[Wed Aug 24 17:57:02 2016] >Input file: TM4YB9ATD.pdb >TM segments: D27-40_D72-88_D103-117_D127-143_D166-181_D194-209_D282-300_D317-332_D346-361_D375-393_D416-430_D441-454 MAIDEN score = -40.7093 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- C352D-Y383D 10 M285D-H419D 10 M77D-V133D 2.10412880565288 T74D-I129D 1.7136211463602 F349D-C449D 1.64255095991518 L115D-M127D 1.49224709710418 L84D-I137D 1.23767499028401 I328D-I385D 1.15061202063237 Q289D-H419D 1.13636221543065 Y34D-I174D 1.13586884321563 F80D-F424D 1.11280664053264 F30D-F169D 1.10794668007373 F78D-V133D 1.03854531543945 C356D-F445D 1.02702740654841 F30D-I170D 0.999161480769089 S86D-L141D 0.994307020784075 V36D-I174D 0.992692085379215 F80D-W420D 0.965059715089426 M116D-I128D 0.963354226717938 V75D-R132D 0.887841976298328 Q31D-I170D 0.868372568077683 F353D-C449D 0.857122531178506 V75D-Y298D 0.823905521405806 F349D-T452D 0.82201218087265 G287D-C449D 0.817093890950797 M116D-L196D 0.815164679584592 I172D-V326D 0.810257443781803 L290D-I448D 0.805940516170626 Y34D-I170D 0.791026266258253 I282D-V418D 0.777327378818339 F30D-T171D 0.774915844970982 L359D-C379D 0.771728466677482 A388D-P393D 0.77156494982645 G85D-I137D 0.770497219612201 F78D-V135D 0.752101531324115 Q288D-H419D 0.748752003279455 T74D-I128D 0.731237515049927 Q289D-V426D 0.722532163654168 L103D-L207D 0.71823559273175 V283D-T452D 0.715689905972009 S72D-L428D 0.715241390439397 F80D-V133D 0.713680796698546 M285D-V418D 0.710916909391696 C356D-F442D 0.706902902055116 Q288D-V293D 0.703757934367515 N106D-V204D 0.702298992239484 F353D-L450D 0.698665340276703 L168D-V326D 0.695591512537419 L115D-I128D 0.693957666387091 G85D-C138D 0.688340348877356 A295D-F430D 0.681972307137808 A113D-L196D 0.676701835800775 Y34D-L175D 0.672651287173218 G33D-I172D 0.660409883468807 N294D-V426D 0.65384353397337 S72D-G427D 0.652695914787859 I39D-V75D 0.65079718700639 G82D-C138D 0.650491895601592 N35D-A177D 0.645257044576295 S109D-G200D 0.636346897010341 V111D-A131D 0.62697013950608 F83D-G416D 0.626949372965013 G81D-V135D 0.621077921928642 G82D-V133D 0.617854542566476 G33D-F169D 0.615785301568364 F349D-G390D 0.613011677495114 A113D-L198D 0.607400514895085 F349D-P391D 0.594800664178152 F80D-G136D 0.594650775777102 F349D-G386D 0.593143185229168 G33D-I170D 0.585338652010553 N106D-C138D 0.584611049478664 M116D-A131D 0.580863330260925 F108D-C138D 0.580114044705842 Y32D-G173D 0.579281295144811 P79D-F424D 0.574987147926756 C357D-F442D 0.572586843053035 F83D-T417D 0.569720720524696 Y298D-G427D 0.56932042777456 V73D-I129D 0.568415316358251 V324D-I381D 0.567176042851001 S350D-L450D 0.56445618500467 S291D-F445D 0.564032493820172 F30D-I172D 0.550790402458466 F83D-W420D 0.548092317385933 L290D-V426D 0.548079835977616 V351D-H387D 0.547617481706288 F78D-L134D 0.544008571972033 N325D-I381D 0.543803101885577 S28D-S142D 0.539015802778692 F83D-I137D 0.536384953691264 I39D-Y298D 0.535577119211256 Q178D-A319D 0.52511914236027 S29D-S142D 0.523964197159746 S76D-L428D 0.522303758548798 Y32D-I170D 0.519383614011724 L168D-V330D 0.519008562214922 S76D-F424D 0.518131259746325 A38D-L195D 0.512673748887868 L359D-F442D 0.512413631767741 L346D-T453D 0.510945054401258 I174D-Y297D 0.50968182024177 G81D-R132D 0.509108916182356 S29D-I170D 0.507304428233764 F349D-L450D 0.506202251960437 T354D-A446D 0.506030367264265 C356D-Y383D 0.502483385025453 V73D-L428D 0.501428471261346 I110D-A203D 0.495933536352625 T171D-T329D 0.494545308499756 V324D-I385D 0.493779171089217 S86D-S143D 0.493117467754732 F80D-L421D 0.492692810452898 C356D-C379D 0.487317909638485 Q289D-N294D 0.486693521771592 V36D-N294D 0.477965473840595 I328D-L389D 0.467257894685495 F30D-G173D 0.459831132481156 N35D-I174D 0.45934372529634 L290D-N423D 0.451429777910039 S109D-L134D 0.44194749938609 C352D-H387D 0.441027626622756 T360D-V443D 0.431208590965117 A355D-V380D 0.429980692019924 C352D-V384D 0.427704032144979 P79D-L421D 0.425740751116291 F83D-L421D 0.425633783033369 L290D-F445D 0.425035044412952 M77D-I129D 0.422424531835407 V358D-S376D 0.420125369635227 F353D-F445D 0.418899937843046 Q178D-T318D 0.418702385957033 V351D-S382D 0.414310927943785 Y34D-I172D 0.414260337996812 C356D-V443D 0.413438394802981 M116D-I129D 0.407973040085499 F180D-L195D 0.407347802873539 N40D-Y298D 0.40390299117945 S29D-S143D 0.400684925314145 S76D-T425D 0.399502664664792 F30D-I174D 0.399005493600277 G27D-F169D 0.397922286398038 S291D-H419D 0.397413045797183 T360D-S441D 0.396738908146585 A37D-I174D 0.39504056395092 I328D-A388D 0.393505320931166 L290D-S422D 0.392158694807706 L359D-V380D 0.391320863949372 A113D-V135D 0.387410008425662 A38D-Y298D 0.379027876344313 A139D-A203D 0.378096305104161 T321D-I381D 0.374711477226881 G27D-Q206D 0.373328870353647 V324D-V384D 0.370640737682027 F80D-I137D 0.370392468646708 T360D-F442D 0.369844968201811 I107D-L207D 0.367005966553608 F78D-F424D 0.363886570648973 G82D-W420D 0.362800191508801 A177D-A323D 0.362761696678735 Q289D-Y383D 0.362605249721833 A38D-L196D 0.361894127819001 M88D-L141D 0.361511537751209 L346D-G390D 0.360861250297202 P79D-S143D 0.360359697859436 P112D-L134D 0.359520979702936 Y32D-G85D 0.359446516990295 Q288D-H387D 0.359372406006324 F349D-C379D 0.358337606248649 C352D-A446D 0.358276429288942 L175D-V326D 0.357361349582018 C352D-T360D 0.355769380415582 Q31D-P166D 0.352507313236676 Y32D-V75D 0.35134973016733 N40D-Y297D 0.350018281516079 N105D-P202D 0.348959038933827 C357D-V443D 0.347084027604138 M77D-G136D 0.34670796334197 N105D-C138D 0.346476347596738 S86D-S142D 0.343383754965151 S28D-A139D 0.342731592931018 Y298D-V380D 0.341763854732596 N325D-P393D 0.340659088183989 S29D-A139D 0.340151701471282 T171D-G322D 0.339067094431758 S29D-Q167D 0.336789644236111 Q178D-G322D 0.336751463621312 S76D-V426D 0.333364691913882 F353D-V447D 0.330146136926295 V75D-L428D 0.329999730664463 L175D-A319D 0.328615751833307 M285D-V454D 0.327317452479027 S28D-A203D 0.326541134944869 S291D-I448D 0.326404540706561 Y299D-G322D 0.326289366039614 A38D-Q178D 0.326034710795226 N294D-P391D 0.325685714271877 Q167D-P393D 0.325644963937557 N294D-H419D 0.324640506556722 L115D-A131D 0.324370220431062 G173D-A319D 0.322850170158835 A332D-G348D 0.322560685709227 S291D-A446D 0.322008802601308 L359D-V375D 0.321545636347053 Y298D-V384D 0.32003680699867 A286D-S392D 0.319822202313297 S291D-A355D 0.318820294715049 V358D-C379D 0.318413174280004 S29D-F169D 0.315127610950998 P79D-Q289D 0.312310847217191 F78D-I137D 0.312065083114888 N106D-Q206D 0.311753284388287 G292D-G386D 0.311642338577502 N35D-V135D 0.310367982317608 G81D-G416D 0.310207267331239 F353D-I448D 0.308562770542101 C352D-V454D 0.308468481032983 S350D-C449D 0.307404771930685 L284D-T452D 0.306534136024511 Y34D-P79D 0.303719578367638 C331D-H387D 0.302879344960608 M77D-F424D 0.302525481833147 T171D-C331D 0.302454251745184 P79D-V133D 0.302146189797066 G361D-A446D 0.301680002980729 A177D-L195D 0.301548652555341 T329D-L389D 0.301215528708937 Y34D-P166D 0.300725159191529 M116D-M127D 0.299903800986482 G81D-G427D 0.2993189314153 S392D-H419D 0.299024025651842 G181D-L195D 0.298540574844779 I39D-L196D 0.298429564854491 C331D-V384D 0.297759936220984 Y34D-V326D 0.297694216781477 Y32D-P112D 0.29743184224992 V384D-C449D 0.29736665899101 M285D-N294D 0.296608324199094 A177D-G197D 0.29615574523657 S109D-A203D 0.29531677249021 L284D-P391D 0.294689897820977 S72D-V429D 0.294519975259007 V176D-G181D 0.293883561701071 I129D-C138D 0.293840841433618 L87D-L141D 0.293672114528995 V283D-Q288D 0.293113190552557 S291D-G386D 0.292576952647751 I110D-V204D 0.291888808927835 A38D-A300D 0.291885544407549 I107D-V204D 0.290783084960675 A37D-L175D 0.289107066921443 G322D-H387D 0.2877323923273 T321D-V384D 0.286791362097556 G292D-S392D 0.285920837289651 N35D-G82D 0.284041470109498 P166D-S392D 0.283964464801467 T74D-P112D 0.283670023378868 V130D-C138D 0.283636649942328 N294D-G427D 0.280055245635096 G27D-L207D 0.279436057127607 Y34D-Q178D 0.278786112616072 L347D-G390D 0.278738084938795 V351D-V384D 0.277671275243673 I385D-C449D 0.276952033056948 G140D-N423D 0.275436139764199 Q167D-T329D 0.275409023971013 P112D-M127D 0.273271666979253 G292D-L428D 0.27280075367003 G27D-A139D 0.27008311827395 S143D-H419D 0.268848332072923 Y34D-R132D 0.267907067200724 L347D-G386D 0.267265442079405 F78D-R132D 0.266403474783298 G27D-N106D 0.264679724176869 N294D-S392D 0.263284233815479 N106D-P202D 0.263008458397004 G33D-N325D 0.262396081229213 G173D-G200D 0.262210071182208 C379D-V443D 0.260922624371684 Y34D-V75D 0.25998310162465 V75D-V133D 0.259762887952967 V73D-Y299D 0.259439439863307 G348D-L389D 0.258831594278555 I172D-G322D 0.258760146017062 V326D-Y383D 0.258320831285047 F80D-N423D 0.257679440578792 N35D-G140D 0.257523932646426 G85D-H419D 0.256693896158488 G173D-L198D 0.256191884835674 Q31D-P79D 0.255908017867442 L114D-G200D 0.255716398997043 F83D-G140D 0.253632947859431 F30D-G200D 0.252630639234902 I39D-R132D 0.252321970010506 Q167D-Q288D 0.251385663316609 V293D-H387D 0.250214593288733 Y32D-S109D 0.249763505060129 G136D-N423D 0.249351229207803 G82D-I137D 0.248481358207007 N105D-I137D 0.24767307607596 G33D-S143D 0.246155757629168 Q288D-N294D 0.244970525923127 Q288D-T425D 0.243640085432113 N35D-G181D 0.242607252045023 Y32D-Q178D 0.242371437275205 G117D-L196D 0.24053958545556 L115D-G197D 0.239571983836475 N294D-Y299D 0.239194125761998 Q289D-F430D 0.238605544151603 S76D-N294D 0.238597697284238 P112D-L198D 0.238275688532309 Q288D-S350D 0.237974416325283 Q31D-F78D 0.236498851449345 G292D-F424D 0.236065577654141 I172D-A323D 0.235578126463631 T318D-C379D 0.234766088184568 T171D-T318D 0.234352926580078 A177D-Y298D 0.233938775696642 F108D-G136D 0.233039596901948 P79D-Y297D 0.232244551541672 M116D-G197D 0.231440751172927 G320D-A378D 0.230457540469652 L284D-P393D 0.230439776344284 L114D-P202D 0.230279632720731 S291D-C449D 0.229773760156879 Y297D-V326D 0.229728752119344 N106D-G140D 0.228801527597271 S86D-S422D 0.228623735935886 V293D-I381D 0.227857940878437 S28D-S86D 0.227842536209542 G292D-F349D 0.227594260091063 F349D-H387D 0.227190536771301 S382D-S392D 0.227078097265776 S143D-S392D 0.226044238378187 A300D-G320D 0.225557901459593 G181D-G197D 0.224935247447724 V111D-I128D 0.22487218179117 Y34D-V176D 0.223027072028071 Y297D-H387D 0.222798439441499 I179D-A319D 0.222737825735217 G181D-G320D 0.222685895641346 L103D-Q206D 0.221852364325436 S109D-V135D 0.221256601445075 S76D-Y299D 0.221221376253051 L84D-G140D 0.220457506472397 G348D-L450D 0.22028010424729 F78D-Y297D 0.220026920694464 V293D-V384D 0.219517694012203 A295D-G427D 0.219240511322216 H387D-T453D 0.219222551619749 C379D-C449D 0.219144196154912 P79D-P112D 0.218833708759751 C138D-T199D 0.218800186390431 T171D-H387D 0.218582203491302 G287D-C352D 0.218210568706716 A355D-C379D 0.217827093433105 G33D-V326D 0.217527415194841 M116D-I194D 0.217481497622519 N294D-N423D 0.216022122776827 N294D-G322D 0.215738061687819 S291D-G427D 0.214328013382363 G287D-V426D 0.214086248845591 P166D-T171D 0.214041734627104 P79D-L290D 0.212669429812109 S86D-I137D 0.212444728104592 G27D-F209D 0.212369883849311 V429D-I444D 0.212106891118217 Y34D-T199D 0.211783945419185 Q167D-C331D 0.211714071139842 F80D-V426D 0.211632077978799 G81D-L134D 0.211596549896535 S72D-S422D 0.211487980101475 G81D-T425D 0.211068366137775 P79D-T425D 0.211043061667597 L284D-G416D 0.210671271341877 T321D-V380D 0.210586772786618 A37D-G427D 0.210275861616163 P112D-L195D 0.209883697677163 V283D-L451D 0.209882612618164 V133D-F424D 0.209594931268956 Q178D-T199D 0.209358995568869 S291D-V351D 0.208859763939063 T74D-L134D 0.208744406079147 G81D-I137D 0.208307921728344 S76D-V130D 0.208197020531717 V73D-F430D 0.207876271614357 G27D-G200D 0.20776162302101 T321D-G386D 0.20750504460344 Y32D-N106D 0.207056636226791 L175D-V324D 0.206948721199261 G386D-T453D 0.206875488554444 L290D-P391D 0.206758420928807 G82D-L141D 0.206023429814902 N40D-G427D 0.20584857472865 L347D-T452D 0.205767787111204 S109D-S143D 0.205213303810653 A286D-F445D 0.205010674866647 G320D-V384D 0.204827254872799 F83D-M88D 0.20425945791614 F80D-V418D 0.20389075773464 G140D-P166D 0.203566668312104 Y383D-A446D 0.203513185818494 N35D-L196D 0.203255092749696 Y299D-V317D 0.203128592533065 I39D-G427D 0.202937766666855 P79D-L87D 0.202427874326521 P391D-L450D 0.201045533866446 N105D-L141D 0.200564962784478 G136D-G200D 0.20015064420015 I172D-T318D 0.200124311383384 S29D-S392D 0.199078914647736 C138D-V204D 0.198670440860649 C352D-I444D 0.198575614944268 T321D-H387D 0.198301322171454 P393D-G416D 0.197050934337073 G33D-G82D 0.196865026231348 L284D-N423D 0.196785160295572 Y32D-N294D 0.196125708191939 I296D-C352D 0.194737599213329 V293D-F349D 0.194651635238099 P112D-V130D 0.194585537684723 N106D-L114D 0.194464588956978 S441D-V447D 0.193467078699939 I170D-V330D 0.192821298895403 F108D-A139D 0.192593639802134 G33D-I39D 0.192364870514276 I179D-G322D 0.192177188156564 T329D-P393D 0.191760773457773 I296D-C356D 0.191204047851509 G82D-S422D 0.19103904611387 S29D-S86D 0.19036703402984 G117D-I128D 0.190061249446405 A295D-T318D 0.189513542726549 G287D-V454D 0.189442239170694 A300D-G427D 0.189360198727405 A295D-A300D 0.18888586480093 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