[Wed Aug 24 18:55:03 2016] >Input file: TM4JKVATD.pdb >TM segments: A229-254_A263-287_A312-340_A358-378_A398-424_A451-475_A515-537_B229-254_B263-287_B312-340_B358-378_B398-424_B451-474_B515-537 MAIDEN score = -209.8534 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C273A-A324A 10 C273B-A324B 10 C469B-E518B 6.0364682802267 H470B-N521B 1.35711921203407 I266A-L335A 1.22374214932935 W281A-I317A 1.21555848353956 H470A-N521A 1.20328819041337 C469B-V517B 1.12924240510129 I266B-L335B 1.11246323541176 W281B-I317B 1.05371350646406 Y323B-F369B 1.0358791823127 V276A-I320A 1.02071656090465 V276B-I320B 1.00234250553018 V270A-W331A 0.924339415009717 V270A-F332A 0.913430113282247 V270B-F332B 0.910557233390338 W339B-L452B 0.898851636662557 V270B-W331B 0.890939935952535 T466B-N521B 0.870088021931945 Y322B-T372B 0.857324545462937 Y323A-F369A 0.833389458393968 V329B-I408B 0.831261160567616 C469A-V517A 0.830009092591979 T248A-F268A 0.824788181281405 T241A-F274A 0.822631597091452 H340A-G422A 0.803093570999408 T248B-F268B 0.802782346349765 T241B-F274B 0.801049403280762 T241B-F275B 0.796236299166619 L363B-L363A 0.784959975532211 T466A-N521A 0.769530153758495 L367B-L367A 0.765630312448224 I234A-W281A 0.759592739943821 I234B-W281B 0.753369507098612 W339A-F418A 0.749383361218613 I266A-W331A 0.747852593027921 T245B-F274B 0.747212956555495 F462A-M525A 0.730250567269745 F252A-F268A 0.730056960566653 W339B-F418B 0.72260401110893 M326A-P368A 0.720983219223388 F462B-M525B 0.720630446143819 L405B-F467B 0.704987523944673 F252B-F268B 0.70486781272994 W281B-V321B 0.702568968594858 L405A-F467A 0.700390399557627 L249B-F268B 0.699661639857048 V329A-I408A 0.699077466511895 V319B-T372B 0.697323004519188 T241A-F275A 0.696372655761584 L249A-F268A 0.686992243557306 F318B-Y323B 0.684676871151737 Y337A-L419A 0.679468407641235 F455B-W535B 0.678492105707329 R400B-H470B 0.676175645346312 F237B-F526B 0.674488895237049 R400A-H470A 0.672985550058899 Y337B-L419B 0.669723718015014 A327A-W365A 0.668703502034159 F274A-A324A 0.667143127569393 A327B-W365B 0.66674309848634 T248A-T534A 0.666158844426349 W281A-V321A 0.663710189664799 F455B-M532B 0.659060342537909 C244B-I530B 0.656201190839076 L412A-L464A 0.654504365750151 T251A-V536A 0.643366221605321 V270A-A327A 0.643259710133234 C244A-I530A 0.639060268596803 F274B-A324B 0.638932105135003 T245A-F274A 0.636497536263938 L246A-F275A 0.632294072904619 F455A-W535A 0.630982317592595 V319B-A324B 0.62516096705331 G242A-F274A 0.624996695533804 G415A-F460A 0.623272478901523 L412B-L464B 0.623006198432008 V270B-A327B 0.622759617920175 G242B-F274B 0.621698640708119 A401B-V378A 0.61927897199328 V378B-A401A 0.619162768090344 F332A-G415A 0.618962556664751 G415B-F460B 0.618783814259043 G416B-F460B 0.616862715695036 G416A-F460A 0.615896750533988 F332B-G456B 0.615433807546655 F462A-G527A 0.613976101933541 F332A-G456A 0.613957676885236 F332B-G415B 0.61304974593293 F462B-G527B 0.612579816364557 H340B-R421B 0.612177027331666 W339A-L452A 0.610901997291093 F455B-A531B 0.606958866515073 I266B-W331B 0.602530395849077 L405B-H470B 0.598203485576748 F455A-M532A 0.597262454892963 G280A-F318A 0.592946144393041 L246B-F275B 0.592091544277263 F455A-A531A 0.590992155140237 M326B-P368B 0.590791335275226 P263A-L335A 0.583836375271725 C469B-N521B 0.583658703630709 L405A-H470A 0.583039571098157 P263B-L335B 0.58267102807168 G238B-F526B 0.578985900082582 F237A-F526A 0.576661361150346 A239A-L282A 0.570446800753652 A401A-F474A 0.570371668706742 R398B-I375A 0.570195162842397 I266B-V334B 0.56815278145506 T251B-V536B 0.567538416086215 Y399B-A377A 0.56591069337218 V319A-A324A 0.565638795473545 Y269B-W331B 0.565450353587584 Y399B-V404B 0.564712092660162 Y269A-W331A 0.564613167072444 F275B-G529B 0.561183431724403 F462A-T528A 0.55936981163944 A377B-Y399A 0.55914064104196 A459B-M532B 0.557860540215455 A374B-F403A 0.557698431290466 Y399A-V404A 0.553524312775548 L405A-F471A 0.548817952982482 H361B-H361A 0.548427090148879 F275A-G529A 0.544925160971157 G280B-F318B 0.540081757166704 Y472A-V517A 0.539826068928705 V319A-T372A 0.535234512077429 D473B-V517B 0.532538348671633 T245A-I530A 0.52990532938469 F274A-L325A 0.52697964614273 L419A-F457A 0.526874561252663 F274A-G328A 0.524545739296001 A401B-F474B 0.52397393736341 I266A-V334A 0.521520688584745 F332B-A459B 0.520916969079431 L405B-F474B 0.519524330463341 A235A-F285A 0.519291663390409 A374B-Y399A 0.517371364016221 T248B-T534B 0.517148780779324 V404B-H470B 0.515507039565684 A459A-M532A 0.513111642347802 V463A-A524A 0.511923505490478 A235B-F285B 0.510682021359147 F274A-F526A 0.509518890054272 I408A-V463A 0.509076237769984 N271A-G529A 0.50883452283333 A406B-V373A 0.50725311454991 V333A-V411A 0.505994803450442 F318A-Y323A 0.502916563404119 F274B-L325B 0.50273479771481 Y472B-V517B 0.500997085530319 F237A-L522A 0.500718446410623 Y337A-G415A 0.500696601603427 V463B-A524B 0.499647109269596 L419B-F457B 0.499092346067797 L405A-F474A 0.498680515562992 Y233A-K519A 0.497759792373872 V270A-G529A 0.496287758335056 F332A-A459A 0.495966444824845 A401B-F471B 0.493844827344354 F274B-F526B 0.493772897321096 C469A-I520A 0.49029449750175 A401A-F471A 0.487617625491824 P263A-L452A 0.483442522256256 C244A-C273A 0.483408391063509 M230B-K519B 0.479755550224737 I234B-L282B 0.479016617567371 M230A-K519A 0.47896554301605 I234A-L282A 0.478501380224094 V270B-G529B 0.477867706921243 F237A-F523A 0.476566939599592 F274B-G328B 0.476238674843358 V373B-A406A 0.475958264138422 S278B-V321B 0.47402880702644 S278A-V321A 0.473439010255525 A239B-L282B 0.472232870217622 L405B-F471B 0.472231650912983 L249B-A272B 0.471538827426739 M326A-I408A 0.471248663032179 H340B-V423B 0.471214252089262 I375B-R398A 0.46686091469231 L363B-F360A 0.464768234007217 Y337B-G415B 0.463908022448883 C244B-C273B 0.458200881263314 R398B-F474B 0.457789189584657 Y233B-K519B 0.456812781124299 G402B-I375A 0.455288721117778 F403B-A374A 0.452344083209088 R400B-F474B 0.451863099772478 L249A-A272A 0.45156845526753 C469B-I520B 0.449263016480193 C244B-M532B 0.448407468732437 R398A-F474A 0.447720116495706 T336B-G453B 0.446198324256223 T241A-G527A 0.44593889794985 R400A-F474A 0.444964008806602 W339B-L419B 0.443864854920545 F237B-F523B 0.443803139179004 L267A-S533A 0.443023703889149 T241B-G527B 0.442968520534001 C244A-M532A 0.442383054332609 L335B-L452B 0.441910752248068 L371B-F403B 0.441168168553946 W339B-G456B 0.440143510713638 I375A-F403A 0.438982124913562 L267B-S533B 0.437127599426474 V404A-H470A 0.436466710285117 G238A-F526A 0.435226349989787 Y399B-A374A 0.431196367410099 N271B-G529B 0.430641750174352 G242B-I279B 0.426165855031572 L412B-F460B 0.422899505576597 H340A-V423A 0.419935571978615 H231B-M286B 0.419086486409447 L371A-F403A 0.417483191804546 C244A-S533A 0.415906141234391 V370B-L410A 0.413131363411122 A406B-V370A 0.409844050850191 I375B-F403B 0.408763850394363 W339A-L419A 0.404056668645219 C244A-T534A 0.403473110004388 F462B-T528B 0.403204742863528 C469A-N521A 0.402561544870963 V315A-L376A 0.402283722901802 M326B-I408B 0.401854609260758 V411A-G416A 0.400634010891871 L410B-V370A 0.400527818629297 T241B-I530B 0.399951059948926 V370B-A406A 0.399860364202402 H231A-F285A 0.396159693331722 H340A-R421A 0.395875737726184 I465A-A524A 0.394934625892144 L412A-F460A 0.39420413889383 G238A-I279A 0.390551801828162 G238A-W281A 0.389614145012145 G242A-I279A 0.3894116956735 L412A-Y417A 0.38921104392815 T245B-I530B 0.388419227147738 M525B-I530B 0.388169159355296 I234A-L522A 0.385853922098828 A235A-W281A 0.384814882190366 I465B-I520B 0.384803800852475 W365B-V370B 0.384133149148496 I465A-I520A 0.383709588443479 H231A-M286A 0.382973320442532 I375B-G402A 0.377768312459586 I465B-A524B 0.377477440688642 G402A-P407A 0.376327386198497 V333B-V411B 0.375013428424527 V330B-L367B 0.373290591319296 W331A-T364A 0.372212269956399 T466A-L522A 0.367218886282033 P263B-W331B 0.364472207982952 V315B-L376B 0.364323822995483 G277B-V321B 0.363377968269087 A235B-L282B 0.363333808875589 A235B-W281B 0.362275806409887 L458B-T528B 0.362185190167326 V240A-I530A 0.3602308446115 C273B-F318B 0.360062816913157 A235A-L282A 0.359040738272418 T336A-G416A 0.358938988783783 V276A-W281A 0.357450647530746 H340B-L419B 0.357402395978252 V373B-Y399B 0.35702866302523 V334B-Y359B 0.35656718197169 Y233A-G527A 0.354633953343586 C273B-G280B 0.352865400765919 C273A-F318A 0.352141461364055 G277A-V321A 0.351498164935433 T245A-N271A 0.350773832886029 G402B-Y399A 0.35005554354543 P263A-W331A 0.349669545323622 H340A-L419A 0.349197092405344 G238B-W281B 0.348714893528236 T336B-L452B 0.348591709591722 V333B-G415B 0.34828931176484 T245B-A272B 0.347130004442526 A239A-S278A 0.34550164130838 I408B-V463B 0.345321240821545 M525A-I530A 0.34513056464838 T336A-G422A 0.345030133743049 M230B-L515B 0.345006115133592 M326A-L367A 0.343809811186381 M230A-L515A 0.343323580064322 I408A-F467A 0.342895710579476 I279A-I320A 0.342760666519411 T466B-L522B 0.342400101643118 T245A-A272A 0.341701411021496 D473A-V517A 0.340683688102956 T466A-I520A 0.340298181784676 M326B-L367B 0.340289813739984 L458B-A531B 0.339806458862428 A338B-L452B 0.338728425918913 T241A-I530A 0.338359691205611 I408B-F467B 0.336959963292181 C469B-F526B 0.334354215620338 C469A-F526A 0.33220081298473 F474B-E518B 0.332084194394585 W331B-T364B 0.331954233339481 I279B-I320B 0.331712796996574 V330A-L367A 0.330204743323827 H231B-Q284B 0.33014128597855 C244B-S533B 0.32914652346944 V404B-D473B 0.325348980967515 T466B-I520B 0.325003642742019 W339B-G422B 0.321080392238557 A406B-S468B 0.319946183354716 A239B-S278B 0.319786393165364 V373A-Y399A 0.317475120907208 A327A-L367A 0.316458883260549 V333A-G415A 0.315913050846595 V404A-D473A 0.315474555153316 G277A-G527A 0.315010023403925 V330B-W365B 0.314295617866621 G456A-G529A 0.314035513094961 G409B-G461B 0.313995031644101 L267A-W535A 0.313927382938662 L371B-L371A 0.313912071222869 G328B-G415B 0.313674011123356 Y233B-Q284B 0.313208309896726 W339B-V423B 0.312125427838152 G328B-G456B 0.311486372123154 L458A-T528A 0.311298656285917 I375A-Y399A 0.311162937732017 A235B-G280B 0.311137115047569 H231A-Q284A 0.311098862807946 F237B-L522B 0.310395370921476 G328A-G456A 0.31023042659932 Y359B-R421A 0.309133851286725 V414B-Y359A 0.308504660642563 P263B-L452B 0.308139720512177 C273A-G280A 0.307491420732169 Y233A-Q284A 0.306036037209692 A235A-G280A 0.305456888242619 V411B-G416B 0.304592764970478 A239B-G529B 0.303292452920342 A264A-G453A 0.303259021746207 G328B-G527B 0.303094814183826 F474A-E518A 0.298683228555568 G409A-G461A 0.298250509831686 T364A-P407A 0.296323771146978 Y337B-F418B 0.29629444062153 G277B-G527B 0.295064995120184 Y233A-V517A 0.295005701292628 A406A-S468A 0.295000708459898 C273A-L325A 0.294688473024758 Y399B-G402A 0.294654453682273 F332B-V411B 0.293984109375566 W331B-W365B 0.293633976463849 I234A-K519A 0.293284382047225 L246A-A272A 0.29310396151841 G328A-G527A 0.293055985344713 G242B-A283B 0.292857353639144 V240B-I530B 0.292243371844221 L335A-L452A 0.292027334921691 V334A-F360A 0.29192278375837 L267B-W535B 0.291196167261536 Y233B-V517B 0.291092338718864 L412B-V463B 0.289698555813796 S278B-G527B 0.28915665477183 T241A-F526A 0.289021097264046 A459A-S533A 0.288322379387884 P263B-W535B 0.288121496233428 G328A-G415A 0.287158247603933 G328B-H361B 0.286420325836032 I375B-Y399B 0.286017110632575 L246B-A272B 0.285385706186184 G238B-I279B 0.284911512776524 L267B-M532B 0.283805705320873 Y322B-G402B 0.283784721437343 F332A-V411A 0.283431665511197 S278A-G527A 0.283246694641522 V329B-V463B 0.28180462784376 L267A-M532A 0.281701076756987 H340B-F418B 0.281136378499689 A327B-L367B 0.281118473496523 G409A-F467A 0.281078203179246 G409B-F467B 0.28069806509477 L458A-A531A 0.280623471222471 T241B-T528B 0.280525369442303 T241B-F526B 0.280455224744822 A239B-G280B 0.280397328400487 L335B-G422B 0.279340457426572 A264A-L452A 0.278978868249674 W339A-G453A 0.278843319855508 G402B-L410B 0.278764561893819 T248A-C273A 0.278091512408047 A401B-A374A 0.277917282469047 L412B-G456B 0.277654037239951 L267B-L335B 0.276718141765298 C273B-L325B 0.276551948220336 G453B-G461B 0.276434745172814 L412A-G456A 0.276379704986844 Y359B-G415A 0.275288026292573 P263B-A338B 0.27429543228714 N271B-G527B 0.274276285277613 G456B-G529B 0.273866379642582 C273B-M326B 0.272536989631738 V378B-Y399A 0.272121385568154 L362B-G415A 0.271962660157409 G238B-L282B 0.271828473450014 L412B-Y417B 0.271705475763346 C273B-V334B 0.271545258158092 L267B-W331B 0.271242670605183 V463B-T528B 0.271224494158642 I234B-A239B 0.270792853435267 G238A-L282A 0.267871377314786 T248B-C273B 0.267376662788604 P263A-W535A 0.267082192666603 A239B-G277B 0.266188638167383 C273A-M326A 0.265616332433979 H361B-Y337A 0.265434279443583 A338B-R451B 0.264013913116647 A338A-R421A 0.263770354700433 A401B-H470B 0.262140697164956 N271A-M326A 0.262076183866252 Y322A-G402A 0.261620988106047 N271B-M326B 0.260440376241712 N271A-G527A 0.260260353170599 V319B-V373B 0.260226958674587 F332B-F460B 0.260128134665837 F332A-G529A 0.258078733915116 G328B-F526B 0.257801150581615 H361B-L363A 0.257158869702239 G328A-F526A 0.2570214839354 W339A-V423A 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