[Wed Aug 24 19:09:54 2016] >Input file: TM4K5YATD.pdb >TM segments: A121-142_A153-167_A198-212_A225-239_A275-288_A316-330_A339-363 MAIDEN score = -51.8313 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- S130A-F357A 7.92762253094133 L134A-I163A 1.22991346884449 L131A-I163A 1.22596301466024 L134A-I159A 1.22255996564489 I154A-W205A 1.11455516994703 L158A-W205A 1.02915995530338 F203A-V279A 0.962012955799057 F141A-H155A 0.905254559496555 P239A-P275A 0.855855666616331 A161A-W205A 0.855137253110947 F204A-V279A 0.835531788165305 R165A-H199A 0.815186259649583 C211A-F286A 0.799076443725554 L135A-I163A 0.799014244720657 W205A-I234A 0.75372209651249 L134A-S360A 0.721328872285903 F207A-V279A 0.712444858965397 W205A-G235A 0.701668813238229 C233A-V238A 0.679708477852865 L131A-A167A 0.67618972978359 I129A-F357A 0.672117889570133 I122A-S353A 0.666926604864325 I129A-F354A 0.651481090312228 G208A-I234A 0.635780237787467 A137A-V361A 0.629609936014771 A133A-V361A 0.625042990464291 L323A-E352A 0.624123861353645 A161A-F198A 0.615255588026372 C211A-N283A 0.594834120267159 F204A-W236A 0.592237245326106 F141A-A156A 0.590132372407284 L322A-Q355A 0.586251902237045 F203A-M276A 0.574808668548068 I232A-G237A 0.568215711474929 F138A-A160A 0.565376193726506 I153A-A228A 0.557507156685283 N157A-I232A 0.536139268773505 F203A-L280A 0.525709620631121 L131A-F357A 0.525152011547641 S130A-S360A 0.521566905052315 L134A-F162A 0.518617373124445 L319A-F362A 0.50506370366482 L125A-S353A 0.495843475575839 G210A-N283A 0.49342969038776 L322A-F358A 0.492170247363116 F162A-F198A 0.486280829969634 I154A-G208A 0.482588291353707 V200A-M276A 0.481297302058165 F204A-G235A 0.472087749623792 L319A-F358A 0.4686754188325 P321A-E352A 0.468487134931681 T201A-W236A 0.465701552213183 G210A-F286A 0.462432324535545 S130A-S353A 0.45742918791014 I129A-S353A 0.457277674916646 F138A-A156A 0.451855610381979 N157A-W205A 0.451828923682426 L158A-M206A 0.446276554249025 F138A-I159A 0.445882955589802 L134A-F357A 0.445403799264267 L322A-L351A 0.442854276291554 Y124A-S353A 0.441465528220267 I154A-E209A 0.438867246144635 I234A-P239A 0.436020388178407 I159A-V359A 0.433747160143932 N123A-F350A 0.432182276304252 L131A-R165A 0.431173071804189 F207A-L280A 0.430632185371676 F204A-I234A 0.422886169034416 L164A-F198A 0.422391929079114 I154A-F231A 0.414882564651863 H155A-C233A 0.412079046782468 H127A-H199A 0.410933196079515 L329A-N348A 0.403324784866347 F207A-I234A 0.40258024002631 R225A-C233A 0.40200628545891 L319A-V359A 0.399349103670337 I122A-Y346A 0.398867632339829 I122A-A349A 0.392917820385666 I163A-G356A 0.37601568972193 L142A-A156A 0.367009249859891 A160A-A363A 0.363079069363647 M206A-C211A 0.355677325590119 F204A-P239A 0.354170653704877 C211A-C233A 0.354125950469124 I122A-F350A 0.351389730154074 I154A-M206A 0.349112837192419 A137A-I159A 0.348170405083119 W205A-F231A 0.347300274407024 H127A-Q355A 0.344234030299833 P321A-Q355A 0.341240206931259 G126A-F350A 0.340310886457764 L323A-L351A 0.334721789611702 N157A-R165A 0.334312267598241 F344A-E352A 0.331663315014205 L158A-N202A 0.322428841429645 A133A-A160A 0.32157474907819 F162A-E352A 0.314703228303336 F198A-C233A 0.307978031690157 N157A-M230A 0.307290492380583 E209A-F284A 0.307030190631465 P275A-N283A 0.303157771777876 A133A-F357A 0.302721217839729 H155A-C211A 0.301580416897961 L134A-A160A 0.300420513113477 L134A-V359A 0.297905348454678 L322A-E352A 0.297124358344164 A156A-A228A 0.292966109608551 F207A-I282A 0.291494652557319 A330A-A349A 0.288908040015482 A161A-S360A 0.288149518228711 S130A-F354A 0.285616985503887 L135A-A160A 0.282118755682459 V200A-V238A 0.281766304493981 F162A-G356A 0.28162133528006 A156A-G208A 0.280027151285816 G210A-L281A 0.274388202558796 A161A-G208A 0.270760486099674 N123A-G356A 0.266658687763352 N202A-P275A 0.259075525820083 L131A-S360A 0.255835977506762 G208A-W229A 0.254482357312615 S130A-Q355A 0.252489304664581 H199A-Y327A 0.249470529790999 A137A-A363A 0.249000711477509 N166A-Q355A 0.245240903647386 L319A-G356A 0.241964574838407 R341A-Y346A 0.23615476595926 L134A-N166A 0.233950788282569 G208A-F286A 0.231898569180325 A133A-A363A 0.230562061039858 V132A-I159A 0.229576297636361 N123A-S130A 0.228913535427188 C128A-Q355A 0.223987021250426 P321A-T326A 0.223196498585004 F162A-A363A 0.222934754865495 Y124A-G356A 0.221650840106268 H199A-P275A 0.2194807154933 Y124A-A167A 0.21642849765218 G210A-L287A 0.212947788086887 H127A-F354A 0.211922214731901 G208A-I282A 0.211433516463142 T326A-L351A 0.209253514992401 F203A-V238A 0.209069084556109 G235A-V279A 0.208811335755826 F138A-V361A 0.208712607425421 G126A-F354A 0.208079082979033 N202A-G210A 0.207712899131437 F138A-V359A 0.204528206270664 L323A-V359A 0.204457108809319 H155A-N202A 0.204192419922878 G324A-N348A 0.204014018169803 C211A-I232A 0.203540099752648 N157A-G237A 0.202490522623516 Y212A-G235A 0.201535157072401 W205A-W236A 0.199068316038185 G126A-N348A 0.19855195953401 Y212A-C233A 0.196324347438202 L140A-N157A 0.196019052249779 G208A-P275A 0.19527847918666 F162A-G235A 0.193599475509298 W236A-M276A 0.193065457770897 S130A-F358A 0.190906568258772 F162A-V359A 0.189169222441514 H155A-G356A 0.188986613820447 N202A-F231A 0.187531373194728 N123A-L351A 0.187322754176302 L131A-G356A 0.18564350222155 S353A-V359A 0.185442719332841 N202A-G237A 0.185043285688794 N202A-F358A 0.18416535403616 Y327A-F350A 0.18404942352782 H199A-V238A 0.182322942730255 N166A-G356A 0.181582942411774 V200A-W236A 0.181095430795071 W205A-Y212A 0.180923413713487 M276A-G324A 0.180725222003696 G210A-F288A 0.179785155279678 C211A-W229A 0.179181047598719 L323A-S360A 0.178688731900648 T201A-E209A 0.178594056918412 I154A-G235A 0.177710616893005 Y124A-Q355A 0.176102693392324 V132A-F162A 0.175846062418818 N166A-F358A 0.175797575830841 I153A-A363A 0.175179417282362 A156A-G235A 0.174820177497987 G210A-L319A 0.173345751820499 A277A-Y327A 0.172613477899538 L135A-I159A 0.172325048947853 H127A-V359A 0.172278835171817 Y124A-Y346A 0.172056235255165 T316A-F358A 0.17192706629652 R227A-I232A 0.171573404554304 H127A-F198A 0.171239278282337 L158A-N166A 0.170711772988228 G210A-I282A 0.169191752495431 C211A-L280A 0.168372455515529 A156A-G210A 0.167471413222392 H199A-V279A 0.165483190429104 G235A-A277A 0.164388862431307 H199A-M276A 0.16403623045941 G126A-F358A 0.161847461444141 H155A-Y212A 0.159916084478699 F347A-Q355A 0.159793013670753 Y124A-F357A 0.158750300758962 F138A-F358A 0.158302949543016 I159A-I232A 0.157378905675165 L134A-G356A 0.156668425051806 T326A-F354A 0.156089897953843 Y327A-F344A 0.156039909108232 C128A-N166A 0.155269469304679 R165A-F198A 0.15522431784306 N157A-I234A 0.154963815605797 A156A-T201A 0.154953940066597 G208A-L287A 0.154526616277037 P321A-G356A 0.153532119565805 L287A-T316A 0.150623235052124 A161A-I232A 0.150458439956358 T316A-P321A 0.147893138416233 H127A-A161A 0.14753096378284 Q355A-A363A 0.145787065142767 P321A-L329A 0.14241119958876 L140A-I154A 0.141931734502156 V139A-S360A 0.141553235479904 A161A-Q355A 0.141110133293395 C233A-P239A 0.140081639098728 L131A-S353A 0.138732983164541 V136A-H155A 0.13560374100939 G208A-F231A 0.135496859603631 G208A-G235A 0.135340069931174 H127A-S360A 0.134768545388227 N157A-S360A 0.134203545797607 N202A-N283A 0.133800841783634 N202A-A277A 0.132840021900407 N123A-A167A 0.132797811519395 T326A-G356A 0.132366256272696 T201A-A277A 0.132179274152751 F207A-F231A 0.130332514836109 A156A-N202A 0.129966423486625 L135A-V359A 0.129476720682022 I325A-Y346A 0.129329294735874 I163A-A363A 0.128053756329034 I159A-A363A 0.12795819362854 F203A-Q355A 0.127364149499627 F198A-G237A 0.127057647063817 M206A-V238A 0.126487577744841 H155A-I232A 0.125520361307869 V132A-F354A 0.125169088622053 Y327A-G356A 0.125009158969402 N123A-E352A 0.124893656591853 L280A-L319A 0.124398618762513 A133A-A161A 0.123389686868888 I153A-I234A 0.123083018539748 C128A-S360A 0.122780981354727 L140A-I163A 0.122282632862784 A167A-G356A 0.122266188667588 G210A-A277A 0.121843718690098 F162A-F207A 0.121440158925877 C128A-F354A 0.121382986124364 R165A-Q355A 0.121378959300366 Y212A-I285A 0.120861898526757 N202A-E209A 0.120112883598844 L287A-L319A 0.119905516388289 A330A-S353A 0.119474948066127 F204A-F231A 0.119416668899508 F203A-G324A 0.119129177622023 G210A-I234A 0.11725191380919 G126A-F347A 0.116675992711089 I129A-A349A 0.116613142420945 A156A-F362A 0.1165764914776 F204A-M276A 0.116353381398134 G210A-A363A 0.115963724176653 N202A-S353A 0.115877530249919 M276A-L323A 0.115220642102138 L134A-N202A 0.114683783251365 L322A-S360A 0.114507282124483 E209A-C233A 0.114135410651912 A330A-F347A 0.113994027175623 L280A-M328A 0.113443063583745 H127A-N166A 0.113201476674988 I121A-E352A 0.113192873552145 S130A-L164A 0.112710749778211 H155A-G235A 0.111932668332514 I153A-I232A 0.11167205203449 G208A-T316A 0.111666215947402 F162A-F204A 0.111568128305895 I234A-I282A 0.11155451025297 G208A-V238A 0.111343419024383 L322A-L329A 0.111169701915278 L134A-H155A 0.110839139532033 G126A-V359A 0.110798991680146 L135A-L158A 0.110270790007633 N202A-E352A 0.109921074215691 A156A-V361A 0.109745088302528 M206A-G324A 0.108868112528167 W205A-G237A 0.108834697640015 M206A-P275A 0.108577580304411 G208A-A363A 0.10856922478573 T316A-V361A 0.108275273752381 A137A-I154A 0.107954284476937 G208A-G237A 0.107686447544951 V136A-S360A 0.107320400655104 F204A-V238A 0.107026312826313 L322A-S353A 0.106956086811563 L131A-A161A 0.10657986132037 A349A-G356A 0.10630925590898 I153A-R225A 0.106284901532944 P239A-L278A 0.105534071112254 L319A-Y327A 0.105399354543048 L125A-L351A 0.105129104301033 V200A-L278A 0.104924313455732 A137A-A161A 0.104749778723797 R341A-A349A 0.10437821972604 N348A-Q355A 0.104244392741752 V132A-A363A 0.104233560333828 H155A-V361A 0.10421888297251 A330A-V343A 0.103743493391583 G126A-I163A 0.103571194266819 N157A-N166A 0.103441968681344 V139A-L158A 0.103354265793747 L317A-V359A 0.102506297956179 Y327A-I345A 0.102165454952743 L281A-G324A 0.101898770184212 G208A-A277A 0.101257375841219 N157A-W236A 0.100913202088938 A277A-I285A 0.10032366196112 G208A-M276A 0.100042122793137 A228A-G237A 0.0999733651780412 V136A-L158A 0.0998526643685123 M206A-G356A 0.0995962627682605 S130A-A161A 0.0990105672480723 A156A-C233A 0.098512087886514 A167A-S360A 0.0984155479899338 A133A-L164A 0.098118038322516 A160A-V200A 0.0980308744204716 I159A-S360A 0.0973906148225168 F203A-Y327A 0.0971199318637908 G324A-G356A 0.0967769320120444 F350A-F358A 0.0965239324687608 T316A-G324A 0.0963017593002992 I154A-C233A 0.0959455443373695 H127A-F350A 0.095616918269303 A161A-A363A 0.0955339707536504 S130A-N166A 0.0950007550783845 M206A-L317A 0.0945198568161638 S130A-A363A 0.0943756718945878 H155A-A228A 0.0941752078112463 L319A-L351A 0.0941707186043401 T316A-S360A 0.0940634764360297 L134A-I153A 0.0938017842234385 L329A-V342A 0.0937132702345154 F231A-G237A 0.0929673204624057 V139A-V359A 0.0925107738237259 V238A-A277A 0.0924487547839417 W205A-L280A 0.0923903007475926 H127A-A160A 0.0923130762281579 I122A-L131A 0.091580915474303 G237A-P275A 0.0915313261854127 V139A-V361A 0.091524260690016 N123A-T326A 0.0914473575020489 H199A-G237A 0.0913285034740197 V132A-S353A 0.0910867193046463 I154A-W236A 0.0908968052529371 A156A-F203A 0.0907613490802581 V318A-Q355A 0.0903924199518171 P321A-N348A 0.0903193118422347 V139A-I153A 0.0901509202535733 N202A-C233A 0.089906513484074 I154A-V359A 0.0898153677348584 N123A-Q355A 0.0894393222154929 F138A-L158A 0.0883910472571632 L322A-A349A 0.0883478619803197 F204A-L320A 0.0882135092801049 L142A-A363A 0.0880956839257863 L164A-G235A 0.0880914289434624 H127A-L164A 0.0877453384801896 M276A-I285A 0.0876968367345179 Y327A-S353A 0.0876860419936403 Y212A-I282A 0.0875474776661029 L131A-L158A 0.0875206960830308 I163A-H199A 0.0871428598811708 L351A-F358A 0.0869102227913296 L140A-L158A 0.0867313008558939 A133A-G356A 0.0866234051425101 N157A-R225A 0.0861428744867583 L320A-V361A 0.0859296589023615 N157A-C233A 0.0856199013611558 N202A-A363A 0.0851160375621616 A133A-A167A 0.0850399585703663 L281A-L323A 0.0848389750519791 C128A-A349A 0.0846733558738346 E209A-F362A 0.0842913860064378 A133A-L351A 0.0841428632096166 R165A-S353A 0.0840413278971457 L158A-L323A 0.0839791442281852 E209A-M276A 0.0839741469062094 T326A-F358A 0.0838519546621921 M206A-P239A 0.0833631872290688 I285A-T316A 0.0831385228369861 I159A-I234A 0.0831003822001717 I163A-M206A 0.0830203317108573 H155A-T201A 0.0828143084219013 A133A-S353A 0.0828075943144606 V200A-L280A 0.0827066185651772 L320A-Y327A 0.0826372306266272 H155A-G210A 0.0825893184094459 N202A-W236A 0.0814334128329706 P239A-A277A 0.0813832644582746 A156A-F358A 0.0812464185447102 L158A-H199A 0.0810856708328416 F284A-T316A 0.0810450825310109 N202A-V279A 0.0802563343941896 L323A-Q355A 0.0798012669350707 H199A-G235A 0.0795404788601672 V139A-I163A 0.0791881298582391 F207A-G237A 0.0790564245792331 V136A-A363A 0.0789376621254165 I234A-A277A 0.0783590560157006 G126A-R165A 0.078276914212797 C211A-G235A 0.0782231534179001 L164A-F357A 0.0782173690870733 H155A-I163A 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