[Wed Aug 24 17:26:16 2016] >Input file: TM4O9PATD.pdb >TM segments: C3-20_C37-53_C61-81_D3-19_D32-49_D55-72_D86-104_D113-134_D153-172_D178-242 MAIDEN score = -103.5498 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- V127D-G191D 3.77410777463558 N75C-Y88D 1.47281945816402 H42C-N75C 1.3592029359642 I70C-L62D 1.22341499528702 I87D-L133D 1.21681224557314 F15D-W34D 1.14396970730159 I99D-E221D 1.13583718877007 I167D-F183D 1.10144928976578 I4D-W49D 1.08877036080461 V127D-F190D 1.00465042473482 Y20C-T242D 0.991330856447357 I70C-A61D 0.982337775800726 V52C-I99D 0.967819708445694 I134D-V192D 0.955864210936107 F12C-V232D 0.950654445076957 L53C-E61C 0.936358944520657 Y80C-I32D 0.894038685686619 A74C-W70D 0.886571005246635 I70C-L64D 0.855661465959425 L14D-W34D 0.792165092712336 G18D-W34D 0.77686994385141 V45C-V218D 0.773363562821453 G78C-W70D 0.768473665832091 V160D-L186D 0.731830218618471 V47C-V43D 0.731019273992398 V160D-F190D 0.726689229398922 E221D-L230D 0.685903044366279 V69C-G65D 0.683470468788081 G18D-V33D 0.679858243574716 G48C-I99D 0.677711842570484 F66C-I58D 0.672974791225972 V218D-L230D 0.672636202971871 Y20C-L236D 0.671690581465341 I41C-F15D 0.669480603868228 A128D-F213D 0.669096227533983 A86D-L133D 0.668697796810454 A118D-F220D 0.666218537743481 L161D-F190D 0.66313465153862 L53C-I58D 0.661455735174211 V10D-G38D 0.657434103351464 Q5D-P228D 0.656170765191157 V52C-E103D 0.651849921989805 V46C-G72C 0.648820585867778 N89D-A128D 0.646177619695974 A128D-N211D 0.646167658184194 A116D-L172D 0.643666204729589 N75C-A69D 0.640474903127146 V17D-A35D 0.633130417446458 V47C-A229D 0.629823241268512 V218D-A233D 0.6288582375404 I41C-A76C 0.627455175276743 G92D-F129D 0.625496552970397 G93D-F129D 0.618007086147019 G125D-F213D 0.617571445485631 F209D-G241D 0.617483924329689 I41C-A35D 0.614980739490551 F3C-G226D 0.613089116416098 S126D-G191D 0.610145458598121 F220D-G234D 0.604895913927082 F129D-G191D 0.603866101845511 F40C-A77C 0.60350371532678 H42C-T214D 0.595707356651125 S126D-L188D 0.594283539525268 F40C-G78C 0.592993295716482 L18C-L236D 0.591920321997087 A7D-L41D 0.587683726798991 E103D-A118D 0.585800869824462 V47C-T46D 0.584792818330438 V51C-W49D 0.579384798952292 V69C-I58D 0.576083133227037 V17D-W34D 0.572022564533901 N39C-G79C 0.570470716536506 I64C-V102D 0.570236767783965 Y80C-G36D 0.5685934220952 F40C-Y80C 0.568405827962143 N157D-F190D 0.561958451432786 L171D-S179D 0.557203344178261 G93D-V127D 0.556104483816402 V44C-L230D 0.554821871821666 F209D-T242D 0.553011124559412 I70C-S66D 0.552970553646112 A81C-W70D 0.54365153528411 Y20C-A240D 0.542933233643611 N89D-I207D 0.542088308404137 V127D-L188D 0.540552208469848 A118D-V225D 0.53546363366259 N39C-V237D 0.534050734770264 V69C-L62D 0.533587098196328 F15D-A233D 0.531986309400245 G37C-I32D 0.528543461680341 F129D-L188D 0.527654609334704 S208D-G241D 0.52506233124077 V47C-A42D 0.524409265270606 S38C-L19D 0.524038463572086 N75C-I87D 0.523290419754816 F9D-A229D 0.522037547600172 A76C-Y88D 0.520929754729171 V46C-A95D 0.512777586855813 F17C-A239D 0.51221664043078 V44C-G72C 0.512003719650414 F66C-A61D 0.508956775721446 G131D-Y210D 0.507191603643044 L114D-F223D 0.506249971239115 A96D-T214D 0.505703241972128 F223D-L230D 0.504548557462713 F3C-A8C 0.504325801358143 A97D-L123D 0.503159900808145 L53C-F66C 0.503107459124387 L117D-F223D 0.501935632288812 S126D-F184D 0.497005151903052 V11D-W37D 0.496339113697734 L123D-F184D 0.496322281473121 V11D-A229D 0.495928749784501 V46C-G68C 0.492558412930199 L117D-F220D 0.490036936406021 A8C-F223D 0.489527376079062 A96D-E221D 0.484508745662235 N211D-G241D 0.483761671423338 L171D-I180D 0.478227565697553 L161D-A187D 0.477665836143027 F12C-T235D 0.476332217477946 I119D-G168D 0.474101681019446 G78C-S66D 0.46828593677259 S170D-S179D 0.46815908078918 V46C-A76C 0.466489679309809 V44C-A229D 0.465242636129365 H42C-A76C 0.464977548218374 V46C-V69C 0.462084804341548 V45C-L230D 0.461144104649401 A74C-V67D 0.460732037081869 I167D-L182D 0.459062529734865 A69D-Y88D 0.458837297478273 V11D-L41D 0.457209631959872 I41C-V237D 0.456885584951369 L114D-A224D 0.455736885223509 G65C-V102D 0.453969168442353 I41C-G79C 0.45065437412524 G19C-T235D 0.444455494437233 L71C-Y88D 0.441684216899782 A100D-E221D 0.438255768031178 N89D-Y210D 0.435035538664647 L19D-A35D 0.431825622865947 F66C-L57D 0.428720298393603 L120D-F220D 0.423368563333789 A49C-T98D 0.422508220006989 Y8D-L41D 0.42114101058507 V44C-A42D 0.420936060665474 L67C-A61D 0.41920324124123 Y8D-A45D 0.411728522130671 I134D-L196D 0.405988737717371 G68C-I99D 0.405137393138446 A77C-V67D 0.400976055853306 I87D-S132D 0.398500885812324 A12D-A233D 0.397124636992735 N39C-Y88D 0.396681341176909 L53C-V69C 0.394309859275718 A100D-I119D 0.392948562994267 G222D-P228D 0.392070446016763 A74C-V68D 0.388167520770851 V44C-A233D 0.387415990057886 I124D-T214D 0.381277813612327 L123D-F183D 0.381274064810215 A212D-A239D 0.377638505177387 G37C-Y80C 0.375944592688385 A76C-A42D 0.375528130523965 G65C-I99D 0.374359180569559 V127D-L189D 0.37059013891253 V160D-L189D 0.369242967106788 H42C-N89D 0.369072081456636 A116D-L169D 0.368776323675293 G121D-F220D 0.367059074792564 Q153D-P197D 0.36497400085233 L67C-T98D 0.362547019379473 I99D-G122D 0.362364444069646 I124D-F213D 0.362294837288201 G48C-L230D 0.361223731127787 L120D-A217D 0.359761408461655 V46C-V43D 0.358335898886333 A42D-A233D 0.357814549887576 A86D-I207D 0.356639437603294 L19D-I32D 0.355225184900725 V218D-G234D 0.354020795935831 I4D-F48D 0.351360716051864 I70C-G65D 0.350539846692558 N211D-V237D 0.349794497684336 T130D-L189D 0.347765509625598 N89D-P204D 0.345698021575153 A76C-S66D 0.341495688732675 H42C-G92D 0.341039792334894 F12C-E221D 0.341031109342263 T98D-G122D 0.340093204762682 A96D-A224D 0.339113674494982 A96D-G121D 0.337621789556735 L123D-T164D 0.337390193550293 G215D-V237D 0.33723147453338 V160D-A187D 0.334184320723146 V47C-L230D 0.333687534002015 Y8D-P228D 0.333110049624489 V47C-G72C 0.332290435552236 A97D-G121D 0.330963287780718 S208D-T242D 0.330780685466632 T214D-G238D 0.329904027090156 G90D-A217D 0.329874176398982 A49C-A95D 0.327080059790871 G37C-A77C 0.32540387863284 A233D-G241D 0.324349945560982 I4D-A45D 0.324101631077595 G37C-L19D 0.322973199676721 F213D-E221D 0.322811085411662 G93D-V218D 0.322720672080102 G93D-I124D 0.319759555858205 G191D-A206D 0.318807027055196 V52C-V102D 0.318417231936345 G93D-E221D 0.318303478829531 T98D-T214D 0.316384295275435 S38C-G241D 0.314630838578458 G92D-G238D 0.314560963632301 G37C-G238D 0.314448247980584 G68C-G122D 0.312289834754873 S170D-A178D 0.311264757320111 Y80C-G92D 0.310683060718299 F223D-P228D 0.310609949109363 H42C-G18D 0.309474734837115 G19C-H42C 0.309205802574072 A95D-G215D 0.308515649252997 I11C-V232D 0.307254138000235 L230D-T235D 0.307107074163886 G19C-G219D 0.306954125180199 V46C-L71C 0.305833589826297 G43C-A73C 0.302825295962011 I124D-A187D 0.301705697824413 A100D-G121D 0.301669439689098 A212D-A233D 0.300862496087568 G93D-A217D 0.300370770734208 G37C-S208D 0.300092844564987 L3D-A45D 0.300017013628606 S38C-G92D 0.29970185402199 I64C-A101D 0.298754253024075 G72C-G215D 0.298667453830868 G48C-A217D 0.297840076813316 A97D-I124D 0.297611820156489 V218D-T235D 0.294979245306352 L163D-L186D 0.294648355688184 G94D-A187D 0.294555371309855 Y80C-G65D 0.293653724500059 A158D-G191D 0.293595346191336 L123D-A217D 0.293440860186735 L67C-A95D 0.293333546156699 G37C-G38D 0.293296332881082 L123D-V165D 0.2928068680442 G113D-G226D 0.290761719865129 G43C-G125D 0.289533709887968 Y210D-G234D 0.289434024659093 H42C-A233D 0.288906780735797 G122D-L186D 0.288506817862932 G4C-G113D 0.288119179878694 G18D-A233D 0.287682086241114 L18C-A239D 0.287437006316922 G122D-L185D 0.287404576619179 L104D-G168D 0.286958457267411 L14D-W37D 0.286304860432729 A101D-S179D 0.285853606292919 A7D-A42D 0.284998445497695 G121D-L230D 0.284145431219522 G92D-L133D 0.284037131135662 G79C-N211D 0.283175297155573 V46C-Y80C 0.283118835131267 T195D-P204D 0.281688881604018 V218D-V237D 0.280791339157492 A100D-G168D 0.280568946789174 L104D-A118D 0.279449152838175 G168D-L185D 0.279203289858859 A73C-L63D 0.278572830576151 V218D-V232D 0.278500909118771 G121D-Y210D 0.278328026308751 V52C-V69C 0.277993166197455 V45C-Y8D 0.277638418117103 V46C-N75C 0.276414547252523 G43C-G238D 0.276408935940572 G48C-G93D 0.276148064971594 G219D-A233D 0.275677988685768 S208D-A239D 0.275065759173581 P197D-P204D 0.273562353488319 F220D-L230D 0.271647460068477 G122D-F184D 0.271427985664438 L19D-G241D 0.271393404759607 Y20C-G234D 0.270524831522348 L120D-G222D 0.268039253785455 L71C-G92D 0.267814830773126 G68C-L64D 0.267620587547512 G48C-E103D 0.267236146833874 T214D-G234D 0.267223215830086 G48C-L71C 0.267092890135223 L230D-G238D 0.266274337421733 Y10C-P228D 0.265984080303619 N89D-F129D 0.265182620194459 N39C-G72C 0.264975894534756 G43C-A217D 0.261940101969482 G65D-A95D 0.261482639464018 E103D-V225D 0.260526240122837 A76C-G90D 0.258084346570261 G48C-G121D 0.257909124744112 G125D-F190D 0.256552790150098 G113D-G121D 0.25601339771397 A73C-G94D 0.256006987967504 G191D-F213D 0.255617936849395 G90D-A212D 0.255438707835148 L114D-V225D 0.254505378362992 L71C-G125D 0.25337843848864 Y20C-V232D 0.252621596039395 I16D-A233D 0.25174756036263 A212D-T242D 0.250258442689265 A16C-A240D 0.249882657156142 I70C-A95D 0.248812084849938 Y88D-G215D 0.248180484079661 H42C-L230D 0.247693718659688 V69C-A95D 0.247647123619421 A42D-A229D 0.247511485441967 A74C-G93D 0.247475998988457 T15C-V232D 0.246786895017856 A12D-V232D 0.246473683084751 A128D-L133D 0.244933955747052 A217D-A239D 0.243693095248204 L71C-G79C 0.242900526095051 G131D-S208D 0.24266923439779 L120D-T214D 0.242174734659119 L9C-T235D 0.242117578673134 L123D-T130D 0.241724120442521 A49C-G93D 0.241311822239244 L19D-G38D 0.240621256215361 G48C-F220D 0.240604457802359 A217D-G234D 0.240428829245496 T214D-L230D 0.240390322664431 A101D-G168D 0.239905073938773 E103D-G121D 0.23966570088693 N75C-G215D 0.239154699343265 T214D-L236D 0.238015877071191 G226D-G234D 0.237500846746008 I70C-N75C 0.237459157184553 A16C-A212D 0.237098570837373 Q5D-N227D 0.236665755154881 S38C-A240D 0.235597759765792 G90D-L133D 0.234437647105187 G215D-L236D 0.234425913350905 L18C-A240D 0.233712815066008 G48C-N227D 0.233666028327708 L188D-T195D 0.233216172026678 A12D-A229D 0.232878366399657 G219D-G241D 0.232315652883216 N75C-G125D 0.232066153223645 V67D-A72D 0.231850438423113 N211D-G238D 0.231207110577657 G79C-W71D 0.231201650402378 N89D-G234D 0.231172388339427 A96D-S126D 0.231097472226695 V45C-E221D 0.230850094149904 G48C-N75C 0.230511934938611 L71C-A95D 0.230409299534079 G93D-A187D 0.230140079010051 G90D-S208D 0.228779587543511 H42C-G121D 0.228659915124557 G65C-L62D 0.227345315085815 G65D-G94D 0.22668765566778 F213D-G238D 0.225484190741537 G90D-L188D 0.225240509410141 I119D-V166D 0.224956485699639 A49C-A96D 0.224280373366122 N75C-G36D 0.224208969509611 K154D-L159D 0.223901503166381 A73C-A61D 0.223705682054478 T130D-L186D 0.223479919520221 A7D-F47D 0.223199518468317 V51C-T46D 0.222987119262908 V127D-V192D 0.222352492797292 V127D-Y210D 0.221661461736817 G122D-A217D 0.221414081793997 I99D-A118D 0.221363032420794 T164D-L171D 0.220504614747608 I41C-V40D 0.220168805633432 Y20C-A212D 0.220106449259214 A118D-S126D 0.218898299067773 T98D-T130D 0.2186761700491 G125D-V218D 0.218508441623104 A49C-G72C 0.217860698756263 A49C-I99D 0.216002617916843 S126D-L186D 0.215932514994662 G121D-A187D 0.21585971728809 V43D-I58D 0.21563305165947 G72C-L63D 0.215212553078056 G4C-N227D 0.214918542819935 N157D-L189D 0.214728699719225 A49C-G65C 0.214626481707038 V45C-G92D 0.214507713093208 F40C-A86D 0.214321182704084 G168D-A178D 0.213488567213533 L161D-L186D 0.21312802077722 H42C-A77C 0.212545687873417 G48C-V102D 0.212447068601385 Y80C-V68D 0.212155940100182 N89D-G241D 0.212136261608742 G90D-T98D 0.211247398894476 I41C-G241D 0.210889468448427 T15C-G241D 0.210341939521543 G122D-T130D 0.210256925187041 V52C-E61C 0.210056870936013 F209D-V237D 0.209971198020522 G43C-V40D 0.209881587084114 V47C-L44D 0.209054814541364 A206D-G241D 0.20879417822786 G113D-G222D 0.208636383676907 V69C-L59D 0.207657919973647 F40C-V46C 0.207446365957024 V44C-G93D 0.206793570768303 N39C-G18D 0.20626527171056 V127D-L193D 0.205910638434804 F220D-A229D 0.205630411579035 G90D-T195D 0.205328751125935 P204D-T242D 0.205055391882823 V127D-F209D 0.204682407372788 G18D-I32D 0.204483843994533 N39C-A76C 0.204281902996517 G92D-A212D 0.203655842100944 N39C-G36D 0.203271457417153 V44C-V218D 0.203103188061879 V127D-L186D 0.202453525234427 Y20C-G215D 0.202273838972607 I41C-V33D 0.202139049218034 G222D-G238D 0.201830128361497 G48C-A233D 0.2017029672819 G113D-L169D 0.201326163435471 G92D-I99D 0.201283056257996 G215D-P228D 0.200526258564117 G122D-G215D 0.200463475637938 V69C-L60D 0.200252822135335 V69C-Y88D 0.200189794460549 G43C-T235D 0.199592292389137 N211D-G219D 0.199357057914596 V11D-G38D 0.19929855640537 V69C-L64D 0.198964914840375 G93D-G238D 0.198652625634507 G68C-N89D 0.198052168977481 I16D-L236D 0.19804310597648 I64C-T98D 0.197577177052151 A128D-G191D 0.197565750201947 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