[Wed Aug 24 18:46:28 2016] >Input file: TM4N4WATD.pdb >TM segments: A231-254_A263-286_A312-340_A356-378_A399-424_A450-472_A516-538 MAIDEN score = -79.3149 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C273A-A324A 10 C469A-E518A 9.27223828536887 H470A-N521A 1.2248876265766 I266A-L335A 1.12917269770308 Y322A-T372A 1.07234763483301 W281A-I317A 1.0161886159959 V276A-I320A 0.915559516495814 I234A-W281A 0.885606968027623 W339A-L452A 0.848853150564036 V270A-F332A 0.835499691811921 T241A-F274A 0.824341002460211 C273A-L325A 0.817553024313368 V270A-W331A 0.81562457606962 T248A-F268A 0.810373989089946 T466A-N521A 0.785230780861162 W331A-F360A 0.777891013337616 W339A-L419A 0.754181168192806 L249A-F268A 0.746326023699915 V334A-F360A 0.743886105706196 W281A-V321A 0.742334374105316 F462A-M525A 0.734415271978209 T241A-F275A 0.728214666729218 T241A-I530A 0.720152529397385 F455A-M532A 0.71121635235477 L246A-F275A 0.693744213129603 L405A-F467A 0.691535675592267 Y337A-L419A 0.687671593581827 C244A-I530A 0.682858859863635 F237A-F526A 0.678578876533344 F274A-A324A 0.676323562158189 F252A-F268A 0.662969276197638 W339A-F418A 0.659817861849358 C469A-V517A 0.650852824865616 C244A-T534A 0.647230474944738 I266A-W331A 0.645676113101657 G415A-F460A 0.627540181289123 G416A-F460A 0.626736468302668 Y323A-F369A 0.625595864872166 G242A-F274A 0.624270214411764 V270A-A327A 0.62244010107036 F332A-G415A 0.618016064395573 F332A-G456A 0.617869892834505 F462A-G527A 0.612531648183297 F455A-W535A 0.586908271681029 V319A-A324A 0.585888243932362 Y337A-G415A 0.58572169539264 T245A-F274A 0.585360270868844 A327A-W365A 0.581061550422244 A401A-F471A 0.576019568056239 F455A-A531A 0.5746774192692 F274A-L325A 0.566565164646354 Y269A-W331A 0.563840052021634 T248A-T534A 0.561893832878299 G238A-F526A 0.557531558892967 T251A-V536A 0.555043720496355 Y233A-K519A 0.55486341395815 I408A-V463A 0.550626426846291 A459A-M532A 0.549723339904122 A235A-F285A 0.549539877693485 C244A-C273A 0.533441012778643 H340A-V423A 0.53268400920785 C244A-S533A 0.532065018544998 L419A-F457A 0.530415824359232 V404A-H470A 0.529491786866997 N271A-G529A 0.526304527615347 P263A-L335A 0.525482047600459 T245A-I530A 0.514637805458459 F332A-A459A 0.511587031307772 T245A-N271A 0.511096744824695 V319A-T372A 0.507550041883459 F462A-T528A 0.502620178613856 W331A-T364A 0.499903593227959 V463A-A524A 0.495926620655108 V270A-G529A 0.492938525303677 F237A-F523A 0.488889564188122 L249A-A272A 0.475925085847088 V240A-I530A 0.475917764818709 S278A-V321A 0.475221790388752 V330A-T364A 0.466575384610518 C469A-N521A 0.464640774173583 L267A-S533A 0.4638425137248 C469A-I520A 0.452361609926118 I234A-L282A 0.452212155637384 P263A-K356A 0.443448718163837 L405A-F471A 0.431344113594373 H231A-F285A 0.427383951607048 G328A-T364A 0.425804671880384 T241A-G527A 0.420901501843757 C244A-M532A 0.419279121717049 M525A-I530A 0.419216918824574 Y399A-V404A 0.414907302556992 T336A-G453A 0.414007242542398 F274A-F526A 0.408795170902173 R400A-H470A 0.408005666191584 G409A-F467A 0.407568298474707 L267A-W331A 0.404848390460078 G242A-I279A 0.40220716510235 L371A-F403A 0.398865828010718 V334A-G415A 0.395914451928556 L412A-F460A 0.395275192971398 V333A-V411A 0.39371335242385 I375A-F403A 0.392416616743958 V270A-V329A 0.389515108340022 H340A-R421A 0.388099455736291 I465A-A524A 0.387666664519682 V411A-G416A 0.387322481525951 H231A-M286A 0.386046445280277 A327A-T364A 0.385847964250706 Y269A-G328A 0.384102652611219 V315A-L376A 0.380621407692953 L412A-Y417A 0.377064054785343 V329A-V463A 0.373027362734287 I234A-L522A 0.372968469421602 A239A-L282A 0.370808980699097 L458A-T528A 0.365968259897484 T466A-L522A 0.36482160586599 C469A-F526A 0.360255877152152 A235A-L282A 0.359570710710847 I234A-K519A 0.354228203986143 G238A-I279A 0.352311162567801 Y233A-G527A 0.351851594753176 I408A-F467A 0.348786511274513 L412A-L464A 0.346722677418096 H340A-L419A 0.346196643036827 V333A-L412A 0.345493665293959 L405A-H470A 0.343073300746523 G277A-V321A 0.34166640702429 Y472A-V517A 0.339431139382876 L458A-A531A 0.338731474793384 A327A-L367A 0.337901374908911 T245A-A272A 0.337596312446061 F274A-G328A 0.334588526143476 A239A-S278A 0.33423270582046 L267A-W535A 0.33410099873112 G238A-W281A 0.333932810170895 I266A-V334A 0.332573168536609 A235A-G280A 0.330036971471074 W331A-P368A 0.328441501174694 A324A-G529A 0.327604588331879 I465A-I520A 0.324755588367559 T364A-T372A 0.322849778445549 A239A-G529A 0.322328698787204 C273A-F318A 0.318561954773912 Y233A-Q284A 0.317595996453808 L243A-F275A 0.316983308174066 C273A-H361A 0.315449928850127 W339A-G422A 0.314379046821246 G328A-G456A 0.313667234535473 P263A-W331A 0.3129104996122 G328A-G415A 0.310999511821121 V329A-I408A 0.308533911491998 T336A-G416A 0.306266326503533 W365A-V370A 0.305740218387566 C273A-M326A 0.305200695839369 G280A-C314A 0.304240945740375 H231A-Q284A 0.304150219905077 L246A-N271A 0.301347760697521 L335A-L452A 0.300138251573666 A239A-G280A 0.297041507558342 A264A-G453A 0.296054669688308 A264A-L452A 0.292363656262527 G409A-G461A 0.292033098596576 G461A-G529A 0.291991767634252 F318A-Y323A 0.291207775649015 T245A-M532A 0.290408836634647 L412A-V463A 0.288325211885212 S278A-G527A 0.288168736522015 L267A-M532A 0.285734949128704 L246A-A272A 0.283478434706633 M326A-I408A 0.282412059790047 G277A-G527A 0.279262758854923 A459A-A531A 0.277942499411427 V276A-V321A 0.276797322624219 N271A-M326A 0.275291709724741 L419A-G456A 0.273235757696245 C273A-G280A 0.270397007811471 V330A-L363A 0.269683741865336 L267A-L335A 0.268583105160851 G280A-F318A 0.268283460530792 G238A-L282A 0.265881045418862 C273A-V334A 0.265205213541223 G328A-L363A 0.264417875892765 T466A-M525A 0.261950492178467 I234A-A239A 0.260898767817223 T248A-C273A 0.258270347307702 V333A-F418A 0.257898268159941 Y233A-V517A 0.257483380175228 M326A-P368A 0.256695456120396 G456A-W535A 0.255530876246131 N271A-G527A 0.255350168263937 G461A-N521A 0.254544221908375 G453A-W535A 0.252398884905592 V270A-M532A 0.250635819746472 S468A-K519A 0.250597108816114 I279A-I320A 0.249919444612479 A235A-W281A 0.248749769926912 G328A-G527A 0.248332436632067 Y269A-V536A 0.247383669110178 A239A-A283A 0.246448677139515 L325A-P407A 0.244109963901995 F332A-G453A 0.244044412586875 V463A-M525A 0.242979091597077 L452A-T534A 0.241994717356376 L249A-T534A 0.241642346046222 Y337A-R421A 0.240837090460822 L243A-S278A 0.240309564327394 L335A-T364A 0.239905401119864 G453A-G461A 0.239412767963906 L325A-T372A 0.238428228719128 N271A-S278A 0.236626810233849 V329A-V411A 0.236413453848167 T241A-F526A 0.236179692631627 L267A-T336A 0.236048104458811 P368A-P407A 0.235260990313449 A459A-S533A 0.234946739958461 G280A-V321A 0.234301977574495 P263A-H340A 0.232393027642958 L246A-T534A 0.231304373753345 F275A-G529A 0.230660438489711 P368A-L410A 0.229483563024703 L267A-T528A 0.228470859944541 G456A-G529A 0.228313027339211 C273A-F360A 0.22781413364518 F247A-Y269A 0.226756302536305 V240A-I279A 0.224721898420793 L363A-P407A 0.2243685733161 P263A-W535A 0.223892046570515 F460A-A531A 0.223689441802367 V270A-G456A 0.221258256988364 T357A-W365A 0.220565127561571 W339A-G456A 0.21971774651381 T241A-S278A 0.21951588994819 V334A-G416A 0.21948841740312 L522A-G529A 0.218289927829615 V270A-G328A 0.217260476211278 A239A-G277A 0.21623516067331 F252A-V265A 0.215578563757889 G242A-A283A 0.215031385143257 V319A-V373A 0.214154596617712 I234A-G280A 0.214057755166624 T336A-L450A 0.212885103288203 A239A-F274A 0.212484159792078 V240A-F523A 0.212187099761222 I234A-C244A 0.211392218492007 L458A-T534A 0.211353471989285 Q284A-F318A 0.2105337838428 T245A-W537A 0.210384524129533 V333A-L419A 0.209473012010893 V404A-S468A 0.20875836865638 V463A-G529A 0.208690508529161 V423A-G456A 0.208238856586687 T241A-L522A 0.207827157943125 Q284A-C314A 0.206860347142672 F460A-G527A 0.20674416299985 G409A-F460A 0.206557051722289 T466A-I520A 0.206346478970954 V329A-L412A 0.206031624105572 L249A-V536A 0.205884631831504 A250A-F275A 0.205811230321407 G328A-T336A 0.205216856911242 V378A-F403A 0.204668022500673 G277A-Y322A 0.204411771226159 I266A-G456A 0.203791458120553 V270A-F455A 0.203102138722085 I266A-T534A 0.202641958583021 V276A-W281A 0.2022600470758 I408A-T528A 0.201835368792234 S232A-S278A 0.201518133776902 H361A-P368A 0.200601384154199 V276A-M525A 0.200136400359336 T364A-I408A 0.199633354496924 G277A-Q284A 0.199394745551793 T248A-V536A 0.198045113078524 P407A-H470A 0.198009213440668 Y417A-G453A 0.196858120843494 F462A-A524A 0.195500304224371 G409A-I465A 0.195080593278494 G461A-A531A 0.194837194908799 V265A-C273A 0.194225173542552 L246A-G277A 0.194121508123031 M326A-V373A 0.192546756394817 F467A-V517A 0.192040805083943 C273A-P368A 0.191778322356847 V373A-V378A 0.191748869660719 I234A-G242A 0.190662899087377 S278A-L325A 0.190127924381604 T336A-G422A 0.190008453174151 C273A-V333A 0.188420677498129 P263A-G422A 0.187715899767934 Y269A-W365A 0.187704998495415 A324A-T528A 0.187307824414221 T245A-A324A 0.186993039142985 L419A-G453A 0.186848089613313 F332A-F460A 0.186180080726441 A250A-T534A 0.18597978071289 Y323A-L371A 0.185349487474494 Y337A-M424A 0.185078900561365 C314A-L376A 0.18449024445613 R451A-A531A 0.184295449395997 P263A-S533A 0.183645918060915 V240A-F274A 0.183567395595535 G402A-F471A 0.183565258685871 T336A-G461A 0.183385739298568 V404A-M525A 0.182420665329355 F275A-T528A 0.182017110141586 R451A-W537A 0.181146262292004 G280A-M525A 0.181124195853419 T336A-T357A 0.180427153378022 W331A-T357A 0.180095565675735 W281A-I316A 0.180089189873722 P263A-Y359A 0.179894828385068 L412A-G456A 0.179739908644757 L335A-K356A 0.179513721392356 G328A-L367A 0.179435139155583 A327A-I408A 0.178003947981709 G280A-M326A 0.177866347400671 L335A-G416A 0.175902215457846 V330A-L367A 0.175739286121066 V270A-L325A 0.175603881086149 P263A-L450A 0.175398812136393 W331A-W365A 0.175282786920339 T528A-W535A 0.174916158875268 F318A-V373A 0.173836034363202 V330A-V411A 0.173806226330193 V333A-H361A 0.173567588044591 T248A-T528A 0.172500748672023 Y269A-Y323A 0.172189654717655 V276A-V329A 0.171952000784322 H470A-F526A 0.171599979568893 W339A-G416A 0.171029454798618 M326A-G402A 0.170949954476449 W331A-K356A 0.17050999413602 S468A-L516A 0.169767648988184 C273A-I530A 0.168917007139081 M326A-G529A 0.16834226314813 G422A-I454A 0.168082287230551 A264A-G328A 0.167579434123043 C244A-Y269A 0.167338148225206 Y322A-G402A 0.166768829909278 L246A-V276A 0.165781481135349 Y233A-S278A 0.165677853398468 V330A-V370A 0.165485653396992 L405A-N521A 0.165391718165695 V240A-V276A 0.165290815959426 A235A-G529A 0.165226808992595 G280A-S313A 0.164777474249984 G328A-A338A 0.164036247022643 A254A-V265A 0.163952678258766 A250A-G529A 0.163615767162471 A264A-T538A 0.163172539542989 A406A-G416A 0.162137530858758 T336A-T528A 0.162070939751664 V333A-G415A 0.162004533270045 A327A-T528A 0.161719565513764 A324A-G527A 0.161636831452177 G422A-A459A 0.161636831452177 L243A-G527A 0.161480175115092 L282A-C314A 0.161412763571382 L335A-G456A 0.161377812728375 G238A-M286A 0.16137378306768 T336A-T364A 0.161274379170842 F332A-G461A 0.160175250798447 L246A-W537A 0.160030866781655 H340A-F418A 0.159459839991646 C244A-F275A 0.158394896832339 F332A-V411A 0.158173219182794 F268A-T534A 0.158043356315256 C244A-G527A 0.157918139083753 I234A-N521A 0.157482505209094 W281A-K519A 0.156809120256384 V330A-A406A 0.156149057137211 Y322A-S366A 0.155698484235589 A401A-Y472A 0.155215207894174 F332A-F455A 0.155105347031163 G242A-G527A 0.15468231780019 G456A-T534A 0.153042356985211 I279A-M525A 0.152553087656698 W331A-T528A 0.151364232046807 K356A-T364A 0.151002309427304 S278A-Y322A 0.150349100891985 T241A-T528A 0.150322863053427 I266A-A459A 0.150009074980352 A264A-I454A 0.149897641986656 C273A-L335A 0.148718125404082 V333A-F457A 0.147650076570423 M326A-R400A 0.147495942315759 A264A-M532A 0.146787058033631 G277A-L325A 0.146594905656412 A374A-G402A 0.146512986069807 I266A-V329A 0.146329939281523 G242A-T534A 0.145751722369161 L335A-Y359A 0.145621243766289 C273A-G527A 0.145606230463975 T245A-T538A 0.145580203182531 V240A-S278A 0.145577426843887 Y269A-L362A 0.144937241946529 V463A-T528A 0.144742348626052 S232A-E518A 0.144315498390205 W339A-L458A 0.143817996043041 A264A-H361A 0.143125920666043 W331A-S366A 0.142940652332995 Y337A-I454A 0.142595939771838 L458A-V463A 0.142201696314974 G280A-M286A 0.142056475033776 V373A-F403A 0.141755221715627 M326A-A406A 0.141655740495704 L325A-M532A 0.141258200478211 R451A-A459A 0.140959493834133 V333A-I413A 0.140540577366403 W281A-Y322A 0.139324037320748 A338A-V423A 0.138532935269471 T245A-I266A 0.138384072722121 F460A-C469A 0.138142296278723 F462A-F526A 0.1378162357779 A531A-T538A 0.137707393958763 Y399A-I408A 0.136785992466454 I320A-I375A 0.13623071581764 G238A-C273A 0.135738165981405 V378A-V404A 0.135062143546508 S232A-W281A 0.134793162112838 I234A-L243A 0.134697227199905 T251A-N271A 0.134006663741997 Y269A-Y359A 0.133482819417432 A374A-R400A 0.133278698833467 F252A-V536A 0.133235181672444 V414A-V423A 0.133018741497257 I279A-Q284A 0.132898645004398 C273A-G328A 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