[Wed Aug 24 17:59:42 2016] >Input file: TM4HZUATD.pdb >TM segments: T26-39_T41-58_T60-81_T111-127_T248-264 MAIDEN score = -48.0725 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I55T-I60T 1.10879263553723 I69T-I121T 1.07325542001507 V34T-F257T 0.977840623050111 V80T-I113T 0.891073184364956 V80T-F114T 0.869648433416723 F64T-T125T 0.795319454135809 S43T-I260T 0.779195924467063 W42T-F263T 0.727735757091748 I46T-G256T 0.711441172648819 V35T-R116T 0.700242855146724 I46T-I260T 0.697912610247327 V80T-G111T 0.642459534638639 A38T-Y44T 0.627047009447239 I69T-M122T 0.616553298182959 F50T-G256T 0.61474171334075 F65T-M122T 0.585817379321524 I76T-R116T 0.570986830905217 N39T-Y44T 0.569852069599439 A38T-F263T 0.569144363216704 I76T-F114T 0.553708026989487 F65T-T125T 0.548443218654665 T51T-M123T 0.538910788876353 S28T-L127T 0.520280657996535 I76T-F117T 0.511630535721435 S43T-R264T 0.507664132117956 F65T-L126T 0.502226254661458 I69T-L118T 0.479187808777724 I55T-L126T 0.478576591598239 R70T-I76T 0.425356896902118 L37T-R264T 0.404888460619363 I60T-F65T 0.400627627520117 L62T-K67T 0.376493461431727 G68T-I121T 0.372922122894904 I41T-R264T 0.362181009669646 C30T-F50T 0.360432115124212 Y44T-V258T 0.356973592672158 A38T-L261T 0.342850167493829 T51T-L119T 0.340245536656082 G68T-M122T 0.329468973519454 V35T-L47T 0.328986110730364 S43T-A259T 0.320651078524042 A38T-G256T 0.306906094527843 V35T-I120T 0.306061544654744 C30T-V258T 0.289865422382218 R70T-I121T 0.289530033483526 L66T-M122T 0.288251927327947 A45T-G53T 0.287136506022125 G53T-L252T 0.28662161340097 N39T-F263T 0.284797195594465 S43T-F263T 0.284210995688363 I48T-L119T 0.279163166788946 V77T-Y112T 0.278307021892373 A38T-I260T 0.267757602773232 G63T-L126T 0.266693894662158 L56T-S61T 0.254399405704388 L37T-S43T 0.240718382951863 L27T-T51T 0.239818227427644 T51T-L127T 0.238432802087163 I48T-G53T 0.232328855434533 L47T-I260T 0.231355970484421 V34T-I46T 0.231250122624195 Y31T-F117T 0.223251922135093 A54T-S124T 0.218571445169004 I69T-V77T 0.212875759928367 A49T-F253T 0.209444747712991 F29T-V35T 0.204758285845038 G111T-R116T 0.20373976753244 L72T-M123T 0.202110373149916 T79T-I113T 0.195163569421893 V34T-L261T 0.194461788595884 W42T-G256T 0.193847359420025 V34T-R264T 0.193263334887551 C30T-I120T 0.1914848903274 M26T-L252T 0.190482359774482 N39T-F257T 0.187067878443128 V77T-G111T 0.186715201214849 A45T-F263T 0.186463368938124 N39T-G111T 0.185885231282165 V34T-R116T 0.185701882568451 L47T-G256T 0.184828359979514 Y44T-G53T 0.181963837216967 L37T-I260T 0.179556095604794 G68T-L75T 0.178968574791189 Y44T-R116T 0.176468205336135 L37T-L261T 0.176176035088053 S57T-L62T 0.170870235289269 A49T-V115T 0.166253773544154 Y44T-A49T 0.165246167144113 I41T-V115T 0.163937276142358 C30T-L261T 0.163353189552112 C30T-L252T 0.161140163756305 T51T-I260T 0.159887403688946 S57T-M122T 0.159613563828413 V35T-I121T 0.159516799311291 I48T-I121T 0.159128091854385 F50T-A259T 0.158673246500236 V35T-I46T 0.158366528114941 A38T-V115T 0.15814377879595 S43T-F253T 0.157757081583819 L27T-G256T 0.157693001690872 K67T-L118T 0.155868486292888 R116T-S124T 0.153609962822881 I32T-T51T 0.153116675640652 Y44T-F117T 0.152867026217107 L118T-M123T 0.151047860097702 I41T-I262T 0.150140494819969 I41T-A259T 0.149836194139806 F29T-F257T 0.146220295875336 V35T-R264T 0.145547944622068 G68T-F117T 0.144294107820077 V52T-L119T 0.144259062763293 I46T-I262T 0.141691619358994 Y31T-I46T 0.141513037218611 T51T-S124T 0.139679790551709 W42T-I260T 0.139278535029369 N39T-I46T 0.137490227334303 S43T-V115T 0.137303433613496 I69T-L127T 0.136615655127555 Y31T-Y44T 0.135307914577918 I41T-I260T 0.134589672629302 V34T-V115T 0.13420128963569 I48T-V115T 0.134139729637824 G68T-L127T 0.132764869217001 I33T-I120T 0.132451986842931 L56T-L127T 0.131944203994605 V35T-V258T 0.131085007729377 Y31T-V258T 0.130153013339165 F64T-M122T 0.129908299044764 V52T-S61T 0.129741607839672 I55T-M122T 0.129584564817673 L37T-Y112T 0.129483028596235 L27T-I55T 0.128830296781985 A45T-I262T 0.128545640710128 L27T-V35T 0.128210353722114 S28T-T125T 0.127945467612435 A45T-R116T 0.126006943941914 G53T-F253T 0.124595954309717 K67T-S124T 0.124444440791676 A38T-Y112T 0.124244510060135 R70T-V77T 0.123081871905862 Y31T-A54T 0.122890389568798 A38T-G111T 0.12115091285272 L27T-L119T 0.120704926153124 A54T-W250T 0.120698774425342 G256T-F263T 0.119772418032587 G63T-P71T 0.118593183473999 I55T-S124T 0.117469719878934 R70T-T125T 0.117291917499755 V35T-M122T 0.117029324996913 V52T-L118T 0.116964704220373 Y31T-A38T 0.11661014688323 W42T-L261T 0.115890346529698 L47T-V258T 0.115512103734772 L73T-L119T 0.114148260096467 Y44T-M123T 0.11398750720365 G53T-G256T 0.113984059378526 V52T-G256T 0.112908863935431 I41T-L261T 0.112313907288038 I48T-I120T 0.111966096544029 S28T-T51T 0.11169963427624 L47T-I113T 0.110822240846339 V34T-T51T 0.110635658744837 I41T-I46T 0.11012087768714 S57T-L126T 0.109571149173612 I60T-K67T 0.108096020964951 A45T-R264T 0.106505804692662 F65T-I121T 0.104463766020453 G53T-M122T 0.103607213708789 I33T-L254T 0.102976908234694 I33T-M123T 0.102675547148337 S28T-L119T 0.102120342825359 L37T-V258T 0.101651790206553 N39T-I260T 0.101091663897257 T51T-R116T 0.100441523930898 L27T-M122T 0.100143775615779 L251T-I260T 0.100141605346137 I46T-A259T 0.0982198681637034 A49T-L255T 0.0979628637350157 L47T-L255T 0.0962416932160917 L62T-L119T 0.0961606359304255 F78T-G111T 0.0960582451993846 W74T-F117T 0.0950558036079987 L119T-L127T 0.0942119977752547 A49T-S57T 0.0939147841099643 I60T-L119T 0.0938017842234385 L62T-I120T 0.0938017842234385 T51T-W250T 0.0936542602373098 I32T-L118T 0.0933785239484184 Y44T-Y112T 0.0933281989051389 I32T-L254T 0.0922647617787689 L118T-L126T 0.0918139751576252 A45T-V115T 0.0911003360812359 L62T-L127T 0.090582939713963 A54T-L255T 0.0905574068095039 T51T-F65T 0.0905122203118389 A54T-G256T 0.0902366820531664 L72T-I121T 0.0896656856486224 S58T-F64T 0.0895203751129232 T51T-F117T 0.0887157207453153 I32T-L261T 0.0878943822851589 T51T-V115T 0.0878784998229269 L27T-L47T 0.0874999757856729 L72T-V115T 0.0871323712509391 F36T-F117T 0.0870824355546889 I46T-L251T 0.0864306193040763 I55T-L252T 0.0863352592473057 T79T-R116T 0.0861238561249302 C30T-A38T 0.0857956308014348 L251T-L261T 0.0853034142323104 C30T-L251T 0.0850054668192932 L27T-L251T 0.084796737851156 L75T-V80T 0.0846749076069209 G68T-L119T 0.0845414313142916 S28T-T249T 0.0843345052169609 S28T-W250T 0.0842673258561086 I48T-R116T 0.0829416459100063 I55T-T249T 0.0829192223938628 S57T-T249T 0.082875609738708 A45T-Y112T 0.0827170645449564 G63T-M122T 0.0821185452276065 F36T-L118T 0.0817709830272375 I55T-I69T 0.0815664922090056 A49T-V258T 0.0815644368081383 L47T-M122T 0.0811583776717124 T79T-V115T 0.0801443458984776 I33T-A259T 0.0789490317628205 G256T-R264T 0.0789154718221241 I46T-I120T 0.0787188975461033 L75T-V115T 0.0774322874201878 V80T-I120T 0.0771895046866968 V34T-I113T 0.0768933000872558 V77T-R116T 0.0767314449753087 A54T-V248T 0.0767303592092894 Y31T-L118T 0.0764971766978597 K67T-W74T 0.0761522385869675 S61T-M123T 0.0758813033450685 A54T-F257T 0.0757619015617808 I76T-I120T 0.0754670022117283 V52T-L127T 0.0753988097007577 A45T-F114T 0.075270530938166 I33T-N39T 0.0751406919750668 L66T-L75T 0.0746778832646591 I113T-M122T 0.074677753809576 L37T-I48T 0.074661013347459 W74T-L118T 0.0746054288404103 F64T-L72T 0.074528693486557 L27T-V248T 0.0744441217080675 S43T-R116T 0.0741367445902874 A54T-S61T 0.0737605124845591 I32T-V115T 0.0732575154205715 F64T-L118T 0.0730461367943382 L73T-F117T 0.0728757898964596 I48T-M122T 0.0726785170654445 L255T-F263T 0.0718247509199552 G53T-T249T 0.071401822929527 M26T-M123T 0.0707494691818599 Y31T-T249T 0.0704886600378938 A54T-M122T 0.070400629593526 A38T-L119T 0.0696103242210349 L27T-L37T 0.069545688413009 A49T-T249T 0.0690925799117181 N39T-A259T 0.0689099233304216 Y31T-N39T 0.0686272235247012 V35T-S124T 0.0679526324817048 I32T-A38T 0.0678941108211641 G63T-I121T 0.0663002030670379 L37T-A45T 0.0662797922161023 V34T-W250T 0.0661489217844506 L75T-L118T 0.0660629351083672 C30T-L255T 0.0653819120551073 V35T-L118T 0.0641574135462912 V34T-I262T 0.0640561632313874 A49T-L118T 0.0639387467951322 V80T-I121T 0.0637818721742036 T79T-L118T 0.0637708951472105 G53T-V248T 0.0625301193213046 P71T-V77T 0.062474253771174 L37T-L255T 0.06223942321793 I48T-L252T 0.0621479754720298 G53T-M123T 0.061745480768792 F36T-F50T 0.060677083087965 L37T-L119T 0.0604823867587783 K67T-L126T 0.0604519176108287 G53T-I60T 0.0599943675068708 W42T-V115T 0.0584613202986413 L47T-L252T 0.0583506217972234 L37T-I113T 0.0581275581714871 L47T-L261T 0.0579869216211248 S43T-Y112T 0.0571702430601446 A38T-F114T 0.0567168693793363 F50T-I120T 0.0562918596469856 V35T-I262T 0.0559859111149787 V52T-S124T 0.0557607289961948 I41T-R116T 0.0557439454122016 L47T-L251T 0.0552638982102005 I48T-F114T 0.0547209626890502 A49T-L119T 0.0544616117225321 G53T-S61T 0.0539902649689017 L66T-W74T 0.0536294732320099 L37T-I120T 0.0536281069052604 V34T-A259T 0.0534848221972544 L37T-A259T 0.0532437533615859 V52T-R116T 0.0530189341653274 V35T-Y112T 0.0526338030552453 I113T-M123T 0.0523815019305331 V35T-G111T 0.0522321880129211 L72T-I113T 0.0517039645686679 G68T-V115T 0.0514467864843611 M26T-L127T 0.0513155103143141 L72T-S124T 0.0507302543731114 A49T-M123T 0.049914761353012 S58T-S124T 0.0496994043165118 I48T-I113T 0.0495798225806556 F36T-I48T 0.049119748787068 V34T-L119T 0.0490720019970219 F78T-F117T 0.0489969352198392 A54T-F65T 0.0487060896471894 F117T-M122T 0.0486288662817538 L251T-F257T 0.0482106388213719 I69T-R116T 0.0481557909887598 N39T-G256T 0.0478635186982075 I55T-L66T 0.0475678471123397 G63T-S124T 0.0470901323155042 T51T-M122T 0.0464973544093464 R70T-M122T 0.0464533046673692 I32T-F253T 0.0463388517283887 L37T-F50T 0.0461280744077461 Y44T-V52T 0.0459266135500329 G63T-T125T 0.0457016678973714 L66T-I76T 0.0456220031624555 I48T-A259T 0.0455084353288546 I48T-I260T 0.0449529985817296 S57T-V248T 0.0445202390577575 P71T-M122T 0.0436547429413714 V52T-I121T 0.0435536905530087 S61T-G68T 0.0433984022815639 I55T-S61T 0.0431652361819923 F36T-I41T 0.042883050344904 L47T-M123T 0.0427522540467384 A54T-L62T 0.0426661883660118 I33T-Y44T 0.0426085029870036 M26T-F257T 0.0425481781586496 L66T-S124T 0.0422935973644001 S43T-L255T 0.0421697896852982 L27T-L252T 0.0419765500496307 T79T-G111T 0.0411572123192727 I46T-M123T 0.0410948492608263 L118T-L127T 0.0410690071313269 S57T-L127T 0.0402556228946409 G111T-L119T 0.0385754172033552 C30T-L119T 0.0367729435682183 G68T-L73T 0.036318971970597 V35T-A45T 0.0361679263267281 A38T-I262T 0.0358843019554342 M26T-T249T 0.0354767334330566 Y44T-I260T 0.0352993250907669 V52T-F253T 0.0349282759000533 G53T-L62T 0.0349185653774811 F36T-T51T 0.0340688804134048 I69T-V80T 0.0340098531212618 V34T-N39T 0.0333815729352223 A45T-L261T 0.0333787156283213 T51T-G256T 0.0333573177520397 F64T-P71T 0.0330393376359683 Y44T-F257T 0.0328982231876456 I55T-G63T 0.0328374575507353 I46T-T249T 0.0323224528429105 F50T-V248T 0.0313673394002996 F50T-F253T 0.0312255912147048 L27T-A54T 0.0309320142213894 Y44T-A259T 0.0301755173141727 L66T-L127T 0.0299910230146576 L56T-G63T 0.0296651380442112 P71T-S124T 0.0292636186772928 R70T-T79T 0.028912500065835 F36T-I46T 0.0288329143615256 I33T-L119T 0.0286570971120153 S61T-M122T 0.0278967240415346 F65T-L127T 0.0278809137758369 T249T-A259T 0.0274004471486791 I41T-F114T 0.0270156084684636 G53T-L126T 0.0257045184294413 F114T-M123T 0.0255868713399439 W42T-L255T 0.0254850958182444 I60T-G68T 0.0254373144662585 F29T-F36T 0.0253505549104744 A38T-V258T 0.0252552438190117 S43T-L119T 0.0251711655703559 M26T-S124T 0.0245850907584495 N39T-I48T 0.0245170479658201 F50T-L126T 0.0244118930748698 V81T-V115T 0.0243876999885089 V34T-L255T 0.0240768120252497 I46T-R264T 0.0240235280118206 L27T-G53T 0.0235367640169986 L37T-V115T 0.0231390677635364 L47T-F117T 0.0228905963725512 V35T-A49T 0.0225385813869314 V34T-L118T 0.0222490919610556 A54T-L119T 0.0207481710531727 L27T-V52T 0.0202713176178482 I69T-F78T 0.019560669920299 S43T-I48T 0.0195250091161903 P71T-F114T 0.0193677949959292 T51T-S61T 0.0183378384334931 I32T-I121T 0.0182336266544123 A54T-L251T 0.0182087866203258 F50T-M122T 0.0180852629829911 A38T-F253T 0.0175019728659951 R116T-I121T 0.0173313494858749 F78T-I113T 0.0173090590645049 F257T-R264T 0.0172932898786016 V34T-L251T 0.0168615144443791 F65T-I120T 0.0168163639028214 L66T-V77T 0.0163711993215441 Y31T-T125T 0.016285509625956 L127T-W250T 0.0158513405748897 V34T-M123T 0.0156736162984465 S58T-G63T 0.0155109888493321 Y31T-I120T 0.0154322702059666 V258T-F263T 0.0151940007330102 F36T-M123T 0.0147727770076225 F64T-I121T 0.0145829639280728 F29T-I120T 0.0144340574912993 S58T-F65T 0.0143519713937778 S28T-F253T 0.0141521415426218 A54T-I120T 0.0128349897230935 F29T-V34T 0.0123752584655248 L56T-F65T 0.0121486915012472 A49T-A259T 0.0118074676414963 A49T-F257T 0.0116758481355106 V258T-R264T 0.0116067249617489 A49T-M122T 0.0112660595887566 A38T-I48T 0.0111160010443996 S61T-K67T 0.0109880873452275 Y44T-I120T 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