[Wed Aug 24 17:29:45 2016] >Input file: TM4TQUCGD.pdb >TM segments: M14-39_M57-85_M92-116_M146-168_M205-226_M257-279_N299-324_N357-382_N395-423_N425-443_N480-502_N544-565 MAIDEN score = -104.8395 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- P31M-P412N 1.71277620965367 P78M-S165M 1.43347615057951 I109M-E223M 1.26985533011288 M417N-L422N 1.12804617217579 I378N-I440N 1.10539796623658 T367N-C488N 1.0657616671215 Q98M-L103M 1.06457374741645 H105M-F163M 1.06269345373842 W23M-C435N 1.03812075513633 F106M-V554N 0.964033207448313 I85M-R94M 0.960544819445563 V101M-F163M 0.937511142013686 L214M-I271M 0.910976585902587 L269M-W405N 0.84766795709296 T63M-L218M 0.832384411100284 Y168M-T205M 0.817898504722558 I79M-D164M 0.809979208511218 I79M-Y168M 0.803814718817751 I109M-Y224M 0.769969328874891 N439N-I484N 0.75263876704192 S70M-I153M 0.746240802607359 L423N-L502N 0.743305379557155 V101M-E160M 0.724325427197376 V211M-N276M 0.71562553239481 V371N-T485N 0.714933353317988 V101M-K159M 0.711804147934573 A265M-W405N 0.710103846844947 A268M-W405N 0.708276240391706 T258M-I411N 0.707988725815238 I364N-W493N 0.679798571293368 L214M-A268M 0.677320559608514 Y366N-L430N 0.676962339954864 G215M-I271M 0.665538768685689 W158M-A312N 0.661205207560716 L18M-W23M 0.653904553788434 I109M-N494N 0.645140147574254 M404N-A437N 0.644573826612611 L71M-P76M 0.628715040641708 G428N-F433N 0.626246748178391 I100M-L313N 0.626115326673877 A82M-V167M 0.625399000726876 V101M-W158M 0.623403003388359 F365N-G370N 0.620337587953562 F427N-G432N 0.61923048646631 G370N-F433N 0.612869275401926 A82M-I166M 0.609771131199289 F39M-A259M 0.608313744360864 L103M-K159M 0.606942573317157 L66M-V220M 0.602385835094704 K30M-N416N 0.602240749508177 L375N-P380N 0.59284300525024 F320N-A545N 0.591328412443723 P31M-F413N 0.590911444753033 Y366N-V371N 0.585265543514927 A382N-I441N 0.582587350057886 F24M-Y29M 0.580729579323639 V110M-E223M 0.57292027448497 F377N-C435N 0.568821391642653 R213M-I271M 0.564211067625922 F320N-V548N 0.562497084728301 A113M-Y224M 0.558325557891024 I107M-K159M 0.554936163679523 I107M-N494N 0.554657206298367 L487N-R492N 0.552028587681993 K95M-I100M 0.54980549046877 L35M-A408N 0.545751742110966 I271M-N276M 0.545201324089403 I411N-N416N 0.545088145566931 I262M-N407N 0.543693542807575 P19M-F377N 0.539744037008583 I151M-N156M 0.537536996400202 I26M-N416N 0.536070382056382 V368N-A489N 0.530199591605196 F320N-G544N 0.529927865940992 V270M-A275M 0.529681422906046 T20M-F377N 0.527689755884876 I57M-N62M 0.527467973232326 V110M-Y224M 0.526601362915245 F27M-F413N 0.52590875052392 F377N-A434N 0.5104259782421 P31M-Q36M 0.50338791610026 F414N-D419N 0.499193172252018 Y102M-A161M 0.498872894404052 A265M-F406N 0.498496628664221 L214M-V272M 0.498006699909887 I400N-N436N 0.497284518424811 G215M-W405N 0.497131256892622 R94M-I100M 0.494361167943705 A408N-F413N 0.489524602807748 V272M-H277M 0.488965731696855 V101M-A309N 0.485191029669219 I107M-V112M 0.483863039588357 I317N-M322N 0.480196516039715 M32M-A408N 0.479791064194333 L28M-A408N 0.479719960820862 L442N-A480N 0.479016867070268 S67M-L218M 0.478429828700765 H216M-A268M 0.476802475214293 I378N-N439N 0.476356197421871 S67M-V220M 0.475196902272459 M84M-L305N 0.4747768134052 Q98M-Y564N 0.473967624083395 F75M-D164M 0.470064837458302 F369N-L430N 0.467006649726322 F27M-G432N 0.465315992896837 F396N-A401N 0.457913628132827 V101M-A161M 0.457761250456982 I26M-P31M 0.457630554686276 F39M-T258M 0.455918583376145 Q98M-K159M 0.454233943228912 M77M-A161M 0.446861746147575 K95M-K563N 0.440703340056043 M77M-I157M 0.440423042727496 I378N-M443N 0.439360954254997 G381N-I440N 0.438386783821564 F24M-C435N 0.436765297037946 G74M-I157M 0.431723817155866 V208M-R213M 0.431073202905908 G34M-F39M 0.430574717425214 F24M-I399N 0.430485717429031 I153M-W158M 0.430371476646368 T63M-V220M 0.42909653353992 L422N-F427N 0.427240132481638 K95M-P559N 0.425590252443041 M16M-I378N 0.424219875541277 V272M-W405N 0.423429567245399 L35M-P412N 0.423138556045807 L28M-F413N 0.422520855916375 L66M-R149M 0.421733866687859 L218M-N264M 0.417352731689964 N156M-A161M 0.415657141704847 L80M-S165M 0.414989001859904 L17M-P380N 0.414824287596955 R426N-L502N 0.413131767681777 T65M-E219M 0.411991022022979 S70M-F75M 0.41198944790925 V371N-C488N 0.410616078081428 I378N-N436N 0.407056817978802 I364N-F369N 0.406528643322762 Y318N-S323N 0.402998530067308 C488N-W493N 0.401642567581858 I490N-G495N 0.397936213276035 V371N-A489N 0.397342796105511 V211M-V272M 0.395888466150511 Y102M-F163M 0.395481194318842 F106M-K159M 0.395091811153441 I100M-A312N 0.394068797522297 P78M-I166M 0.391813317515346 I111M-I547N 0.391130817161691 I400N-A437N 0.390161867562559 L81M-S165M 0.388275660319176 V270M-W405N 0.387739487535474 V371N-A434N 0.387263625872851 S67M-I217M 0.386865321603118 F106M-I490N 0.386436573009093 T258M-G409N 0.386168406012308 F24M-K30M 0.385403341637618 Y558N-K563N 0.38534320868767 P31M-L415N 0.384509693408404 P31M-N407N 0.384132020448111 T63M-I217M 0.382553643868593 K30M-C435N 0.381206749838666 L35M-T258M 0.377932805486743 A15M-A382N 0.376828747317281 P78M-K159M 0.375364187839808 Q98M-G162M 0.37428151863612 A96M-A312N 0.373437564372933 V300N-L305N 0.371645963909751 I79M-S165M 0.369951215856492 M443N-A480N 0.368678244020516 L422N-L502N 0.368640868316821 Q98M-K563N 0.368325407087989 L69M-G74M 0.368209812661591 L71M-R213M 0.36693885605694 W23M-M417N 0.366299585320141 T403N-A437N 0.364926945934217 V112M-S155M 0.363275979501362 W493N-M549N 0.362788195811097 W357N-N362N 0.360441681626129 C435N-T485N 0.359359880952557 H216M-N407N 0.359084243799824 Y366N-W493N 0.358139107707051 L81M-D164M 0.357988723551411 L423N-G428N 0.357635542998208 V112M-Y496N 0.356983122486971 V97M-Y314N 0.356314751166844 S359N-I364N 0.354892068779808 A382N-A480N 0.352727625436809 A382N-I440N 0.352632884233108 M32M-P412N 0.351380890215507 G74M-I153M 0.349720273258798 Y93M-Q98M 0.349463751485884 Y102M-D164M 0.34721598473033 Y366N-I429N 0.346327619621679 S70M-P78M 0.34349995026992 P380N-N436N 0.342537720292754 F263M-A268M 0.342137267859078 L81M-A161M 0.341709491637185 V97M-Y102M 0.339457108565471 P104M-N156M 0.338941248291332 P76M-E160M 0.335448574920104 I411N-M417N 0.33389778985115 K30M-N407N 0.333392721175525 I212M-M404N 0.33168916904117 L81M-G162M 0.330043438450883 W23M-P31M 0.329399319695368 P104M-S154M 0.328300816801581 T20M-G381N 0.328274788360878 G34M-A257M 0.324465680579233 G215M-L269M 0.322716400870807 T363N-W493N 0.322229452722634 W23M-L28M 0.320957186092874 F413N-C435N 0.319910597486766 M322N-G544N 0.31953033355331 I429N-R492N 0.31923193588875 G68M-Y152M 0.317964136656757 T99M-C311N 0.317583439756324 P19M-N436N 0.316429929953508 I100M-A309N 0.316326524359724 M77M-V97M 0.315762621015803 S374N-A382N 0.314494614195292 P315N-S323N 0.312662663950589 V395N-M404N 0.311597987991576 N494N-P553N 0.31155115395922 F24M-I400N 0.308777481825883 Y102M-E160M 0.308037215043988 H105M-C488N 0.307280636610404 V112M-Y318N 0.307253789006927 F73M-I153M 0.304698506363359 K61M-L66M 0.303177980285991 F396N-C435N 0.300971417828219 Q98M-E160M 0.300959093802562 M417N-L423N 0.300081177199195 Y102M-P315N 0.299553085431356 M37M-D419N 0.299226475334604 L210M-A275M 0.298223890682468 S359N-G495N 0.297805248060061 L64M-L218M 0.29746784720231 M410N-D419N 0.29705070228427 H216M-G261M 0.297048731917393 T373N-L430N 0.297023440437619 E219M-F263M 0.296988821789551 L69M-R149M 0.296753810961398 G370N-R492N 0.296099580448788 V379N-I440N 0.295495958834458 S374N-C435N 0.295022869139132 G114M-S155M 0.293922558432266 I107M-C311N 0.2926903339214 L214M-E219M 0.292675085100681 G381N-C435N 0.292172506060037 I319N-I324N 0.29187185011027 A38M-G409N 0.291673264435865 L214M-A275M 0.289428220605882 M84M-Q98M 0.28896849255619 T20M-I378N 0.286673807718158 L304N-A309N 0.285125127884903 L83M-G162M 0.284143790700083 A382N-L442N 0.283443362783147 M16M-V372N 0.282957201886486 P78M-F163M 0.282716701160229 L66M-I217M 0.280942033582043 Y29M-G432N 0.280877171403446 R149M-S154M 0.280742792071221 L375N-A434N 0.280582818060701 N156M-C311N 0.280494678462228 I307N-A312N 0.279647715809564 L218M-A268M 0.278694927143247 E219M-G261M 0.278507674875682 P19M-Y29M 0.277457947092264 I319N-V548N 0.27610236243644 H105M-S165M 0.275150244678448 V97M-Y558N 0.2742677171085 L17M-A382N 0.273972671118528 G370N-G495N 0.273835059854513 M16M-P380N 0.272408351785541 S491N-A499N 0.271027601433575 H105M-D164M 0.27008786526906 F106M-N494N 0.269272753219174 A38M-A259M 0.269256931484749 L81M-G306N 0.269058811633839 R213M-E219M 0.268976843500376 M37M-N416N 0.268904817638954 S374N-V379N 0.26865864252365 G74M-H216M 0.268251462977308 G114M-Y318N 0.267984893299175 A113M-G221M 0.266696164230142 N436N-I441N 0.266193397894497 G397N-N436N 0.266027308625514 A82M-K95M 0.265624503473963 K30M-A38M 0.265276724718823 K95M-S165M 0.265261882443832 F27M-N416N 0.264368879465869 A82M-R94M 0.264251277277564 G74M-S165M 0.263737535560806 G370N-A434N 0.263591957824981 T258M-M410N 0.262301559047418 N264M-M404N 0.261009728645814 I60M-T65M 0.260614008062476 G162M-A309N 0.26049389518617 F106M-I551N 0.260311636504214 N494N-S550N 0.259644162941109 S67M-L72M 0.259580424904291 F433N-G495N 0.258651096275112 G74M-L210M 0.257790584276527 P76M-N156M 0.256633282972365 T363N-C488N 0.254499197002889 Y152M-I157M 0.25444180959704 I319N-I547N 0.254299879056072 L423N-G432N 0.253343822620827 F398N-C435N 0.252703746217604 T99M-E160M 0.252294463531848 P76M-L210M 0.250908258409194 T20M-T403N 0.250599456058841 F369N-G428N 0.250445833597209 I22M-F27M 0.249652046936058 Y366N-S374N 0.248358596538983 M417N-R426N 0.247861198386245 Y366N-C488N 0.24717801519451 P76M-L83M 0.245330203770719 V97M-L305N 0.24445760750478 T373N-I431N 0.244057273848063 S67M-G215M 0.242480571600455 L28M-N416N 0.241639981571858 H216M-G409N 0.241431099018937 L210M-N276M 0.240039194684977 L486N-S491N 0.239869952507949 P104M-W158M 0.238835131012341 P104M-C311N 0.238713620054848 R213M-L218M 0.238246805855805 S374N-A437N 0.23709319979921 R492N-M500N 0.236318275147147 F163M-Q562N 0.236029657942814 S67M-L214M 0.235830795300387 A161M-Y168M 0.234597276639339 G74M-L81M 0.233787372389749 V110M-L115M 0.232896893536067 F148M-G221M 0.232893457191781 V112M-I316N 0.232856265933112 V110M-I546N 0.232756279979929 P412N-L502N 0.232228706412753 K30M-F413N 0.231886498141778 V368N-I429N 0.231631269623515 A382N-M443N 0.231476746958005 V368N-R492N 0.230880464955893 A434N-A489N 0.230578625215751 T99M-L310N 0.230577955530247 V112M-I157M 0.230342435838743 M16M-G381N 0.230110707967311 S108M-P553N 0.228406344508802 V112M-I225M 0.227925320408291 I217M-V270M 0.227188588601227 S491N-S550N 0.22583664577369 N62M-L218M 0.225659533025232 I324N-V548N 0.225439441700672 F75M-A207M 0.224698541348948 L64M-I217M 0.224518305170625 Y224M-G495N 0.22406636460825 A96M-F308N 0.222282151060317 S155M-P315N 0.221876531739923 F433N-A489N 0.221603285095563 C435N-R492N 0.221541348156583 I151M-G221M 0.22029313275817 Y360N-V548N 0.219194642239652 L269M-T403N 0.218155798588179 E160M-C311N 0.218080556124637 Y366N-A499N 0.216716461572625 F27M-M32M 0.216611479717011 G381N-I400N 0.216564303007717 M410N-R418N 0.215280580668104 H105M-G495N 0.215070677009454 G397N-F438N 0.215026554804235 F106M-G162M 0.214982070141279 M84M-V167M 0.214404249527897 P104M-S550N 0.21439940998847 Y93M-F308N 0.21356369006613 M77M-D164M 0.213245949915527 T99M-P559N 0.213023530208524 P76M-L209M 0.212818469024309 A82M-D164M 0.212720057996054 H216M-T403N 0.212423744096595 I262M-V267M 0.212299358193956 F27M-G34M 0.212283145296666 V110M-F222M 0.211893218372424 S108M-V548N 0.211857400306976 I85M-V303N 0.211731984410855 W23M-I378N 0.210865875852589 I212M-V272M 0.210700692902705 V270M-F406N 0.210600922042926 S374N-A482N 0.210195741471348 V379N-L481N 0.210072682249634 F24M-G397N 0.209914896541123 Y366N-A434N 0.209213932462622 R426N-M500N 0.209016287962015 L64M-V220M 0.208788865877162 I111M-G495N 0.207449122077654 V116M-F148M 0.20722288793795 T99M-I307N 0.206650593459433 A312N-Y318N 0.206522052996822 L66M-E219M 0.206382243478815 M77M-A82M 0.206183290259158 M443N-I484N 0.205998969784034 I166M-T483N 0.205550239628086 L210M-H277M 0.205066820018667 L25M-T403N 0.204440606279967 G68M-G221M 0.204271919600672 H105M-A312N 0.203735757643281 I364N-G370N 0.203111729649635 T65M-H216M 0.202847480028733 G114M-I547N 0.202020905032585 T63M-G215M 0.201446388102623 I107M-G114M 0.201400718565174 L486N-P559N 0.201122488142455 L25M-N407N 0.200515583925529 I22M-T373N 0.200418569621497 L25M-N436N 0.200354164978751 E160M-N494N 0.199575421584053 G370N-I484N 0.199476932931423 R94M-N301N 0.199243214248553 R58M-T63M 0.198928586440116 I364N-T485N 0.198800343555268 V267M-V272M 0.198784330029007 G370N-N494N 0.198573373981157 H105M-L487N 0.19837186762105 C488N-Y496N 0.198150488778268 V110M-S155M 0.198137872200455 I157M-G162M 0.197634590915884 F427N-A499N 0.197372309974258 R213M-N276M 0.196742099362912 A480N-F565N 0.19597455730686 L28M-N407N 0.195434840813618 P150M-W158M 0.195240189746142 L269M-F406N 0.195198176623473 A59M-A260M 0.19501782198896 L66M-N156M 0.194990864548836 F414N-A499N 0.194428733863572 M443N-L487N 0.194227465194694 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