[Wed Aug 24 18:49:02 2016] >Input file: TM4N7WATD.pdb >TM segments: A61-83_A92-108_A122-141_A154-174_A190-209_A215-242_A248-269_A273-293 MAIDEN score = -63.0175 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- Y235A-C248A 10 W236A-C248A 2.42049057463223 K268A-P273A 1.54657147187306 N227A-H286A 1.52787054403675 H71A-V255A 1.32763572220415 I125A-E254A 1.25690933162685 L231A-I253A 1.18969718298442 L161A-I166A 1.11098585369049 K268A-L274A 1.10039001564467 Y235A-A252A 1.05060554942897 I201A-A280A 1.0472092527517 V99A-A263A 0.992424913712217 A79A-V97A 0.928937691621481 I160A-V165A 0.882304961563605 A264A-P278A 0.874040301976889 L98A-T141A 0.823353215588885 L82A-H226A 0.807773139820489 G234A-C248A 0.798268542887145 I220A-L281A 0.791147806127963 V134A-I160A 0.782088455167462 L92A-L274A 0.774153661349284 W80A-V97A 0.766427322523694 I224A-W285A 0.766263940800171 V64A-I125A 0.745234544659692 I74A-S233A 0.736743677306448 I201A-S283A 0.732357243926011 S208A-L274A 0.731993882149745 L70A-V255A 0.731158485872933 V134A-L157A 0.724859040332615 T106A-V123A 0.709307066526332 T68A-I125A 0.668957847385197 V102A-L262A 0.663715539877932 A83A-V97A 0.649792724663368 V223A-F282A 0.639747365363397 Y235A-T250A 0.633885457931905 V223A-W285A 0.630009150325228 P63A-L251A 0.626411781968575 A67A-I125A 0.623313080406559 A67A-E254A 0.623295733400177 V102A-A136A 0.621206962329353 P76A-L98A 0.614684216072971 L231A-V255A 0.613511153962909 L266A-A276A 0.613407982186498 V99A-N259A 0.605203724362242 W236A-A252A 0.596321029218648 A103A-T130A 0.592897097044187 G267A-P273A 0.589480780118411 V204A-L277A 0.587705768446282 P76A-S101A 0.587297892184182 L92A-A275A 0.578371109668325 L70A-S233A 0.57186220398425 L92A-P278A 0.571554690478316 G234A-R249A 0.569651022884109 S104A-I125A 0.559961918121041 G105A-S126A 0.559585532869674 V134A-L161A 0.557072270211188 S260A-F282A 0.531565547188448 G261A-S283A 0.528721714349192 P138A-L157A 0.523255687210657 S108A-S122A 0.521428938256362 V205A-L277A 0.519801155189197 V205A-A280A 0.51523353849561 S108A-I253A 0.502648378185238 W80A-M96A 0.498698203795487 L231A-M257A 0.494125223516958 A94A-T141A 0.493599281569899 L231A-E254A 0.492602411178334 I74A-G230A 0.491389483502802 I197A-G261A 0.489312369782668 M75A-I229A 0.478503774474555 A66A-F241A 0.478461806399601 G234A-I253A 0.476426998192984 M96A-A275A 0.462995200785329 S159A-V164A 0.456452509512395 Y72A-A136A 0.456108042812894 A103A-T137A 0.454319259054265 P63A-K239A 0.443220291526598 A83A-M96A 0.442113274846728 V102A-S260A 0.441964559890501 K268A-P278A 0.440355850499401 I201A-L281A 0.437353565452816 V99A-A264A 0.437233819466735 S108A-M257A 0.431840626028796 T130A-E254A 0.430764773916877 A103A-V132A 0.430432204774454 P76A-L140A 0.430196681786883 A103A-S126A 0.428614179227091 I220A-F282A 0.426388609265586 F135A-L157A 0.425629500256224 H71A-H226A 0.423915015903496 S122A-L251A 0.421459953436694 M156A-L266A 0.419619997762714 V64A-T124A 0.419306513312137 S159A-L266A 0.418816625537872 W80A-S93A 0.413106934729374 A263A-A276A 0.412110739097786 A79A-V99A 0.41180623501536 S101A-L140A 0.410073959875455 H71A-Q258A 0.3993550073909 V219A-L281A 0.389002682543464 L77A-V97A 0.387223149496009 V204A-A264A 0.387058702294141 S104A-T130A 0.386573660636065 H226A-S289A 0.385978886191021 N227A-W285A 0.382365489868733 V99A-F282A 0.377342605471757 H71A-N227A 0.365988708460423 T78A-I229A 0.363773565593124 G100A-Q258A 0.363579715970779 S233A-V255A 0.363398005694283 A264A-L277A 0.362210214662159 V164A-L262A 0.361679721964426 P63A-S129A 0.361212714486493 T106A-A127A 0.358475776557821 V64A-V128A 0.358154414649241 S101A-H226A 0.358132167938752 L98A-G267A 0.35378200160823 S93A-A275A 0.353148167958111 A136A-K158A 0.350639870505207 M75A-V255A 0.349679061460108 S101A-P278A 0.348952700592839 L98A-T137A 0.348673787848031 M96A-G279A 0.348007872141487 Q209A-L274A 0.346956330407947 Y235A-L251A 0.346779832970943 I229A-G256A 0.346322998749828 L70A-M75A 0.345675471428121 G238A-E254A 0.34361591419303 M75A-S101A 0.343085114805877 S104A-M257A 0.343008831999404 V99A-P278A 0.341632186043242 G95A-L277A 0.340950449368433 L82A-A222A 0.337742709053753 G261A-A280A 0.332962998377909 G105A-S260A 0.332506613903226 M96A-V205A 0.331850982677584 T78A-G100A 0.331658095709192 V99A-Q258A 0.331391875547707 I224A-F282A 0.33091684815142 I200A-G261A 0.3300950295548 A79A-G279A 0.329112423433802 Y235A-I253A 0.327706499878014 A67A-A127A 0.326797459756744 A65A-G237A 0.326523553249577 G95A-A222A 0.326018134862473 Q258A-H286A 0.325665436670949 K158A-I163A 0.324914315471092 A79A-G256A 0.324200800668859 G133A-A263A 0.32214260000232 A203A-G261A 0.321848933318952 L231A-A252A 0.319709302637252 S93A-K268A 0.317708292156268 G100A-A222A 0.316762780718689 S101A-A136A 0.316647484584719 S202A-P273A 0.31507398043858 P76A-G100A 0.313437910730766 A79A-G228A 0.313103314840572 G105A-G230A 0.313051871334745 H71A-G228A 0.310910288675174 Y72A-G105A 0.310095566893851 P76A-Q258A 0.309314043268811 S159A-A263A 0.308409124205447 S202A-A264A 0.306132187842393 V223A-L281A 0.304607575852187 M75A-G256A 0.304192900300083 Y72A-A103A 0.302804992022425 A65A-S126A 0.300451028493674 I201A-V284A 0.299123690354319 S208A-G267A 0.298531758607295 L98A-L266A 0.297720743918904 S104A-H286A 0.296511964919015 A222A-G279A 0.295906400574836 A79A-H226A 0.295421451332032 L92A-L277A 0.295299734202043 V205A-L281A 0.293270244133946 T106A-P167A 0.292340613405502 S101A-G256A 0.292012591240276 S208A-A276A 0.291756020083742 G230A-A252A 0.291384563504236 N227A-F282A 0.291075483933477 G230A-G290A 0.290376233377347 T68A-V128A 0.289132593578216 E254A-G290A 0.287865700579525 S93A-G279A 0.287670469596788 L98A-A264A 0.28736133114434 A83A-L92A 0.286388918571728 L232A-A252A 0.285975476637598 P138A-K158A 0.285866101105918 S159A-G267A 0.285441849024215 L92A-A276A 0.284356421275307 G100A-A264A 0.283804934200194 S202A-K268A 0.281228341961564 G95A-H226A 0.281189928185789 M75A-G230A 0.280495608831239 A67A-G234A 0.2788526544426 G105A-N287A 0.277860395350372 K239A-R249A 0.277238351748002 L131A-V165A 0.277131490333206 V102A-T137A 0.276278112544192 P76A-V99A 0.276242785944331 P61A-S122A 0.274722394318036 G216A-A280A 0.274179342972776 P138A-V154A 0.272944429175741 V219A-P278A 0.272916453796087 L231A-R249A 0.272853098052464 L70A-G237A 0.272032436208512 G234A-G242A 0.270981292380198 S101A-A264A 0.270244667798456 Y72A-G237A 0.266653161892192 G105A-Q258A 0.264307572396838 H226A-S260A 0.263861709088372 A83A-A222A 0.26288508597459 A66A-L251A 0.262778853435694 S202A-A276A 0.262023192617993 H71A-L232A 0.260896510868219 S283A-L288A 0.258824494716534 A62A-G237A 0.257509973539911 S101A-G267A 0.255726237214074 Y72A-H226A 0.255518074335194 L77A-G100A 0.255040388196609 T137A-T265A 0.25460807156179 S193A-G261A 0.254503963278894 H226A-S283A 0.254028283532003 I229A-C248A 0.252879635110506 G256A-W285A 0.252092004184813 S104A-G234A 0.252030008493289 L225A-H286A 0.251480312045583 A79A-L98A 0.251030359698247 A107A-S283A 0.250816984613652 C196A-V284A 0.250765526620347 S93A-P273A 0.250231058869483 L73A-S101A 0.247948623130699 G234A-Q258A 0.247270867457504 V134A-V164A 0.247140417223022 F282A-G290A 0.246998113880375 L98A-G133A 0.246303207114221 N227A-G279A 0.24579326769972 G238A-R249A 0.244224923307394 G105A-M257A 0.243464430041237 G230A-M257A 0.243440382481317 G216A-G279A 0.242282107173398 L70A-T78A 0.242056339129719 G207A-A264A 0.241106258763297 T130A-V165A 0.240660652569368 C196A-T265A 0.240516252078725 T141A-T265A 0.238002282663564 Y72A-A79A 0.237314101863703 S208A-A264A 0.237240427035719 T141A-L157A 0.236873860058906 A67A-R249A 0.236841140706307 R249A-M257A 0.236666739661872 T78A-L98A 0.236380631831158 L198A-S283A 0.236251829384041 M96A-N259A 0.235503161001364 Q162A-P167A 0.235370136357232 G256A-S283A 0.234934224402481 H226A-P278A 0.232185876479585 L98A-A263A 0.231676992129485 F69A-A103A 0.231161185683762 G279A-N287A 0.231151088521513 G100A-G228A 0.230769872816536 T265A-L274A 0.230186998371982 V64A-Y235A 0.229007837027861 G228A-F282A 0.22898193933907 P61A-L251A 0.228606259634842 A107A-G290A 0.22758357187301 V204A-Q209A 0.225511575265099 S93A-S208A 0.225226164126072 P167A-L262A 0.225192126915898 T130A-P138A 0.223757467628436 L82A-G95A 0.222508674136021 S193A-S202A 0.221532663975178 A103A-L131A 0.221430028529906 T124A-L251A 0.221395426738726 V134A-I166A 0.221166109133529 P76A-S104A 0.220553040837322 I197A-G279A 0.219838625554788 L73A-G234A 0.219611722052849 H71A-T250A 0.219111487159709 L82A-M96A 0.218187350468261 S93A-A264A 0.218031264464921 G100A-A136A 0.217807319836002 A103A-S122A 0.217232462110485 G95A-L274A 0.216671592508449 P76A-L225A 0.216251195072712 V219A-L277A 0.216208027956638 S260A-P273A 0.21549586324528 K268A-A275A 0.215491698769251 P63A-E254A 0.215399943873351 G261A-N287A 0.215345099480746 I74A-V97A 0.214694669559488 V204A-K268A 0.214441716967864 A107A-S129A 0.213819784377316 A107A-H286A 0.212957629079096 V223A-P278A 0.212706614547483 T130A-Q162A 0.2125052280472 F69A-V128A 0.212108616834509 V205A-S283A 0.211468985121087 S93A-V215A 0.211105247162443 V134A-G155A 0.210798497204365 V97A-T141A 0.210587991099035 V134A-L139A 0.209986890157772 W236A-T250A 0.209614795188641 N259A-G267A 0.208769665424151 V284A-S291A 0.208605951046472 N227A-Y235A 0.208384116210146 S101A-S283A 0.208312402313892 G95A-N259A 0.208137782446771 L82A-V97A 0.207811159877049 P273A-L281A 0.207525655765443 S129A-V165A 0.207027490281343 G256A-N287A 0.206671227660429 S104A-P138A 0.206281884331942 H71A-A136A 0.20604907578843 G105A-F282A 0.205254589284063 S93A-V218A 0.203965953070095 V123A-M257A 0.203620323626088 V102A-G256A 0.203498980327276 L77A-N227A 0.203454852159982 S126A-G256A 0.203014427360475 S101A-S159A 0.2010000116462 P76A-G234A 0.200979470729939 L131A-N259A 0.200841388664574 G238A-I253A 0.199543349805476 K268A-L277A 0.199223641990445 G256A-L293A 0.19861797517288 Q258A-P278A 0.198534353728941 G207A-L274A 0.198359403538617 L98A-G279A 0.197931796469717 V204A-P273A 0.19791889802307 G100A-G261A 0.197460514205789 L262A-G279A 0.197052401790711 F69A-G238A 0.196433363951231 S208A-L277A 0.1964233676675 T68A-P76A 0.195793701439128 P63A-H71A 0.195699579737859 V97A-L140A 0.194990787642096 S202A-G261A 0.194583645830446 V102A-L266A 0.194561718011553 T78A-I221A 0.194544915997872 L73A-T137A 0.194446254509941 A66A-S129A 0.193931986100187 G216A-P273A 0.193879932054075 I81A-T141A 0.193539770758706 Q258A-A263A 0.193316294407021 V204A-I269A 0.193105032675028 G238A-G256A 0.191939573006254 G207A-G279A 0.191649018249349 I220A-W285A 0.191069539124332 L70A-G234A 0.19069732952947 A264A-A275A 0.190631204728407 Y72A-S233A 0.190167080632446 S101A-G279A 0.189057170789873 P138A-T265A 0.18883805087682 S159A-A264A 0.188703399164155 G261A-P273A 0.188438890092713 G228A-C248A 0.187900403801923 P278A-H286A 0.187647533000209 G155A-I269A 0.18763720447753 P138A-S260A 0.187525516273689 T78A-G95A 0.186863984112179 W80A-G100A 0.186798193380193 M96A-S208A 0.186162394299239 P76A-N259A 0.185885801505469 G105A-N227A 0.185702032021655 N227A-S283A 0.185070126164899 L70A-K239A 0.184841101860642 P76A-S233A 0.184636126762927 N227A-L292A 0.184459443103289 V123A-I253A 0.184151964478178 G230A-G279A 0.18376350079809 T124A-I168A 0.183741786424134 N259A-S289A 0.183287897803484 G105A-P190A 0.18207246586484 Q258A-S289A 0.181855720669073 L231A-K239A 0.181227444601826 M75A-G237A 0.181188792718003 H226A-G234A 0.180868818497657 A65A-T250A 0.180660508621645 M96A-G216A 0.180546364573067 L232A-T250A 0.180321792812707 G133A-S159A 0.179750679896618 G105A-L231A 0.179137550026798 A62A-T250A 0.17900293397044 T68A-G100A 0.178804076686511 G155A-T265A 0.178719379658681 I74A-T141A 0.178645073449232 G228A-N287A 0.177964168816792 G267A-L281A 0.177677698161076 H71A-G105A 0.177501882762786 L98A-S283A 0.177272590778848 S260A-S289A 0.177147729435786 P138A-L161A 0.176866769723155 A66A-G237A 0.17551647160004 Y72A-L140A 0.175057447996175 T106A-G133A 0.174462996986623 I253A-G290A 0.173383496247266 G95A-I269A 0.172811246603905 A83A-I221A 0.172706732513968 S101A-L157A 0.172273333831318 I201A-G261A 0.172246058595498 Y72A-L77A 0.171627373566897 G230A-I253A 0.170290311328515 P63A-T68A 0.170098034588563 I74A-G234A 0.169625301703029 L262A-P278A 0.169401806937915 M96A-P278A 0.169376300168759 A79A-T137A 0.169172961939923 G267A-L274A 0.168423903949791 L77A-G256A 0.167991750329215 A276A-V284A 0.167441489835573 M75A-G95A 0.167070854573372 V223A-A264A 0.166982574084561 A107A-V255A 0.166465684043616 L139A-G155A 0.165688124705614 A66A-A252A 0.165508593004617 G207A-L281A 0.165081749225483 M75A-G279A 0.164643047376272 G95A-T137A 0.164244054670355 T106A-H286A 0.164209502020569 M75A-G100A 0.164161416368391 V215A-A276A 0.16392394349667 G228A-Q258A 0.163593713162793 S104A-V255A 0.16328476405191 S202A-G279A 0.163058811648043 G100A-G133A 0.162299253667562 Q258A-T265A 0.161777939312619 G207A-A263A 0.161636831452177 G105A-A136A 0.161636831452177 P63A-T250A 0.161196120703721 T137A-A264A 0.16103150221728 I220A-H286A 0.161003840718304 A83A-G267A 0.160834570995975 G133A-L266A 0.160658179482454 V195A-V284A 0.160308253628293 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