[Wed Aug 24 17:17:59 2016] >Input file: TM4KJSATD.pdb >TM segments: A27-49_A59-81_A84-110_A126-148_A154-175_A205-228_A237-261_A271-297 MAIDEN score = -59.1330 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- S163A-P288A 10 S107A-I260A 9.15583072497401 I38A-V250A 1.88857457501933 I75A-E222A 1.31477385436597 M166A-M284A 1.26581217675461 N99A-H256A 1.24613304313073 L106A-I260A 1.21625676502388 V96A-T279A 1.15307066104999 I167A-F285A 1.12409819279997 I167A-M284A 1.11262278234144 S91A-V244A 1.07071226970659 I160A-I292A 1.06977233480876 H256A-I274A 1.0352962537611 Y170A-F285A 1.01057817296003 I136A-V141A 0.991547749004154 I87A-L240A 0.962579143403063 E72A-A248A 0.951339446252656 I133A-V138A 0.938085150627484 M44A-L210A 0.912113026867788 V103A-I260A 0.894165765411966 I37A-V217A 0.859298305805662 I242A-V247A 0.85313127757378 L132A-L280A 0.837235836100019 K81A-F297A 0.837108963626495 I38A-E255A 0.836408159750521 I160A-V291A 0.809529249535586 W237A-I242A 0.800973280631279 I37A-V250A 0.798741181965467 S76A-I87A 0.739245143729499 F78A-V225A 0.718368362842648 M166A-F285A 0.714468992532737 N69A-V286A 0.70494110526896 E72A-L92A 0.671273593721858 G98A-I249A 0.665930674243591 S95A-I249A 0.656528845357494 F241A-I246A 0.65203748923709 F134A-A139A 0.649514250169241 L92A-I282A 0.645603451660707 I38A-G251A 0.642468726872542 S107A-I261A 0.641517708146083 A47A-V205A 0.637024748222389 E72A-V247A 0.628472894019903 V96A-A283A 0.626530203804596 A41A-N252A 0.622164423691175 G45A-F67A 0.613599079126343 L73A-V286A 0.597460434552914 Y43A-L210A 0.592576575021465 V71A-A218A 0.590029270935096 S95A-I282A 0.583246051836525 N69A-I282A 0.580787703198275 S42A-E255A 0.574034551359713 V96A-I282A 0.569677401922485 Y43A-V209A 0.567395028609138 F30A-T227A 0.566965127259504 V110A-I260A 0.563219545450298 A283A-P288A 0.562771810657156 T48A-F67A 0.55521522850805 A135A-F140A 0.552574391352187 F228A-E239A 0.552437873825222 L100A-V276A 0.552001152795629 I87A-E239A 0.551962283798629 A135A-L280A 0.538163652199241 A41A-E255A 0.536046945263453 N128A-V276A 0.527423468873451 Y33A-I220A 0.519778001875589 A79A-I87A 0.516136146632411 L92A-A287A 0.51575933791567 S76A-V88A 0.500987846437294 A27A-T227A 0.495464374420907 A65A-F285A 0.489537651214914 V103A-A259A 0.488536912461888 F30A-L224A 0.485958386423712 L132A-I137A 0.478705755814677 R46A-S258A 0.478621012227347 M77A-L290A 0.477294312799174 H256A-A275A 0.473373154698491 V71A-E255A 0.470255322122346 A70A-T214A 0.468670920961614 V88A-G243A 0.465233790691077 S95A-V244A 0.456792647074759 E72A-C293A 0.456520436035019 P143A-L158A 0.456366071714726 A90A-F241A 0.453815568759369 G94A-I249A 0.453017100094547 S76A-L290A 0.450516638093457 M44A-E255A 0.447317567433983 S42A-S258A 0.447160143323145 A41A-V71A 0.442370107875075 E72A-T214A 0.44126159530121 V250A-E255A 0.440160683664049 M44A-T214A 0.436004369399131 F108A-I260A 0.435477695567876 F78A-S221A 0.434975249426281 S76A-S91A 0.434930283790409 N99A-V276A 0.434508384164399 N99A-I274A 0.432603483736443 Y43A-A213A 0.428233391174331 S163A-V289A 0.426154439696307 L40A-V217A 0.423020014620512 R46A-L210A 0.420737840127268 E72A-T93A 0.419808481782883 P143A-K155A 0.419452851055834 C34A-V247A 0.410705193337391 L80A-F297A 0.409670134412718 I75A-S221A 0.405874366827648 A47A-V209A 0.405304717185922 F140A-A283A 0.404258154642722 I37A-G251A 0.399203574420376 L132A-V276A 0.397486052056613 L92A-A248A 0.396001303068794 Y33A-L224A 0.394909339041055 I160A-P288A 0.394790900456612 L97A-L102A 0.394773454122499 I164A-P288A 0.394238573161527 V71A-N252A 0.392963542884562 S95A-T279A 0.390457804921329 F228A-I242A 0.38999819048872 I137A-I142A 0.389833253769904 A41A-V250A 0.388519459221086 A65A-V286A 0.387817531182292 A65A-S278A 0.38737910379687 A259A-V272A 0.38705237904512 S107A-A271A 0.385950823436476 T48A-A206A 0.383029504789176 I142A-S147A 0.382827491341807 L280A-F285A 0.382816514036685 I74A-A218A 0.379079363577897 I162A-P288A 0.37316920224213 A39A-A253A 0.37122391174893 A79A-A218A 0.37013442266356 S91A-I245A 0.367599769060758 I87A-I242A 0.367505079363629 I75A-V225A 0.366625058846524 I249A-A254A 0.36578711140374 A70A-A254A 0.36491537416003 A41A-A248A 0.361512477198643 A41A-A212A 0.358918385088502 V35A-Y43A 0.356205298661076 V32A-Y219A 0.356090723365416 T48A-T66A 0.352727090907007 M44A-V209A 0.351571600410396 G68A-E255A 0.350289560926301 K81A-A218A 0.350105132423729 A70A-A253A 0.348952365619788 M44A-G68A 0.348799201541485 A41A-A218A 0.348795317932181 Y33A-S221A 0.347690388460562 L131A-L280A 0.347062588089132 N157A-P288A 0.346538213627241 E49A-T207A 0.346382959529543 A254A-A275A 0.343622986382143 I75A-A218A 0.343022416851655 A47A-T207A 0.34112328031734 S258A-A271A 0.339780823639514 A79A-L84A 0.339195251815571 L89A-V244A 0.338554483868379 E49A-T66A 0.337536822332097 A79A-A90A 0.335648847768947 T85A-C293A 0.335004524333504 S163A-A287A 0.334246902235131 I37A-I220A 0.33367972451983 A90A-A287A 0.33236667862402 G94A-V244A 0.329903864696114 G61A-A172A 0.329100588053215 G105A-A253A 0.328345136780493 E72A-Q281A 0.326086118025883 A259A-G277A 0.323619248948114 A104A-G277A 0.322526499749188 L169A-M284A 0.32160769930183 A70A-S278A 0.321603696469277 L102A-A253A 0.321540984952891 L29A-T227A 0.320967702549957 A257A-S278A 0.320335101161923 I87A-V244A 0.319049139754875 I282A-C293A 0.319048013928144 A65A-A287A 0.317391533381596 A135A-M284A 0.316505106975796 E273A-Q281A 0.316187669991047 N64A-H256A 0.315301148756731 V71A-V217A 0.313702859835336 L132A-T279A 0.31349368411086 T66A-E255A 0.312865535698569 G94A-A283A 0.311130107098383 M44A-A213A 0.311074479632345 R126A-L131A 0.310332832010408 A135A-G277A 0.30899268039942 M77A-C293A 0.308714065555385 A70A-S221A 0.307091585955978 T48A-L63A 0.305963468096065 N128A-M284A 0.305065601582251 A79A-E222A 0.304752437866473 L73A-L290A 0.301741686486207 T48A-L210A 0.300735879476307 G45A-S278A 0.299591048738385 I167A-V289A 0.299278227777366 V71A-T214A 0.296411370782086 L100A-T279A 0.29569131023171 T279A-P288A 0.295026324369322 S159A-V291A 0.294606386759183 I74A-V217A 0.294192871950169 A139A-I162A 0.291417442666718 L100A-G105A 0.290941636510954 I75A-G251A 0.288735836124255 E72A-V286A 0.28840204286754 A47A-A70A 0.285981519307887 G68A-S258A 0.285235680798973 I136A-A283A 0.2849362313308 V103A-V272A 0.283884077591437 T66A-V286A 0.283404709637877 R46A-A206A 0.283399895201196 T66A-T279A 0.280074507059794 R46A-N252A 0.279060572071887 T227A-W237A 0.27831066996706 E72A-T279A 0.278256543529051 L92A-A283A 0.278119006563345 C34A-N252A 0.277598283453064 A39A-A254A 0.275960300467056 G243A-N252A 0.275082224917063 A104A-A259A 0.27499549711792 L80A-V88A 0.274425173673397 E72A-N223A 0.273146487951105 S42A-A257A 0.273111813101018 T93A-P288A 0.271419418397123 L63A-G68A 0.270987416926844 K81A-E222A 0.270044465168167 G98A-G277A 0.268675369496736 N69A-E273A 0.268115545915409 Y170A-Q281A 0.266874673986028 Q281A-A287A 0.266327370062198 G86A-L240A 0.266280618482921 Y170A-M284A 0.265354408104322 T214A-E222A 0.260637962241327 G98A-E255A 0.259663992769863 V88A-V247A 0.257882528014723 A90A-I245A 0.257210498313666 C34A-E255A 0.256754331506979 V247A-N252A 0.255395156428101 L92A-V244A 0.255027817740319 A70A-N252A 0.254707858625602 A90A-I242A 0.253711926604462 G86A-S238A 0.253382769173082 L84A-V225A 0.252879085764983 V96A-L101A 0.251701713322277 C34A-E239A 0.250904863371728 I38A-I249A 0.248128962273198 S163A-Q281A 0.247608350562007 V217A-E222A 0.247109662884218 S163A-I292A 0.246445201484903 C34A-I246A 0.244299244998923 G68A-A275A 0.2437066820012 Y43A-E255A 0.243682207925743 I136A-M284A 0.241920022491753 E72A-V250A 0.241911155471444 C34A-V250A 0.241543499007939 A65A-I282A 0.241272439753478 A104A-I260A 0.239558028970086 F30A-F228A 0.239355834386074 N64A-M284A 0.239222117325008 L100A-H256A 0.239087287099028 L63A-N69A 0.238305844074674 F134A-Q281A 0.238196839517867 I167A-P288A 0.23770690901716 R46A-A213A 0.236392650654433 A41A-G251A 0.236029977177265 E49A-L63A 0.235445538155927 S147A-N157A 0.235404509884525 T66A-T207A 0.234445185081173 A212A-Y219A 0.234398104980585 S76A-P288A 0.234312995519505 G61A-N69A 0.233792347338168 A41A-A213A 0.233616019650719 V141A-I162A 0.231023004145614 Y33A-A218A 0.230895631497258 I260A-A271A 0.230815357586224 C34A-I87A 0.230559347003426 N69A-G98A 0.230022566122347 L73A-N252A 0.229916882992002 T36A-L224A 0.227432141560734 S42A-A70A 0.227257308213597 V35A-V250A 0.226396716083929 E255A-T279A 0.226330378934011 T36A-T214A 0.224666997796124 L106A-H256A 0.224606097982331 C34A-L224A 0.223960512199417 T93A-Q281A 0.223498054655284 I165A-V289A 0.223429047400651 L73A-P288A 0.220359841293877 I162A-M284A 0.220188338425551 S42A-S278A 0.219634876055083 I165A-C293A 0.219532414156564 S147A-S154A 0.218996237392492 A104A-V272A 0.218176816987341 A31A-S221A 0.216307643053695 L89A-P143A 0.216083538206002 L80A-S294A 0.215284316909854 N69A-G94A 0.215183603257719 I74A-C293A 0.214743015892005 T214A-E255A 0.214742658570143 S42A-A206A 0.212942303218165 N69A-S76A 0.212921662254077 L63A-T207A 0.212883353234608 S163A-V291A 0.212056517914681 A27A-H226A 0.211686157658293 A259A-I274A 0.211211779406595 L80A-V225A 0.211158036762016 S42A-V209A 0.210840516623608 W237A-I245A 0.210042044316328 G130A-V138A 0.209026082584734 F146A-K155A 0.208738610139614 N69A-P288A 0.208445047000254 A79A-V88A 0.207894498066771 G61A-L174A 0.207834094773443 T227A-E239A 0.207795841364494 L40A-V250A 0.207683460916837 E72A-V88A 0.20690590981071 I74A-T85A 0.206166620816707 A70A-F211A 0.205799431449512 I164A-I292A 0.20376351772958 F108A-A275A 0.203649929114913 S91A-V247A 0.203249456951248 E239A-V244A 0.203219819422892 S95A-A253A 0.202952688698764 T66A-G277A 0.202726475090723 S163A-M284A 0.202721184577575 G61A-F285A 0.201260698577953 Y43A-N252A 0.200562983659603 A70A-S91A 0.200545616088899 M77A-V247A 0.200120370142615 Y43A-N69A 0.20000949271793 A139A-K155A 0.199568453569718 L84A-E239A 0.199568136675484 L101A-N252A 0.199374982886737 P143A-S159A 0.199312298518547 C34A-F241A 0.199188121333714 A39A-R46A 0.198887731098617 L73A-M284A 0.198660051558712 A213A-S221A 0.19854567909756 M77A-F297A 0.198294492841947 N157A-I162A 0.197406538469547 R46A-H256A 0.197347029026268 T66A-I75A 0.196647789225155 K81A-N223A 0.196582400778389 N99A-S107A 0.196129798260919 R46A-N69A 0.195903778571944 I249A-T279A 0.195884407644203 G68A-A257A 0.195636850961663 C34A-H226A 0.195419330004249 E72A-A283A 0.195333982893666 N252A-G277A 0.195033504816078 L40A-N69A 0.194397425144801 S76A-H226A 0.193282611862338 N252A-L280A 0.192764891322244 E49A-A271A 0.192418243949501 I74A-G251A 0.191775944027769 A104A-S278A 0.1908608630831 I133A-V276A 0.189469915317257 T93A-L100A 0.189131753735229 T48A-A254A 0.187353265417914 G277A-F285A 0.186678348094761 V141A-S159A 0.185522187859481 T36A-I75A 0.185118811587627 V138A-F146A 0.184733506330673 I137A-V145A 0.184087491083595 M44A-S221A 0.184085734491502 G105A-H256A 0.184079595050172 A218A-G251A 0.18366984907974 V96A-S278A 0.183161004994417 H127A-G277A 0.179178005699922 S107A-S278A 0.178839495015837 G68A-N99A 0.178527939823664 V205A-L210A 0.178213774361492 T85A-E239A 0.178008517112399 G243A-A248A 0.177059177779865 G61A-M284A 0.176617205912698 I75A-H226A 0.17632405487814 G98A-S107A 0.176318538514439 V32A-S221A 0.176277657174409 G130A-Y170A 0.175597911676886 E144A-K155A 0.17537512851195 T36A-T227A 0.173845451555574 S129A-V272A 0.173693784079802 T93A-A139A 0.173569714187253 N64A-L73A 0.173328762524417 L280A-V286A 0.173223199653036 T85A-T227A 0.172729331544155 A65A-I274A 0.172528519807189 N69A-E255A 0.171959864177066 I87A-G243A 0.171665490476496 I75A-L224A 0.171108711439738 T93A-V244A 0.171089210637393 P288A-C293A 0.170831553366801 V32A-V247A 0.170772205667636 I136A-A287A 0.17062099595895 V138A-P143A 0.170444168049663 E72A-F285A 0.170219252356461 L73A-K81A 0.169857982959207 L102A-S278A 0.169738490391929 N64A-Q281A 0.16940946802356 G68A-F285A 0.169185375279016 H127A-V272A 0.168978921083207 S76A-Y219A 0.168144657821053 L100A-A275A 0.1677549652279 A257A-V276A 0.167485832311672 K81A-L224A 0.1674214983919 G68A-A218A 0.167135974931787 A70A-G277A 0.165485305431662 G130A-A275A 0.165471663852888 A70A-G94A 0.165204347767709 G105A-A257A 0.164677606994708 G68A-A212A 0.164544877042827 I137A-I162A 0.16370755822473 T66A-N252A 0.163681490836575 E72A-V289A 0.163576289635945 V103A-E273A 0.16282069065902 F67A-A206A 0.162482104011916 G94A-A257A 0.162351995648314 L92A-N252A 0.161334536688322 V35A-A253A 0.160852971816309 A47A-S258A 0.160814948790728 V71A-E222A 0.160550900003053 G130A-M284A 0.160353347823586 G243A-A254A 0.160244224885814 M44A-S278A 0.160069557498832 E49A-N64A 0.16000932404227 L97A-V103A 0.159549478358714 T85A-F297A 0.158848358613909 L132A-V138A 0.158777664469998 A39A-E255A 0.157564072883468 L84A-T227A 0.157488713148345 F78A-V217A 0.156953312536906 E49A-N252A 0.156715573251466 A104A-I261A 0.156045585599713 L100A-V272A 0.156041285700243 E239A-A248A 0.155313824287859 L89A-G243A 0.154172050376051 T66A-A283A 0.153913337052179 V96A-A254A 0.15276759324497 N128A-L280A 0.152706126871008 G130A-T279A 0.152407913740276 V35A-S42A 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