[Wed Aug 24 17:20:01 2016] >Input file: TM4X5TATD.pdb >TM segments: A220-236_A258-276_A279-295_C220-236_C258-276_C279-295_B220-236_B258-276_B280-295_E220-236_E259-276_E279-295_D220-236_D259-277_D279-295 MAIDEN score = -92.8761 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- L224C-I229C 1.29283166901432 L224A-I229A 1.27308054072387 L224D-I229D 1.27059052109552 L224B-I229B 1.18660729186338 L224E-I229E 1.1718746875351 T262B-L261C 1.14956190485381 I234C-M263C 1.08668396002768 V280B-I285B 0.996916183988293 V280E-I285E 0.995057478413038 I234B-M263B 0.994722117842091 I234D-M263D 0.993735980855721 V280A-I285A 0.933897152625375 V280D-I285D 0.920047573489261 L274C-D284C 0.882157838989581 V277D-D284D 0.856235841369729 L274B-D284B 0.852960574997236 L261A-T262E 0.847423013181931 L274D-D284D 0.828353800995936 I225B-D284C 0.809925353429912 I225D-D284E 0.808867374962419 V280C-I285C 0.781937938143276 D284A-I225E 0.760656943585938 L291A-P230E 0.757739128700727 I234B-T262B 0.748398564949804 I234A-T262A 0.731074402571267 I234D-T262D 0.728333375370657 T262D-T264E 0.722167216442806 T262B-V260C 0.715668581975909 T265D-T265C 0.715029937745336 Q226A-D284B 0.690951518625787 I234E-T262E 0.685469481084038 L274A-D284A 0.678700351081529 P230D-Q266D 0.654976349032406 D284D-I225C 0.653452995184145 S267A-G269E 0.651106150183379 F295A-L233E 0.648963531088228 P230E-Q266E 0.640295571313293 I234E-M263E 0.636578686663615 V235D-T259D 0.634429006520675 L274E-D284E 0.632985073912625 D284D-Q226C 0.601203338106063 T265D-T265E 0.600187483333658 P230C-Q266C 0.598271434445237 P275E-D284E 0.593236429786237 P275A-D284A 0.589770685188326 R271A-G269E 0.582921513217161 G221D-Y279E 0.569642844798159 V235B-T259B 0.568967769798951 Q226B-D284C 0.565509450691547 K281C-W286C 0.560121516004864 Q226D-D284E 0.553413902840163 T265A-T265B 0.553315413904874 I234E-T259E 0.550957974981561 D284A-Q226E 0.542766205817381 I225A-D284B 0.542737429075531 K281B-W286B 0.541986554575424 P230A-Q266A 0.538857778982389 T264D-T262C 0.538760850883193 K281A-W286A 0.537658639010459 I234C-T262C 0.53228817031084 K281D-W286D 0.52889709508995 L233A-F295B 0.526042777069252 S268A-S270E 0.525741988631466 P275C-D284C 0.522749354894946 S268D-S268E 0.52093287015739 P275B-D284B 0.520191846070368 S270A-S268B 0.518293851279655 S231B-S267B 0.517461797013743 P230B-Q266B 0.511758989551078 S268D-S268C 0.503608288407688 L233B-F295C 0.500578973626695 S270D-S268E 0.494672050487702 G269D-S267E 0.490588992730083 G221B-Y279C 0.490369905743925 T264A-T262E 0.487677510151096 P275D-D284D 0.4859573368386 S270B-S268C 0.484473338284005 K281E-W286E 0.483938754146899 I234B-T259B 0.478858952940012 G269D-R271E 0.470614553676735 Q226D-M287E 0.464429106144495 L224C-P230C 0.464117267565059 S231C-S267C 0.460964136710389 S267D-L274D 0.458922872767939 I225D-P230D 0.452620046463059 Y223E-Q266E 0.450431108323844 I225C-P230C 0.44874933427174 G269D-T265E 0.448019251151703 Y223B-Q266B 0.44753940982709 S267E-L274E 0.446230023191793 Y223C-Q266C 0.44241824377243 F295D-L233C 0.441654798702575 S267B-L274B 0.439574016120171 M227D-S270D 0.436233142512924 I225A-P230A 0.434175204961858 S268A-S268E 0.433611444638812 I225E-P230E 0.433542081887528 I234A-F295B 0.432942025824768 Y223D-Q266D 0.432543554446049 M227A-S270A 0.430802472990387 G269B-T265C 0.42895414760689 S267C-L274C 0.42863026714181 S267A-L274A 0.426595622189816 T262A-T264B 0.426526305089448 S231E-M263E 0.426521695047375 L224A-P230A 0.42249277558472 P230D-A288E 0.421640647997314 M227C-S270C 0.421315111196755 L233D-F295E 0.420689541623089 V235A-C290A 0.419409736750858 V235C-V260C 0.41738665883008 I225B-M287C 0.416457862355659 P230A-L291B 0.415471272436313 V260A-T262E 0.414298917493708 M287A-I225E 0.414184366578991 T265A-G269E 0.413460529567827 M227E-S270E 0.405988873103807 Y223A-Q266A 0.405964335766836 T259C-V294C 0.404778441685929 A288A-P230E 0.402059701531023 S267D-G269C 0.400586620821104 S268D-S270C 0.399571717451169 L292A-P230E 0.394885308877399 M263B-W286B 0.393982464044004 M263C-W286C 0.393879242345087 L291D-P230C 0.393039978373054 M263D-W286D 0.392907670204827 K276D-Y222C 0.392227223476641 T264B-G269B 0.38701430597594 V235D-F293D 0.386755810882487 A288A-I225E 0.385651340850435 I225B-I285C 0.38551522860654 I285A-I225E 0.384592708374672 R271D-G269C 0.384145330135064 I225B-A288C 0.382231114485316 A288D-I225C 0.381918509917352 T262D-T262C 0.381662106048238 K281B-C290B 0.38051760461789 S231D-S267D 0.379454355753719 Q266A-T264B 0.378925005810148 G269A-R271B 0.377869914880745 A272B-A272C 0.377680231260199 M263A-W286A 0.376657039109461 A272A-A272B 0.376596560895257 A272D-A272E 0.374099829482837 Y222D-D284E 0.372979062106622 Y222A-D284B 0.37289219697356 D284D-Y222C 0.372578385893699 K276D-Q226C 0.370221310150146 Y222B-D284C 0.369828880047258 Y222B-K276C 0.368306883901217 K281A-C290A 0.367760926195712 M263E-W286E 0.36773752965008 I285D-I225C 0.366976260042903 T265D-G269C 0.364042780954319 K276A-Y222E 0.362317959475678 S273A-P275B 0.362259605407368 T262A-T262B 0.361942687126837 S231D-M263D 0.358932349776734 T262D-T262E 0.358489901731016 Y222A-K276B 0.358252663431408 T262A-C290A 0.358017941307195 A272D-A272C 0.357418854149604 K281E-C290E 0.357147947776389 G269B-S267C 0.357073825890926 T259E-V294E 0.356717880620064 T262D-C290D 0.356572349310439 D284A-Y222E 0.356281953526933 M287D-Q226C 0.355915912385694 I234C-T259C 0.354855030585776 Y223C-I229C 0.353872707603462 T265A-L261B 0.353462463586885 V235A-M263A 0.35345669288824 L261D-T265C 0.35343184735606 V235E-M263E 0.353390241039477 Y279D-D284D 0.34915932834941 V235D-V294D 0.348710633495783 K281D-C290D 0.348673097839861 V235E-V294E 0.347819459238467 V235A-V294A 0.346338337131374 M227E-W286E 0.345457088618362 T262B-C290B 0.34525785201046 I225D-I285E 0.344998625503941 F295A-I234E 0.343082906157074 V235B-V294B 0.342764947756253 I234A-M263A 0.338676435808992 T262B-T265C 0.336939775835091 T262D-V260E 0.336858206673562 S267A-P230E 0.336851966956541 T265D-L261E 0.335714116765704 M263A-C290A 0.335655152563138 T258B-L261C 0.335053063353455 S270B-K276B 0.333600018906377 S268A-S268B 0.33295756728811 S273B-P275C 0.331908158541244 L261A-T265E 0.330196603820729 T262E-C290E 0.329525118367963 S231B-M263B 0.328811464730737 P230D-L291E 0.328501129989194 Y279E-D284E 0.327945561124529 S268A-P275A 0.327148880167388 P230B-L291C 0.326867086968486 L261B-L261C 0.326827965663618 K281C-C290C 0.325430503447575 T259B-V294B 0.325202343681761 S231E-S267E 0.324919006570763 Y279C-D284C 0.323367167024732 T262C-C290C 0.321933463908355 T264D-T258C 0.321043138709881 V235A-V260A 0.320503333709722 S270D-A272E 0.319547261013098 V235B-F293B 0.315461952622579 L291A-L233E 0.31521824461955 S270C-K276C 0.314469160517359 S273D-P275E 0.314369887664153 T264D-Q266C 0.312748789253516 S270D-K276D 0.311735696586652 Q226B-K276C 0.311100208270074 A288A-S231E 0.310914923277214 A272A-A272E 0.310598918639952 Y228E-R271E 0.310188745445095 Y228C-R271C 0.308472564196191 T264D-T262E 0.308087076118059 V235C-F293C 0.30616970151469 M263D-D284D 0.304788562609923 P275D-S273C 0.304734634697118 D284A-M220E 0.304701719018478 M263A-D284A 0.304680334773695 S231A-M263A 0.304292414348591 Y223D-P275E 0.303992663970588 Y223A-P275B 0.303815676609781 M263B-D284B 0.303587059954467 L233A-L291B 0.302978372652516 T258A-T262E 0.302568055784631 T265B-L261C 0.302380716530108 S270E-K276E 0.301716226768394 M263C-D284C 0.301489227144485 Y223A-R271A 0.301310972679223 L261A-L261E 0.301011528230988 M220B-D284C 0.300722617252609 L233B-L291C 0.300258731714297 P275A-S273E 0.299988361917659 Y223A-P275A 0.299852787920229 G269A-S270E 0.299810848780816 S231D-A288E 0.299442764758646 S270A-G269B 0.299082976982533 Y223A-I229A 0.29846652222283 S270B-A272C 0.297942799649287 P275A-Y223E 0.297080118631056 T262B-T264C 0.296293803871535 G269A-T265B 0.295613838539881 M220A-Q226A 0.295283432720342 I225B-P230B 0.294951993964625 M227B-S270B 0.294779661789657 T259A-V294A 0.294414804564691 T259D-V294D 0.293878612675884 G269B-S268D 0.293840869699568 S268B-G269C 0.29378885921578 S270B-G269C 0.293165402385277 M263B-C290B 0.293013476084409 S268A-G269D 0.292134401896045 P230C-T264C 0.291709145958911 M263D-C290D 0.291432116047897 M220C-Q226C 0.291200779614952 S270A-K276A 0.290761003678687 G269D-L274E 0.29044773574726 Y223B-P275C 0.289608266618739 Q266C-M287C 0.28943467484948 Y223C-R271C 0.288591631304852 G269A-S268C 0.287605367207565 Y228B-R271B 0.287525760957964 D284D-M220C 0.287497841465451 M220E-Q226E 0.286563960300626 T262A-V260B 0.286250783455004 Y223D-P275D 0.286066084875425 T265B-T264C 0.286063117868533 K276A-Q226E 0.285534689971183 M220D-D284E 0.285308561941647 L274D-G269C 0.285256131918194 M220A-D284B 0.283566810989643 M263C-C290C 0.283391042081942 M220D-Q226D 0.281927388130967 Y223E-R271E 0.281093135464972 P275D-Y223C 0.280858076486936 M263E-C290E 0.280836705039373 Y222D-K276E 0.278977415956458 M220B-Q226B 0.277911972769802 Y279A-D284A 0.27613086022095 M263E-D284E 0.27594160143614 V235B-M263B 0.275723138167477 T265A-T262E 0.275657194980939 S273A-K281A 0.275413303665083 T258A-T264B 0.275043311164371 S268C-P275C 0.274937102182061 L261B-G269B 0.271899060944025 T258B-T258C 0.27103321650587 Y223B-P275B 0.270241352538227 Y223D-I229D 0.267848790196783 T259A-T265E 0.267471706632416 V280D-I225C 0.267403920462194 S268A-T265E 0.267276784746358 G269B-R271C 0.267212529717781 Y223C-P275C 0.266809927082288 Y223D-R271D 0.266486374166743 L261D-L261E 0.265359285890953 S273E-K281E 0.264000395840336 S268C-L274C 0.262766866986849 S268E-L274E 0.262269291464793 Y228A-R271A 0.261914976669344 T259E-T264E 0.261615556338462 M227B-V260C 0.26011919243313 Y223E-P275E 0.259793495684832 I225D-A288E 0.258804770335018 P230C-T259C 0.258053427167753 L291A-S231E 0.256189765571698 Q266A-S268E 0.255585716847732 V260A-V294A 0.25457137706481 T262D-T264C 0.254143496035585 L274A-G269E 0.253967353736192 Q226A-S267A 0.253370199048119 Y228C-S267C 0.252375232348753 Y222D-S273D 0.250613328797348 C290E-F295E 0.250127310930976 M263D-R271D 0.249491466553076 Q266D-T262C 0.248773534962663 S268D-L274D 0.248771534474703 P275B-W286B 0.248741649631675 Y222E-S273E 0.248673624717376 L291D-L233C 0.248418563361832 Y222B-S273B 0.247575551709359 Q226D-K276E 0.247493557465766 S268E-P275E 0.246885037272701 T265A-T259E 0.246714614001228 T262B-T265D 0.246652793943797 C290A-F295A 0.246547578383799 Y223B-R271B 0.245989968878933 T259B-T265C 0.245952812485081 T258A-T265B 0.245906101850891 S267B-A288B 0.245818405484334 R271D-M287D 0.245698741152185 L224D-P230D 0.243901393921131 S270D-G269E 0.243473796246956 M263A-R271A 0.243414320284918 C290C-F295C 0.242714428150144 P230E-T264E 0.242171432034101 L232A-T262A 0.241947097964209 P275D-W286D 0.241439598428132 M263A-V260E 0.241371597743713 L261D-T265E 0.241104903029043 T265D-L261C 0.241038259693404 T262A-T265C 0.240985542024382 Q226B-M287C 0.240800362838655 S231C-M263C 0.240548650656642 T262A-Q266B 0.239739337673795 L232D-T262D 0.239658921885832 Y223D-A288E 0.238941238036522 R271C-M287C 0.238846839768527 M263B-R271B 0.238655104770628 A288D-Y223C 0.238229375760019 A288D-S231C 0.238165490755003 M263C-R271C 0.238027173245093 V260B-M263C 0.237578925785391 P230A-A288B 0.237523894723424 T265A-T262D 0.237486853847686 C290D-F295D 0.23737580503547 T265B-T264D 0.237000632407087 T258A-L261B 0.236414163029846 T262A-T265D 0.235602260565623 Q266A-S270E 0.235541882630962 A272A-S270E 0.235273062456756 R271B-M287B 0.235161315503458 Y228D-V289D 0.235017893312568 T262D-Q266E 0.234986476051406 T262A-T264E 0.234949069022129 T264B-T265E 0.234583978189899 Y223E-I229E 0.234286590904157 Y223B-S270B 0.234066126172881 R271A-M287A 0.233586489772957 V235E-V260E 0.233579223277431 Q266D-T264E 0.232216036818484 L261A-T262B 0.231095179390238 T265E-T262C 0.230691297088625 Y228E-S273E 0.229418499240321 S273B-K281B 0.229307444071688 S231D-S268E 0.228618797055838 Q266A-Q226E 0.22797477127804 S273B-S267C 0.227748596826189 L274B-I283B 0.227562958933927 S273D-S267E 0.227423674065468 M263E-R271E 0.227420658334583 Y228A-S267A 0.226951175607526 V235B-C290B 0.22592021118569 I236E-V260E 0.225433411847874 R271E-M287E 0.225202333060471 T264E-T265C 0.224260560079729 S231A-S268B 0.223884565032648 Y223B-I229B 0.22346643678414 I234B-V260B 0.223380641977136 V235E-C290E 0.223325875665095 I234E-V260E 0.223316659893855 L261D-L261C 0.222780091766945 T259A-T262B 0.222712910264899 V289D-I229C 0.221839529051635 G269B-A272C 0.221787533895283 Y228D-R271D 0.221213778551771 S231B-S268C 0.220815669242748 G269D-Q266C 0.220716554938801 S231A-S267B 0.219881732724627 Y228D-P275D 0.219434412484318 P275C-W286C 0.21927913927761 A272D-S270C 0.2191905255763 S267E-A288E 0.219114006290794 S268E-G269C 0.218497090643019 S268D-S231C 0.21849276535532 P230B-S267C 0.218151202056609 S267D-S273C 0.217960181267322 Q226D-C290E 0.217940501898806 T264A-T265D 0.217797826263125 I283D-A288D 0.217698179273094 T265B-T262E 0.217588785078121 S267C-A288C 0.216803768037378 I229D-C290E 0.216592439971266 Y223A-D284B 0.215911999634763 S270B-S268D 0.215836682148677 I229A-V289B 0.215783120549949 Q266D-G269E 0.215317448376296 V235D-C290D 0.214908159833533 C290B-F295B 0.214801415711628 Q266B-M287B 0.214451844950819 V235C-C290C 0.214084386202844 C290D-Q226C 0.213996043005447 Y222A-A288B 0.213553379757028 Y223D-S270D 0.213460151946065 S268C-K276C 0.213198520955515 G269A-T264B 0.212958951458156 Q226E-S267E 0.212426841703909 L233A-L292B 0.212300637279371 S268A-K276A 0.212224951646737 G269A-T265C 0.211358630957788 V260A-S231E 0.210834381168365 T262A-T258C 0.210254930617163 Q226B-C290C 0.209964821915161 Q266D-M287E 0.209844864744791 V235E-T259E 0.209745649755897 T265A-T262C 0.208640600678514 V235A-T259A 0.207996989831193 G269B-T265D 0.207401543636856 F295D-V235C 0.206685080116083 L292A-L233E 0.206646583759343 V294D-L233C 0.206543546412385 T265A-T264C 0.204340498876593 S267A-S273E 0.204064561891875 L232B-T262B 0.204055751536306 I229A-C290B 0.203637747248579 P275B-I283B 0.203411463878283 Y228E-S267E 0.202849597730815 M287A-L233E 0.20272416120731 A288D-P230C 0.202283727317017 M227B-L274C 0.202199400516748 P230A-T264A 0.202029924170346 M227E-V235E 0.202016210201237 L233A-M263B 0.20194003500916 V235C-T259C 0.201775043519446 S231C-V260C 0.201668952213897 S268A-S270D 0.20154448071216 T265A-G269D 0.201417945837296 S273D-K281D 0.200747845076157 A288A-Y228E 0.199798247429445 Q226A-C290B 0.199310060223384 V277D-Y222C 0.199272187172463 C290A-Q226E 0.199127017520892 T262D-T259C 0.198030298269693 Q226A-K276B 0.197772539691768 T259D-T259C 0.197486021519246 V260B-V294B 0.196920443185615 L233D-M287D 0.196736815971271 S231A-A288A 0.196279108377094 Y228D-G269D 0.195745938003941 G269D-S270C 0.19527152770014 T264B-T258C 0.195152794779771 G269A-L274B 0.193297278063888 L232C-T262C 0.193266654294594 Q266D-M287D 0.193172925468915 S268E-K276E 0.192911111332277 S268B-G269E 0.192867928341028 L233A-C290B 0.19282232545621 L274A-M227E 0.192565025686577 S270B-Q266C 0.192560684375605 V260E-V294E 0.19253596987277 Q226A-K281B 0.192345485046292 L233C-M287C 0.192181097379525 V235E-F293E 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