[Wed Aug 24 17:45:39 2016] >Input file: TM4RDQATD.pdb >TM segments: A27-50_A65-84_A235-258_A269-289_C27-50_C62-84_C238-258_C269-289_B27-50_B65-84_B235-258_B269-289_E27-50_E65-84_E235-257_E270-289_D27-50_D65-84_D237-258_D270-289 MAIDEN score = -159.5148 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C69A-A250A 8.60318189166532 C69B-A250B 7.45692698541365 C69E-A250E 7.43997205083224 C69D-A250D 6.70994652103843 C69C-A250C 6.4638612092769 I28E-V235A 2.19676987553987 V235B-I28A 2.17050746527404 V235E-I28D 2.16054155288215 C251B-T277B 1.13883232291877 C251E-T277E 1.13336733950795 C251A-T277A 1.12955095023209 C251C-T277C 1.12474383504076 C251D-T277D 1.12461377827348 I76B-I76C 1.1216524399334 I76E-I76A 1.11381313054131 I76C-I76D 1.0931836088525 I76B-I76A 1.08921788639087 L32C-K289C 1.08533998331986 I76E-I76D 1.0845105230552 L32B-K289B 1.08280718202377 L32A-K289A 1.07420942600278 L32E-K289E 1.07371477087305 L32D-K289D 1.06790903322423 W287E-V239A 1.03745503738761 W287C-V239D 1.03157728516477 V239B-W287A 1.03074159961125 W287B-V239C 1.02511521931421 V239E-W287D 1.02015054950407 Y72B-F247B 1.00605912932016 Y72C-F247C 1.0054566350216 Y72D-F247D 1.00544591330631 Y72A-F247A 1.00480637032175 Y72E-F247E 1.00465200934756 A66B-R255B 0.987843654306301 A66E-R255E 0.986285991798788 F282B-V239C 0.985892466601993 Y50C-F62C 0.981924617396765 V239B-F282A 0.981021806732414 F282C-V239D 0.978576460402137 F282E-V239A 0.977112407079647 V239E-F282D 0.976358113091389 A66A-R255A 0.975558404552896 A66D-R255D 0.971964229553858 A66C-R255C 0.951173860243497 F276E-Y72A 0.942213284197095 Y72B-F276A 0.928896156745514 Y72E-F276D 0.920222701908543 F276C-Y72D 0.913256518494753 F276B-Y72C 0.880524346893685 V235E-S27D 0.869088125630912 S27E-V235A 0.841230127616145 V235B-S27A 0.819133771101942 F283B-V240C 0.801251354682677 A66C-I253C 0.787183903073281 A66D-I253D 0.784851143133691 A66A-I253A 0.772863363070897 A66B-I253B 0.770307794287241 N70D-I253D 0.763417616403774 N70B-I253B 0.763024479825171 N70C-I253C 0.759667412059998 L258A-D270A 0.758344975290702 A66E-I253E 0.758260401036338 L258C-D270C 0.757629612019354 L258D-D270D 0.757416613550487 L258B-D270B 0.757200985882963 N70A-I253A 0.757199548676311 N70E-I253E 0.755751633760839 V240B-F283A 0.742269133689899 F283E-V240A 0.737144437926281 C69B-I253B 0.73448667071991 F283C-V240D 0.734184595219194 C69D-I253D 0.733029188170176 C69C-I253C 0.732663223753974 C69A-I253A 0.732640153682705 C69E-I253E 0.732083632193019 V242E-F283D 0.714927396858316 F283B-V242C 0.710416265139644 V242B-F283A 0.710223034211615 F283E-V242A 0.710218726553766 F283C-V242D 0.709956500873589 L269C-L65D 0.707594797378758 V244A-Y284A 0.707138307449584 V244B-Y284B 0.701666798096909 L65B-L269A 0.700588587287616 V244D-Y284D 0.69874331619637 V244C-Y284C 0.696065320561607 L269B-L65C 0.692808736469706 V244E-Y284E 0.692686329940227 V240E-F283D 0.692040174654565 F282E-W287E 0.69129309050237 F282C-W287C 0.68935605418513 F282B-W287B 0.688047722339203 F282D-W287D 0.687827825250831 F282A-W287A 0.682759019120368 V235B-K289A 0.668759022088217 I28E-K289E 0.666669587432812 L32C-A285C 0.665873605044002 I28C-K289C 0.665719732139096 L32A-A285A 0.665665736675001 L32B-A285B 0.665352237658289 I28B-K289B 0.662877013452457 L32D-A285D 0.662845726149787 I28D-K289D 0.661610914180295 K289E-V235A 0.661367658324807 L32E-A285E 0.661339014023484 I28A-K289A 0.660896475745302 F276B-C69C 0.660549410588888 F276E-C69A 0.660414835906101 F276C-C69D 0.660255288717286 C69E-F276D 0.658074007973609 L269C-Y68D 0.657547822007347 Y68B-L269A 0.655869806625251 C69B-F276A 0.655011495588063 L269B-Y68C 0.649794924005217 F257C-F272C 0.641740578626648 F283B-V239C 0.641590431771399 F257E-F272E 0.640034798498321 V239B-F283A 0.639874438175292 F257A-F272A 0.638476346301753 P77E-L75D 0.636490823370464 F257D-F272D 0.635105630544931 F283E-V239A 0.633240975539674 F283C-V239D 0.632786605223994 F257B-F272B 0.628505332849811 V235E-K289D 0.62773414392375 V239E-F283D 0.625015391982745 L75C-P77D 0.622759235104361 F281E-G286E 0.621659184715653 F281D-G286D 0.621499354412272 F281A-G286A 0.621243784589544 F281B-G286B 0.620815514552558 F281C-G286C 0.619948994432058 L82A-L288A 0.618820850206881 F80B-G83A 0.618772378527358 L82D-L288D 0.617947630186544 G83C-F80D 0.608950206210722 F80E-G83D 0.608587045246266 L75E-P77A 0.606269119562736 L82C-L288C 0.606216473825256 L82B-L288B 0.602399561022204 L82E-L288E 0.60025753600878 Y43C-I253C 0.599828914122792 G83B-F80C 0.59931806276737 P77B-L75A 0.596955813585817 Y43A-I253A 0.596888856648623 Q256E-P274E 0.59272907194991 Y43B-I253B 0.590215400326335 Q256C-P274C 0.590019369191086 Y43D-I253D 0.588235897206295 G83E-F80A 0.586480746099172 A250C-R255C 0.584506295707175 Y68C-G254C 0.583912089204677 Y43E-I253E 0.58339740756498 Q256B-P274B 0.58305522030167 Q256A-P274A 0.580120015534016 Y68E-G254E 0.579590407783346 Q256D-P274D 0.579472029681805 Y68B-G254B 0.578664267328333 Y68D-G254D 0.57585652042318 V239E-I28D 0.575511216466182 I28C-V239D 0.574793772877335 Y68A-G254A 0.573879655633752 I28B-V239C 0.572601887408689 A250A-R255A 0.570466164090272 A250E-R255E 0.570255688220724 I28E-V239A 0.567705937998849 A250D-R255D 0.565395266197607 L75B-P77C 0.563671451877822 V239B-I28A 0.558928243415798 V78B-F247B 0.557282315059787 A250B-R255B 0.556619767071027 F257A-L269A 0.554585027476234 F257B-L269B 0.553130383885087 F257C-L269C 0.551695487446742 A73E-F248E 0.549429742600583 V78C-F247C 0.548993754724513 F39A-L252A 0.547186325284767 F39E-L252E 0.546216512955559 A73A-F248A 0.544313634541703 F39B-L252B 0.543817431955499 V78D-F247D 0.542852399601001 A73D-F248D 0.542150013721991 A73B-F248B 0.540926655713167 A73C-F248C 0.540837908836609 F39C-L252C 0.540207940275787 F39D-L252D 0.538399608811898 G286E-V235A 0.532478267891536 V235E-G286D 0.531705719697624 V78A-F247A 0.527541711218067 Y236E-W287D 0.527469212164044 V78E-F247E 0.525485411594742 L32D-F282D 0.525314072793157 L32C-F282C 0.525175541901602 L32A-F282A 0.524895518111293 L32B-F282B 0.52392839807448 L32E-F282E 0.523913031134312 E74B-F247B 0.523352549643843 E74C-F247C 0.522644754057982 W287E-Y236A 0.522318738005037 V81B-A243B 0.520160143018069 E74E-F247E 0.519546572160469 R255E-P274E 0.519323817356511 F257A-L271A 0.519294659693034 E74A-F247A 0.518865174849933 E74D-F247D 0.517618125613092 F257E-L271E 0.516985339780434 R255B-P274B 0.516757395055354 F257D-L271D 0.516581917300616 R255D-P274D 0.514527850743693 F257C-L271C 0.514237490831709 F257B-L271B 0.511975067759476 L75D-F247D 0.509147969570208 L75E-F247E 0.508697448990432 L75A-F247A 0.507537377331632 L75B-F247B 0.506174864003697 L75C-F247C 0.504368542707366 Y236B-W287A 0.501642978158554 V81C-A243C 0.497493494545365 V235B-G286A 0.497200163022588 R255C-P274C 0.495954279722937 F80E-F80A 0.494337543035735 F80B-F80C 0.493917754259379 A243E-F283D 0.490092441533212 R255A-P274A 0.490021287956758 K30A-K289A 0.487638958566176 K30E-K289E 0.487292582790192 K30C-K289C 0.487252960108999 F80C-F80D 0.486867172241789 K30B-K289B 0.4867799643258 F80B-F80A 0.485856500232347 K30D-K289D 0.48495399952191 A243B-F283A 0.483388807058898 F276B-S246C 0.482002983615958 F80E-F80D 0.481672071495868 I28E-Q238A 0.478320522447719 V81A-A243A 0.477084303707988 L65A-I253A 0.477046019804079 V81E-S79D 0.476047142900436 L65B-I253B 0.475063091393253 L65C-I253C 0.474735111141064 Q256B-Y68C 0.474564202647629 L65D-I253D 0.474224476532745 S246B-F276A 0.473273355393592 F282B-V242C 0.471389040944846 V81E-A243E 0.470605393042648 S79C-V81D 0.470347719805393 S79E-V81A 0.469887380566353 F283C-A243D 0.468767173533771 S79B-V81C 0.467522363490096 L65E-I253E 0.466614592621818 V81B-S79A 0.465670802554434 Q238B-I28A 0.464645098999393 F283E-A243A 0.464319002169717 Q256E-Y68A 0.464123547245515 Q238E-I28D 0.464077295751702 Q256C-Y68D 0.463567881355731 Y68B-Q256A 0.462755325207261 Y68E-Q256D 0.460171592538847 F276C-S246D 0.459748544334263 I28C-Q238D 0.459525898857159 L82B-V240B 0.458789899271534 V81D-A243D 0.458394642090006 L82A-V240A 0.458302495583246 L82D-V240D 0.457860532613341 L82E-V240E 0.457824399108843 L82C-V240C 0.456936630060705 F282C-V242D 0.455870716016696 F276E-S246A 0.45298739421121 S246E-F276D 0.451164535385181 K30B-Q238C 0.450663717537874 V235B-W287A 0.450454253815419 K30C-Q238D 0.44916349369464 Q238E-K30D 0.449075284314997 F283B-A243C 0.449046376509969 L82E-W287E 0.448609122554569 K30E-Q238A 0.447252661663571 Q238B-K30A 0.447157976382426 L82C-W287C 0.442790314087492 V242B-F282A 0.442308577221739 L82A-W287A 0.439819505188142 L82B-W287B 0.439340750567378 L82D-W287D 0.436762069836148 F248A-F281A 0.435377917052012 F248E-F281E 0.431537674513085 W287E-V235A 0.431143765554843 F248D-F281D 0.430511101864421 F248C-F281C 0.429254701239861 F248B-F281B 0.428364399028362 I28B-Q238C 0.425875672199362 V235E-W287D 0.420860463781121 F282E-V242A 0.415350608861473 S79E-Y284E 0.407825144810835 S79C-Y284C 0.405984212498161 S79B-Y284B 0.404749052175168 S79D-Y284D 0.402573189642353 S79A-Y284A 0.401623143865898 L31D-K289D 0.399536465485813 Q238E-K289D 0.398986001503043 V242E-F282D 0.397210436686732 V81B-V240B 0.394387113753099 Q238B-K289A 0.392896735965297 K289B-Q238C 0.392510412930013 V244A-L288A 0.389742634369925 K289C-Q238D 0.388883281534557 I46A-I253A 0.38701941049717 V244D-L288D 0.386433118417528 V244D-A285D 0.38618334400146 V244B-A285B 0.386174722985852 V244C-A285C 0.385936544167923 V244E-A285E 0.385907226861194 V244A-A285A 0.385905953032855 I46B-I253B 0.385824680155902 I46C-I253C 0.385765125940536 K289E-Q238A 0.385442603247377 E35D-C251D 0.384580237855233 V244B-L288B 0.384289686855944 V244C-L288C 0.384270475993115 L31B-K289B 0.383328734360699 I46E-I253E 0.383065111693892 I46D-I253D 0.382613089548734 V78B-V244B 0.382542373097181 E35C-C251C 0.382298653971021 L42C-L252C 0.381980160025155 V244E-L288E 0.38098976246149 L31E-K289E 0.380866500753426 C69D-L249D 0.380097950362668 L31C-K289C 0.378099912522256 C69A-L249A 0.376919563753239 L31A-K289A 0.375466551463544 E35B-C251B 0.375012217942341 L42D-L252D 0.37485614181778 E35E-C251E 0.374291306208045 L42A-L252A 0.373186737270252 C69B-L249B 0.372629990867402 F36C-F281C 0.372584432978648 E35A-C251A 0.371695050967445 F36D-F281D 0.371219396995289 F36A-F281A 0.36818339087895 C69E-L249E 0.366532556769879 V81A-V240A 0.365878874728729 L42B-L252B 0.36414884929474 F36E-F281E 0.363564087194895 S79B-F80C 0.362417748036803 V78C-V244C 0.362403896090013 F36B-F281B 0.362147559770357 L42E-L252E 0.361530643531466 V78A-V244A 0.360198749176313 V78E-V244E 0.359448581097851 S47A-F257A 0.358182446123632 C69C-L249C 0.358056849008845 S47C-F257C 0.356898423265481 V78E-Y236E 0.3566223510167 V78B-Y236B 0.356567263790815 V78A-Y236A 0.356513505133619 T241B-L288B 0.354319253081262 K64C-C251C 0.353787697664229 V78D-V244D 0.353408962934749 K64C-Q256C 0.35328866545606 V81E-V240E 0.352894817904545 T241A-L288A 0.352079507361053 F257B-C69C 0.352073629602501 C69C-C251C 0.351267161584458 V242E-L279D 0.351013707094878 T241C-L288C 0.349045237247307 S47D-F257D 0.348780259496602 S47B-F257B 0.348766099925217 T241D-L288D 0.347636950588833 R255B-C69C 0.34658956886951 W287B-P77C 0.346439000173011 L279E-V242A 0.346001990403154 W287E-P77A 0.344423925042713 T241E-L288E 0.344193072649169 E74B-G254B 0.343677059056019 P77E-W287D 0.343649223096715 E74C-G254C 0.343079152503897 I28C-F282C 0.342818704396243 E74D-G254D 0.342113894482194 I28B-F282B 0.341078195607685 E74E-G254E 0.340913894134291 V81C-V240C 0.340626849518905 V81D-V240D 0.340224908539668 S47E-F257E 0.338476907981226 I28A-F282A 0.338334267393361 K64C-A250C 0.338216736105566 E74A-G254A 0.33804164704374 I28D-F282D 0.337604282358464 W287C-P77D 0.336720917871381 R255E-C69A 0.33643089433841 F257C-C69D 0.336190506479928 I28E-F282E 0.334492740973029 C69B-F257A 0.333721637959147 R255C-C69D 0.333336079918488 P77B-W287A 0.330139280559634 L279C-V242D 0.329258650285224 R255E-D270E 0.328489474727582 R255A-D270A 0.327661620952648 C69E-F257D 0.326670598795348 R255C-D270C 0.325866905727856 C69B-R255A 0.325530851948544 R255D-D270D 0.323679562401799 F257E-C69A 0.323553741458248 C69E-R255D 0.323464562838333 I76B-C251B 0.32305472671288 I76C-C251C 0.322652916580734 I76D-C251D 0.322504394683855 I76A-C251A 0.322071100577283 I76E-C251E 0.321885245604523 C69D-C251D 0.321444469268458 S79E-F80A 0.319908315756971 R255B-D270B 0.319782825261778 S34E-A285E 0.319709705036181 G83E-Y236E 0.319563013457852 V242B-L279A 0.319249808050036 S34A-A285A 0.318866924960318 Q280D-A285D 0.318783396309327 S34D-A285D 0.318753496970992 G83B-Y236B 0.318620521033965 Q280E-A285E 0.318090114778522 Q280A-A285A 0.318043613271356 G83B-F84C 0.318030115969771 G83A-Y236A 0.317979259490273 F84B-G83A 0.31793397473726 G83C-F84D 0.31792438474291 G83E-F84A 0.317899534763386 F84E-G83D 0.317668500758383 S34B-A285B 0.317192565417805 Y236E-I28D 0.317163464256514 Q280B-A285B 0.31707152987203 Q280C-A285C 0.315483941315975 L279B-V242C 0.315108271627498 S34C-A285C 0.314385841350026 F80B-S79A 0.313603338645489 Y236B-I28A 0.313414655963038 G83E-G286E 0.311156246556936 G83B-G286B 0.311146155442876 G83C-G286C 0.310994577033352 S79C-F80D 0.310846516968665 G83A-G286A 0.310637878546329 G83D-G286D 0.310438408107862 Y50E-R255E 0.309726064089395 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