[Wed Aug 24 18:22:02 2016] >Input file: TM4M48CGD.pdb >TM segments: A6-26_A44-66_A69-115_A246-268_A272-292_A341-366_A377-397_A399-441_A444-472_A488-513 MAIDEN score = -51.8478 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- R441A-F446A 10 I440A-F446A 1.22651129655235 L49A-I54A 1.21564205781574 I425A-I430A 1.2033874887783 D22A-L350A 1.19146808287257 Y414A-T468A 1.1542258585735 L63A-K69A 1.11296334306744 L81A-K511A 1.07707204625197 I85A-I504A 1.06924028736617 F19A-S354A 1.06474175264738 K69A-A365A 1.03444436274353 Q249A-L254A 1.02202220914953 H66A-D439A 1.02172364955391 F12A-Y264A 1.0123860043409 I72A-I85A 0.991573321558882 V21A-D353A 0.978145051898652 F98A-V391A 0.921999149874705 K69A-A265A 0.910882203697538 L13A-D277A 0.89471230959065 Q65A-A265A 0.864160180048704 E360A-A365A 0.84399997116717 W75A-F82A 0.839978743466453 P55A-P256A 0.825925263817059 F111A-L341A 0.823304576646969 W75A-L81A 0.793074104044246 V89A-F258A 0.792448137912834 D22A-L347A 0.789221604447958 F82A-V507A 0.788514812388438 I17A-D353A 0.784565347961919 Y431A-F436A 0.780921751737212 D11A-V16A 0.772394273516355 I72A-Y87A 0.769748462922002 V90A-I362A 0.763959915843856 W75A-I85A 0.753747727178471 H66A-N268A 0.737912365689342 L61A-V274A 0.73652881622674 V16A-D353A 0.7328426550655 E60A-A265A 0.722275952026333 A71A-K83A 0.719189982660485 L61A-L261A 0.713736836267184 L63A-L262A 0.707191958161227 L23A-L350A 0.705776999277678 L49A-V250A 0.701733146772881 F111A-F344A 0.698188354045707 L61A-L262A 0.69711643888485 F57A-A278A 0.676949355954492 V21A-L350A 0.67252752733499 A424A-W456A 0.671522179255853 L63A-A265A 0.671302365248476 V21A-G351A 0.668801535131903 Y99A-L407A 0.666222626960327 L78A-Y431A 0.665624869706069 F110A-F344A 0.664755583979253 Y95A-L407A 0.660492400166435 L417A-L464A 0.658241315002095 K83A-V507A 0.655622258248845 I104A-L347A 0.655438361082012 F82A-I504A 0.654141647325126 V96A-A412A 0.65123708702791 V90A-A365A 0.647905170637711 W75A-G86A 0.642380602126108 V89A-A419A 0.634933144654648 A71A-V90A 0.629410520027636 I54A-S282A 0.627850550532608 A88A-V502A 0.620290693636575 A424A-P461A 0.617300518066849 G52A-F463A 0.612996319255741 F57A-G255A 0.612475067156712 A424A-W429A 0.60951672027695 F421A-A460A 0.608394154666553 F82A-A426A 0.60349238179972 A93A-F258A 0.599373191095741 A71A-Y87A 0.598474698168007 W6A-F12A 0.597332446106678 A71A-I85A 0.596263632431169 C74A-K83A 0.594802488323551 Y58A-V274A 0.589687559409906 V260A-A265A 0.586156791818137 F251A-P256A 0.585864414000079 G64A-Y264A 0.581336128578201 R409A-G493A 0.580247869723049 L91A-S500A 0.573826690310701 V21A-N285A 0.57261925478973 R409A-I496A 0.570750906776731 I103A-R109A 0.569930005265785 A419A-V501A 0.564729291531899 L61A-Y264A 0.563625203367318 A88A-M503A 0.563084782048176 Y45A-A247A 0.561942205057331 F403A-D408A 0.561574621875556 F421A-P461A 0.555684420909645 Y410A-W494A 0.554337490335541 I54A-A278A 0.543641587986868 F57A-P256A 0.542845491037924 L63A-C74A 0.541826441197408 L465A-Y470A 0.541318058232978 V90A-F258A 0.532962139013696 L56A-P256A 0.532736618451974 F19A-L350A 0.529278862193931 Y58A-Y275A 0.529094289177924 F19A-L254A 0.52684488778002 F258A-A361A 0.526388850255334 A247A-F463A 0.522456319479226 D97A-F385A 0.521853575127583 T248A-I467A 0.518998736473894 Y410A-I496A 0.51882804924765 K69A-A263A 0.518632618338819 K83A-M503A 0.515689502222102 I430A-R435A 0.515051736845913 M48A-A246A 0.511767046572675 F57A-L254A 0.507713888399512 Y45A-V250A 0.507054672235842 A62A-V274A 0.50619158765365 A71A-T364A 0.505671744717008 F57A-D277A 0.505210166162035 F110A-L341A 0.504640163760841 V89A-A361A 0.501545324925948 P44A-L49A 0.499781329278984 E438A-F446A 0.499667502505071 D439A-F446A 0.492528039576765 F82A-A508A 0.49242737641758 A394A-F403A 0.489529559589138 I17A-N285A 0.488840590199937 F19A-G357A 0.487046415545779 S437A-F446A 0.486618259277326 V427A-T433A 0.484729609663646 V16A-T281A 0.483997108216456 S428A-W456A 0.483334089109838 I425A-R457A 0.479564348075045 P80A-E423A 0.479367905841764 F421A-L464A 0.474703428979978 K83A-A508A 0.47454554003904 L108A-M345A 0.474121419640352 I54A-M59A 0.474073218381377 V89A-S500A 0.473216983441455 K83A-K511A 0.472424907398042 V90A-G259A 0.471920176179475 Y95A-A412A 0.470046682613248 E60A-C455A 0.467915977984558 V51A-A460A 0.467194595845329 V381A-S386A 0.463924486499455 A24A-S253A 0.46384795594897 L81A-V507A 0.456935030449383 G52A-V459A 0.456395589090223 P55A-E423A 0.455391380506861 V418A-E423A 0.454952731366111 I85A-S500A 0.452493720202413 V96A-A411A 0.452273656099426 W6A-N268A 0.450180473340664 V10A-S15A 0.448472923025789 S415A-S500A 0.44658063651689 F12A-I17A 0.445562758421837 K9A-L14A 0.445316560976246 T73A-G86A 0.443881846164529 K83A-I504A 0.438319971997646 L108A-F344A 0.437902644556059 V102A-S395A 0.434305695207494 T433A-Q453A 0.433139280409869 S395A-F404A 0.433037032076746 P448A-Q453A 0.431108395693187 Q453A-F458A 0.429638900019489 I92A-S500A 0.429132117830051 E60A-V260A 0.426751106306858 V427A-R435A 0.426387884459142 S395A-Y402A 0.421668820137848 Y99A-S395A 0.420349840014668 K9A-N272A 0.419299478456608 I85A-M503A 0.418812787985956 I284A-S289A 0.418072048486499 F19A-G351A 0.417703055381898 Y45A-A50A 0.415971488379105 V427A-R457A 0.41166270534901 A412A-A497A 0.407817440883997 G52A-V250A 0.406956166638281 I47A-G52A 0.401489020776761 V427A-W456A 0.400855570514612 F19A-S355A 0.400435118401808 I416A-F421A 0.399303658624738 V21A-S253A 0.399256674573206 V90A-A361A 0.397807580912218 H66A-S437A 0.396916362801517 Q65A-L262A 0.396755199897703 F258A-I416A 0.395360046940068 I92A-A412A 0.395078027811917 Y100A-S355A 0.394787510681531 Y99A-F404A 0.394710495028486 V16A-L261A 0.394193361462191 A71A-L262A 0.393607763007411 A88A-A419A 0.392257032890824 V79A-A426A 0.390683846807312 I104A-M345A 0.38812943344547 G84A-M503A 0.388039354401191 V420A-L464A 0.38697408826123 A71A-V89A 0.386712733414829 N101A-W106A 0.386352518600925 G84A-V501A 0.384231467173143 S15A-Y264A 0.384122000348697 D97A-F389A 0.384101432505232 L14A-F19A 0.383858287484356 M48A-A247A 0.382377650197643 V96A-D408A 0.381749514079207 R377A-A382A 0.380156660895609 F12A-D277A 0.379831766413641 Q249A-Y470A 0.377826873879837 L23A-L347A 0.376928350687236 A247A-F466A 0.376665693111274 M48A-W456A 0.376635963537704 M59A-W75A 0.376370064144592 I17A-T281A 0.376305749557492 F356A-A361A 0.376125018504382 Y99A-D408A 0.375314790662937 I104A-G351A 0.371318307108949 V21A-S354A 0.371091931574949 L407A-A412A 0.370719445135725 A88A-A506A 0.370546473336268 P55A-C455A 0.369718823747059 Y410A-S415A 0.369627713727018 H405A-N490A 0.368788930779507 A71A-A93A 0.365743208280358 A263A-E360A 0.363176047022876 L63A-S266A 0.36237775467767 V507A-L512A 0.362140741545705 A50A-A246A 0.361785301821685 T73A-A365A 0.361564749782061 Q249A-P256A 0.36115676227599 A71A-A265A 0.360029325242488 P256A-S282A 0.358663875408013 Y99A-G399A 0.358497731522317 G64A-L261A 0.357454709549771 W452A-V459A 0.356765493795535 V16A-Y275A 0.356655476178944 Y58A-V454A 0.356093909433775 A424A-R457A 0.354115250282778 A62A-D439A 0.352934701867373 V96A-Y414A 0.351599700805268 F19A-L352A 0.351469483167653 Y87A-M503A 0.350759150503326 A265A-A278A 0.350533208156191 Y100A-D408A 0.349349096535363 A20A-A287A 0.349001357569059 S286A-I291A 0.34762053549988 L406A-A411A 0.346731927879969 G53A-P256A 0.345966617987433 G64A-A263A 0.343850629785726 A88A-S415A 0.343560618316973 G255A-Y264A 0.342960834166099 C74A-V79A 0.341167587166909 L417A-T468A 0.340924605530153 N101A-R109A 0.340011500439003 C74A-F436A 0.339245679759422 M59A-V250A 0.336433635386711 I425A-Y431A 0.334904054500599 A419A-A497A 0.334085929337034 Y58A-L63A 0.33293636414586 I72A-G84A 0.332808045995844 D97A-V390A 0.332650845657403 G76A-L81A 0.332032973776795 W6A-D11A 0.331180280242672 A88A-A361A 0.330810171583763 A20A-G358A 0.330517007629172 G257A-A412A 0.330186802876411 A50A-P55A 0.327066739292957 G76A-A508A 0.326098620956488 G259A-I362A 0.326048041991704 M48A-G53A 0.325997936180817 P448A-W456A 0.325377815088387 L91A-V96A 0.32530187271343 V96A-N101A 0.325100604590914 G257A-I416A 0.324655728947 G76A-A365A 0.324227942997455 M48A-W452A 0.323821865268838 Q65A-Y264A 0.323758787090862 W75A-M503A 0.322990397336942 A419A-G498A 0.322240122758579 A20A-S354A 0.321899543102903 I425A-W456A 0.321869000734655 A88A-S499A 0.321704838636598 D97A-Y388A 0.321619002414685 A424A-A460A 0.320958509417244 W106A-F111A 0.320577226346909 G53A-F251A 0.318048007536814 V96A-L407A 0.317110179294623 G357A-A365A 0.316275554909863 Y45A-Q249A 0.316197680818354 L261A-E360A 0.315652836958043 G70A-G358A 0.314758101252559 G84A-G259A 0.313633125314216 G255A-A412A 0.313379329506427 V21A-Y100A 0.313298999416802 I17A-I284A 0.313016078122636 L262A-E360A 0.312199296650848 A62A-G257A 0.312087465794714 G84A-G257A 0.311568176389453 G498A-A506A 0.311174380771797 E423A-F463A 0.310448554585312 L108A-A348A 0.309038059339299 M503A-I509A 0.308285933313886 Y58A-V250A 0.307837874917521 I92A-F258A 0.307673193261877 M503A-A508A 0.307465961916855 L262A-A361A 0.30586771970821 A20A-D353A 0.304463963635243 D439A-P447A 0.30354680948738 Y100A-R409A 0.302077798430259 A88A-I416A 0.301733454166379 V89A-Y410A 0.301718350800096 F19A-F356A 0.301676034829333 V89A-G257A 0.301208482036433 G18A-A24A 0.300447618833287 R109A-C396A 0.300264750772545 A88A-A365A 0.299296834925734 F98A-G392A 0.299084140626063 E360A-L366A 0.29870877094538 E378A-L384A 0.298446483610229 S15A-G351A 0.298355586780923 S107A-F344A 0.2981597569095 D439A-I444A 0.297648605614176 I92A-D97A 0.296867753401321 L417A-E423A 0.296000768862965 V16A-D22A 0.294990976854002 G52A-S253A 0.294107959446074 P55A-T433A 0.294018449057768 A265A-A361A 0.293231783850985 K69A-R77A 0.29269388863467 M59A-Q453A 0.292619599696856 N25A-F290A 0.29224434781286 P80A-K511A 0.292229281521795 P256A-Y264A 0.291831001574492 A265A-K276A 0.291733619624954 S395A-F403A 0.291042265660716 P256A-T281A 0.290852816927365 G70A-L366A 0.28998825028525 L492A-A497A 0.289614599535751 F343A-A348A 0.289064180038217 G18A-L347A 0.288625626687574 L14A-D353A 0.287828798061125 L261A-A361A 0.286746686420821 G46A-S282A 0.286480217774139 A394A-G399A 0.286229114204863 G86A-L262A 0.286074341422476 V260A-D353A 0.286009272901583 G255A-N285A 0.285586860014565 I440A-P448A 0.285435291054029 N101A-G358A 0.285286195493113 G18A-N25A 0.285213001321386 Y87A-G358A 0.28455171074061 G413A-S500A 0.284296207755764 G18A-S354A 0.284158375786848 Y388A-L393A 0.283314134222022 G18A-S282A 0.283185800684169 I440A-P447A 0.282795737122176 L78A-A426A 0.282182803663892 A71A-A419A 0.281579629376767 P80A-A508A 0.281536164556796 A62A-L262A 0.280334961084764 Q65A-E438A 0.280238766232015 L108A-A113A 0.280036980009641 W106A-T397A 0.279407960645024 N25A-G351A 0.279230243320739 E438A-G449A 0.279072384752635 L61A-G70A 0.278748752045349 N272A-P447A 0.277859599499483 L49A-A247A 0.277022560787051 G53A-L254A 0.276907464485088 V96A-D353A 0.276828102813492 G64A-N273A 0.276384234297693 Y95A-D408A 0.27497066774691 V10A-V16A 0.274556220186594 E423A-N434A 0.274255845653872 S107A-M345A 0.272234314331019 S107A-L347A 0.271631083598582 S395A-F401A 0.271410861669726 G8A-S15A 0.270569122761299 A411A-W494A 0.269529093729089 K9A-N273A 0.269224011539679 Y99A-F403A 0.269080599830357 N268A-E360A 0.267705947651515 I440A-G445A 0.267539821725949 G84A-A365A 0.267532112128167 V79A-V427A 0.267487805376293 G399A-L406A 0.266293731925518 G64A-A365A 0.265207478182107 V51A-C455A 0.264906194374733 G18A-L261A 0.264760731251621 A426A-R435A 0.263705632291395 C74A-E360A 0.263154295068047 L81A-I504A 0.262862025674124 R441A-P448A 0.262841717617726 K69A-T364A 0.262587008481883 A20A-A278A 0.262428436889065 V89A-I416A 0.262263196411949 Y58A-P447A 0.261673934793717 Y58A-D277A 0.261437441166284 Y95A-V390A 0.260716053075462 T73A-K83A 0.26066673932935 G413A-L464A 0.260469450012746 L406A-G493A 0.260325334258255 P256A-V420A 0.260278592041819 V420A-C455A 0.259115816093269 F422A-C455A 0.258439482916646 W75A-G259A 0.256969007310629 K69A-Y264A 0.256646358219022 G70A-L78A 0.256234298722091 S107A-L341A 0.256149136351031 L23A-S253A 0.25604894952728 G255A-A419A 0.255564907029918 S386A-V391A 0.255404437015142 G493A-S500A 0.2553730432482 H66A-A265A 0.254928527340562 W75A-G84A 0.254141781287743 G52A-A424A 0.253483464264351 V454A-A460A 0.25339462225941 I72A-L366A 0.253374123918031 K69A-Y267A 0.253231545785825 A62A-R435A 0.253150729202406 V16A-Y267A 0.250595490812913 G259A-S354A 0.250209817306763 Y431A-D439A 0.249929515075238 Y95A-S500A 0.249782448979121 Q65A-Y267A 0.249769614080504 L78A-G84A 0.248050554384077 F19A-G259A 0.247934565635044 D11A-G18A 0.247784540055649 A93A-G413A 0.246850477758993 Y275A-L280A 0.246711604986462 S415A-V501A 0.246212891266396 Q65A-N272A 0.245813062107171 T288A-T349A 0.245806643882506 Y414A-A497A 0.24572113724766 Y410A-V418A 0.245601184274372 N434A-Q453A 0.244977075570525 Q65A-G259A 0.244922633272094 W106A-G351A 0.244814947928207 S15A-G358A 0.244363411502285 A71A-A361A 0.243621232020992 N25A-Q249A 0.24239514352266 T73A-L78A 0.241969740941927 G70A-W75A 0.241704787504169 N490A-G498A 0.241499419485883 D22A-Y100A 0.241344247691348 Y95A-G357A 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