[Wed Aug 24 17:16:41 2016] >Input file: TM4NPQCGD.pdb >TM segments: A192-214_A216-239_A249-272_C812-836_C838-861_C871-894_B502-525_B527-550_B560-584_B597-620_E1435-1458_E1460-1483_E1492-1515_D1123-1147_D1149-1172_D1181-1204 MAIDEN score = -295.1747 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- I1127D-L1132D 1.19624233140072 I505B-L510B 1.19304083284189 I816C-L821C 1.18807957831696 I194A-L199A 1.183450634906 I1438E-L1443E 1.1783092266305 I547B-L237A 0.96997172707356 I1480E-L1170D 0.93060502934188 I1169D-L859C 0.84375505674488 L548B-I858C 0.842889874450317 E579B-I598B 0.829452048871916 I829C-L845C 0.811621401181685 L1481E-I236A 0.81061590930522 I207A-L223A 0.809521782006641 I1140D-L1156D 0.807977314407883 I518B-L534B 0.807535053473302 I1451E-L1467E 0.807115206937228 Y839C-V891C 0.753928764298434 S814C-I819C 0.750963443557862 S192A-I197A 0.748030543995439 Y1461E-V1513E 0.747067907605002 S503B-I508B 0.74549158862281 Y1150D-V1202D 0.744966071739523 Y528B-V580B 0.736566343142087 Y217A-V269A 0.735488687446028 S1436E-I1441E 0.734054438993158 S1125D-I1130D 0.732517604582338 T222A-T227A 0.711546813503301 V846C-Y884C 0.710758165730741 V224A-Y262A 0.710752239519922 N1464E-V1513E 0.709680489902389 N1153D-V1202D 0.709460126051555 T1466E-T1471E 0.709419200260591 T844C-T849C 0.709111819560101 N531B-V580B 0.707745464550973 N842C-V891C 0.707194287500635 T533B-T538B 0.704976632949116 N220A-V269A 0.70146151683115 T1155D-T1160D 0.699118481576335 T533B-T222A 0.685891600981311 V535B-Y573B 0.682664079279633 V1157D-Y1195D 0.682418702979611 Y561B-L617B 0.682215086271013 L539B-A230A 0.676092245689259 L1472E-A1163D 0.67540027867322 A541B-L850C 0.672463135702463 V1468E-Y1506E 0.670563856921243 L1161D-A852C 0.669050504025537 I543B-F234A 0.668188003438635 I1476E-F1167D 0.667240226719509 F545B-I854C 0.666461272735112 A1474E-L228A 0.665686842690793 I1165D-F856C 0.660338469495679 F1478E-I232A 0.64895110780445 V608B-A613B 0.64801793179529 T1466E-T1155D 0.635301903999666 Y1150D-A841C 0.629815113990999 T533B-T844C 0.622926859614559 V532B-T222A 0.613788534681129 F1447E-F263A 0.603378647947284 T581B-T210A 0.600383337799608 F514B-F885C 0.59901455000074 F1196D-F825C 0.598763065797322 V1465E-T1155D 0.590056308027242 Y1126D-I1131D 0.58353546406905 A1463E-Y217A 0.581680994671049 T1514E-T1143D 0.570513599713424 Y504B-I509B 0.568888412884796 F1507E-F1136D 0.568769791612334 F1189D-P817C 0.567635153216615 Y815C-I820C 0.567163990670796 F574B-F203A 0.564561559602326 Y193A-I198A 0.563804123089951 T533B-V843C 0.560135394885397 P506B-F878C 0.559055809058552 Y1437E-I1442E 0.55437782666717 T1203D-T832C 0.553888762689532 V1154D-T844C 0.55387696391036 P1439E-F256A 0.550260949936579 T1466E-V221A 0.546611993775682 Y1461E-A1152D 0.544033771660642 F567B-P195A 0.541613279859086 Y528B-A219A 0.541095538056856 A530B-Y839C 0.539114080165918 F1500E-P1128D 0.538077049288457 N546B-L237A 0.530797776268697 N1479E-L1170D 0.528325208226768 T1155D-T844C 0.526690600530891 S537B-S848C 0.52618913554659 S1159D-S848C 0.520814494797576 V532B-A219A 0.518754820423262 S1470E-S226A 0.517526533616222 S537B-S226A 0.516004741357859 S1470E-S1159D 0.515798738582992 N1168D-L859C 0.510242633279779 T1466E-T222A 0.506843809964188 L548B-N857C 0.505058930155305 A530B-V843C 0.504958598183116 V1465E-A1152D 0.504573631109979 A1463E-V221A 0.494980776533017 V1154D-A841C 0.493638542843097 T1454E-T270A 0.490875814831218 T521B-T892C 0.473315195627452 V1154D-S1159D 0.467261885215677 V532B-S537B 0.464830907565948 V843C-S848C 0.463080862999089 V1465E-S1470E 0.462937387643881 V221A-S226A 0.462738065977504 L1472E-Y1506E 0.45577634470411 L539B-Y573B 0.4554869253327 L1481E-N235A 0.450592018554003 W1453E-A1509E 0.443183051303006 L1161D-Y1195D 0.439342469137904 L850C-Y884C 0.438845300394652 F523B-R603B 0.438688240268656 I1162D-F1167D 0.43639987146038 I1473E-F1478E 0.434818591571374 Y1192D-F825C 0.434710240548212 I851C-F856C 0.434070736924597 W1142D-A1198D 0.433708153619024 L228A-Y262A 0.433513131914534 I540B-F545B 0.432904891203471 I229A-F234A 0.431016171512931 W520B-A576B 0.430925369510346 W831C-A887C 0.426874888067395 M1492E-S1125D 0.426528605453561 W209A-A265A 0.426223884839704 F523B-R600B 0.422510731144075 H542B-M568B 0.419166135274313 F523B-S602B 0.411277845943025 H1164D-M1190D 0.408852154298788 H1475E-M1501E 0.408541127891715 V1158D-L845C 0.407336755912973 L1467E-V225A 0.407121637049438 N220A-V225A 0.405477219754821 H231A-M257A 0.400638541163056 N842C-V847C 0.399055100562969 N531B-H584B 0.397935492726241 H853C-M879C 0.397140031103963 I543B-A233A 0.395579170727103 N1464E-V1469E 0.395320399062484 N531B-V536B 0.39492363677305 N1153D-V1158D 0.394832992370693 I1476E-A1166D 0.393757183842553 V1469E-L1156D 0.393454702189931 I1137D-A1163D 0.393409190221023 I204A-A230A 0.392108113382964 A211A-V269A 0.390183363635855 A833C-V891C 0.390124465587714 A1144D-V1202D 0.389602779006359 T1454E-E1512E 0.389570852166211 A522B-V580B 0.389147112148409 V1468E-A1509E 0.389080282091861 A1455E-V1513E 0.388993571218556 T521B-E579B 0.388810533938513 V577B-V582B 0.388241605009433 V535B-A576B 0.388217037407618 V224A-A265A 0.387494288487376 L534B-V847C 0.387443115094935 V1157D-A1198D 0.38718429807657 V846C-A887C 0.386968280918204 T832C-E890C 0.386602203733268 T1143D-E1201D 0.386208079469068 I515B-A541B 0.38570771388542 V1199D-V1204D 0.385566044802145 I540B-A230A 0.38524786271415 I1473E-A1163D 0.383295054256657 T210A-E268A 0.381644785131787 A541B-I851C 0.379388200560287 V1510E-V1515E 0.375837222678043 H1475E-H231A 0.375453837408954 I826C-A852C 0.375219779452248 A211A-L223A 0.373667637729119 V888C-V893C 0.372752126810067 F1507E-I1140D 0.372469524715959 A1144D-L1156D 0.372237752832548 V266A-V271A 0.370566628244124 A522B-L534B 0.369229775460806 Y839C-T844C 0.368823892394934 A1474E-I229A 0.368293811745433 Y528B-T533B 0.368040598931779 I1162D-A852C 0.367147423179364 Y1461E-T1466E 0.366657315276253 I540B-I229A 0.366334790166882 A833C-L845C 0.366086406504076 Y1150D-T1155D 0.365715563750584 Y217A-T222A 0.364744323336314 A1455E-L1467E 0.364720381065252 Y570B-F203A 0.364516411613591 S1141D-T1160D 0.363903886863959 F514B-Y881C 0.363850572249737 V536B-A541B 0.362497001987855 S208A-T227A 0.362028196871196 M1445E-W1453E 0.360878672109323 V536B-L223A 0.359958791188033 M201A-W209A 0.359739213902497 S519B-T538B 0.359219798538248 V1469E-T1160D 0.359151610527234 I1448E-A1474E 0.357992550175548 I518B-F885C 0.357878609802082 H1164D-H853C 0.357001103497427 S830C-T849C 0.356589677965981 Y573B-V609B 0.356306124676001 S1452E-T1471E 0.355916322898409 A544B-I854C 0.355667332644538 I1473E-I1162D 0.355605572937784 M512B-W520B 0.354440825551226 M1134D-W1142D 0.353164554444023 V847C-A852C 0.352617670710941 I1140D-T1160D 0.352596340036002 H542B-H853C 0.352021953501573 T521B-E529B 0.351274920696222 S1452E-Y1506E 0.350500222873039 M1445E-I1502E 0.348388115025902 I204A-T227A 0.348132671707296 M823C-W831C 0.348026486085736 T210A-E218A 0.347447058166078 I207A-T227A 0.345906659986964 T1143D-E1151D 0.345885205203324 T832C-E840C 0.345615458369988 H1475E-H1164D 0.345209495812849 I829C-T849C 0.344483893670264 V536B-T227A 0.344328273660727 M512B-I569B 0.34423521106315 I826C-T849C 0.343032576496618 T1143D-L1156D 0.342893193572603 T521B-L534B 0.342754741407333 A522B-A601B 0.342570156446544 A522B-S527B 0.34139008699328 A1455E-S1460E 0.34135493674623 A833C-S838C 0.341276032846858 T1454E-E1462E 0.341203748114095 T832C-L845C 0.341078733052816 A1144D-S1149D 0.34103088693026 T210A-L223A 0.341022117949512 A211A-S216A 0.340670475660981 E1172D-F856C 0.340578801470808 S830C-Y884C 0.339702311962494 S1141D-Y1195D 0.339632535908064 T538B-V847C 0.33943587831437 S519B-Y573B 0.339147638871816 I515B-T538B 0.337672050242951 I1165D-A855C 0.33740589144505 E529B-Q583B 0.337197600054544 V225A-A230A 0.337166780252257 E218A-Q272A 0.337118721313788 I1137D-T1160D 0.337039644913557 V224A-V266A 0.336948341837085 V577B-M201A 0.336433635386711 E840C-Q894C 0.336103197684403 V1469E-A1474E 0.335215300513649 F1447E-Y259A 0.335067536979478 I518B-T538B 0.335029274624825 I1451E-F263A 0.334637746628613 V846C-V888C 0.333740740620545 V1510E-M1134D 0.333414161432537 F574B-I207A 0.332961027081118 I540B-I851C 0.331526612188191 V1157D-V1199D 0.331078644303639 V1199D-M823C 0.330959727967842 F1196D-I829C 0.33057828482204 T222A-T270A 0.330431938569429 M823C-I880C 0.329749957090076 S208A-Y262A 0.328747720956565 M1134D-I1191D 0.328665345775766 E1483E-F1167D 0.328509638862344 I1451E-T1471E 0.326560133903363 F545B-E861C 0.326114907746099 M1181D-F813C 0.326092978159837 F610B-I615B 0.324585572024809 Y570B-V609B 0.32382964417451 T1454E-L1467E 0.323780596226159 L1138D-T1160D 0.322678758294337 T844C-T892C 0.322358102994812 T1155D-T1203D 0.322233422218861 M1445E-V266A 0.321268121673995 S1452E-A1509E 0.321103509593521 M201A-I258A 0.320907896534754 I1448E-T1471E 0.320897994600692 S597B-A605B 0.320331525266347 T533B-T581B 0.320033248442066 I194A-M201A 0.320020875289153 S830C-A887C 0.320001641362013 L205A-T227A 0.3195926545778 I518B-V888C 0.31948945459638 T1466E-T1514E 0.319207871133262 S519B-A576B 0.319122962679202 S1141D-A1198D 0.319058982658912 S208A-A265A 0.318308427945182 L516B-T538B 0.31755169636807 V535B-V577B 0.316255487662425 L827C-T849C 0.316140015374812 M512B-V888C 0.315749909662645 F1478E-E239A 0.314335572607865 A1163D-S848C 0.313624045749327 I1451E-V266A 0.313483197685684 I1438E-M1445E 0.313332580617788 L1449E-T1471E 0.313174443183844 V1468E-V1510E 0.312771019363569 S1470E-A230A 0.311838585312756 I1162D-I851C 0.311040086616873 H542B-H231A 0.309726926291969 S1125D-P1133D 0.309717894277896 A1474E-S1159D 0.308795193376331 E550B-L237A 0.30729529124852 S537B-A852C 0.307131772005458 M1181D-A1186D 0.306714327838923 S1452E-A1474E 0.305610084634421 V1158D-T849C 0.304902101416404 V1158D-A1163D 0.3048620735638 S830C-A852C 0.304657688778241 I1473E-I229A 0.302101549368886 V609B-N614B 0.300500623815577 L1193D-A1198D 0.300303978135611 F212A-E218A 0.300061497676854 L882C-A887C 0.299863977082098 L571B-A576B 0.299232995459199 I1140D-V1157D 0.299189915186771 L1504E-A1509E 0.299007636639201 I1451E-V1468E 0.298731391191154 S519B-A541B 0.29840598398761 Y573B-P607B 0.298303290439825 I518B-V535B 0.298296193037844 L260A-A265A 0.298279762231844 I207A-V224A 0.297333963290534 T521B-P607B 0.29627645722446 S208A-A230A 0.295539483062685 T599B-P607B 0.295261360198501 I829C-V846C 0.294463851912075 F1500E-N1129D 0.294296416931428 S1141D-A1163D 0.294193818662088 I547B-I236A 0.292328654558107 V1199D-I829C 0.292297011443937 N1440E-F256A 0.292009164376652 V1510E-I1140D 0.291789642723872 V577B-I207A 0.291729264610006 N507B-F878C 0.291681225291778 T1471E-V225A 0.291037708155058 I505B-M512B 0.290677526500097 L1161D-A1166D 0.289493202160318 A1477E-I232A 0.288737454707224 L850C-A855C 0.288668403248008 L539B-A544B 0.287014759256174 F523B-E529B 0.287008622174009 A564B-L617B 0.286709885372958 F1189D-N818C 0.286671946177304 L228A-A233A 0.286353959842644 L1472E-A1477E 0.286037091699477 M1492E-A1497E 0.285945906305898 S1436E-P1444E 0.284553943257558 F1145D-E1151D 0.284181648832117 F567B-N196A 0.283942126807466 A541B-S226A 0.281532334511671 S503B-P511B 0.280824890153069 M1181D-S814C 0.280423926045214 F834C-E840C 0.280000590194759 S208A-H231A 0.278453090130188 E1483E-T1495E 0.276746007765066 I816C-M823C 0.274646323058731 E550B-T562B 0.274280671539136 S1141D-H1164D 0.272490043989503 S1452E-H1475E 0.272233831694725 F1456E-E1462E 0.271932470572392 S519B-H542B 0.27148601737274 N531B-E1151D 0.268271459158876 E529B-N1464E 0.267612405223759 N1153D-E218A 0.267612405223759 S830C-H853C 0.267517072286601 I565B-N614B 0.266521113953605 S192A-P200A 0.266358959749932 A576B-A605B 0.266305273000163 A1455E-A1463E 0.266129542987966 E550B-F234A 0.264991443271715 S1470E-H1164D 0.264267854530963 I1127D-M1134D 0.263744026629038 H584B-S597B 0.263316621327633 P607B-L612B 0.26238961524342 L1467E-V221A 0.262361282538696 S519B-P607B 0.262062775419388 V1154D-L845C 0.262004033227216 A833C-A841C 0.261331453643053 E239A-T251A 0.260892646674682 A211A-A219A 0.259534191041541 A1144D-A1152D 0.259352575512362 S814C-P822C 0.259309138736848 A522B-A530B 0.25908609894529 F502B-G874C 0.258312304620345 E1172D-T1184D 0.256597418140628 Q583B-S602B 0.256501911233232 A576B-R603B 0.255332162350302 V1465E-L1156D 0.25464727664287 L534B-V843C 0.254520292961641 V532B-L223A 0.254214571811103 N614B-F619B 0.253478386935264 Y1192D-H853C 0.252532366534446 N1168D-G1185D 0.252449513131538 I1480E-I1169D 0.252306862118873 N546B-G563B 0.251766030541848 I1476E-L1170D 0.250736956756629 H1475E-Y259A 0.250353521343698 H542B-S848C 0.250270614029553 Y570B-H231A 0.249563540733184 N1479E-G1496E 0.248314520300629 T533B-T892C 0.246481535235919 L1161D-P822C 0.246056374121288 T1466E-T270A 0.24556432105714 L1472E-P1133D 0.244846471383313 S537B-H231A 0.244569409015581 T1514E-T1155D 0.244438445913945 E861C-T873C 0.244321290928183 Y1503E-H1164D 0.242812016101656 L539B-P200A 0.242536930151034 T1203D-T844C 0.240682495619965 N857C-G874C 0.24011202398537 G563B-N614B 0.238134415146612 T581B-T222A 0.23770125118402 N235A-G252A 0.236263456215598 P511B-L850C 0.235466729198939 A576B-F203A 0.234454535124062 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