[Wed Aug 24 19:10:31 2016] >Input file: TM4WMZCGD.pdb >TM segments: A11-70_A81-129_A144-175_A191-256_A299-317 MAIDEN score = 12.4952 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- F87A-M94A 10 F85A-V96A 10 P51A-S86A 1.53203816179405 I84A-V96A 1.40255177771095 W59A-L90A 1.19151034511666 D83A-L98A 1.18182929872828 F33A-W56A 1.14108847813979 H103A-N108A 1.11413819918211 I37A-W56A 1.07633902348768 V60A-L89A 1.05695568680478 F85A-Y97A 1.01353208045077 P126A-R248A 0.992488110075181 K214A-A220A 0.985948655908828 K228A-H244A 0.943330035669644 L52A-S86A 0.935143331696261 L222A-K247A 0.913360755438386 I199A-Q310A 0.906326851400626 W59A-V88A 0.893639771424425 L222A-D227A 0.888678203620971 V60A-R92A 0.880803276476804 L226A-D249A 0.863123983099465 V127A-D249A 0.850092632764845 V88A-V93A 0.816991389134749 V60A-L90A 0.812496674960617 V60A-V88A 0.81177062011643 H122A-P238A 0.793276921811745 L98A-H103A 0.79154282478432 W59A-L89A 0.748278271973507 L226A-Q252A 0.734418996226491 V88A-M94A 0.728884222804027 W59A-G91A 0.710830743975118 S62A-V88A 0.701759057276606 V64A-K69A 0.685889773145535 F87A-V93A 0.683710425886093 V127A-L226A 0.679943148996291 I84A-L98A 0.678657214041835 D225A-H244A 0.678123830661693 F128A-I303A 0.666526200914614 L222A-Q252A 0.661888401174789 F85A-M94A 0.652488383426001 P51A-F85A 0.650734473959722 P232A-P238A 0.650034882734685 V53A-F87A 0.631734652531019 Q40A-L52A 0.625913352499886 F128A-A299A 0.609772833399443 V161A-E166A 0.592350450649261 L240A-R246A 0.588203283285519 V127A-R248A 0.586532417820379 L123A-P232A 0.580543603729267 L163A-S205A 0.574058306878071 Y223A-A251A 0.562190474869817 L152A-F157A 0.554311420044479 A202A-L207A 0.545132397717586 S26A-I31A 0.539489234623399 I37A-Y55A 0.536553110269718 I31A-N36A 0.534298274108684 P50A-F85A 0.528431602630068 P241A-R246A 0.526485215407921 P126A-Q252A 0.524778411329189 D225A-H250A 0.519220603944676 I84A-Y97A 0.517800295639682 H122A-H244A 0.516091203872639 A63A-V88A 0.515181732435656 L89A-M94A 0.513335658716608 V168A-R173A 0.510707070155399 L123A-F230A 0.506780916604209 N36A-I57A 0.504375474623354 F128A-D249A 0.503637735700631 E167A-F200A 0.503532007826614 S44A-R49A 0.503469204353843 F200A-R212A 0.502071129322348 E167A-P195A 0.495087847846019 E155A-P162A 0.49336472067719 L226A-K247A 0.493237060435542 N239A-Y245A 0.486794497757429 L207A-R212A 0.485367392112601 E104A-A109A 0.484725705761922 K149A-K154A 0.484514438644429 P126A-D249A 0.482729790348999 D225A-F230A 0.477400706740998 S203A-M211A 0.472444104572996 L240A-Y245A 0.464343689348299 Y97A-F105A 0.461780077700256 I34A-W39A 0.460658019987264 Y43A-L52A 0.460495850122164 H122A-F237A 0.459502779222706 P238A-E243A 0.459000452121237 L17A-R24A 0.45747455837078 F87A-T95A 0.455024415644082 K228A-Q252A 0.453050491477817 P241A-K247A 0.444221914755171 S44A-L52A 0.44336626780855 S86A-V93A 0.442395312332781 S62A-F87A 0.439314105396734 I11A-F16A 0.436849749970753 N36A-W56A 0.433551968584537 L52A-F85A 0.430141749120672 Q194A-A314A 0.429459205023982 L222A-R248A 0.423953498317093 L52A-F87A 0.423331185898134 E210A-T217A 0.418879242166815 Y160A-A165A 0.417030368983321 L123A-P238A 0.416352503585566 F128A-L302A 0.415258806530241 P126A-K253A 0.414650113223005 D249A-A254A 0.410490606704952 F128A-L301A 0.409889225045315 I164A-M197A 0.407867819101074 V127A-D227A 0.405813688947578 A202A-L306A 0.404032900350844 L163A-T201A 0.401469540584783 L123A-F235A 0.400398321260464 V305A-Q310A 0.398577846038085 L41A-R46A 0.397002389948662 W56A-G61A 0.396837468655878 I164A-F200A 0.396716126439353 F54A-G61A 0.395349520631656 Y55A-S62A 0.386845580743842 Y43A-R49A 0.384408437943878 S86A-M94A 0.383964045375686 F33A-P58A 0.383140787846826 P232A-F237A 0.382572246003414 Q194A-H311A 0.380420381683243 T198A-S313A 0.379988953272386 A202A-A314A 0.379174376146328 R246A-A251A 0.379060007018885 Y169A-Q194A 0.375338904030649 D83A-H103A 0.370751748189267 D227A-H311A 0.369528215685427 G61A-V88A 0.366213514224134 Y223A-K228A 0.364416412540158 Y43A-D48A 0.362537091399683 G61A-L90A 0.360389039887597 H244A-D249A 0.358358361944742 P162A-S203A 0.356702094992089 Y55A-V88A 0.355050485943256 A63A-L90A 0.354837192025176 V236A-Y245A 0.350304276092331 Y120A-G304A 0.347463570702733 Y35A-Q40A 0.347408814068571 P126A-P241A 0.345177511540422 Q40A-P50A 0.344458374621182 Y169A-D174A 0.344206029657692 E104A-K110A 0.343639243911516 A63A-L89A 0.343451893877332 Y55A-V93A 0.342472279821636 L302A-M307A 0.341450182028542 L226A-R248A 0.340252762454316 K228A-P238A 0.339919614571172 P126A-K228A 0.339514674981212 I30A-Y35A 0.338982403961523 N36A-P58A 0.338164352276281 S224A-K253A 0.333616166610339 K214A-S256A 0.33350986883304 E117A-F235A 0.332809387554769 P126A-K247A 0.332561698812988 K228A-R246A 0.330932458925649 K47A-S86A 0.330917504454916 K154A-S205A 0.3297354795788 K228A-H311A 0.328922122114455 Y66A-V96A 0.327322436403172 I164A-R204A 0.323883379709802 I164A-S205A 0.322866532529089 A151A-L206A 0.322402488988406 E167A-Q194A 0.321918345959271 V168A-M197A 0.320988660484077 N36A-Y55A 0.314810024021307 F33A-I57A 0.314369306611507 P195A-S203A 0.313688279693224 R24A-I30A 0.313002863951588 Y221A-R246A 0.309874683918217 D227A-H250A 0.309145881712535 F237A-E243A 0.304377105986893 Y223A-V305A 0.304057989058987 P51A-I84A 0.301453716401875 V127A-N300A 0.301138698049613 P126A-G229A 0.300351336982734 N234A-R248A 0.299591948115327 L13A-A18A 0.298221986519491 P195A-Y221A 0.296533154503547 Y66A-R92A 0.295630563260061 T125A-P232A 0.293337765996895 S86A-V96A 0.289916260691444 I84A-T95A 0.289532686297366 K149A-S205A 0.28921363250447 S62A-L89A 0.285840788320124 T198A-A314A 0.285351572301338 L222A-H250A 0.284418674289087 Y120A-G229A 0.284249134725103 P126A-Y223A 0.284096203984387 F128A-G304A 0.282955280004566 L222A-A254A 0.281608291223277 P232A-Y245A 0.281322096791039 V53A-D83A 0.281183721469619 E167A-T193A 0.281134842225919 V127A-I303A 0.281114781383709 E166A-R204A 0.280348815670433 K228A-Y245A 0.280106545306812 E166A-T198A 0.279066809588305 R173A-M197A 0.278980084571689 S203A-G309A 0.278518273290572 L123A-G304A 0.276212324283199 Y66A-V93A 0.274838373224014 K228A-E243A 0.269461827935096 T201A-E210A 0.26682161217957 A165A-A202A 0.265558830002094 H122A-Y245A 0.265079807801403 F87A-R92A 0.265079668041188 D227A-G308A 0.264813971565885 G229A-H311A 0.262936613703435 L52A-G61A 0.262671866755471 L226A-A251A 0.261995561856493 R212A-Y223A 0.260005637934931 V127A-A251A 0.259880724847793 G229A-L240A 0.25980861816694 Y55A-G91A 0.258509821940279 Y223A-G308A 0.257778583176409 L27A-P32A 0.257236521523646 E167A-F172A 0.257114460814609 T193A-T312A 0.256478862706465 I233A-P238A 0.256446785615178 T201A-L206A 0.25630494801513 Y223A-H311A 0.255577645548381 P50A-I84A 0.254086421251748 M197A-R212A 0.248957600493736 R49A-M94A 0.248674658019066 G150A-F157A 0.248270806215459 D48A-D83A 0.248261602472252 D218A-D227A 0.246791003337369 S224A-Q310A 0.245968404706249 T198A-Q310A 0.245933656235152 Y221A-H244A 0.245772495733399 R173A-Q194A 0.244502136586818 Y97A-G102A 0.242323930805101 L123A-N234A 0.242319796495963 T201A-L215A 0.241772399044662 G229A-G308A 0.240475308235656 R46A-P51A 0.239884725986789 A220A-K247A 0.239791828778212 F85A-G102A 0.238317647255235 T231A-P238A 0.237859593267249 L163A-S203A 0.236140916128271 Y43A-F85A 0.235974826209159 L222A-H244A 0.23518599983275 E167A-M197A 0.235081363297407 T198A-L206A 0.234185577985563 K170A-R212A 0.233845334276038 Y43A-S86A 0.233275803196712 G61A-L89A 0.232789598709974 I57A-S62A 0.232091646251483 Q310A-A315A 0.231508191362636 S175A-Q194A 0.230018290898108 P51A-L98A 0.229730865270994 P126A-H244A 0.22954882707577 K170A-D216A 0.22837327086203 F105A-K110A 0.227945714618953 K158A-R204A 0.227414072175832 D225A-Y245A 0.227123541543269 T125A-L226A 0.226703079140905 L13A-P20A 0.22662168956748 P50A-T95A 0.226471831519239 R246A-K253A 0.225958598204543 L242A-K247A 0.225806958951074 A165A-S205A 0.225061719447226 K228A-N234A 0.224973988721726 M191A-A314A 0.224524485788149 G102A-F107A 0.223709951303935 E155A-Y160A 0.222513737113696 G82A-G102A 0.221377601112663 M197A-A202A 0.220104515338013 R92A-A119A 0.220051184251524 P70A-L98A 0.219584302098776 S203A-A220A 0.218016156022426 F16A-A22A 0.217622811090335 Q194A-S317A 0.217616634672351 I255A-A299A 0.217411579221642 T231A-H311A 0.217327097793738 S224A-S256A 0.217084446319504 E196A-T312A 0.214736207300875 A220A-K228A 0.214615385302041 G129A-I255A 0.214400112662711 A202A-K214A 0.213541606311484 Q252A-M307A 0.212151186135086 E166A-Y171A 0.20898266956129 Y120A-T125A 0.208806272545464 L123A-G229A 0.208537660021607 H122A-F235A 0.208224912668681 L123A-T231A 0.206390427564098 T198A-G208A 0.205712157314112 S62A-P232A 0.20542090886847 L123A-V236A 0.205103977721431 I199A-V305A 0.204743263597008 V53A-V88A 0.20419578054177 T198A-I303A 0.203736182942161 D225A-A254A 0.203449699380224 I29A-W56A 0.203165743689593 D48A-M94A 0.202992296630339 I199A-G309A 0.202855189045588 P58A-S86A 0.201777009694173 M197A-L206A 0.200276811020116 T125A-N239A 0.198696104070804 S203A-S256A 0.198401983524586 T231A-N239A 0.19690305776228 A116A-H122A 0.195199752089609 P32A-G91A 0.194710314324396 T95A-H103A 0.193472557566101 T193A-H311A 0.193114800216535 P70A-T95A 0.191717479492031 K247A-Q252A 0.191652298395114 N108A-D113A 0.190930844845728 L21A-S26A 0.190793823248431 D218A-A254A 0.189944520964632 Y221A-G229A 0.189619997094382 K214A-S224A 0.189162856782203 P126A-L302A 0.18902629247872 I303A-G308A 0.18823057718037 Q144A-K149A 0.188129422423893 F172A-Q194A 0.187474714406727 T217A-A254A 0.18737802725226 V65A-F87A 0.187207468248777 Q194A-M307A 0.18667002024284 A119A-T231A 0.186467772396041 D218A-Y223A 0.186215581393752 G61A-F87A 0.185940489106567 M211A-Y221A 0.185478448375648 A156A-R204A 0.185475453742174 L226A-R246A 0.185035359923635 F230A-N239A 0.185017225355239 Q23A-P32A 0.184921630218402 E196A-K214A 0.184678422253203 T95A-A119A 0.184426367661228 T124A-D249A 0.184128578760459 N300A-G308A 0.183688052051236 E196A-Y221A 0.182621499313769 K253A-L302A 0.182561966007662 H103A-K110A 0.182428538667344 L41A-K47A 0.1818496859766 A121A-F237A 0.181116188248764 F147A-T153A 0.181048553207736 Y81A-F87A 0.181027509728935 H311A-T316A 0.180954583046923 Y223A-G309A 0.180696573836266 Y81A-G99A 0.180010460111883 A202A-I303A 0.179831279613158 Y55A-M94A 0.179307424263572 L207A-G304A 0.17929447495665 V38A-Y43A 0.179015231974484 D218A-R248A 0.179001305200541 L98A-E104A 0.178580638470986 F147A-N300A 0.178557880407262 G61A-M68A 0.178343235763301 L41A-P51A 0.178200689955908 P70A-Y97A 0.177993135504692 G61A-S86A 0.177763166903357 R173A-D216A 0.176633511683874 I164A-Y169A 0.176332148097226 D174A-R212A 0.17599203221259 K228A-L240A 0.175833299063724 T125A-H250A 0.175782140391972 F128A-K228A 0.175755005593285 E166A-K209A 0.175681524638984 A220A-D227A 0.175588259223479 L215A-A220A 0.17531326255807 N36A-G91A 0.17526277610208 Q40A-Y55A 0.174770036760366 V53A-R92A 0.174705385032224 F128A-G308A 0.174478568407839 R246A-A254A 0.17419664239017 P32A-Q40A 0.17380167223136 F87A-V96A 0.173433056538804 M197A-S224A 0.172830417321486 P126A-N234A 0.172710657440414 W39A-P58A 0.172475075007442 Q194A-A315A 0.171701807823281 F235A-H244A 0.171535018726389 G229A-R248A 0.171345185632824 S203A-Y223A 0.170676904685906 T198A-A220A 0.170469166457459 D83A-G99A 0.169517013376987 F54A-V88A 0.169303639007047 S26A-P58A 0.169127824001305 N234A-E243A 0.168547226881023 V127A-S224A 0.168519244442354 V127A-F230A 0.167960049168677 Y55A-R92A 0.167555633594841 Y223A-R248A 0.167500625685963 T231A-K247A 0.167434466830062 R248A-S256A 0.16740125957116 L163A-M211A 0.167238677959741 E167A-R173A 0.167109186544638 D225A-T231A 0.167071712913258 Q23A-I28A 0.166725113607836 S44A-Y81A 0.166527599363724 A202A-G304A 0.166388189479406 T125A-K247A 0.16613941161414 G309A-A314A 0.166124141677831 D113A-A118A 0.165944705686122 G129A-A251A 0.165884640282094 Y221A-K253A 0.165570013936176 P195A-H311A 0.165535920737631 S159A-K209A 0.16531120257249 D174A-T193A 0.165167031157936 Y245A-K253A 0.164717132173648 A118A-G129A 0.164078033385937 G304A-A314A 0.163929723325236 L222A-A251A 0.163517019867645 G82A-L98A 0.162859107292045 G229A-A254A 0.162798819005669 P241A-R248A 0.162690310090421 L206A-G309A 0.162336038381003 G229A-P241A 0.162083933417294 P126A-S256A 0.161979731207427 K158A-T201A 0.161872658410434 A121A-G308A 0.161636831452177 W39A-R46A 0.1610316000537 Y97A-N108A 0.16040973869273 R49A-V96A 0.159775671914692 G99A-N108A 0.159284768554489 T201A-L207A 0.158771680019657 E167A-A213A 0.158648777311384 F200A-A314A 0.158631081356709 T125A-D227A 0.158559388161472 S203A-N300A 0.15821552642413 E196A-G309A 0.158102147365659 S203A-R212A 0.157859369167873 A116A-T125A 0.157733863206369 K170A-R204A 0.157414678884982 F128A-S224A 0.157319366656237 N36A-L41A 0.157078639631099 A220A-K253A 0.157064665649681 F219A-K247A 0.157007175527525 N234A-N239A 0.156114959073635 K47A-D83A 0.155891297839022 D227A-K253A 0.155835019284851 K158A-L206A 0.155625543642775 A202A-Q310A 0.155501567390377 K149A-L301A 0.155405915232919 Y35A-F54A 0.15504586175094 V148A-A299A 0.154908338210932 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