[Wed Aug 24 18:29:31 2016] >Input file: TM4U5CCGD.pdb >TM segments: A500-530_A535-560_A718-742_C1996-2026_C2031-2055_C2210-2233_B1250-1279_B1283-1308_B1463-1487_D2740-2770_D2779-2804_D2964-2988 MAIDEN score = -135.5044 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- V1997C-N2213C 1.55406922200038 L2964D-P2003C 1.38823427506667 I2035C-W2040C 1.01020293458725 L2964D-L2004C 0.987238457585989 F2744D-V2969D 0.969346775262224 L2211C-E1261B 0.861093408837066 L2004C-W2009C 0.827301303498284 V1470B-F548A 0.825208289268614 V2217C-F1296B 0.805128106213127 W2789D-L729A 0.803567679322102 L508A-W513A 0.800519728828201 V2972D-F2043C 0.798520460608727 L2966D-E2007C 0.788665506310602 L2964D-D2002C 0.781600119080198 F504A-V722A 0.777626155598826 F2792D-V725A 0.774076330549923 I2048C-S1302B 0.759517351250728 W545A-L550A 0.755985581257946 V501A-A723A 0.752500215233432 W2040C-L2045C 0.747678637852438 L2980D-V2036C 0.735683234846721 W2789D-L2794D 0.71828110101602 L2976D-W2040C 0.718266732058458 W2041C-I2046C 0.708725228641494 S1283B-I1288B 0.686934646150933 L1255B-Y1260B 0.683907242138656 S2210C-I1262B 0.678792212891032 L2004C-Y2051C 0.668820376121722 L2976D-V2039C 0.663993814145972 L527A-A538A 0.663193459919763 L2001C-Y2006C 0.657449598339242 L1474B-W545A 0.646867654968924 N2803D-A2053C 0.646187904043558 A1481B-V526A 0.644208447627314 L2221C-W1293B 0.640403560198365 L505A-Y510A 0.637920481228515 N2054C-A1306B 0.627155603808798 I540A-W545A 0.622850290788033 F502A-G724A 0.622542395699384 V2770D-A2782D 0.621258083554865 I2784D-G732A 0.613476985205416 F2744D-G2971D 0.611554298652591 W1293B-L1298B 0.608794822632058 L2748D-W2753D 0.591841678175406 L2211C-A1259B 0.591841276067242 R2034C-V2039C 0.580872704761418 I1473B-V544A 0.562880862394487 A1485B-V530A 0.56219798817849 I2220C-V1292B 0.559460899466391 V2770D-A736A 0.556774457711051 A2802D-N559A 0.552430435086027 S2781D-L735A 0.550409098497849 S2799D-L560A 0.549996679786172 E1261B-I1266B 0.549760378305324 G2979D-I2035C 0.544327078396854 G1477B-I540A 0.54386452642791 L2055C-S1303B 0.542192961243298 I2797D-S2049C 0.542005892195252 L508A-Y556A 0.539300683707918 L2023C-A2033C 0.53886908043822 I2784D-W2789D 0.535114082877932 P2747D-L2804D 0.531451681051877 L2966D-A2005C 0.530669694086376 V2785D-A736A 0.523988055911754 S1253B-S1465B 0.520299516312888 G1996C-V2214C 0.51940736402822 P1257B-L1308B 0.518386286287401 Y1260B-A1265B 0.506599943969473 Y2750D-A2755D 0.504587282267721 W513A-F518A 0.501060791926989 E2007C-I2012C 0.498337800159538 G2224C-V1289B 0.497913181814033 V2741D-A2970D 0.492019282356376 L2966D-I2008C 0.491507419547796 A1481B-V541A 0.491211963466139 L2221C-V1292B 0.488632705122069 L1474B-V544A 0.488174720131694 S503A-S720A 0.480658073735118 I2784D-A736A 0.48021887463761 S2965D-L2004C 0.47864843305083 L2980D-I2035C 0.47834795245189 G2224C-V1292B 0.474850780148831 V2770D-S2781D 0.474061578035099 V2741D-S2967D 0.473445842931836 G2740D-S2967D 0.472695166205146 A2228C-I1288B 0.47233255485599 L2748D-S718A 0.471132225184668 F2218C-G2223C 0.470858290040965 W2790D-I2795D 0.469649547128913 A2228C-V1289B 0.466688969459433 Y2006C-A2011C 0.464173362833307 V1251B-A1468B 0.464137452340308 R1287B-V1292B 0.462458443811653 I1301B-S554A 0.460620313248801 S2798D-Y556A 0.460104519079567 V2788D-L729A 0.459211450504815 Y2219C-G2224C 0.458164014010265 S1463B-I512A 0.457803049793909 R2783D-V2788D 0.456465551679359 G1250B-N1466B 0.455048871952908 S1274B-L1279B 0.453674766769487 R539A-V544A 0.452646004191142 V2785D-L733A 0.452256064486859 V541A-W546A 0.450780844872164 W1263B-F1268B 0.44938039382173 I1288B-W1293B 0.448024506704726 F504A-G724A 0.444998104837189 V2788D-G732A 0.444720505209004 V2214C-Y2219C 0.444269766316586 P2003C-L2055C 0.44412458324997 V2766D-A736A 0.443138928716014 M2229C-S1285B 0.43765991297384 Y2800D-S2049C 0.43678659444693 S2798D-I553A 0.43647942374162 P507A-L560A 0.436463339206138 L2804D-S2050C 0.433691789002678 V1467B-Y1472B 0.432003636950843 W1294B-I1299B 0.428424853999609 A2983D-V2036C 0.424475426157266 W546A-I551A 0.422967558660631 V2036C-W2041C 0.413680664536884 L2780D-V739A 0.410524708924793 L2980D-V2019C 0.402041868740763 V722A-Y727A 0.401279368566012 F1998C-N2213C 0.400174604623715 V501A-V725A 0.399415019931589 S2799D-T557A 0.395947022063625 A2983D-V2022C 0.393190194445395 V530A-A538A 0.39305469420534 S2781D-A740A 0.392569276848748 G2979D-V2039C 0.39020763181679 L2980D-V2022C 0.384437532911838 T2052C-S1303B 0.384235865505925 V730A-L735A 0.383850752972117 G1477B-V544A 0.382243638653885 W2009C-F2014C 0.381317858993146 V530A-S537A 0.380684464048033 S2050C-L2055C 0.379464233689818 L1308B-S555A 0.377827098766011 V2770D-L741A 0.377364277173884 V2785D-W2790D 0.377215493387499 V2763D-W2789D 0.372899332332207 L2767D-A2782D 0.371707110921822 S2798D-L560A 0.370718188625204 V1273B-W1293B 0.370028065673268 V2021C-V2026C 0.369130432091525 E2751D-I2756D 0.369045732867164 L1478B-V526A 0.367930033376052 V2757D-L2762D 0.365504480250529 L2745D-Y2750D 0.365267048983039 V2766D-L733A 0.362298697291584 M2984D-V2022C 0.362249274647812 V501A-G724A 0.361659039055691 V2763D-V2785D 0.359813821185826 S2799D-P507A 0.359477356278684 V2972D-Y2016C 0.356810548630564 Y2219C-V1273B 0.356386814596663 A2987D-L2025C 0.355317523459963 Y2016C-G2038C 0.354029560876725 V2770D-M737A 0.353941978230224 V2788D-I728A 0.353607799186895 V523A-W545A 0.352568721408749 V2013C-L2018C 0.349180524822811 L1478B-V523A 0.348662951283321 G1996C-A2215C 0.346675711210442 A2970D-A2005C 0.346646546051786 T2801D-S2050C 0.346190358791316 D2002C-Y2051C 0.344396411521566 F2000C-N2213C 0.340715670003312 A2755D-A723A 0.338605747550282 I1299B-Y1304B 0.338188333655955 V1289B-W1294B 0.338174987470681 F1295B-I1300B 0.337332121143479 V1470B-Y520A 0.334430221390108 P2003C-Y2051C 0.333690650889595 L1258B-W1263B 0.33296560786248 V1251B-Y1260B 0.33152346274752 G2978D-A2015C 0.330486456804171 G2226C-A1286B 0.329760240780217 A2983D-I2035C 0.328128379907547 Y1304B-S554A 0.328056840904313 S2798D-P507A 0.327779414747583 G2979D-A2987D 0.327710691364888 L2769D-M737A 0.327569795383115 V517A-L522A 0.32747883181271 Y2219C-G1291B 0.326835352321433 A1481B-G543A 0.326776287811041 G1479B-A538A 0.326190794710348 G2979D-V2036C 0.326169044854413 G1477B-A519A 0.325057090506314 V2741D-V2969D 0.323985230369599 V1467B-Y556A 0.322443960227339 A2782D-G734A 0.322106731169422 S2967D-A2011C 0.319498734019132 G2979D-Y2016C 0.317696222069147 V501A-S720A 0.317406348436357 A1485B-S537A 0.317355749837083 E2751D-F726A 0.316063210362641 V1997C-V2214C 0.315368189590578 P2747D-Y2800D 0.314840074482793 Y2974D-V2019C 0.314383588942512 V2231C-L1284B 0.313751387799631 V2222C-M1264B 0.311425478826671 Y1270B-V1275B 0.310131785347766 L2055C-N1307B 0.309942303493503 N1307B-A558A 0.30888907910486 V2019C-V2036C 0.308164622517157 A2802D-S718A 0.30758175321916 S1463B-L508A 0.306941320212065 L2964D-E2007C 0.306217648401554 L2211C-I1262B 0.305028908845356 A2749D-S2799D 0.303254920004695 V2972D-V2977D 0.303165666524658 A2053C-A1306B 0.300963627703522 V501A-V722A 0.300261066180638 L2769D-A740A 0.299962620558464 L2227C-A2232C 0.299786542967883 L1480B-A1485B 0.299239764079754 L2804D-N2054C 0.297548759743693 A2215C-G2223C 0.297077368041322 G2981D-G2038C 0.296088491482255 V1267B-L1272B 0.295727024511006 I2048C-A2053C 0.295059154838523 L2225C-V1289B 0.294813907383336 M514A-A519A 0.294776022995113 S1303B-L1308B 0.294311184866499 Y2051C-S1303B 0.293372232898948 P2003C-S1302B 0.292297934795447 I2975D-V2039C 0.292121729822386 V2977D-L2982D 0.29011696303192 S1283B-G1291B 0.289691320740357 G2223C-L2230C 0.288582504120618 G2226C-L1272B 0.288171391493132 F1252B-N1466B 0.287191269306607 W2009C-T2044C 0.286077848055485 L735A-A740A 0.285624774665493 L1464B-A509A 0.285164680503704 G2224C-L1272B 0.284856038681823 T2044C-W1294B 0.2847771395593 T1305B-S555A 0.284771976103536 V2972D-V2039C 0.284553370678433 Y2974D-G2038C 0.283009832751656 V1251B-V1467B 0.282995832518802 S2799D-L2804D 0.281565713074702 V2222C-V1292B 0.281492603237142 V1251B-V1470B 0.281330329544398 Y2016C-V2021C 0.281243618171811 A2987D-L2031C 0.281088167040878 L1277B-A1286B 0.280895192349019 A2983D-S2020C 0.280355745700367 L2780D-G732A 0.279918510291816 L2982D-A2987D 0.279090190939748 A2232C-L1284B 0.278592378941623 S2210C-A1306B 0.277234848434015 G2787D-G734A 0.276912991097889 G2979D-A2015C 0.276892391486108 A1481B-A538A 0.275984834015711 V2986D-L2031C 0.274100995993204 L1298B-S1303B 0.270797541224156 T1297B-W546A 0.270551392933864 I728A-L733A 0.270077617825097 V2217C-Y1270B 0.268305070308011 P1257B-N1307B 0.267741886462465 L2964D-A2005C 0.266590211290427 G1479B-L522A 0.265669091196551 F502A-N721A 0.265582944850037 S2965D-N2054C 0.263322267707103 P2003C-S1303B 0.26325772405051 Y1472B-V523A 0.262481580992416 Y1472B-G543A 0.262012239355649 N1466B-S555A 0.261836415274529 G2216C-A1265B 0.261261212768283 G1477B-W546A 0.26110852382472 T2793D-W2041C 0.260147944470898 W513A-T549A 0.259843645180765 L2780D-A740A 0.258752354794405 A2759D-G732A 0.25856276109857 W2753D-T2793D 0.257035397062084 V501A-Y510A 0.255569362039637 S1999C-L2055C 0.254599652209001 V2741D-V2972D 0.254246188360071 N2968D-S2050C 0.25418357944455 L1478B-V541A 0.253997548642835 G2979D-F2042C 0.253653357291021 G2978D-W2040C 0.253148241641852 Y2760D-V725A 0.253106164890356 S1999C-N2054C 0.253064917956079 W2790D-G732A 0.253045242795117 L2762D-G734A 0.252890615757577 A2983D-L2988D 0.251887128949968 G1477B-F547A 0.251262037448803 S1463B-A515A 0.250961665291348 G2979D-W2041C 0.250744994178716 P1257B-W1263B 0.249973213713893 W1263B-T1297B 0.249883123852372 V523A-V541A 0.248062112651082 G2787D-Y727A 0.248038561046434 V526A-V541A 0.247719565400629 M2984D-R2034C 0.247281474308941 L2966D-P2003C 0.247237848830349 R2783D-A740A 0.247232555279443 M2984D-L2025C 0.246523875820934 G1477B-Y520A 0.246390886778135 I2795D-Y2800D 0.246320454057735 V1251B-D1256B 0.246266214912199 T2044C-T1297B 0.245667621296349 A2802D-A2053C 0.245251896032858 S2210C-L1258B 0.245051767903797 V525A-V530A 0.244933971242283 N1307B-S555A 0.244848761249322 V2788D-V730A 0.244684190455392 G2971D-A2011C 0.244677520001956 S2965D-A2011C 0.244464171289522 S2210C-G2216C 0.243247468589892 F2791D-G732A 0.242916174937106 G2223C-W1293B 0.242425614362116 S2965D-A2970D 0.242307342064224 L2964D-T2052C 0.241771635365925 T2793D-T557A 0.241619185550912 T2793D-L729A 0.24110064915918 A2749D-S720A 0.240285231087403 A2802D-Y2051C 0.240158023943505 G2978D-L2018C 0.239176325178361 T1305B-T557A 0.239094336974079 P2747D-T2801D 0.238328074296842 L2794D-T549A 0.238038736143432 W2789D-G731A 0.237425347486548 T2793D-T549A 0.23724418197286 T1297B-T549A 0.237071074152674 S2210C-A1265B 0.236498853781237 L1258B-T1305B 0.235756670746421 R2783D-M737A 0.234951847584703 S503A-Y510A 0.234950335421755 G2971D-F2042C 0.234824454604337 A2755D-F2792D 0.234818129513989 A1481B-S524A 0.234374654906931 L2748D-Y2800D 0.23403218619859 T2044C-L1298B 0.233943613433581 L1284B-G1291B 0.233897215333545 Y2016C-F2042C 0.233690470260978 S2965D-I2008C 0.233315386447584 V2977D-I2017C 0.233178706381514 D1256B-Y1304B 0.232697280121489 T1297B-L550A 0.232592836457788 S2764D-A2786D 0.232581648439674 F1254B-G1469B 0.231365208202547 G1996C-L2211C 0.231016170989763 A2215C-F1296B 0.229723736613862 P2003C-T2052C 0.229005295529554 I551A-Y556A 0.228983375263136 V2986D-V2026C 0.228083486128563 L2055C-A1306B 0.227654944267698 M1482B-I1487B 0.227594540830026 S2212C-S1302B 0.227120613849723 S2967D-Y2974D 0.226899249495073 P507A-Y556A 0.226817852263334 V1997C-I2220C 0.225403446397085 F2742D-N2968D 0.224918349066507 G500A-L719A 0.224900659454794 S2049C-S555A 0.224780727406019 Y2051C-F1295B 0.224451585489222 V2766D-M737A 0.223900093414798 I2756D-V725A 0.223784006203633 F2792D-Y556A 0.22361556339164 I553A-A558A 0.223270867743689 G1469B-F547A 0.22219417135212 Y2750D-V722A 0.221518135342247 I1301B-A1306B 0.220853482769749 I2797D-A2802D 0.220797892479725 A2011C-F2043C 0.220338686396168 G1469B-G1477B 0.220188648202826 S2050C-S554A 0.219750231563664 Y2974D-A2011C 0.219403673465567 M1482B-R539A 0.219155709349976 G1250B-A1468B 0.218648490476612 F2792D-A723A 0.218515478919822 G500A-D506A 0.218332274251284 Y520A-V525A 0.217766973311662 V2977D-G2038C 0.217699940324271 S2743D-S2049C 0.217241313439169 L2745D-N2968D 0.217037476430892 Y2800D-F2042C 0.216713165405691 A1485B-F528A 0.216502440160873 W2790D-T549A 0.215973299351488 F2000C-Y1304B 0.215912542654379 S2799D-S1302B 0.215666222496219 A1306B-A558A 0.215376960412264 S1465B-A515A 0.21502260458203 S2798D-S1303B 0.214495547681538 G2740D-D2746D 0.213091986323019 G2216C-I1300B 0.212581212934018 L550A-S555A 0.212404637822484 A2053C-S554A 0.21224075171139 V2217C-M1264B 0.210887853340359 S1253B-A1259B 0.209837375257354 L2980D-V2986D 0.209835203612282 V2217C-V1292B 0.20968677992402 I2752D-S718A 0.209605214703595 V2222C-F1296B 0.209370095607199 F2742D-V2972D 0.208713485518034 V2763D-S2781D 0.208552216376891 V2026C-S2032C 0.208334580112378 L2221C-T1297B 0.208109361706377 V2788D-G734A 0.207624964673292 L2227C-V1289B 0.207556536855443 V2741D-I2975D 0.206154733108173 F2973D-V2013C 0.204869011187218 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