[Wed Aug 24 18:18:22 2016] >Input file: TM4U4WATD.pdb >TM segments: A40-60_A75-96_A98-120_A135-151_A171-194_A213-232_A256-282_A288-305_A322-339_A346-367_A405-425_A435-455 MAIDEN score = -103.0410 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- W52A-C140A 10 P81A-G141A 4.48470644459502 C50A-V178A 2.50353689813827 C116A-W219A 2.42371621828447 P81A-L138A 1.93630578174583 R305A-L364A 1.86348138987293 S260A-I410A 1.42080709534789 C116A-W217A 1.30151907159701 W257A-I410A 1.25430526048224 I113A-W219A 1.24308497448364 Q182A-F295A 1.22506916128248 L264A-I413A 1.21762300823691 I300A-L360A 1.19875766978904 M76A-K423A 1.18474350218891 C116A-S216A 1.1724637258477 L54A-Q182A 1.09499034498107 C44A-T105A 1.08377215249861 V96A-W104A 1.05620300012492 W52A-F142A 1.02242662531152 S179A-F295A 1.00391633542942 C50A-W219A 1.00378748915217 V117A-W217A 1.00133827301947 F99A-W104A 0.993832112011903 A275A-P422A 0.981281889855537 F331A-V354A 0.974290306510871 V83A-I416A 0.966873731760018 V180A-F295A 0.96578188549423 L264A-G414A 0.931807242392777 V83A-F419A 0.920733257836226 F336A-V436A 0.915350067133077 V178A-F299A 0.886933304920205 Y263A-I410A 0.869592566762653 I326A-V450A 0.856887444547027 T266A-A362A 0.83903202816975 I113A-L223A 0.830032551382003 W118A-I136A 0.823358242365802 L119A-I136A 0.813257302995975 A106A-T227A 0.80285699728109 F107A-T227A 0.796444281400651 C44A-T227A 0.793418506010015 F267A-S366A 0.782482113948112 L80A-F419A 0.762826273613054 T338A-F348A 0.757891616323103 A79A-F419A 0.753754182240524 F270A-F359A 0.752213014454833 V178A-F295A 0.748580333256843 T78A-F419A 0.735580997779672 C44A-L223A 0.73334365291656 L264A-I416A 0.727317395328749 L80A-L138A 0.721455627643591 L183A-Q292A 0.720127197806484 L322A-V450A 0.719675366130573 I113A-V220A 0.714873961727214 F75A-M279A 0.710759667926928 L54A-C140A 0.701634689672757 F55A-L77A 0.697350271541572 F331A-L358A 0.695955324843999 V60A-M279A 0.68508507076506 L256A-I410A 0.682210812597973 F49A-G177A 0.680951015734964 T105A-T227A 0.679190170041516 V51A-C140A 0.678621560193292 F49A-A143A 0.676679177752474 I271A-G417A 0.675342586677323 F331A-A357A 0.675299663430755 L46A-L173A 0.675264776300875 L119A-A213A 0.674727013945886 I300A-F359A 0.668397543043492 V60A-K282A 0.667940718163133 F273A-I421A 0.662884681514601 A59A-L77A 0.66125440427594 K282A-V288A 0.656474505575231 L256A-A406A 0.655505100190165 A57A-L291A 0.650353112642535 V90A-F409A 0.649725752777628 F49A-V178A 0.649183969072417 L47A-L223A 0.645176415464762 F273A-G418A 0.62506961677323 L335A-F351A 0.623639621407071 F325A-V450A 0.621153399153316 G272A-F420A 0.619420204962918 F267A-A362A 0.617976229655866 F147A-G171A 0.614647481290385 F267A-G365A 0.613824523748375 S41A-S150A 0.613574382977808 S179A-F299A 0.612553969517882 G297A-F355A 0.612283522988377 L262A-C449A 0.611221484491677 I326A-Y446A 0.610382586033373 A59A-F75A 0.606368159335289 R89A-S411A 0.604440336893238 A106A-F226A 0.602784678186519 P186A-A215A 0.602129338674974 F270A-G301A 0.601334210760516 S269A-I421A 0.595239147890895 P298A-F359A 0.594026928486175 R89A-N146A 0.591970201535032 S332A-L358A 0.583932864594702 R89A-G144A 0.578241802547483 I40A-A229A 0.57443710444571 Q182A-L291A 0.574183098595181 M328A-L358A 0.571465878703854 L54A-S216A 0.57036256792513 V51A-W219A 0.567221387452942 L335A-V354A 0.551844205311459 F296A-L356A 0.550218864514434 F425A-P435A 0.541125039610428 F267A-A415A 0.537437683100969 S260A-G414A 0.53643160457615 F75A-P422A 0.536166968195 M176A-F299A 0.532819923116115 L47A-F222A 0.532140965672749 M53A-T78A 0.527585141527489 M328A-A362A 0.526920214399798 V58A-A215A 0.526390179998376 I40A-F226A 0.525979474996713 F55A-A275A 0.525901900487635 L335A-F355A 0.523570205186225 L183A-F295A 0.522820313234612 F94A-A405A 0.521564492665619 Y263A-I413A 0.521391077805553 P115A-I136A 0.520857780317616 S332A-F442A 0.517678705107549 L46A-M176A 0.517639872111754 R305A-L360A 0.516409313610676 F55A-S137A 0.516064133617421 L264A-A415A 0.515215149467817 F277A-A294A 0.512415623889404 L112A-F142A 0.511330790930178 L54A-M181A 0.510383384511807 F273A-F359A 0.509167999404999 T109A-A143A 0.505644183145346 P186A-L291A 0.505168673877193 L119A-W217A 0.504731609114504 G301A-F359A 0.503515670647331 A79A-F420A 0.500950447043498 V60A-A278A 0.498636776542856 S56A-G276A 0.498390929970873 F94A-S149A 0.498316864570604 I98A-R103A 0.498064016787904 I40A-R102A 0.496961112136368 M95A-W104A 0.496413958690137 S84A-L139A 0.494418394748384 V96A-R103A 0.492376984728704 R89A-F409A 0.488320266645277 V90A-A148A 0.485821589212367 R89A-A145A 0.48539682882762 F270A-P298A 0.481366185157313 V42A-L173A 0.481009954838878 L280A-F425A 0.479129506753928 A148A-L407A 0.476558139777824 A106A-I228A 0.475313400943483 Y92A-N146A 0.474250769786968 M53A-C140A 0.465850841088407 T109A-L223A 0.462467455756066 L47A-W219A 0.462162717721081 T109A-T227A 0.462029140571571 R89A-F147A 0.45588340187342 V83A-G141A 0.45347241417009 A185A-N214A 0.45107796680507 I300A-G363A 0.450436720147232 P43A-G174A 0.450294213558701 V96A-R102A 0.44896931023159 M176A-L303A 0.448846375498455 L334A-V354A 0.448181086830557 I193A-N214A 0.447160105756506 A265A-Y446A 0.445107528470946 S269A-G418A 0.44387275752496 A148A-S411A 0.440245567760574 A304A-T361A 0.437677360490382 K423A-P435A 0.433347832692539 W52A-A143A 0.43316458610496 L112A-C140A 0.432344740135099 L339A-F351A 0.429540486064743 C50A-M181A 0.429366479695813 V184A-A215A 0.427780339784836 K282A-Q292A 0.427167171574405 V178A-S274A 0.424256566268939 F273A-P422A 0.422801981396433 L45A-F147A 0.422385559751872 F75A-F425A 0.42040615257239 R89A-C140A 0.420007592939021 F194A-N214A 0.418413569964783 R89A-L407A 0.416487971132145 V189A-A213A 0.415311316404309 L173A-A302A 0.415139121799055 F325A-I447A 0.415111118292502 P81A-L139A 0.41473690881667 L327A-T361A 0.413359938698926 S82A-F419A 0.412062138379542 P115A-C140A 0.411497868348867 A304A-L364A 0.411193578030405 A86A-A145A 0.411081787604166 L46A-V178A 0.410150577786404 R89A-S149A 0.408731460615381 L261A-I413A 0.407832749117041 C50A-V180A 0.40772481692352 S56A-M279A 0.403433253097275 S82A-G144A 0.40256011059236 G301A-T361A 0.399217436444941 P81A-C140A 0.398828692640921 L88A-F142A 0.397722934979456 S56A-A278A 0.39132090160219 C449A-W454A 0.389752146481721 V83A-A415A 0.387534112872474 V117A-W219A 0.386783666343218 V90A-A405A 0.38645955242228 F267A-G417A 0.385885522663791 T105A-S150A 0.384371257924178 F226A-W231A 0.384037772163959 S260A-S411A 0.380307260479945 A59A-T78A 0.378542021073507 L280A-P435A 0.377584495198079 A275A-A415A 0.377479834614556 I40A-A230A 0.375583069647112 A57A-P186A 0.375509620809005 L264A-S269A 0.37459986296748 W52A-V178A 0.373821644143647 A79A-A86A 0.371596910827373 A329A-I447A 0.371286666091193 L87A-A412A 0.37120390679071 S332A-V354A 0.369849926080705 P43A-F226A 0.369390020671247 T78A-S137A 0.368958013002621 M176A-P298A 0.367161074337939 C116A-V220A 0.365914710326514 I421A-M439A 0.365600437315369 I326A-I447A 0.365502215419998 A48A-A148A 0.364594246828326 S56A-S274A 0.364590881474767 M53A-A275A 0.363826166429522 G301A-L364A 0.362506086456715 F194A-W217A 0.362430507551524 N324A-L364A 0.362349235669646 P298A-S366A 0.360734870236567 Q182A-P298A 0.36012755240857 K440A-C449A 0.360037130245189 C50A-G141A 0.359853460609186 S332A-P435A 0.357101696238307 V51A-S216A 0.356647731760461 A278A-L291A 0.35630661854767 N214A-W219A 0.355480977546723 L112A-A143A 0.35543449643811 S260A-F409A 0.354686987190361 T266A-L364A 0.353098231782247 A48A-T109A 0.351728502020578 L264A-S411A 0.350481189507192 A265A-C449A 0.349612918970589 A275A-G418A 0.34858018366913 L80A-S137A 0.345467548263295 N175A-P298A 0.345389395846447 A275A-K282A 0.344633074941374 N324A-G365A 0.34437332391401 P81A-N146A 0.342993361399512 L54A-T78A 0.342857262659525 M328A-Y446A 0.342166857681948 G110A-C116A 0.342157521195115 L88A-A412A 0.341345881385301 I300A-L356A 0.339197056038689 F325A-Y446A 0.337716163551137 V42A-G171A 0.336805458620706 A278A-I290A 0.336585165581649 T266A-S366A 0.334845330267038 V90A-A145A 0.334694707647586 M151A-L407A 0.33422015358091 S274A-G297A 0.334118405141905 S41A-A230A 0.330467669656215 M176A-I300A 0.329478175918557 R102A-N146A 0.329403007522981 P115A-L139A 0.32934699641053 A148A-G414A 0.329319183134185 A185A-S216A 0.329059724355695 A86A-S411A 0.327827925618965 L322A-W454A 0.327253476909593 S332A-M439A 0.327041924296291 A59A-M279A 0.32607267101401 A362A-G417A 0.325981266965117 A86A-I416A 0.32538663383455 A79A-G268A 0.324918281464312 L112A-L223A 0.323641882774768 M53A-P81A 0.322678649544751 A48A-S149A 0.322281531077084 C140A-I218A 0.322181956810135 A185A-A294A 0.322013986827398 M95A-G100A 0.320780554046775 S108A-A143A 0.320311497040911 C44A-I218A 0.31989170291775 G100A-A230A 0.319626484554332 V117A-V220A 0.318428523069612 F49A-G144A 0.317966742878204 F331A-F355A 0.317943137069575 G272A-F419A 0.317863754657669 L119A-S216A 0.317385078611987 T109A-F226A 0.316740666555017 P91A-S108A 0.316502795655501 A48A-G171A 0.314818887929174 G172A-G365A 0.314623036879112 V178A-Y293A 0.313535209871661 A265A-S411A 0.312858064568584 S41A-T105A 0.311920525440959 W52A-G144A 0.311286943772513 L45A-S150A 0.310828628059056 M279A-V436A 0.310514309785448 M53A-N146A 0.310396136051918 K282A-I290A 0.309836121235323 G333A-A357A 0.309309101839078 A143A-G174A 0.308649591484683 A294A-A353A 0.308457191349768 K282A-S347A 0.306600960798145 A148A-G174A 0.30624886858077 P81A-F419A 0.306150195375991 G272A-A278A 0.306149212120833 Y92A-G408A 0.30557757245676 G174A-G367A 0.305447050063455 F49A-G174A 0.30489734575985 V188A-I218A 0.30317081646423 G276A-I421A 0.303101936508294 R103A-A230A 0.303040124808012 S192A-A215A 0.302751207111166 G85A-G272A 0.302236459148535 P81A-S274A 0.300540686612385 L407A-A412A 0.300469864164707 A106A-S149A 0.300391445633148 G171A-G301A 0.300179377285969 M151A-G171A 0.300113280576748 M95A-S149A 0.299141983927667 M53A-V178A 0.298713786399473 A86A-S93A 0.298125304700052 S260A-G417A 0.297676301973144 T266A-T453A 0.297133124616078 V90A-G408A 0.296440525844496 C50A-F226A 0.296173714464054 T266A-P298A 0.296046791842402 A329A-I444A 0.2960087252246 S274A-K423A 0.294162990599915 A48A-L112A 0.293653989741777 S93A-L407A 0.292927813666723 G417A-Y446A 0.291789330964781 S108A-A224A 0.291180823898499 F75A-G276A 0.291033821743529 S137A-G144A 0.290913397828043 S281A-V288A 0.290598370722034 L54A-G120A 0.289454627753675 L87A-A145A 0.289438103672476 G301A-A362A 0.289105607304133 R102A-M151A 0.288678129050047 W118A-W217A 0.28848088892601 R103A-N146A 0.288438627690327 L80A-G276A 0.288098544547782 A148A-A415A 0.287209818522488 G333A-G437A 0.286973628851088 A302A-L360A 0.286712496539575 S108A-P115A 0.286585790022827 C116A-I225A 0.286301537255518 G174A-G363A 0.285171846049218 F267A-G363A 0.28491291060825 G171A-S411A 0.2843372242577 S411A-G418A 0.284159000166115 V96A-S149A 0.284015752954045 K282A-Q289A 0.283243739189561 L77A-G418A 0.282880783456588 V51A-L112A 0.282362357383114 T338A-F351A 0.281742846241311 A48A-F147A 0.280888839555416 L54A-A215A 0.28018601835287 G144A-G272A 0.279219369749969 L183A-A294A 0.278454038740022 G177A-L303A 0.277417386464132 V180A-F299A 0.277087126985146 L262A-G417A 0.276716236378129 A329A-A362A 0.276060733819345 V83A-G418A 0.275649832348137 Q182A-M279A 0.27549979144085 G171A-L407A 0.275108672098626 F55A-T78A 0.273954602286597 G85A-A415A 0.272966541944001 L54A-G276A 0.272746500781812 G141A-A412A 0.271864515637493 A329A-A357A 0.271040476231645 G177A-S274A 0.269472073432081 A79A-A275A 0.268735604237624 P186A-N214A 0.267435740620186 S108A-G141A 0.2667396024484 L337A-G346A 0.266711384422196 S84A-F142A 0.265884889717846 V60A-Y293A 0.265824158217391 C50A-M176A 0.265555783651754 A145A-G414A 0.264769017497029 G177A-A185A 0.264561968547619 R103A-A229A 0.26398895330674 F267A-G414A 0.263956123537473 M328A-L443A 0.262966276489113 L303A-G363A 0.262523852477024 G333A-L339A 0.262140259086139 W52A-G174A 0.260717027373723 S281A-P422A 0.26022953360401 V117A-S216A 0.259897886757692 G144A-G268A 0.258969207184988 P81A-F142A 0.258603121806691 S269A-K423A 0.258391530096151 V96A-G101A 0.258308704725796 L261A-G414A 0.25827462262895 N175A-S366A 0.257738954983933 N175A-S274A 0.257470933918367 G272A-F442A 0.257455501323282 G346A-F352A 0.256811972086484 A57A-A185A 0.255310403237124 V83A-L138A 0.254856371924143 G110A-A224A 0.253555948909978 I271A-A415A 0.251660025537813 G120A-N214A 0.25164630517781 G174A-F299A 0.251086389790748 R305A-N324A 0.249973869002133 S332A-Y446A 0.249147609129116 Y263A-S411A 0.249082978839066 G177A-W219A 0.248746728959409 L173A-G301A 0.248634234075158 N175A-G297A 0.248613541911793 G363A-G414A 0.248417771214361 N324A-A362A 0.247926893796667 A275A-S281A 0.247673136194136 G100A-S150A 0.247362952228325 S190A-Q292A 0.246991476753972 G101A-N146A 0.244184155790319 A265A-A329A 0.243258855451475 P91A-S150A 0.243205168966516 S274A-G363A 0.243027988658419 C44A-V180A 0.242626535627646 I421A-F442A 0.242100494978072 Q289A-M349A 0.241260581780858 L335A-M439A 0.24111025826438 L322A-T453A 0.24056846746867 A185A-I218A 0.240358449862964 C140A-V220A 0.239767031523707 F49A-G172A 0.239113862837169 G367A-G414A 0.239110450231028 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