[Wed Aug 24 17:30:00 2016] >Input file: TM4QTNCGD.pdb >TM segments: A20-35_A40-57_A61-75_A85-103_A130-145_A158-175_A179-195_A207-223_C490-506_C510-526_C532-545_C555-573_C600-615_C627-644_C649-665_C676-692_B255-271_B275-291_B296-310_B320-338_B364-379_B393-410_B414-430_B441-458 MAIDEN score = -201.3652 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- C52A-N63A 5.52030762481519 C522C-N533C 1.30399574099478 C287B-N298B 1.29171364096032 T426B-Y448B 1.23035238988032 I420B-L452B 1.18513440492347 Q405B-L410B 1.06224765198874 I662C-L680C 1.05702522234279 W184A-I189A 1.024230588983 C287B-Y299B 1.00220333082132 A404B-E414B 0.950045381841548 Y534C-F569C 0.934762483003831 C522C-G537C 0.930043748454243 A545C-M557C 0.928297993681744 C52A-Y64A 0.922160868435707 L300B-Q333B 0.911358843139773 A169A-E179A 0.907859780584896 I192A-Y213A 0.906939554982379 Y534C-F572C 0.876237390152276 I185A-L217A 0.870405348338512 C52A-G67A 0.867594293673028 Q170A-L175A 0.861763588440525 D394B-L425B 0.860630654930382 I427B-Y448B 0.85826449400892 Y64A-F102A 0.851880545345654 C287B-G302B 0.849974036487723 C522C-Y534C 0.845469032165207 I655C-L687C 0.844780515229998 V71A-Y94A 0.843320910864888 W430B-L444B 0.839993592380558 V541C-Y564C 0.838164943576393 L23A-W365B 0.835737870879634 A169A-W184A 0.832159183603022 A639C-W654C 0.830989010991032 A404B-W419B 0.82982587885501 A404B-W416B 0.824520268438376 F33A-W40A 0.822287536698401 A169A-W181A 0.818749857504357 I427B-L445B 0.8100963999719 F268B-W275B 0.80555428499566 A639C-E649C 0.794091273204971 W195A-L209A 0.787750742529376 W130A-L493C 0.780309241099966 W665C-L679C 0.758964172482516 D394B-L429B 0.758798325891057 W184A-L217A 0.74727226388252 A639C-W651C 0.744404838490863 V523C-N689C 0.71912840596624 L41A-L73A 0.717961813083183 V291B-N298B 0.717785498251687 V288B-N454B 0.715220970408288 V635C-L653C 0.714699361230732 V289B-N454B 0.714666084138404 D629C-L664C 0.714629333277113 N188A-V214A 0.71308280569218 V526C-N533C 0.713046594689834 W419B-L452B 0.712877628918127 V524C-N689C 0.712362027732268 L407B-E414B 0.709469210908189 T141A-V504C 0.709416801411293 A75A-M87A 0.707801298932091 F503C-W510C 0.697784794282202 I192A-L210A 0.696661445257934 N658C-V684C 0.695054642494846 L303B-Y329B 0.692670894788211 V53A-N63A 0.69202030104957 V288B-N298B 0.691707945208877 L538C-Y564C 0.688358108970598 V34A-T376B 0.687857147808623 V54A-N219A 0.687739484613003 V56A-N63A 0.686642713803669 V523C-N533C 0.685801313309305 A75A-M88A 0.683501940138191 G555C-V633C 0.680581377842886 N423B-V449B 0.679224231079984 W654C-L687C 0.678649904708848 A545C-M558C 0.673002454512228 V400B-L418B 0.67264457432671 V165A-L183A 0.672128614165757 F55A-I61A 0.666967224526396 T627C-F270B 0.666523784661567 V53A-N219A 0.664439763495623 A281B-L444B 0.663810601639996 G519C-S540C 0.657957168131341 V403B-Q415B 0.657943682546009 Y64A-F99A 0.657615400782717 V291B-G458B 0.650674481355929 T611C-V269B 0.648542430853982 A75A-L91A 0.648472693127733 V306B-Y329B 0.644833275693401 G49A-S70A 0.643887277814157 L68A-Y94A 0.643039256382014 A545C-L561C 0.64214770250572 A310B-M323B 0.639464009914182 G284B-S305B 0.637299190467686 Y299B-F337B 0.634829304632378 W40A-A45A 0.634564098725087 L642C-E649C 0.633797689162769 A42A-L209A 0.632150684588631 G85A-V163A 0.629612251175005 W130A-V490C 0.628676994708501 A310B-L326B 0.626822516306946 V20A-W365B 0.626346206513783 A516C-L679C 0.626164958475973 A46A-L209A 0.620470399176177 V634C-A639C 0.619608694484995 I662C-Y683C 0.618493886874424 L326B-P331B 0.61836704770107 W365B-A370B 0.616975400721729 I296B-F337B 0.615188056630739 A652C-W256B 0.613940003270506 A277B-L444B 0.610428389717525 L91A-P96A 0.601853879528933 V168A-Q180A 0.592948438942905 A42A-L73A 0.592287918367504 A516C-P678C 0.591898107707935 F290B-I296B 0.591805657662628 W651C-V691C 0.584431393259497 G57A-Y222A 0.583499929216099 S307B-L326B 0.581259860495254 W416B-V456B 0.576116081481813 W181A-G223A 0.573398835064709 A512C-L679C 0.57304363368093 W181A-V221A 0.572371527240584 L535C-Q568C 0.570762627336492 V53A-Y216A 0.570677667427699 V288B-Y451B 0.569305443280501 V523C-Y686C 0.569042704081342 G284B-V289B 0.566067969173811 C287B-L300B 0.56265274159939 L68A-V95A 0.559442875558037 W275B-A280B 0.557913273196995 A45A-I69A 0.554910224093094 G320B-V398B 0.551310333609751 W416B-G458B 0.544871778546492 V638C-Q650C 0.540931371264099 W665C-L680C 0.53927107525851 S72A-L91A 0.532383697483513 S166A-L183A 0.532292985740612 V608C-A265B 0.519621435714189 A182A-W491C 0.517405273814446 S401B-L418B 0.517227451284091 E321B-V398B 0.513764433419368 L561C-P566C 0.510990648782099 D159A-L190A 0.510449818370498 Y299B-F334B 0.508512228836031 L303B-V330B 0.505390527583951 E321B-D394B 0.503842110903741 N423B-Y451B 0.502645443662978 G49A-V54A 0.501236984377446 W600C-V255B 0.499219877510581 W510C-A515C 0.494213521493602 Y309B-N325B 0.493751540406087 L496C-V517C 0.49228850187783 N658C-Y686C 0.490220049336194 W419B-S455B 0.489820047121668 A277B-Y309B 0.489371288633105 W184A-S220A 0.48911741145434 A42A-Y74A 0.488377279389479 A404B-Q415B 0.485512972325734 A30A-V373B 0.48492363777486 W654C-S690C 0.479615561510611 W130A-A135A 0.479126811508951 V56A-G223A 0.478379841905209 L538C-V565C 0.478076009291141 L276B-L308B 0.476429458580786 V34A-S377B 0.475716060758779 S607C-V266B 0.47220842565583 W600C-A605C 0.471867532754898 S137A-V501C 0.4704237908556 L23A-L366B 0.469989676588542 S542C-L561C 0.463970944799514 I285B-Y451B 0.462185054296132 L65A-Q98A 0.461519715517925 I189A-Y213A 0.461275789608119 L211A-Y216A 0.460504207008804 V541C-L561C 0.457067819070275 T611C-V266B 0.455740611841843 Y64A-Q170A 0.454949636790856 S636C-L653C 0.452526835417177 W665C-S676C 0.443310903424805 W430B-S441B 0.442839316039586 L261B-V282B 0.441410404258555 V97A-F102A 0.439930729678407 L51A-F66A 0.439275413381009 L26A-V47A 0.437536279115241 F495C-I520C 0.435408676564546 A512C-Y544C 0.434944986301331 L511C-L543C 0.434274800132605 Q29A-V47A 0.434202032227196 S166A-V187A 0.43410536870523 F55A-S62A 0.432256842144706 L68A-Q98A 0.431849843197247 A46A-M212A 0.431847473830531 F25A-I50A 0.43025091562398 I50A-Y216A 0.4279412107428 Q264B-V282B 0.426219838796452 A516C-M682C 0.426129593140487 S401B-V422B 0.425502396197004 V635C-W654C 0.424297759012697 A280B-I304B 0.423770518208489 V138A-A500C 0.422553231047139 L286B-F301B 0.422266192706499 L538C-Q568C 0.420974738883622 A280B-V306B 0.420959536287608 E86A-V163A 0.419308890181266 A652C-V255B 0.418681892045733 V266B-N271B 0.418246626666797 Y544C-N560C 0.417223013123016 F290B-S297B 0.41672666375262 W21A-A417B 0.415520503568763 V604C-L258B 0.415300442696843 A515C-P678C 0.414490946961471 I659C-Y683C 0.414152043020668 S636C-V657C 0.41414049848258 T397B-L425B 0.41370792545138 E86A-D159A 0.413653888962725 V635C-V656C 0.411764818544036 I427B-M447B 0.41164229375377 V168A-L183A 0.410339185711457 V403B-L418B 0.410298321835411 L559C-V633C 0.409489791302721 A281B-P443B 0.407135483882152 V44A-L73A 0.407008886916449 I520C-F525C 0.405640656399103 V32A-V396B 0.40547070810009 S372B-V399B 0.404348218881251 N188A-Y216A 0.404262852072788 V502C-L677C 0.403298128488271 I520C-Y686C 0.402294982282058 S513C-T518C 0.400665665618599 A45A-V71A 0.399659775131493 S43A-T48A 0.399213143188259 D159A-L194A 0.399124703946431 A280B-P443B 0.399030629310881 A515C-I539C 0.397168927941811 L521C-F536C 0.396070297448336 W654C-M682C 0.395098021766229 A515C-V541C 0.394825826353922 W419B-M447B 0.393617862163077 I61A-F102A 0.393616326846885 S278B-T283B 0.392305095551968 S612C-V269B 0.391327848601418 N325B-P331B 0.389468892371186 D629C-L660C 0.387058841574753 L324B-V398B 0.386106122799716 I420B-Y451B 0.385130861822616 V400B-W419B 0.380964249845859 N90A-P96A 0.380457663175406 V165A-W184A 0.378637772676111 L303B-Q333B 0.378023611552528 N560C-P566C 0.377776288511427 L677C-M682C 0.375114277710819 G302B-Q333B 0.374163942355999 W256B-M447B 0.37308459142157 Y299B-Q405B 0.372339228446205 Q499C-V517C 0.371965578527508 W430B-L445B 0.371458461946124 T632C-L660C 0.370165842720557 S70A-P208A 0.36613141139996 V279B-L308B 0.364351691049616 V71A-L91A 0.364267199656878 V34A-V373B 0.36378462906627 L393B-L425B 0.362968317549225 I285B-F290B 0.362349707198531 C52A-L65A 0.361399454076647 G555C-Y564C 0.360637814343316 T661C-L680C 0.358907952716344 L286B-V291B 0.357042021920996 V20A-Y222A 0.356626722030641 Y329B-V400B 0.356449137111609 T518C-F536C 0.356131574039411 L496C-V501C 0.355390526519398 S540C-P678C 0.354807958003661 A512C-L543C 0.354769283217698 W184A-M212A 0.353834114863513 A310B-L324B 0.353030011919525 M24A-V369B 0.352582365962342 G284B-Y329B 0.352205368208838 S305B-P443B 0.352097969740099 A45A-P208A 0.349936138890353 Y74A-N90A 0.349719868276769 V138A-G497C 0.349488748507953 V631C-V266B 0.348847711561083 Y329B-V399B 0.347720228416806 A277B-L308B 0.347014715799727 V134A-M494C 0.346496328164154 G519C-V524C 0.346309351227148 L41A-Y74A 0.346200803185005 A500C-A512C 0.345829867753924 S142A-V504C 0.345199249033507 P566C-Q640C 0.344624939693721 L207A-M212A 0.344522396353793 F525C-S532C 0.34327322858734 I50A-F55A 0.342588308111727 T627C-V267B 0.342380806331308 A512C-L677C 0.341641095960787 A45A-S72A 0.341330305912512 V514C-S540C 0.340239183413714 A512C-S676C 0.339016558271959 G320B-Y329B 0.338916590466483 W651C-V657C 0.338521905352946 T141A-V501C 0.337381568527434 Y544C-M557C 0.336796240191846 V186A-Y686C 0.336706518512872 G537C-Q568C 0.33665787914417 L442B-M447B 0.336637375754895 M408B-V456B 0.336433635386711 M259B-V269B 0.336433635386711 M173A-V187A 0.336433635386711 L51A-V56A 0.336295458352313 G67A-Q98A 0.335761205742353 S305B-P331B 0.335546318040302 G519C-Y564C 0.335469423408674 A46A-S70A 0.33530799376534 A639C-L653C 0.335011063500336 Y309B-M322B 0.334871003048196 V502C-M685C 0.334261906982838 V161A-V501C 0.334104212241886 T162A-L190A 0.333974612914605 V279B-S305B 0.333720871235734 Q650C-Y686C 0.332427467099937 A281B-S305B 0.33199916074746 Y64A-V93A 0.331045990055978 M323B-V403B 0.330756330890649 Y216A-V421B 0.330586808640399 G519C-F536C 0.32943221933885 L559C-Y564C 0.329380636753767 V267B-L442B 0.329042369567754 V32A-W40A 0.327868639901279 D629C-V266B 0.327494224895908 G302B-A310B 0.327422320697891 V631C-M447B 0.327304180644827 S137A-V164A 0.327085549879063 Q364B-V369B 0.325715635386312 G537C-A545C 0.325600655337192 A515C-L543C 0.323604280603826 M322B-L327B 0.32339509739017 M24A-V398B 0.32270655311447 I424B-Y448B 0.321700772618617 A516C-S540C 0.320883173198095 Q568C-A573C 0.320349015854139 P96A-Q170A 0.320348064478226 Q98A-A103A 0.320144082808265 M643C-Q650C 0.320005490429841 I285B-M450B 0.318462318568825 W419B-V456B 0.318349669981624 Q180A-Y216A 0.318280657737031 E556C-D629C 0.317789912360875 E321B-L326B 0.317348816561186 A277B-L442B 0.31717050277973 Y94A-V165A 0.317077657681988 S540C-P566C 0.316471309050473 G67A-A75A 0.315127646990367 Y534C-Q640C 0.314278638012916 V306B-V330B 0.314232448882513 A281B-M447B 0.312673247478738 M259B-N454B 0.312415880281335 A75A-L89A 0.31240527136957 W256B-L261B 0.311827676678899 I167A-M173A 0.311728531176653 G57A-C218A 0.311727412061612 M24A-N219A 0.311208112946269 Q333B-A338B 0.310821141565176 L89A-V163A 0.310246319895743 T191A-Y213A 0.308868990215704 V638C-L653C 0.308691808996278 S372B-A404B 0.308471461285097 C52A-V97A 0.308451229365152 A515C-L679C 0.308264558890968 C218A-V456B 0.307239158185902 I520C-M682C 0.307069173838466 Y74A-M87A 0.30701110533779 V32A-L207A 0.306979123138813 G27A-V373B 0.305560209545598 S607C-A639C 0.305417782693114 V134A-L493C 0.305291043470703 V501C-N506C 0.305261896972032 G610C-V634C 0.304106654268992 V567C-F572C 0.303320417469916 G537C-V565C 0.303111313029646 M88A-V168A 0.302906156244802 W491C-M682C 0.302649145007652 V502C-P678C 0.302311167875379 V604C-M259B 0.301990366927153 L23A-V369B 0.301792761927494 G49A-F66A 0.301086627716685 C287B-V332B 0.300862999159779 C522C-V567C 0.300617622248111 L366B-A371B 0.300189439866698 G320B-S401B 0.299826103606958 M494C-N689C 0.299792623884876 L511C-A516C 0.299003238082555 W195A-L210A 0.298236338038621 A42A-L207A 0.298225355812211 L131A-L493C 0.296858602874775 A545C-L559C 0.296679048531773 Q650C-M259B 0.296675068653366 Y94A-V164A 0.296104748483677 G57A-Y64A 0.29591130759027 V44A-S70A 0.295657146254999 C522C-L535C 0.295637275774849 S142A-A500C 0.295149631384948 A605C-S612C 0.292315440991159 V570C-I644C 0.292149636422076 A45A-L209A 0.292114800189784 V335B-I409B 0.291176271069991 V56A-I61A 0.291104805034461 V635C-V657C 0.290547415421862 S137A-G160A 0.290346960789096 G302B-L326B 0.290281741297005 I28A-C218A 0.289811745987107 G497C-L681C 0.289323956300806 S307B-V330B 0.289091652814512 M212A-C218A 0.288883515051662 A281B-L446B 0.286563537301396 M322B-V422B 0.286489385570802 V291B-I296B 0.286370641827692 L41A-A46A 0.285955268460457 G630C-N271B 0.285926994308859 V631C-V267B 0.285190687788996 G160A-N506C 0.284949320203114 M87A-L92A 0.284731434954976 G27A-L211A 0.283122342187237 V34A-V374B 0.282885969126314 V134A-V168A 0.282807246781988 V138A-V164A 0.282268781838298 V608C-V634C 0.282237115909667 I185A-Y216A 0.282182932566259 L601C-A606C 0.28194314479214 L132A-S137A 0.281819265946885 V330B-V335B 0.281573333927388 S612C-A265B 0.281382740603108 L89A-Y94A 0.280600289678684 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