197E 0.692570748111196 C165C-G237C 0.690520718567543 L159A-E196A 0.689886406095258 K156B-V229B 0.689664082493672 L159D-Y123E 0.68935452143742 K156C-V229C 0.688704129131198 F65D-L187D 0.688364643849289 F65A-L187A 0.688309012113483 F65B-L187B 0.687550951188213 G126C-F155B 0.687378623134256 L159E-E196E 0.686292707082417 N161A-H197A 0.685361818746653 L113A-M202A 0.68457803336833 I45A-F65A 0.684196321462489 M61D-A192D 0.684176597308433 M61B-A192B 0.683753428719178 M61E-A192E 0.68372812679462 V29E-G111E 0.683652940443346 Y123C-L159B 0.683361502445427 Y37E-L69E 0.682831795301408 M61A-A192A 0.682700137764979 L79B-W109B 0.682241405696937 Y37D-L69D 0.682170249261921 M61C-A192C 0.681778215848389 L159A-Y123B 0.680204288392706 F154C-V229C 0.678940979150993 Y37A-L69A 0.678923698203827 Y123A-L159E 0.677378500545908 L163B-F190B 0.677121788708416 V29D-G111D 0.676304410338537 Y123D-L159C 0.676162638140482 A36E-F118E 0.676017490165505 L40C-V119C 0.675670492799817 K156A-L122B 0.674253948667653 A32E-L113E 0.673049694271809 A32C-L113C 0.672582015787807 F154A-L231A 0.671348343555141 A32B-L113B 0.671157239257689 L40C-A68C 0.671144617961283 A192D-L48E 0.6708836110993 F46A-I137A 0.670840102182549 L48D-A192C 0.670825518724446 F46D-I137D 0.670385396433694 K145E-L153E 0.670279833630548 F46E-I137E 0.669846934618527 F154D-L231D 0.669100153414266 F46C-I137C 0.667478635404444 I42D-F65D 0.667391711108387 L48D-L59C 0.667063569417899 L48C-L59B 0.666569943549136 I42B-F65B 0.666009746610291 F80A-W109A 0.665957116400415 L59A-L48B 0.663778828551983 A75D-W169C 0.66347085809351 I42E-F65E 0.661585532934562 L48A-L59E 0.661109013142113 L59D-L48E 0.658312939714909 A36C-F118C 0.658039153848275 I42A-F65A 0.657097459905851 W162B-M241B 0.655956459807978 W169A-A75B 0.65498199101321 L131C-L207C 0.65456420131027 A75C-W169B 0.654533227943585 L40D-A68D 0.653769136652008 V138A-L204A 0.653258070286464 A75A-W169E 0.653161550196775 M202C-L226C 0.652496659152179 A32E-E78E 0.651603807194972 L166A-M241A 0.650528445638769 A75C-L166B 0.649546321688785 W169D-A75E 0.649390958235169 F80E-V199E 0.649347659843848 V29C-G111C 0.648728782485057 L163D-F190D 0.648185609746542 A36B-F118B 0.647921667209234 L113B-M202B 0.64626152404436 A148C-N225C 0.646254137192383 A148E-N225E 0.646239004845605 A150A-N225A 0.646176019200173 A167A-K179A 0.643372765967355 L113D-M202D 0.64195928183233 A148A-N225A 0.639981671476433 A150C-N225C 0.638850691616121 L166D-M241D 0.638551584063654 A148B-N225B 0.635826334515735 A167E-W185E 0.633684220195478 F205E-I222E 0.633170806076373 L131E-L207E 0.633130802065206 A148D-N225D 0.632862319560722 M61C-I191C 0.631281765793963 A167D-W185D 0.630863752181647 A36D-F118D 0.630656990392239 A150D-N225D 0.629662100328091 F80A-N112A 0.629097881768031 M202E-L226E 0.62726510417874 F86D-G242D 0.627143689360306 A36A-F118A 0.626209379320422 V164A-F190A 0.625763012139151 F86E-G242E 0.625754591337123 G62E-F190E 0.624743225514015 L163C-F190C 0.624061750392165 A36D-V117D 0.623852972856453 G62A-F190A 0.623431634159983 G62C-F190C 0.623028400679041 Y37C-L69C 0.622918092901244 K156E-V229E 0.622293884574004 F154B-T230B 0.62185113333665 L40B-A68B 0.621542201476733 A36B-V117B 0.62094356064507 L131E-H212E 0.620824131432092 G126A-F154E 0.620715403246496 F80B-N112B 0.620342683231744 Y123C-K156B 0.620209135474774 V238C-L243C 0.620140575945537 F86C-G242C 0.619930265888803 K156D-V229D 0.619401133232603 L48C-R57C 0.619294824463684 F154D-G126E 0.618789344858837 F80B-W109B 0.617667194275419 F46E-M61E 0.617274149857515 L113E-M202E 0.616824014784331 L44D-S193C 0.616184450628843 F80C-L105C 0.615511952595151 F154A-G126B 0.614274653501623 L40D-V119D 0.613754604035289 G62D-F190D 0.613684951615157 G126C-F154B 0.612737283652796 F86B-G242B 0.612593782123063 F86A-G242A 0.612495195975575 Y37B-L69B 0.612006514870401 L131A-H212A 0.611486907334797 V238B-L243B 0.611471691638177 L48E-R57E 0.610962390346695 L48A-R57A 0.609637707663827 V238A-L243A 0.609380564887183 H139E-H212E 0.609038215235392 K145A-I222A 0.608741302904015 V29A-G111A 0.608153846256053 L48D-R57D 0.607795993477634 V38C-L69C 0.607679154396553 L48B-R57B 0.607664793750931 F154A-T230A 0.60674144304471 K156A-Y123B 0.606102896410371 G35D-V114D 0.605340996363343 A150B-N225B 0.605151679706302 A32D-L113D 0.602189371006005 L163D-C186D 0.600911062281341 C165A-G237A 0.600169885256612 L113A-V199A 0.59868147348708 K145B-L153B 0.597498995035579 A150E-N225E 0.596818121968339 V164C-L187C 0.595883579385715 K145D-I222D 0.595526059349892 W109B-V199B 0.594006042840974 W162D-M241D 0.592825563214971 V164D-F190D 0.59233106516341 H83C-L105C 0.592171558637195 K156D-Y123E 0.590390719253511 W162E-M241E 0.58907466846067 L131E-S136E 0.587683981644506 A167B-W185B 0.585648205612939 G62B-F190B 0.584413834532863 L166C-M241C 0.584294781632932 S124C-L131C 0.583416361165974 H83A-L105A 0.582288392404053 R57A-G62A 0.581673898137275 R57E-G62E 0.581438275545071 K156A-V229A 0.581242528435763 S124D-L131D 0.580534261051817 F80D-N112D 0.580462653545858 A36A-V117A 0.579848113837829 K145C-L153C 0.579739432705021 L40A-A68A 0.579730502721251 A167C-W185C 0.579253226010268 F205C-I222C 0.578885206663212 A32B-E78B 0.578178785091284 K145C-I222C 0.577400633831116 F80D-W109D 0.57679385356517 L166B-M241B 0.576477754659603 A120C-F210C 0.574925666227251 A36A-N112A 0.574863124145346 V164E-F190E 0.573915231997775 V164B-F190B 0.573801727586055 L159C-S236C 0.573028939064402 L153D-V229D 0.571759647921678 V138E-L207E 0.571561448916809 I45B-F65B 0.571136675750461 G35E-V114E 0.570244432009924 F154E-L231E 0.570085503563583 H139E-H213E 0.569899994616226 G82C-C165C 0.569393731226336 A167A-W185A 0.568793267296659 N161C-G237C 0.568388031448638 L131D-S208D 0.568132622432016 R57D-I191D 0.567797353023362 W109A-V199A 0.567453053295536 S31D-E78D 0.566386908947828 A171C-I183C 0.564382941958756 A120A-F210A 0.562989654449997 S31E-E78E 0.562884130460019 S124E-L131E 0.562764277361965 S124A-L207A 0.561757731317529 S124B-L131B 0.561489215627331 S31A-E78A 0.561362644660874 A32D-E78D 0.56126098409505 F80E-W109E 0.559777126379553 F205B-I222B 0.559427235360152 I74C-R173B 0.559185286759746 T88B-M101B 0.558779611798307 H197E-T230E 0.558011322621829 N161D-G237D 0.557460637285005 N161A-G237A 0.556333501787114 F80C-W109C 0.556305048804271 L159A-S236A 0.555541171870831 T88C-M101C 0.554753620361257 C165B-G237B 0.554597094017348 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