[Wed Aug 24 18:22:26 2016] >Input file: TM4PJ0ATD.pdb >TM segments: A28-50_A114-132_A141-160_A195-217_A273-292_C50-69_C113-131_C157-177_C236-254_C269-287_C428-446_B19-37_B96-114_B138-157_B198-217_B236-253_B453-471_E19-37_D32-53_D110-130_D141-159_D195-217_D267-287_F19-39_I6-25_H29-47_K20-39_J11-29_M9-27_L14-32_R5-22_T4-24_Y19-37_X9-28_Z3-24_Z39-58_a28-50_a114-132_a142-160_a195-217_a273-292_c50-70_c113-130_c158-177_c236-254_c270-287_c426-446_b19-37_b96-115_b137-157_b200-217_b235-253_b454-471_e19-37_d30-53_d110-130_d141-159_d195-216_d267-287_f18-39_i4-24_h27-46_k20-39_j11-29_m9-27_l15-32_r5-22_t4-23_y19-37_x10-28_z3-24_z39-58 MAIDEN score = -893.9580 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- P20k-Q21y 10 Q21Y-P20K 10 T159C-I252C 8.18216138759067 T159c-I252c 7.32249326994076 V8R-A33E 1.84745986171665 A44d-I114d 1.7633414582385 I24M-I24m 1.75721241155528 A44D-I114D 1.62588462853619 I9M-I9m 1.6046997532378 I35y-I21z 1.57202631030621 W131A-C144A 1.52082075395176 I35Y-I21Z 1.49618072114746 A33e-V8r 1.43795123046576 W131a-C144a 1.37857087730037 A44a-I115a 1.32444831370323 A44A-I115A 1.31739238070907 L148B-I210B 1.25314839725862 L148b-I210b 1.24662406131606 L165c-I245c 1.24495335077696 L173C-I238C 1.2448614740723 L29L-I9T 1.24088932964165 I37f-W35e 1.2314182124669 I31F-L30E 1.2252352416998 L173c-I238c 1.22376399607212 I31F-F31E 1.22221823641555 W18R-I25E 1.22149345523482 I31f-L30e 1.21975008842378 I31f-F31e 1.20588231521695 L165C-I245C 1.19747492344262 I9t-L29l 1.16647794580564 F32F-F31E 1.15628306657955 I37F-W35E 1.14093296299543 I141b-I217b 1.13174050021302 F151B-I203B 1.12951982651775 I141B-I217B 1.12333529169987 F151b-I203b 1.11229479859334 I20b-H114b 1.09519370461388 W32a-F23i 1.09147432751949 L25L-I9T 1.08953637473476 L276c-H441c 1.08556614377086 A36f-W35e 1.0815091819977 I20B-H114B 1.07068290447943 I467B-I144D 1.06822105689871 I467b-I144d 1.06397086188668 L276C-H441C 1.05930434314493 A36F-W35E 1.05690942795213 I31F-I27E 1.05685634638933 F196D-M281D 1.04540662904642 F23I-W32A 1.04111466363789 I31f-I27e 1.03472115354644 F33A-Y126A 1.03365341787393 F33a-Y126a 1.0313576749282 I9t-L25l 1.01566073954864 F32f-F31e 1.01396492223179 F196d-M281d 1.01232807806984 I159D-V287D 1.01193185434692 V176c-I238c 1.00869407780472 I159d-V287d 1.00480416997129 F246b-F462b 0.995392638312756 V176C-I238C 0.99263031240837 F144B-I210B 0.990040522735195 F144b-I210b 0.983176256562012 F284C-F431C 0.977293935976348 I25e-W18r 0.970217330191261 F197a-F285a 0.959562842971376 E269C-A445C 0.951673953185544 V23z-I40z 0.950903210429829 V24k-I25y 0.948792246874847 Q130A-I143A 0.932596901961833 G34a-C125a 0.923093685871984 G128a-C144a 0.917845145171143 F197A-F285A 0.916955213502548 G34A-C125A 0.912560887181348 L25L-I13T 0.909098384234669 I25Y-V24K 0.908927727118575 L41a-F119a 0.906421700837863 L41A-F119A 0.904293243906415 F32F-L30E 0.903553124644137 L17L-L22M 0.900760654443816 F453B-V287D 0.900565068494461 G128A-C144A 0.894133843204306 L37D-F125D 0.890972873356111 L12z-L50z 0.886045816071936 V457B-I284D 0.872127904559592 V154d-I159d 0.871205294943175 P11R-P28E 0.860668432886942 F5Z-L57Z 0.854199750114016 L12Z-L50Z 0.85192797946207 V154D-I159D 0.849539435788722 A152A-W284A 0.847364538846947 L37d-F125d 0.846708588288438 A38f-W35e 0.843424985649784 W142A-A216D 0.840503181630079 L22m-L17l 0.837893078077562 W142a-L442c 0.836732810811704 W48d-L110d 0.834018736858019 I20b-W115b 0.83007085661756 L275A-H214D 0.82840956406571 L442C-W142A 0.825611903157893 L272c-H444c 0.82348917445499 L175c-H237c 0.821725001374724 L175C-H237C 0.820261559103868 W131A-I143A 0.819588424719746 V457b-I284d 0.817786358798948 P28e-P11r 0.812191520125449 W48D-L110D 0.811259377717657 I13t-L25l 0.810580843451069 F5z-L57z 0.80798620262648 V23Z-I40Z 0.807905637755528 W443C-V145A 0.806977924196128 L276c-F437c 0.80121660077342 A152a-W284a 0.800727859941958 T292a-F431c 0.800553063293336 F431C-T292A 0.799630989953746 L275a-H214d 0.799539937858401 F33A-C125A 0.796840877488139 F246B-F462B 0.793323855387504 L122D-I150D 0.789131793869265 L276C-F437C 0.788142118240833 W63C-L119C 0.785094686019546 L122d-I150d 0.784300365286668 L272C-H444C 0.781606196396464 F33a-C125a 0.781165844898096 I24L-V15M 0.780707688306049 G35F-W35E 0.778921213629304 G35f-W35e 0.771191558856345 W142a-A216d 0.77040805316371 V15m-I24l 0.765523813833659 I22e-W18r 0.765282710557309 L24b-L107b 0.763333790769549 L22L-I13T 0.762442801021138 A209a-V204d 0.762274488436798 S155D-I284D 0.761655525788833 W18R-I22E 0.761303858492282 S36B-I101B 0.759834666129485 V35a-F15i 0.759502645931377 C212a-L275a 0.758780686897674 F32f-W35e 0.757829934665307 C12t-L25l 0.757154783631691 F32f-L30e 0.755992397212299 C212A-L275A 0.755343754297984 W131a-I143a 0.754693368192462 S155d-I284d 0.753984625246361 L21k-M24y 0.75327722544921 Q130a-I143a 0.750814011451276 F19I-V35A 0.746880105147456 F5z-V53z 0.745506918976186 W48d-I114d 0.742630817913611 F15I-V35A 0.741471602982063 F5Z-V54Z 0.740726294986128 A21b-W113b 0.739304557771399 L9z-V54z 0.738508701707912 F246B-F463B 0.738024642925224 W63C-L25K 0.736699507807074 I13t-L22l 0.7356579340992 V54C-L125C 0.73311943974943 A21B-W113B 0.72959441437439 L30e-V12r 0.728338419822418 M126d-F146d 0.726575384977389 V54c-L125c 0.72577440174495 V12R-L30E 0.72552880269187 V35a-F19i 0.724422629888199 M126D-F146D 0.718923549137937 A38F-W35E 0.717243486892155 F206A-V204D 0.716640224954593 V145a-W443c 0.71622383999914 P11R-L30E 0.71620823232645 V38K-T15J 0.713257384276487 L17L-I23M 0.713248034295218 V38k-T15j 0.712714816112584 S36b-I101b 0.712368488362131 Q21Y-V24K 0.711947970039829 W278A-A212D 0.709749182672506 F206a-V204d 0.707163155228905 F144B-L214B 0.704846236881605 T45A-I115A 0.702243943849605 L30e-P11r 0.700838053168107 W48D-I114D 0.700532850105564 F246b-F463b 0.693490252155538 V205a-L205d 0.692902941226251 L276C-L438C 0.692003830021084 W63c-L119c 0.689940134751341 F32F-W35E 0.688638180609146 W468b-I144d 0.685615998929 L25L-C12T 0.685507490103629 V38K-W11J 0.685190209691231 M37a-A123a 0.683829093676904 M37A-A123A 0.683403461062231 A243b-F463b 0.682740741497605 A36f-F37e 0.682467531575588 G169c-I240c 0.681090481779861 I36A-G122A 0.680562554664151 V202a-G282a 0.680251484310032 L24B-L107B 0.679061017857115 H215A-L272D 0.678670471046152 G204a-V280a 0.67814969997781 F14m-A28l 0.6775242409044 L276c-L438c 0.676096630511566 L24b-A110b 0.675005964081342 L16t-Y18l 0.673030183611867 F196D-I284D 0.672999261870301 F196d-I284d 0.671611485781141 L43d-I114d 0.67040352522138 I116a-F158a 0.670312937026906 F144b-L214b 0.670059026177849 G35b-V96b 0.670047804936645 L27j-A38f 0.669632402034749 I116A-F158A 0.669558813307217 A209a-L205d 0.669090703822558 L279C-A434C 0.668427078521406 I23m-L17l 0.668407861637884 A123A-L151A 0.66761546034859 A209A-L205D 0.666970309922581 F284c-F431c 0.665411500755872 F273a-A216d 0.665391580266506 A243B-F463B 0.665362381795613 A151D-W280D 0.665352724068016 A151d-W280d 0.665216816502055 G203d-V276d 0.664989149073514 C144a-L442c 0.664225449655493 A28L-F14M 0.663095935256247 A31b-L103b 0.662600249243808 L24B-A110B 0.662515198789446 A36F-F37E 0.662074874677444 F5z-V54z 0.661132219934683 C47A-L114A 0.66103382191394 F62c-L119c 0.660331089725757 Q130A-C144A 0.658211333211533 L161c-H251c 0.656018229429981 G203D-V276D 0.655255934953469 A31B-L103B 0.655032048066603 Q130a-C144a 0.655030585013555 V38k-W11j 0.654304220867423 F273A-A216D 0.653196720367116 G204A-V280A 0.653024399123065 V152D-A283D 0.652923593709151 W131a-G446c 0.650964632862313 V205A-L205D 0.649808773864469 I60c-S122c 0.649488931973305 G35B-V96B 0.649282777964535 V205a-G282a 0.648097344491257 V7R-A33E 0.647939140451308 M67C-L25K 0.647593150572706 V30a-R129a 0.646176450085894 L9Z-V54Z 0.646093704589786 G34e-V8r 0.645914103714159 F37e-V8r 0.642533099073226 V152D-W280D 0.642495593878504 L18I-W32A 0.641933069629548 G446C-W142A 0.641914787450749 G162C-W250C 0.640814774624754 L43D-I114D 0.64077989798112 W278a-A212d 0.638921678453285 T26e-A15r 0.634762399448816 F284C-H430C 0.634295348442402 Y18L-L16T 0.633054377803547 W468B-I144D 0.632842030520743 A123a-L151a 0.632665340600793 T45a-I115a 0.631400516220384 V22k-F70c 0.631124692818067 L279c-A434c 0.631076551968088 C47a-L114a 0.628938814236649 L210A-P275D 0.628541546240734 I36a-G122a 0.62848669746135 L210a-P275d 0.628414017888672 G162c-W250c 0.62753919990856 F196D-S282D 0.626887277974018 G47d-F113d 0.626588344156486 I60C-S122C 0.626077892341253 A66c-V116c 0.623180396707421 G204A-V281A 0.621487227975281 A44A-I116A 0.621115442101291 L12Z-W47Z 0.620805933944583 A172C-I240C 0.620357613153963 G65c-V117c 0.619922352958384 G140b-F215b 0.619669096036556 M281c-A434c 0.619297743288739 S153a-I290a 0.617838075881262 A28B-L103B 0.617282586123379 G140B-F215B 0.616319088538488 I37f-T53d 0.616292157705396 F62C-S122C 0.616114270129677 F38d-G121d 0.61389891439862 G446C-W131A 0.613875208023323 G47D-F113D 0.613842090645911 F38D-G121D 0.613774746191426 L460B-A151D 0.613551411813809 F32F-A33E 0.6132314664427 G216a-F270d 0.613166322870487 F274a-G215d 0.612790492379659 F274A-G215D 0.612617896824071 G216A-F270D 0.612503935812085 V152d-W280d 0.612486907429097 V8R-F37E 0.612134218938166 Q21Y-D23K 0.609824480592462 F5Z-N58Z 0.608086790168825 A55C-F127C 0.60598073320299 F32f-A33e 0.60586829664472 F62c-S122c 0.605274039459033 V202A-G282A 0.605270784504023 A57c-L125c 0.604440299279998 A172c-I238c 0.604068361365234 W142a-G446c 0.603194215554483 F130D-A143D 0.602766381047338 V145a-L442c 0.601478724842952 S153A-I290A 0.601156463554988 A64C-L119C 0.59992623672639 F155a-I160a 0.598182492477992 V8R-G34E 0.59808883630331 V28F-L30E 0.597872870585517 M67C-P26K 0.597400455556996 V8R-L36E 0.597203444185842 G204a-V281a 0.595001083329731 L460b-A151d 0.593830936295161 I36a-F19i 0.593811240321524 F196d-S282d 0.59317291256556 G207A-P279A 0.592569628387545 S280C-F437C 0.592538152459277 M25b-F108b 0.592024015756682 M281C-A434C 0.590932805207375 F130d-A143d 0.590780058920812 F250b-A459b 0.590252622202361 I166C-C244C 0.588389973419432 A64c-L119c 0.588354764075778 M25B-F108B 0.58767097677596 T287C-T428C 0.587632267417797 V205A-G282A 0.58731440904686 A31Y-I21Z 0.586969294023993 I37F-T53D 0.585953095631637 V205A-A208D 0.585189830202484 L442C-C144A 0.584140001521217 V21e-N22r 0.583438028514157 F19I-W32A 0.583344723236964 V27M-I24m 0.582302832787115 L143B-I210B 0.582200548812533 L238b-H469b 0.579583484147394 F196D-A283D 0.579549442000813 G34a-Y126a 0.579074512156317 L238B-H469B 0.579035535915513 F62C-L119C 0.578065437380042 F139B-G213B 0.576909895390179 I24M-V27m 0.576149600563873 Q130A-P141A 0.575910734893441 G206D-P275D 0.57507201498774 W443C-W142A 0.575041080930191 H198A-F285A 0.573831099437633 V16I-I36A 0.572656073522593 A38F-L27J 0.57204871342633 G34A-Y126A 0.571269025540792 F19I-I36A 0.569007740587637 G206d-P275d 0.56508617894234 V157a-I290a 0.564201087489172 V157A-I290A 0.561791621344212 A213A-V274D 0.561436648419748 A55c-F127c 0.561004977134369 V30A-R129A 0.559710557725941 A28b-L103b 0.558466901435615 A57C-L125C 0.557741814081082 F33a-F19i 0.557512947886153 W142a-W443c 0.556272337783392 G58C-V124C 0.555638342122543 T287c-F431c 0.55562680662006 W32a-L18i 0.554311291006567 I166c-C244c 0.553315315043749 L161C-H251C 0.553274462902682 I242b-G465b 0.553230878949593 T287C-F431C 0.552488249698019 S124A-L151A 0.552418282360522 I467b-S147d 0.551773300463711 S239B-I467B 0.551710610063058 S280C-L433C 0.551607163883518 F28j-A38f 0.550948849445742 M214a-L272d 0.549741492086038 G208a-W278a 0.549281852751651 M37a-L121a 0.549075890838647 V252b-H455b 0.547845455942327 H215a-L272d 0.547763519106459 L69C-D23K 0.547539297619005 W32a-F19i 0.547516038907813 L200a-F285a 0.54740729348144 T236b-H469b 0.546979542713922 F19I-F33A 0.54697526066891 F41Z-L46Z 0.545817293455536 F284c-H430c 0.545527697857302 I242B-G465B 0.543715290305576 A213a-V274d 0.540463867205952 S273C-L442C 0.539769880023876 F155A-I160A 0.53883308269732 A276a-I213d 0.538636737183529 A172C-I238C 0.537977710918461 A44A-L114A 0.5369007560168 S239b-I467b 0.536523564411453 L200A-F285A 0.536336613972875 A249b-F458b 0.535886430202057 M214A-L272D 0.53454539284997 L12z-W47z 0.53420487770844 R14L-I23M 0.534065898489751 A31y-V20z 0.533290136087457 I22e-A19r 0.532822740341564 F206a-A208d 0.532741486535301 L165c-C244c 0.531568827763872 S124a-L151a 0.53123924424437 A249B-F458B 0.531084964059394 A253B-F453B 0.529989941341067 L36e-V8r 0.529154709636445 F197A-L288A 0.528499498537754 F62C-A123C 0.528135507307354 G207a-P279a 0.527914307436196 A243B-F462B 0.527811465386236 T236B-H469B 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