[Wed Aug 24 17:59:46 2016] >Input file: TM4U2QATD.pdb >TM segments: A524-542_A596-613_A795-812_C525-543_C795-812_B526-543_B595-613_B794-810_D526-542_D596-613_D796-812 MAIDEN score = -125.5729 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I525C-V530C 0.990935548677023 F608B-V795C 0.947825240751064 W526C-F531C 0.847218216873374 F608A-V795B 0.776720163800121 L803A-V601D 0.702532346266494 L799A-F608D 0.681666498962103 A532B-F796C 0.676489909574076 V800A-A532D 0.648474033476367 V604A-L799B 0.631054995101309 V604B-L799C 0.609580182257615 L540D-A598D 0.605684058018179 V601A-L803B 0.595764408350185 L799A-W605D 0.586190662892115 V604B-G802C 0.571869331868566 V536B-W605B 0.528211182252472 V539B-L803C 0.516072461674696 V543B-A598B 0.512077434624929 V543C-A810D 0.510971523366318 V536A-W605A 0.488430193052107 I600A-A806B 0.486268997103373 V601B-L803C 0.461931034634121 S597B-A810C 0.454388133822406 I600B-G802C 0.450508635549244 S597B-M807C 0.432002899997437 F608B-L799C 0.429453908316061 I600A-G802B 0.429448589467291 V543B-S597B 0.428038774930913 R599D-V604D 0.421496759513276 G802A-V601D 0.414041974753475 L540A-V601A 0.413505534999133 L540B-V601B 0.412103579721158 V543B-A810C 0.408718257535345 F608A-L799B 0.406361849172129 S597B-V809C 0.398858544985514 V601B-A806C 0.396762497730503 V539A-A598A 0.392167784385457 W526A-F531A 0.378975043581606 V601B-L799C 0.378743146215109 R599A-V604A 0.373333490995761 A806A-I600D 0.371760148718817 M807A-W605D 0.356207354253553 V539A-L803B 0.35558922299054 L799A-V604D 0.350605136946612 F608B-F796C 0.344412910795409 L542B-M807C 0.342705655694571 V543C-M807D 0.34114333278732 V539B-M807C 0.338236921469953 V539A-M807B 0.337267395730058 G801A-A532D 0.32903653602983 G804A-A532D 0.328585360025945 L540D-V601D 0.32710884728805 A810A-L596D 0.321068122770148 I611A-I613B 0.316847557666664 V809A-L596D 0.315417739928422 G801A-G535D 0.313698365839628 L803A-V604D 0.31266085773283 G794B-G802B 0.312593193341002 V536D-W605D 0.312271563290261 W605A-L799B 0.30977127862005 V795A-F608D 0.308072070347732 A806A-G535D 0.307821108971436 G535A-G801B 0.305122514911373 L610A-I613B 0.300106787974222 V536A-L803B 0.296181809331407 G535C-G801D 0.294240780956465 V536B-V601B 0.287837379968526 I600A-W605A 0.287788405966872 W605B-L799C 0.287548791814401 S597A-V809B 0.283852969029927 V539C-M807D 0.281509994239377 R599B-V604B 0.280757205455015 L540B-G804C 0.278794883992776 V536B-L803C 0.271718483984254 A532A-G802B 0.270481035726822 V536D-V601D 0.265119275914823 V530A-G535A 0.262011457556767 L803A-V536D 0.261989138047272 W605A-G801B 0.260142644956264 G603B-A806C 0.258155231736875 V543B-M807C 0.254374787249309 V601A-G802B 0.252275876222754 L542B-G804C 0.25161584648819 L808A-S597D 0.247190496151425 S597A-L803B 0.246634595924128 G603B-L803C 0.246224545282847 G801A-F608D 0.245684625037296 L540C-G804D 0.244868794261465 G802A-S597D 0.244741850563514 F607A-G802B 0.242392827531362 T609A-T609B 0.23760727365028 G801A-W605D 0.237209720170713 A528B-Y797C 0.235424238519012 R599A-A810B 0.235304412319339 F607B-Y797C 0.234990368559331 T609A-L610D 0.234096098123199 G804A-S597D 0.231932794829144 V530C-G535C 0.23143900134361 Y533A-A602A 0.230101950117438 V536D-I600D 0.226565019000886 V536A-V601A 0.22555225382474 T609B-L799C 0.225215642855703 I600D-W605D 0.224028934037612 Y533A-V604D 0.222718351334537 V536A-G603A 0.221373220209031 G603A-A806B 0.220313135027813 A810A-F541D 0.219192163181502 I600A-G804B 0.219151379816061 V536B-G603B 0.216833914764684 S537A-A806B 0.216341777115137 V601B-Y797C 0.214350719333374 R599B-M807C 0.214332969956274 V604A-Y533B 0.213396576704484 W605A-L610A 0.212917688422007 G801A-V601D 0.211402945296437 W605B-V795C 0.210274889711268 V809A-S597D 0.210010614555834 M807A-R599D 0.208545209203526 R599A-M807B 0.206459970390191 G603A-T609B 0.206365880858612 Y797A-A532D 0.204319248784496 V536B-F608B 0.204009165212861 I611A-G794B 0.203900904783735 W605B-G801C 0.203048689296763 I529B-G535B 0.201786617800742 I529C-G535C 0.20113022099571 A602B-A806C 0.200220396284768 F531A-V800B 0.200104803127648 I798D-G804D 0.199630982907205 V530C-V536C 0.199186415232464 V536B-I600B 0.199144266448664 V601A-I798B 0.198805373309921 V539A-R599A 0.194114741273798 G802A-T609D 0.192711611964989 S597A-M807B 0.192247311603742 G802A-W606D 0.1915293634491 I529D-G535D 0.188540858400029 V536A-R599A 0.187737521124291 W606A-G802B 0.185214454559271 V543C-L811D 0.185130603916531 L540B-G802C 0.184911862966464 G535D-L542D 0.183772937653858 L803D-V809D 0.183708579934946 G802A-Y533D 0.183111145904562 M527B-V795C 0.181294107113636 F531B-F796C 0.180464302720138 G603A-L803B 0.178961744629406 V795A-I529D 0.178960916870677 G603A-L805B 0.178870501761682 L542A-G804B 0.178668513028674 G535C-L799D 0.178413427777672 Y797A-F607D 0.174797991085575 T609A-T609D 0.173311395768824 V536A-S597A 0.170708815118183 F607A-Y797B 0.170606880396154 Y533B-A602B 0.17056179705388 W526A-T609A 0.169477027111614 I798A-I529D 0.167896605292376 G535B-G801C 0.166241823363384 L540D-G603D 0.166232358277106 V539D-V604D 0.164152796511821 G802A-W605D 0.163534347597744 V538B-A806C 0.163296193193773 Y533B-Y797C 0.162653912403269 F608A-F796B 0.162463903222793 V604A-V530B 0.162423418335561 L811A-S597D 0.162273274623065 G535A-Y797B 0.162161918520558 A602A-V800B 0.161984781463844 V604B-L803C 0.16075126090438 W606A-L610B 0.160523169015436 M807A-V539D 0.160361301371429 W526D-T609D 0.15831165266173 W605A-L805B 0.157841974109211 W606A-L803B 0.156488797081919 G802A-A532D 0.156214602716431 G535B-G804C 0.155000346617165 I529B-F796C 0.154255309904295 I600B-V809C 0.153489134679402 W605A-L803B 0.150559363331781 R599A-V809B 0.149450534817236 W606A-T609B 0.14926268161921 W526B-T609B 0.148209411669539 L540A-G802B 0.147763873467639 L540B-A598B 0.146550560776781 V538A-V800B 0.145515186709392 G535A-G603A 0.144391987998843 G535D-W605D 0.14352481604369 I534A-A602A 0.143309974142912 L610A-I798B 0.142780820172174 L799A-G535D 0.142290463303262 G603B-G801C 0.142279706752674 F608B-G802C 0.141984835897373 E524A-A532A 0.141535300606956 Y533C-Y797D 0.140830966421949 M527A-G535A 0.140633555718343 G535A-G804B 0.140588598175524 F531D-F608D 0.139646787946664 I529A-L803B 0.138449294626894 V536D-S597D 0.137226532385479 V543B-V800C 0.136299108993962 L803A-L596D 0.13599141662018 G804A-L540D 0.135642419909334 V538D-A602D 0.135469336795916 R599B-A810C 0.132695165449358 T609B-I798C 0.132604268200186 Y533C-L803D 0.132501377155493 I529D-I612D 0.132441314257751 G535B-M807C 0.131374150183296 F541A-R599A 0.131001914710847 A598A-A806B 0.130549298682389 V538C-A806D 0.130477247571537 A532A-Y797B 0.130358516025832 V530B-G535B 0.12994793400267 A528B-V800C 0.129523667081581 V601D-W606D 0.129255412776199 V539C-L805D 0.128939029563149 I611B-Y797C 0.128803378254334 S537C-V800D 0.128552227753285 L542A-L596A 0.128226701530064 G802A-I600D 0.127971476577688 S537B-V604B 0.127422915253114 V536A-V795B 0.127164133049986 Y533A-I612A 0.127117053908476 V539A-G802B 0.127103015865496 V539B-L808C 0.126791108491745 I812A-I600D 0.126714664167255 V536B-S597B 0.12475702214422 I798A-I611D 0.124748530024341 F796D-G804D 0.12468742068108 V539A-L805B 0.124334379717395 M807A-Y533D 0.124160063879164 G804D-I812D 0.123334819924441 I611A-Y797B 0.122251218507297 G801A-G603D 0.121146441515961 G535B-G603B 0.121103942544832 A602B-I798C 0.120372378153363 Y797A-A528D 0.120189116127993 I525A-G794B 0.120130566511095 A598B-W606B 0.119735805087239 F796A-W526D 0.119656116704419 L610A-W606B 0.118680359708053 Y533D-A602D 0.118517886262925 F608B-V800C 0.118315368969309 I534B-A602B 0.118247978359112 T609A-G603D 0.117480498161194 S537D-A598D 0.116970122275692 F607A-F796B 0.11635885234793 V539C-L808D 0.116310815494301 W605A-A806B 0.116236628095412 G802C-A810C 0.116217954175521 A528A-G794B 0.115929611695919 I798A-I600D 0.115092810137544 L540C-L805D 0.114744703354798 Y533D-I612D 0.114696391153492 A532C-L542C 0.114687334056675 G603A-V800B 0.114068642534198 V530A-V536A 0.114050942952188 V601B-W606B 0.113966153596764 V795A-A532D 0.113668609718454 V539A-I600A 0.11315503464772 L799A-L610D 0.113096744281865 G535A-F796B 0.112930092845192 L610B-L799C 0.112893160877957 V538B-G802C 0.112657368941266 L542B-L805C 0.1123736943395 M527B-Y797C 0.111837681369751 Y797D-M807D 0.111837681369751 L540A-L805B 0.111481968268026 S595B-V809C 0.111409604593939 V809A-V604D 0.111072912112169 L542A-L803B 0.111055946706088 F607B-G802C 0.110875232759646 G603A-V530B 0.110732508314405 L805A-L596D 0.110685814698028 V795A-L610D 0.110121576364794 V530B-F796C 0.109938585416256 V800A-G603D 0.109918925957467 G603B-V530C 0.10985493015905 V530A-G603D 0.109406853803811 G603A-G801B 0.108915418185541 M527C-Y797D 0.108863823161736 G802A-F608D 0.108784564916031 G535B-V601B 0.108412561803244 L540B-A810C 0.10815302959914 L808A-A602D 0.107903594401855 G603B-Y533C 0.107801655916967 S597B-W605B 0.107565957817724 L596A-L808B 0.10753936162806 I612B-V795C 0.10744572302281 L803A-L542D 0.107442786262663 M527A-I612A 0.107357694829478 A532A-G804B 0.106991572728281 L542D-L596D 0.106715305050287 F796A-W605D 0.106566409736732 G535A-A598A 0.106215073055726 V543B-G804C 0.106127010298142 V538A-A602A 0.106010599715397 I798A-G603D 0.105866927993191 S537A-A810B 0.105155258945275 W606A-W606B 0.105050876953612 V601A-L805B 0.104858308756395 L803A-A598D 0.104790683573905 V539A-V604A 0.104765563820612 L542B-L596B 0.104699751976313 A532A-W606A 0.104590316073824 F541B-R599B 0.104362834175024 V530A-V795B 0.104217465865128 L542C-L805D 0.103852053386645 L540C-V809D 0.103805975680179 G535D-A602D 0.103327719264102 L805A-L542D 0.102926989536319 F607A-G794B 0.102891242030955 S597B-V800C 0.102810412683366 V538B-A602B 0.102688455560097 L540C-L811D 0.102576082824561 L811A-V601D 0.102529930971145 V539A-L596A 0.102512381928591 L540A-A598A 0.102318808027632 G535B-G802C 0.102244116912661 V604A-G802B 0.102042837478692 G535A-W605A 0.101913206148383 A528A-V538A 0.101177242641423 V536C-V800D 0.101135040723568 G535A-V604A 0.100902735167732 V604B-V530C 0.100554888886802 V536C-L805D 0.100474457844346 V543B-G802C 0.100158307926878 I600A-A810B 0.0996202632323175 V538D-S597D 0.0987267097086311 Y533A-G603A 0.0986168638499922 A602A-F796B 0.0979392812586024 V604A-M807B 0.0976778800039133 I612A-I798B 0.0976005096602606 F796A-F531D 0.0975415307398584 V809A-G603D 0.0975343977324348 A528C-Y797D 0.0974786816230688 L540C-V800D 0.0973652949991282 I798A-V604D 0.0973243987197856 A528C-V800D 0.097226628267594 Y533B-L799C 0.0970974663935249 Y533D-L540D 0.0968194809545118 L799A-Y533D 0.0966997642899181 L540B-V809C 0.0963892562633801 L808A-V536D 0.0963413841665679 V604A-G794B 0.0961909929352287 V536A-Y797B 0.0961045968190044 V604A-V809B 0.0960861476062078 S597D-W605D 0.0960126895599527 G535D-V604D 0.0953336113734626 S537A-V604A 0.0953066560006648 S595B-G603B 0.0951615120532727 L540B-L811C 0.0950406912385175 A598A-W606A 0.0950137305962175 V538B-L799C 0.0949738079430782 G535C-L803D 0.0947259074895683 L805A-L540D 0.0945300774346791 A602A-L805B 0.0943754069731498 V539B-L805C 0.0943283747583705 F531B-V800C 0.0942663942112647 Y533D-F608D 0.0938496036447209 I798A-L808A 0.0938017842234385 I600D-L610D 0.0938017842234385 V538A-S597A 0.0929478879078096 L596A-I534B 0.0928385327625543 A532A-V601A 0.0926673654210354 L540A-V604A 0.0925241580315368 I798B-L808B 0.0923650592064506 V604A-L610A 0.092330620444427 V539A-G801B 0.0923141221268402 L596B-I534C 0.092223909235948 F796A-V604D 0.0922003392201045 I798C-L808C 0.0920053055912006 I534B-W605B 0.0919325057564748 I525A-L799B 0.0919091336682154 L596A-M807B 0.0917586287240424 V536C-A806D 0.0912675980193671 L799C-L808C 0.0909493417154295 V536A-I600A 0.0909429076626758 A532C-L799D 0.0905734774010627 V539B-S595B 0.0903738331770358 V543B-I812C 0.0901836650852895 I613A-V795B 0.0901547585352686 V536A-V800B 0.0900836242052385 L803A-V538D 0.0899507278104896 I529B-V800C 0.0898477577894665 I529A-V795B 0.0897554531035567 I600B-A806C 0.0894662701766666 I525A-L610A 0.0894519557709297 V538B-V604B 0.0893079579030189 L808A-L540D 0.0892634509776729 S597A-W605A 0.0892444462570781 L540D-L596D 0.0890470025409436 F531B-T609B 0.0889321854183194 L540A-L808B 0.0888675722165118 Y797A-T609D 0.0885663019562334 L540B-L808C 0.0884548051127592 L610A-I613D 0.0882297546183759 I600B-L610B 0.08816507231097 G603B-G804C 0.0875956295470481 M807A-G603D 0.0875255497759522 M807A-L596D 0.0873587467066854 A532C-G801D 0.0873468592246538 V536C-A810D 0.0871694615847831 F541A-A810B 0.0870233083607923 I529A-V800B 0.0869641492400577 F531A-T609A 0.0869289200062122 I612A-L610B 0.0864720621402885 V601A-W606A 0.0864511486256312 V800A-Y533D 0.0862951526064842 V539B-G603B 0.08559193108243 V538B-V543B 0.0855752489008754 I600A-L610A 0.0851877727692254 M527C-G535C 0.084777173179555 G603A-V795B 0.0847484003150983 I529A-S537A 0.0847014823779029 V536B-L805C 0.084393109132916 L596A-V601A 0.0842612667210823 I613A-I611B 0.0842597078913996 A532B-A602B 0.0840142123291283 L542C-G804D 0.0839706676073857 G535B-V543B 0.083707598224775 G804A-W605D 0.0836514028158506 V539B-L799C 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