[Wed Aug 24 17:27:52 2016] >Input file: TM4TQUATD.pdb >TM segments: M35-84_M92-121_M128-156_M181-205_M242-265_M290-315_N16-41_N76-102_N112-140_N142-161_N195-220_N261-283 MAIDEN score = -141.2912 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- P114M-S201M 10 C152N-W47M 1.9761044690893 P129N-P55M 1.68290590649314 I95N-I157N 1.38175252495901 M134N-L139N 1.34349737849112 L30N-I136M 1.22287736184566 I145M-E259M 1.21955670374889 I264N-I147M 1.19623955836411 C152N-T44M 1.13093262345064 I81N-W210N 1.0491302617182 H82M-D291M 1.0221378732125 I268N-I143M 1.01323696936626 I143M-V148M 0.976459336614142 V137M-F199M 0.908187266786489 L140N-L219N 0.902282619083971 I128N-Y290M 0.901774000970777 V271N-F142M 0.874336646034606 F37N-V265N 0.81548561353501 V88N-R209N 0.800911670766301 I145M-Y260M 0.785276378614969 W122N-L305M 0.77581372598427 T99M-L254M 0.770949779077153 Y204M-I242M 0.761923843271542 W122N-A304M 0.758910324009985 N211N-I145M 0.749032239976919 V146M-E259M 0.747127765463346 T90N-I148N 0.745210302438242 V146M-Y260M 0.743962076687501 Q134M-L139M 0.730616111359694 L140N-L220N 0.721755981286178 V88N-C205N 0.721185230390308 L100M-L254M 0.714117132294248 V247M-V308M 0.706479099902413 V148M-K195M 0.706266924176186 L250M-I307M 0.701427472683452 I143M-K195M 0.695100073698092 F123N-L305M 0.692324817201305 Y275N-L139M 0.689579211922708 L119M-L205M 0.678160327063361 N133N-K54M 0.676992316599891 A125N-F130N 0.675062828432709 F83M-I292M 0.672862797456992 I117N-F48M 0.672275037651518 A125N-M56M 0.672268262395303 A149M-L263M 0.66928920993986 I207N-F142M 0.669204568563522 F83M-A295M 0.667787103520112 G23N-V133M 0.666127773611578 I115M-D200M 0.657234373077655 A29N-W194M 0.651354093194868 L250M-V308M 0.64358560709375 C152N-F48M 0.641595108105181 L139M-K195M 0.641229642086667 T84N-C205N 0.640302853178126 W47M-L52M 0.638479667436275 H141M-E196M 0.63579796501995 A118M-L205M 0.632634605048313 I95N-C152N 0.624040813863804 L107M-P112M 0.623226935116335 F123N-A301M 0.620169847475058 P32N-L151M 0.618745006472941 M160N-L198N 0.612259513782286 M134N-L140N 0.608790480626192 F123N-A304M 0.604837066978975 A29N-I136M 0.60418470494119 W122N-V308M 0.600703933265924 A149M-L264M 0.598701262650492 N211N-I143M 0.594063412103328 Y213N-V146M 0.591221099948878 A62M-Y290M 0.584974807932711 F37N-G261N 0.582311362744419 P129N-L59M 0.579002424602532 L254M-A304M 0.575743153236751 A99N-I158N 0.575421652468004 I36N-V265N 0.573014464374281 V137M-A197M 0.571626296937566 M113M-I193M 0.57128314689821 I146N-R209N 0.567199762878624 G251M-I307M 0.560189466026177 L132N-P55M 0.559475190840787 N192M-A197M 0.557784400255693 L102N-L41M 0.557568978810358 G110M-I189M 0.556698667868261 L250M-A311M 0.553258150250019 Y83N-L147N 0.550442381367949 F82N-G87N 0.547056659662575 F130N-P55M 0.545242304714879 Y83N-V88N 0.541607662023679 F94N-P43M 0.535341267990039 F214N-E259M 0.53288262619386 W215N-L220N 0.525812115764822 F37N-A262N 0.522005127362036 F131N-D136N 0.521826419861782 Y138M-A197M 0.519290642953638 F130N-L52M 0.5157159049029 V96N-R161N 0.514451645890333 F83M-A296M 0.514230292453178 F111M-D200M 0.511871363981677 F130N-F51M 0.51153062477529 L105M-R185M 0.509044461780637 R143N-L220N 0.508533053771344 L139N-F144N 0.505715599769099 I146N-A216N 0.500835898620353 F131N-L219N 0.500220944771388 P112M-E196M 0.496628300281232 I95N-N156N 0.495141501577743 C152N-I157N 0.492762481925003 F94N-A151N 0.492657581746214 S91N-C152N 0.490876036419234 A118N-F155N 0.490653487497026 I115M-Y204M 0.490262969799122 L254M-N300M 0.484461239893876 F63M-T294M 0.482531753094122 P97N-L41M 0.481069621330842 T80N-W210N 0.479639733547581 V247M-S315M 0.473874564499178 C28N-E196M 0.471456775546903 S40N-I263N 0.47100702782252 V152M-S191M 0.47077273094142 V148M-S191M 0.465273626822968 Y83N-I146N 0.463136028245021 V244M-S315M 0.462434669664563 A125N-L52M 0.460877901347378 F83M-D291M 0.454796659927953 I50M-P55M 0.448302134604934 A125N-Y53M 0.448262583545786 T84N-W210N 0.447039188226418 H252M-A304M 0.446963408531628 P276N-K131M 0.444100855737346 L102M-R185M 0.44355612236161 I121M-R130M 0.442854224232873 G87N-I148N 0.442344190964458 I95N-T44M 0.440796471488516 S106M-V256M 0.437653197152138 N124N-K54M 0.433406535369918 S40N-V265N 0.431937630130154 L22N-I121M 0.430799351003317 A118M-I202M 0.429575313950304 Y83N-W210N 0.429148822937425 N124N-L52M 0.428805720048782 S91N-I148N 0.428188507343606 T90N-L147N 0.42796728667729 L116M-S201M 0.427425837803769 G98N-I157N 0.426719548592581 A99N-R161N 0.425680787153642 I116N-F48M 0.422988299319137 A99N-M160N 0.422981676657991 F111M-L246M 0.421508800600998 C152N-R161N 0.418395165142464 A29N-P140M 0.417994085334485 F142M-K195M 0.417320328211972 F63M-I292M 0.415335474105293 M40M-I45M 0.41522260289763 F86N-L147N 0.413984341850133 Y100N-L37M 0.413475533983786 F63M-I78M 0.409864666310298 F113N-I45M 0.408801334138634 P32N-K195M 0.406399732284711 L22N-V133M 0.40460064826926 A99N-L41M 0.40300799756233 F144N-G149N 0.402791300655828 P114M-K195M 0.39987793982892 V85N-R209N 0.399373436804882 M134N-W47M 0.398698840176498 H82M-I292M 0.397430639249828 A26N-I136M 0.396590239585548 F48M-K54M 0.396181067249096 S106M-F111M 0.395549461428576 V88N-A151N 0.39194184487527 V85N-A206N 0.388458360145483 I117N-I45M 0.384922423553159 L147N-R209N 0.383791500811272 Q134M-Y204M 0.383034689239334 R130M-I136M 0.382208233662819 F48M-Y53M 0.380913837918159 M127N-T294M 0.38005862148365 M160N-A197N 0.374435264429022 L117M-A197M 0.37432620070846 L139M-F199M 0.3735977747593 G126N-T294M 0.36971355708473 P140M-K195M 0.368415502755971 Q134M-G198M 0.368390251984453 Y138M-G198M 0.365970650639126 A125N-A296M 0.365783420083695 F109M-I189M 0.363695929206355 P55M-Q60M 0.362792441089074 L30N-V137M 0.361376355306657 C152N-K54M 0.361345481605206 A95M-A296M 0.361227969110653 N124N-P55M 0.360114119760693 L59M-T294M 0.358973197601548 Q279N-T135M 0.357111177456532 V277N-Y138M 0.356134474883417 V96N-L198N 0.353651087230642 F123N-V302M 0.352826868208281 V153M-L264M 0.352482285792341 V85N-W210N 0.35155005757044 A99N-A197N 0.351503841090539 V274N-Y138M 0.349739455146681 S106M-P114M 0.349307214629444 M127N-G297M 0.348480760671358 Y138M-K195M 0.347416367032226 G251M-L305M 0.346262011092453 A101N-M40M 0.344330265690147 L159N-A197N 0.342676163593726 S40N-A262N 0.341858363328111 E255M-F299M 0.340864163652661 W210N-V271N 0.340108478411297 N124N-M56M 0.339495291902185 I207N-G212N 0.339165029954259 I145M-K195M 0.338372618279092 N153N-R209N 0.337816080655731 M134N-L219N 0.336992110396311 G87N-A151N 0.33634426314283 G149N-Y53M 0.336023394725572 G212N-Y260M 0.335549080758802 Y31N-V148M 0.334306771704461 V137M-W194M 0.333449832722317 S208N-H141M 0.332687332519995 A118M-K131M 0.332107068571638 L139N-I146N 0.325957559033903 F109M-E196M 0.323148834297276 I248M-V308M 0.322152784386168 L105M-I189M 0.320225718815513 N153N-R161N 0.320102587658924 T101M-E255M 0.318405439767227 S106M-H252M 0.314312654306258 T120N-L52M 0.314061176174048 T120N-T44M 0.314035228412599 G126N-I298M 0.312905049330383 S91N-A154N 0.311410324119795 M121N-A154N 0.311171607831121 L92N-T202N 0.310609874868926 M113M-A197M 0.310336418872917 A125N-A62M 0.309677739022503 L105M-G110M 0.309025435424398 I128N-T294M 0.30866893688111 F63M-F80M 0.307914002979212 Y275N-K280N 0.307281123147676 I117N-A154N 0.306389746404052 I95N-M160N 0.306182557828606 F123N-E255M 0.306148796708567 I115M-L205M 0.305940222527282 I41N-A262N 0.30587992348069 P270N-S144M 0.303285826159862 I128N-A293M 0.302940862768383 H141M-S191M 0.30250216884337 G104M-H252M 0.301855087076585 L22N-M120M 0.300405464919171 P114M-I202M 0.30007286817936 C205N-I143M 0.299632415559174 L92N-P97N 0.298261179189817 I187M-N192M 0.298233274408089 G104M-G257M 0.297920761180091 R135N-L219N 0.297415835877202 L250M-A304M 0.297398129133581 L92N-A151N 0.296351487874523 T84N-P270N 0.296130422465166 G212N-H141M 0.295606359308479 G149N-G212N 0.295087058574054 N133N-F51M 0.293929028628635 L107M-R249M 0.292532111858705 V96N-I157N 0.291609975123713 G150M-Y265M 0.291000812074307 I93N-M40M 0.2901949151514 M120M-Q134M 0.290022101385726 D200M-R249M 0.289731049686248 Y35N-V148M 0.288658923328519 R185M-I261M 0.288588925511259 N211N-F142M 0.28816398271308 L204N-R209N 0.287731452478124 G98N-M40M 0.287481424318033 L59M-A295M 0.287444590659719 G110M-S201M 0.286764797551921 S76N-A216N 0.285558240149724 N124N-G58M 0.285189181046901 G110M-L246M 0.284296876455197 I93M-N98M 0.283794232517563 G150M-S191M 0.283317933320969 V146M-L263M 0.2828412978865 L119M-G198M 0.282763212311836 H141M-S201M 0.282640833459229 F113N-C152N 0.282494722478083 L102N-G114N 0.281792889732988 R209N-M217N 0.281405631437937 P97N-N156N 0.280968124600897 M39N-G261N 0.28071780887743 F63M-G79M 0.280651905143362 G98N-L36M 0.280543484713419 R161N-M40M 0.280333244620913 G149N-F51M 0.280018415724596 V244M-N312M 0.278813607052748 I95N-N153N 0.278643627295486 G23N-L117M 0.278509568651323 A125N-A301M 0.278198779987532 G126N-A304M 0.278126509949672 N98M-G257M 0.278028834227861 A26N-A197M 0.277897519739563 L119M-Y204M 0.277834587998675 Y281N-Q134M 0.277436776952261 G98N-N153N 0.276875351536832 C152N-T202N 0.276676479929507 T120N-A154N 0.275971179915094 L22N-G198M 0.275939184345753 G126N-A62M 0.275448033030409 P97N-M40M 0.274011870506221 A62M-A295M 0.273508158285603 G58M-D291M 0.272543538248171 Y31N-V133M 0.272384113126957 T80N-R209N 0.272225974097267 T84N-I207N 0.271574311287099 Y77N-S267N 0.271278312252038 Y35N-S40N 0.270472042975156 N133N-G58M 0.270064390468716 Y213N-I145M 0.26868681567238 F92M-K97M 0.268639365192099 Y100N-L41M 0.268060470839957 G251M-H313M 0.267405871734887 I146N-L219N 0.265569000070328 N79N-M217N 0.265447818971425 K54M-A62M 0.264802058662823 L105M-G257M 0.264063834932153 A118M-R130M 0.263884657409206 G110M-D200M 0.261131848641612 L204N-H141M 0.260015726765395 V96N-Y38M 0.259994904924154 N79N-G212N 0.259240626367265 P32N-S40N 0.259240274266904 I253M-A304M 0.258545599781492 F51M-G58M 0.257685921545992 A19N-K131M 0.256547686468855 N211N-P270N 0.256472830497193 L102M-V256M 0.25609445211128 P186M-Y265M 0.254988003323794 W47M-P55M 0.254049042340655 F130N-G297M 0.253969442063996 V244M-R249M 0.253926049553514 S103M-G251M 0.253925839564008 C28N-H141M 0.253918873387519 Q60M-P76M 0.253422898802042 Q134M-P140M 0.252279359747936 A29N-K195M 0.25225438278458 F131N-G145N 0.251397711450618 C205N-V278N 0.25065864024185 Q134M-E196M 0.250398946105591 N142N-L219N 0.249531992281164 M121N-R249M 0.249495634233227 M160N-I201N 0.249234485565169 Y83N-S91N 0.248624940278105 L107M-H252M 0.248285880647035 S103M-L254M 0.247380307504672 Y77N-V265N 0.245373516485293 A62M-D291M 0.245065227216259 P112M-L119M 0.24406476968149 V137M-E196M 0.243442437333949 P112M-L245M 0.241492513276196 L246M-N312M 0.240185956305456 P129N-M56M 0.240068304806329 N153N-S208N 0.239499542085246 A197M-Y204M 0.239399111290374 L245M-H252M 0.239319040450507 T101M-P186M 0.239279170355336 N133N-L52M 0.238914085990844 A62M-A296M 0.237984966337073 M113M-R249M 0.237947901468916 C28N-P140M 0.237558225328103 G110M-I193M 0.237256326326258 F130N-Q60M 0.237248534382411 M61M-D81M 0.236877514147768 V88N-T202N 0.236199693733299 H82M-G297M 0.235617059715127 P186M-Y260M 0.235576891295443 I272N-L139M 0.234780782424586 A99N-Y38M 0.233901512029305 K280N-K131M 0.233643755894203 G212N-F142M 0.233353347117256 D200M-V244M 0.233293510942682 V20N-I121M 0.233164431107836 I36N-V152M 0.2329589255089 F123N-A154N 0.231933128696971 M127N-R135N 0.23173846279612 P129N-L219N 0.231477739439927 G110M-L117M 0.231013217454102 T80N-L147N 0.229972484040042 A101N-R161N 0.229649271894311 F119N-A125N 0.229439391938301 P140M-S201M 0.229066386713314 A19N-M120M 0.228674391643578 I95N-Y100N 0.228508670093205 K131M-I136M 0.227991649380774 A149M-G257M 0.227941195489559 Y138M-D200M 0.226443572801852 P112M-L205M 0.226249991730191 G114N-N153N 0.225873390989224 R143N-M217N 0.225664177397638 T44M-L52M 0.225073574767535 V148M-I187M 0.224713979176292 H252M-G297M 0.223707732575328 I264N-V271N 0.223095086956758 V148M-I193M 0.222966080093609 L203N-P276N 0.222667855463178 F131N-Y53M 0.222538856499625 G212N-V218N 0.221377098010716 Y213N-E259M 0.220785977164354 H141M-E259M 0.220541444150394 Y100N-P43M 0.220535974793878 Y138M-F199M 0.220160699010925 S106M-I253M 0.219888706311531 N156N-P196N 0.21973076392286 I128N-G297M 0.219004739808604 K280N-Q134M 0.218699281379129 L102N-F113N 0.218545403074968 P114M-F199M 0.216140486765183 N98M-P181M 0.215588371788201 Y100N-M160N 0.215497444993257 P129N-A62M 0.215007162410245 I93N-C205N 0.214624014122021 Y35N-A262N 0.214508558209404 M121N-V302M 0.214239586314159 C152N-G212N 0.214108300432289 F94N-A39M 0.214100044468213 L246M-A311M 0.214063824436112 M56M-V302M 0.213629622074351 G87N-N211N 0.213554501847026 S91N-A99N 0.212794938461128 L59M-I298M 0.21250345530682 G23N-T135M 0.212207873352719 I307M-N312M 0.21207542393169 V152M-F184M 0.211813454295569 A243M-H313M 0.211334296255027 V146M-L264M 0.21115073606906 V148M-S190M 0.211097242050677 F130N-V218N 0.210649383145657 N211N-P140M 0.210363705534717 T120N-H252M 0.209711805228421 M134N-R143N 0.209424315613054 F184M-G257M 0.209210021897032 V89N-L147N 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