[Wed Aug 24 19:06:57 2016] >Input file: TM4WD8ATD.pdb >TM segments: A25-42_A48-64_C25-42_C48-64_B25-42_B48-64_E25-42_E48-64_D25-42_D48-64 MAIDEN score = -73.9933 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- L59D-I62C 0.887735916771714 I62B-L59A 0.850443818517281 L59B-I62D 0.841241019429682 I62E-L59C 0.812919316787835 L59E-I62A 0.6025993000798 I62D-I62C 0.529776140473663 A55B-V54D 0.516319165667135 V54B-A55A 0.514701350499022 I62B-I62D 0.51208857981354 A55D-V54C 0.508198031958486 I62E-I62C 0.476869015824382 I62E-I62A 0.466350061394761 V54E-A55C 0.460892869148712 I62B-I62A 0.446179148473088 R27C-V32C 0.420504902039393 R27E-V32E 0.418625858800584 I62E-A63C 0.394489591541622 R27B-V32B 0.385780861745171 A63E-I62A 0.371486177931398 L59E-G61A 0.370410750228253 P56E-V54A 0.350391786291788 A55D-S58C 0.345748634800459 S58B-A55A 0.344615378315722 S58E-A55C 0.34363325188856 A55B-S58D 0.334661640388776 R27A-V32A 0.33205262937238 A55B-G61D 0.330501295513227 R27D-V32D 0.320266761555704 A63B-I62D 0.315468692120018 S41B-H51B 0.311183072735548 I37A-Y42A 0.305342596613615 A55D-G61C 0.305259267276339 S41A-H51A 0.296714220867242 A55E-A55C 0.279002597311865 A55D-A55C 0.272996459355737 S58E-P56C 0.272510626285891 G61B-L59A 0.267552883967063 G61E-L59C 0.265548537929889 A55B-A55A 0.264972668575008 A63D-I62C 0.261085713564919 I37E-Y42E 0.257688609508593 S58B-P56A 0.257185476219674 P56B-S58D 0.256965967085987 S41C-H51C 0.251319093087355 G61B-S58D 0.250354838966111 L59B-P56D 0.247701683305578 P56D-S58C 0.247028313581729 L59E-S58A 0.246641724494751 A55B-A55D 0.245976307302415 V54E-P56C 0.243742663126624 L59D-P56C 0.24353660348486 P56B-L59A 0.241486458354391 H51E-P56E 0.241236683688279 T53C-L59C 0.235676479133832 L59D-G61C 0.234244081976703 T53A-L59A 0.233844280260471 T53D-L59D 0.233563527099833 T53B-L59B 0.22697994372663 G61E-A55C 0.22640566366565 L59E-P56C 0.224750448206939 P56E-L52A 0.222888755670907 R27E-L33E 0.221194157291551 L59B-G61D 0.220668828140698 F57B-I62B 0.219661695623634 F57A-I62A 0.218663925074292 S58E-G61C 0.216976849957423 G61D-S58C 0.216524460431717 P56B-S35D 0.215688506310115 P56E-S35A 0.21480754037668 S35E-P56C 0.214687628675928 L52E-P56C 0.214367694170877 H51D-P56D 0.213727849545079 H51A-P56A 0.213004905793233 S58B-G61A 0.212793734412075 G61B-A55A 0.211420168699236 S58E-V54C 0.210100954353805 S58B-V54A 0.209328862630091 P56E-S58B 0.209157032506424 S58E-P56D 0.208947051605754 V54D-S58C 0.208026324002307 A55E-V54A 0.204208750114818 I64E-G61A 0.203987591625461 H51B-P56B 0.20224977258632 H51C-P56C 0.201105252646651 A55E-S58A 0.201000034916452 V54B-S58D 0.200231334887855 L59B-P56A 0.196127359012784 V54E-L59C 0.194135707880922 P56B-L59D 0.193962172637614 P56D-S35C 0.191418231982375 S41E-H51E 0.190851074447192 A38B-T53B 0.190744395108904 A55B-S58C 0.189142858836886 I37D-Y42D 0.188239413151582 L59D-V54C 0.183431990250872 S58A-P56C 0.177253884011075 P56D-V54C 0.176191790294494 P56B-V54D 0.176026631106638 P56E-L59C 0.175994272790993 P56A-S58D 0.175554881473249 P56D-L59C 0.172318987135554 Y42B-G49A 0.172303091326827 L60B-S58A 0.171596223274973 S41A-L52A 0.171290080210345 S58B-L60D 0.170574824488996 V54B-P56A 0.169606813106635 V54E-S58A 0.169593648434924 P56E-P56C 0.169257715119311 S41D-H51D 0.168542036109691 A38A-T53A 0.168061574306094 A38D-G49D 0.167331173063053 A38C-G49C 0.165083750073531 I50B-I39D 0.164832691342753 G49E-A38A 0.164230331741959 S58B-S58A 0.16415032035397 A38A-G49A 0.163346679840823 G61B-G61A 0.162613163236874 T53A-G61A 0.162414597458193 G61D-G61C 0.162314904066106 P56E-P56A 0.161434115031295 S41C-L52C 0.16118653227127 R27C-S35C 0.161099729288243 P56E-S58A 0.161022390245326 P56B-S58C 0.15903576882154 A55E-A55A 0.15876564384689 S41E-L52E 0.158715832324567 I62B-A63A 0.15846645733218 S58D-L60C 0.158215979731393 G61B-G61D 0.157810108909481 T53E-G61E 0.157723159630505 I62E-G61A 0.15715309309312 G61E-G61C 0.156265442031002 G61B-A55D 0.154237920015243 S58E-P56A 0.153969635366769 P56E-T53A 0.151306930072338 L60E-S58C 0.151042361273548 A63B-I64A 0.149799309051966 I37C-Y42C 0.148967351221969 R27E-S35E 0.14872057583909 G61E-G61A 0.148560959752834 F57E-A55A 0.140263499046293 F57B-G61D 0.139858216018682 I64D-A63C 0.138919897709086 I62E-L59B 0.138635007075027 A38B-H51A 0.13531365831282 I39E-I50C 0.133983907343589 I40B-L48B 0.131678384035889 L59E-L59B 0.131436695118679 V54B-L59A 0.131113271468548 A55B-G61A 0.13108987009238 L59B-V54D 0.130924624034764 L59A-L59D 0.13088326481251 S41D-L52D 0.130574852601026 L59E-L59D 0.130405982999785 F57D-G61C 0.129959863982712 S41E-I50E 0.126213128061508 A38E-T53E 0.126088529259621 F57B-V54D 0.125524036696907 G61E-F57C 0.125257806178066 A55A-S58D 0.124320891926869 I64B-A63D 0.122131564156843 L59B-L59C 0.121812425544048 G61B-F57A 0.121456311981591 I40C-L48C 0.12119669082705 A63B-L60D 0.119910400670586 I62E-A55A 0.119715736122463 L59A-L59C 0.118159951804184 A63B-L59D 0.117915325671983 G61D-A55C 0.11741984512182 T53E-L59E 0.116436672670325 F57E-G61A 0.116005455991677 T53B-S58D 0.115967044519571 L52E-S58E 0.115865361480028 L59E-A55D 0.115060583618771 A63D-L59C 0.115016704200388 L59E-L60C 0.114723950265351 L52D-L60D 0.114237045582741 A55E-A63E 0.11356378397618 L52A-L60A 0.113475122858274 A55E-A55D 0.11302095334228 L59A-I62C 0.11270806057012 L60E-A63C 0.112373359670694 L60E-L59A 0.11146198108847 A55E-L59B 0.110842492081317 I62B-I64D 0.110139610507447 I64B-I62A 0.110138631643484 S58E-T53C 0.109074119132284 A55A-A55D 0.108539239442232 A38D-L52D 0.108422769780752 A38C-T53C 0.107417902973462 A63E-I64C 0.107373236294625 A38E-L48E 0.107364784931482 R27A-S35A 0.107337295125651 T53D-S58C 0.10674124138721 L60B-L59D 0.105964516249002 I39B-L48A 0.105925586058679 P56D-A63C 0.105342122921992 S58B-T53A 0.104886093627841 G61E-A63C 0.104087432659739 A63E-G61A 0.103774000083511 P56B-A63D 0.103617122740535 A55C-A63C 0.10354356315982 L52A-L59A 0.10255958985638 S41E-L48E 0.102492457566865 A38B-G49B 0.102274286225248 A63E-S58B 0.101052887828747 A55E-F57C 0.100504432596286 A55E-L59A 0.100439961778163 S58D-S58C 0.100217693357214 A63E-V54A 0.100098137180784 L28E-A38E 0.100035358524946 L59B-A63A 0.0996578973609044 P56B-P56A 0.0993482918741568 S58A-A63C 0.0991059522432625 L60D-L59C 0.098353198484632 A55E-L60C 0.0981662190062539 L30B-A38B 0.0979287840763203 L52C-L60C 0.0976134174204009 F57D-I62D 0.0976025017313926 A55E-G61A 0.0974583117578707 L59B-L60A 0.0973414112293833 A55D-A63D 0.0971813957364343 A63B-P56A 0.0964246549737289 P56E-L59A 0.0961338053819323 L52B-L60B 0.0956153398869983 V54B-F57A 0.0951804216286414 L52D-L59D 0.0946134348199117 F57C-I62C 0.0941124286552224 I40B-L52B 0.0938017842234385 I39C-L48C 0.0938017842234385 P56D-L52C 0.0936749686734881 I62E-L60D 0.0934825606170603 P56D-P56C 0.0934495242906559 L52D-I39C 0.0930341990201453 L60A-I62D 0.0930073373501732 A55E-V54C 0.0926951586009831 L52B-L59B 0.0926716004086727 L59E-I64C 0.0926167446655387 I62D-I64C 0.0921998492153557 A63E-P56C 0.092103055162434 A55B-A63B 0.0918862306291435 V54D-A55C 0.0914415274211954 A55E-A55B 0.0905595248091342 V32E-I40E 0.0901268335883886 V32D-I40D 0.0900120859019909 V32A-I40A 0.0899875380183099 A63D-L60C 0.0893553697264475 L60B-I62C 0.0890555647361224 A55E-S58B 0.0888245873987472 S58D-A63C 0.0886750888101133 I37D-L52D 0.0884130183222172 L60B-A55D 0.0882629586488481 L60B-A63A 0.0881111869210573 L52C-L59C 0.0880686581214287 P56B-P56D 0.0876407715838683 L59E-A63C 0.086510726992751 A55A-A63A 0.0853983482919407 H51E-A38A 0.0853163137242703 L52E-L59E 0.0852483265825916 P56B-S58A 0.084531883819051 T53E-S58A 0.0842887154246643 L60D-A55C 0.084069243861316 G49E-I39A 0.0840093000366071 S58E-T53A 0.0839928756625337 A63E-P56A 0.0824261597715061 L59A-A55C 0.0823030002261218 S58E-A55D 0.0820112609971675 L52A-S58A 0.0819464897940869 I36E-I50C 0.080860077387759 V32B-I40B 0.0788563110261347 N31D-I39D 0.0785587798224057 A38D-T53D 0.0782764082745575 I37B-L48B 0.0782681832734657 I64E-L60A 0.0781072624912902 I40E-I50E 0.0771993369638193 V32C-I40C 0.0770380693179975 S58D-P56C 0.0769103506562514 P56E-S58C 0.0768153574475017 A55E-G61C 0.0763366595810804 I62B-V54D 0.0762836952375523 F57D-A55C 0.0757454759487679 I50E-A38A 0.0753417958339281 A55B-V54A 0.0751692951914609 A55B-L60A 0.0748025856273076 I50B-I36D 0.0745512349432729 S58A-A55C 0.0743966778738003 N31C-I39C 0.074208022025106 S35B-P56A 0.0739706496384702 N31A-I39A 0.073569180431728 V54B-A55D 0.0734613666656951 S58B-A63D 0.0728846423986216 S58E-S58A 0.0728034473954899 A55A-P56C 0.0712668069212681 P56E-I62A 0.0711843525295119 R27B-S35B 0.0707276601605757 L30B-I39B 0.0704215320983903 L59E-T53A 0.0695743124404624 S35D-S41D 0.0689447437988067 A63B-S58A 0.0683170184924133 S35A-S41A 0.0681908617514275 S58E-L59A 0.0680860690998051 S35C-S41C 0.0673182508718422 S35E-S41E 0.0672544155005685 H51D-A38C 0.0672301149790799 G49E-I40A 0.067192377863055 F57B-A55D 0.0669682354473959 G61E-I64C 0.0668932270621863 L52B-A55D 0.0667863449764381 F57E-L59A 0.0664661494769563 A55B-L52D 0.0664050107377744 S35B-S41B 0.0662528779576466 A55A-L59C 0.0661444660651811 S41A-I50A 0.0660301557556782 N31E-I39E 0.0659284987129416 A63A-A63C 0.0650664176807454 A55B-A63A 0.0650664176807454 A63B-A63C 0.0650664176807454 I50E-I40A 0.0650210139443894 I62B-L59C 0.0649328511539604 L52D-S58D 0.0645147070404846 N31A-I36A 0.0643538909805477 I62E-S58A 0.0641310652175138 F57B-T53D 0.0639632386470745 I62B-G61D 0.0636914980536538 I64E-A63A 0.0623145582530391 A63B-A55D 0.0618991648333598 T53D-G61D 0.0618281960117528 M34D-L52D 0.0614726376995252 A55D-L52C 0.0611983515945638 T53B-F57A 0.0609921260108957 N31C-I36C 0.0606341524818887 L52E-A55C 0.0600004581431579 I62E-P56C 0.0597313957068768 A38D-L48D 0.0594612047011367 A55B-A55C 0.0594577764122999 A63D-A55C 0.0593126990429669 L52C-S58C 0.0582906994669987 P56B-I62A 0.0577238544756349 I62D-P56C 0.0577238544756349 V54B-A63B 0.0570784876504646 N31B-I39B 0.0567308521261358 A38C-L48C 0.0558476046892098 I39D-L52D 0.0556795380793877 S41D-G49D 0.0554290576197056 N31B-I36B 0.0548059848590299 F57B-I62A 0.0544043449393465 A55B-L60D 0.0537517386460538 L52B-S58B 0.0527615985943928 I39C-L52C 0.0525454238083456 A38C-L52C 0.0525084649458227 I39B-T53B 0.0525060803422158 S58B-P56D 0.0524402892610052 I64E-I62C 0.052308321086584 R27D-S35D 0.0521352635861696 I40A-L48A 0.0521311695436566 V54D-G61C 0.0516795889094411 I37C-L52C 0.0515655401285615 S58A-L59C 0.051322307790008 S41D-I50D 0.0511392619359221 A38D-H51D 0.0510682309761177 S41B-L52B 0.0502193673103848 I25A-N31A 0.0496917495200551 A55A-A55C 0.0496383028377823 H51B-I39D 0.0495196049436976 L52B-A55A 0.0495086014697486 T53C-G61C 0.0485335864486931 L52E-A55A 0.0482196009017493 L33B-S41B 0.0482027231794715 V54B-G61D 0.0475738638146126 I40B-G49A 0.0473012553123371 V54B-I62A 0.0472887318132527 F57E-I62C 0.0472723003131108 I62D-V54C 0.0466140693059245 I40A-H51A 0.0465893992207859 I39E-H51C 0.0465742878620108 F57E-T53C 0.0462689323891272 V54E-A55A 0.0459969264715268 S58E-L60A 0.0459601920856514 A38B-G49A 0.0457547972636085 A63E-S58C 0.0454267290460457 G49D-I39C 0.0452863240400316 L52E-L60E 0.0443073561957998 V54A-A63A 0.0439509296449057 S41A-L48A 0.0436200454610267 S58B-S58D 0.0436042021678382 A38C-H51C 0.0431982564788048 L60E-A63A 0.0429517448369587 A55E-L60A 0.0427628635269934 A38B-L48B 0.0426699542471665 A38E-H51E 0.0426366963938921 A38B-H51B 0.0425191794687006 S41E-G49E 0.0422164642236668 P56E-G61A 0.0420925206242715 I62B-P56A 0.041998568792263 F57E-L60A 0.0419178398088693 T53E-F57A 0.0412768685788176 I37A-H51A 0.0409476584896519 F57E-I62E 0.0407936563632703 A55B-F57A 0.0399458065885847 I37D-I50D 0.0397317651956905 I39A-T53A 0.0395793756691116 F57B-T53A 0.0393025409069372 S58E-G61A 0.0384345861442075 H51B-F57B 0.0378904001390996 S41B-G49B 0.0378803148405207 L59E-S58B 0.0370488755433487 S41A-G49A 0.0364602938708468 P56E-I62C 0.0358641609051098 L60E-V54A 0.0355839135573124 T53E-P56C 0.0349114658786309 M34B-L52B 0.0346440667222701 L59B-S58C 0.0340747906177825 I62B-F57D 0.0338265065461751 L33A-S41A 0.0335795459548898 A55E-L52A 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