[Wed Aug 24 17:33:16 2016] >Input file: TM5AWWATD.pdb >TM segments: Y17-32_Y71-90_Y113-128_Y158-176_Y179-197_Y218-234_Y270-287_Y309-325_Y369-385_Y394-411_E27-52_G10-26_G51-66 MAIDEN score = -132.5535 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I320Y-A372Y 2.29421486062126 I120Y-I165Y 1.21389237809431 I77Y-I165Y 1.21308695976468 F73Y-I165Y 1.20113831995673 I320Y-L373Y 1.15597411455045 Q26G-L52G 1.0651320922778 I34E-V405Y 1.02197540466462 V271Y-I403Y 1.00114149460964 V271Y-D410Y 0.995210894586775 V23G-W170Y 0.983908001501416 F41E-V193Y 0.980778243905851 Y80Y-T115Y 0.95369746335729 A278Y-E315Y 0.950492880838886 I175Y-I180Y 0.94160521824165 I320Y-L375Y 0.929963542883254 V15G-F63G 0.859573953841474 I77Y-L168Y 0.855527527058422 Y80Y-I120Y 0.849877812060153 V18Y-I180Y 0.847022238146806 T176Y-N182Y 0.839271812295662 I77Y-L169Y 0.835714115199378 F125Y-V158Y 0.821539897973105 I120Y-V162Y 0.821530538406622 I34E-L409Y 0.811103920240128 A277Y-E315Y 0.80454378276139 I274Y-V319Y 0.795976727685887 D49E-L30Y 0.780680248935655 F189Y-V407Y 0.770398307186937 W196Y-F396Y 0.767431648746506 A124Y-V162Y 0.742272255235971 F228Y-V405Y 0.740720139101154 F396Y-L401Y 0.737602717716968 L24G-I117Y 0.736455384561694 M42E-V194Y 0.732390862485941 V319Y-L375Y 0.726536343765142 M42E-V193Y 0.726206200343964 L19G-L59G 0.720797286538144 W196Y-A395Y 0.712737132789303 L186Y-V407Y 0.712079984761117 I274Y-I403Y 0.710167949140894 G31Y-I72Y 0.686928328698509 Y28Y-L168Y 0.68690736167158 L22G-L52G 0.680225073445357 I274Y-T323Y 0.676948816791437 F220Y-I385Y 0.669792803963716 A277Y-F322Y 0.667043047494955 L19G-A166Y 0.665584987985218 V234Y-G371Y 0.665329111900475 Y80Y-L169Y 0.664884008479372 Y80Y-L122Y 0.662498376474077 P79Y-Q126Y 0.655353252928872 I192Y-G400Y 0.653775613676856 A83Y-L122Y 0.651750905708165 I34E-F228Y 0.650853933998337 F128Y-V158Y 0.649563506360331 P79Y-L122Y 0.647869238477409 A172Y-N182Y 0.646268722594594 M78Y-A279Y 0.637121746329762 M42E-L197Y 0.636903563452858 F189Y-V404Y 0.635312435481576 S185Y-I272Y 0.634845588668017 G31Y-F73Y 0.625181637650366 L45E-V194Y 0.622983867966941 A278Y-V319Y 0.622473306367071 A172Y-I187Y 0.620328946276732 T82Y-F276Y 0.617394025197125 L321Y-A372Y 0.612287159053372 F37E-A408Y 0.610592434823321 L25Y-I187Y 0.605534223749337 A16G-F63G 0.59896851006584 G76Y-I165Y 0.583113487091348 V15G-L59G 0.571162073404489 G31Y-S71Y 0.569999429101716 R29Y-I187Y 0.569272586462529 A83Y-R116Y 0.564152219844061 E195Y-A395Y 0.560689112384319 A32Y-I72Y 0.556776849676298 V24Y-I175Y 0.554263292740058 P273Y-F322Y 0.546809039088247 L24G-Q113Y 0.542547979229013 F313Y-L380Y 0.541640397081676 F73Y-G164Y 0.539318688145834 M42E-W196Y 0.536612292928046 I85Y-F276Y 0.535649898246305 L312Y-Q383Y 0.534936406767583 L25Y-A190Y 0.534296444749914 T38E-V404Y 0.534097535214338 I274Y-F322Y 0.529855615951015 R116Y-L169Y 0.523785244893507 A227Y-V378Y 0.52304834178499 I274Y-L402Y 0.516743481557594 F41E-A190Y 0.511122000066935 Y12G-F63G 0.50474796015838 L45E-R29Y 0.501668440029787 V316Y-L380Y 0.498245195927489 L13G-V159Y 0.49797948206259 F63G-A163Y 0.495886061799419 I81Y-F276Y 0.490767831562828 A16G-T160Y 0.489741899844433 A32Y-F73Y 0.489537613113867 L312Y-L380Y 0.487330261403289 G127Y-V162Y 0.485086205708853 V271Y-V407Y 0.479865149520464 Y48E-L30Y 0.475497574131745 V23G-L167Y 0.4748857445962 I77Y-T184Y 0.474619699984373 P273Y-E315Y 0.474103716467004 I180Y-T411Y 0.474074291692972 P284Y-E315Y 0.473406197955984 S185Y-I403Y 0.471342152465059 S185Y-V271Y 0.47058290395475 Y48E-R29Y 0.464533862281774 V27E-A233Y 0.459287648709683 Q26G-Y114Y 0.459002472388486 Y80Y-G118Y 0.45666113088566 P79Y-I120Y 0.454845009290333 I175Y-G181Y 0.453270986304877 V319Y-T379Y 0.450792906066837 V24Y-R174Y 0.433883619273795 V405Y-D410Y 0.429034661648592 A16G-V162Y 0.426098841314634 F223Y-I385Y 0.423897014932729 F189Y-I403Y 0.418985996720156 T38E-V193Y 0.416671396581811 L22G-T56G 0.414591988934895 T31E-A229Y 0.409907408318373 V28E-A229Y 0.408599453300195 D49E-V194Y 0.407343790490883 F52E-L30Y 0.404930073712521 V15G-A163Y 0.404667657212151 V20G-L167Y 0.400742207315659 Y12G-V159Y 0.395970917889601 F73Y-L168Y 0.395839492093157 V378Y-L401Y 0.39529102543522 I320Y-F374Y 0.393760476603664 A124Y-V159Y 0.392916882962459 G123Y-I165Y 0.39020418792057 I72Y-I165Y 0.389303389456261 A121Y-V162Y 0.387865493040689 V319Y-A372Y 0.387476600496844 I275Y-I403Y 0.38696665944617 I77Y-I188Y 0.386604375245179 V23G-L169Y 0.386237591455068 V193Y-A395Y 0.386058929549526 L45E-A26Y 0.385718294819114 I34E-A408Y 0.384270158086174 M171Y-I187Y 0.381972614996639 I77Y-I187Y 0.377224922165997 F276Y-F322Y 0.376524174743934 I192Y-A395Y 0.376402314413332 I274Y-I399Y 0.37254226384999 I120Y-A166Y 0.371730101347659 L169Y-T184Y 0.370822752738311 T31E-F228Y 0.368048580574689 F41E-V194Y 0.366783230410579 M78Y-I275Y 0.365543338392945 L381Y-G398Y 0.363504546541829 L45E-A190Y 0.362414678202027 F20Y-I175Y 0.361768101953411 A74Y-A124Y 0.360887697135643 P79Y-I165Y 0.359763010323284 T38E-A408Y 0.359256382313499 T323Y-L375Y 0.359081327783541 Y21G-G60G 0.355608647164091 G181Y-L186Y 0.354758595927087 G127Y-V158Y 0.354538455745055 Y12G-L66G 0.354166343168626 L19G-L167Y 0.353359784325177 T56G-L167Y 0.353344443882701 L317Y-G376Y 0.350783269379451 L19G-A163Y 0.350739865680095 A190Y-A395Y 0.349627355475232 T82Y-G119Y 0.346938889255532 L24G-R116Y 0.343404645306496 Y80Y-I165Y 0.343036143698755 V15G-V62G 0.341797017706225 V24Y-R29Y 0.341022774093023 F20Y-E173Y 0.339934922759315 P79Y-Q282Y 0.338516009290064 V271Y-L402Y 0.337073451547493 A27Y-A172Y 0.336635147055774 V316Y-L377Y 0.336390725642271 T379Y-S397Y 0.3323925032129 V43E-L197Y 0.326658162102902 T38E-E195Y 0.325706509359427 L35E-F228Y 0.323925869265315 M171Y-G183Y 0.321963627203084 R29Y-G191Y 0.3215543084676 M231Y-L375Y 0.32031117281363 L45E-V193Y 0.317940987927807 Q126Y-V158Y 0.316957147284427 Y21G-V159Y 0.315790803727928 R54G-L59G 0.315781907476133 I81Y-G119Y 0.314436042969906 G76Y-G183Y 0.314121192263615 I394Y-G400Y 0.314023296837753 G46E-L197Y 0.312925975483141 Q113Y-E173Y 0.312889434474251 A227Y-L401Y 0.310458723780294 A74Y-A163Y 0.310100540735713 I165Y-W170Y 0.309755270012349 G119Y-A163Y 0.309305645845462 T82Y-T115Y 0.30820107656905 T21Y-E173Y 0.30818859771332 A232Y-V405Y 0.306303057383655 T50E-E195Y 0.304237733937608 P382Y-F396Y 0.303527874742062 Y324Y-L369Y 0.302441623634683 G270Y-G371Y 0.301604702906992 I192Y-V404Y 0.299155750373662 Y21G-G164Y 0.29889677243849 Y28Y-I187Y 0.298502315706111 A172Y-L186Y 0.298025417578594 P79Y-Y114Y 0.297956243711244 G230Y-G376Y 0.297551696583738 L24G-A166Y 0.297469457099732 V378Y-L402Y 0.296799233906343 P273Y-T379Y 0.296137093569997 T379Y-L402Y 0.294945727671568 L169Y-R174Y 0.294510857917829 E315Y-P382Y 0.294502475396557 V23G-T56G 0.29442790505604 A227Y-G406Y 0.294288617856207 A277Y-G376Y 0.293273162960237 Y80Y-Q282Y 0.293034175261456 A278Y-L318Y 0.292330229942981 A232Y-L409Y 0.291716874226831 Q161Y-A166Y 0.291126998562791 L375Y-L402Y 0.288216342804136 L59G-G164Y 0.287356724055311 Y53G-V24Y 0.285685253746198 R116Y-M171Y 0.285188860996222 V20G-Y80Y 0.285138577312864 S71Y-G127Y 0.283800975421229 A33E-L409Y 0.283777277236116 L22Y-G183Y 0.283726493720482 E315Y-T323Y 0.283136232192761 E29E-F37E 0.282754918631057 A227Y-G400Y 0.282136059057438 A395Y-L401Y 0.281791354780534 I81Y-R116Y 0.281678639942143 E315Y-Q383Y 0.279923320033398 G311Y-G398Y 0.279727653101459 G230Y-L402Y 0.278761728060788 M231Y-W370Y 0.277518502208862 P79Y-P284Y 0.275647896406918 I165Y-M171Y 0.275127604914395 T82Y-I280Y 0.274258535469557 I188Y-I403Y 0.274231500972985 T82Y-L122Y 0.273878166638795 M231Y-V405Y 0.272748517668355 A64G-A27Y 0.27172966361284 G376Y-G400Y 0.270256153315679 R17Y-L22Y 0.270011894200393 I81Y-I275Y 0.270009774483631 T184Y-P273Y 0.268753495538703 G230Y-L401Y 0.267266132376689 G270Y-V407Y 0.265742653875542 G17G-A124Y 0.264296330909422 R29Y-V194Y 0.262168884259311 F219Y-I385Y 0.260573718207649 S71Y-A163Y 0.257599534581086 A40E-A190Y 0.257551300428664 L75Y-Q161Y 0.253961738584092 G230Y-G398Y 0.253518383507628 L380Y-G398Y 0.253252109983888 V23G-Y114Y 0.252485090732167 M42E-R29Y 0.250643625789499 Q32E-G230Y 0.250609774208523 E195Y-G400Y 0.250034204552552 G14G-G60G 0.248618212123906 L221Y-P382Y 0.246356817708929 L380Y-G400Y 0.245318157116216 V20G-Y53G 0.244718350132124 V23G-Y80Y 0.243532912091511 T21Y-T176Y 0.242837239872808 A16G-A124Y 0.242542303037434 T38E-L186Y 0.242196460615433 F228Y-G398Y 0.241965659735096 Q126Y-G164Y 0.241368323397169 L19G-T160Y 0.239747589916361 G270Y-F276Y 0.23945120246847 V378Y-I399Y 0.239319144619643 A64G-I72Y 0.239295288171824 G270Y-F322Y 0.239226098641378 I175Y-N182Y 0.238310027006975 L24G-T56G 0.237686168803287 V193Y-V404Y 0.237621509034555 G14G-L66G 0.237125518275153 R29Y-M171Y 0.235194501392643 L75Y-Q126Y 0.235160772795302 G31Y-Q161Y 0.235090971531766 V271Y-G406Y 0.234956312200688 A16G-A163Y 0.234664273573669 M78Y-R116Y 0.234282571659177 G119Y-W170Y 0.234066541162679 F37E-A190Y 0.233916069071522 Q88Y-G118Y 0.233189161577718 S185Y-V407Y 0.233145426511094 V316Y-G376Y 0.229781028116918 N182Y-G406Y 0.229736381946586 Q26G-Y53G 0.229502701360128 T50E-L197Y 0.228614796760742 F223Y-G398Y 0.227378153212849 G17G-A163Y 0.227075650278146 G123Y-V158Y 0.22651086859794 Y21G-Q26G 0.226154994975488 Y28Y-V193Y 0.225977184610192 F63G-A166Y 0.224882091263335 Y80Y-A124Y 0.224368905188059 L281Y-I314Y 0.224362173510184 A277Y-L318Y 0.222295259580076 Y48E-A190Y 0.221847515982835 A172Y-T184Y 0.221293214005307 G76Y-V162Y 0.221148546282537 Q88Y-F322Y 0.221094744793801 G17G-V158Y 0.221093935561338 I192Y-V405Y 0.220417889419082 G376Y-Q383Y 0.220156336765687 I224Y-G400Y 0.219882906096862 V24Y-I180Y 0.218885297824048 V319Y-I403Y 0.21701176679604 G230Y-G406Y 0.216290410909076 A27Y-S71Y 0.215150364969128 T56G-L168Y 0.21497268724429 A84Y-R116Y 0.214746878912723 I34E-A232Y 0.213454202796909 I224Y-V404Y 0.212979935671941 F37E-G406Y 0.211080373233965 V25G-L52G 0.211034194668406 Q32E-M231Y 0.210765491478397 G76Y-L168Y 0.209890333357394 I77Y-V162Y 0.209732521643865 F228Y-L401Y 0.209728608568568 Q88Y-P273Y 0.209603495683117 G76Y-S185Y 0.20809752544161 G17G-L167Y 0.207955740491522 R54G-M171Y 0.20774638118113 V61G-M171Y 0.207634141469712 I188Y-G406Y 0.207618220193997 L19G-V162Y 0.207459071453897 G118Y-V162Y 0.207205432918033 V20G-V162Y 0.207193123337343 T184Y-G406Y 0.207056077094604 G119Y-T184Y 0.206986884506422 T82Y-I285Y 0.206458053067317 I188Y-T411Y 0.206238293061729 F11G-A65G 0.205740528908045 L19G-G60G 0.203975029556337 F73Y-V158Y 0.203798194049028 I280Y-T323Y 0.201714001328985 L35E-A40E 0.201469977784924 E173Y-G183Y 0.200654670770654 T82Y-I188Y 0.200654502377768 P273Y-V319Y 0.200391532043228 V378Y-F396Y 0.200269292314312 I120Y-G164Y 0.200149780061325 F73Y-M78Y 0.200010537990969 V61G-G164Y 0.199489489425438 S185Y-I275Y 0.199289102442629 I77Y-G123Y 0.199124518229215 R17Y-N182Y 0.19608931541259 A27Y-A190Y 0.195331851183591 G230Y-T379Y 0.194013772820441 T379Y-G406Y 0.193413010736649 M231Y-L380Y 0.192630889269267 T31E-A222Y 0.191668384182675 G76Y-T160Y 0.19165953204328 V20G-G119Y 0.190567590311608 V234Y-L375Y 0.190343562295625 A40E-T21Y 0.190112951604531 V24Y-M171Y 0.189778392295106 I86Y-G118Y 0.189752747392872 D49E-A27Y 0.18899611582181 R174Y-G179Y 0.188772222651239 A32Y-T160Y 0.188380163258457 T176Y-I188Y 0.188344784351927 F73Y-V159Y 0.187896160118359 G123Y-L167Y 0.18786144511196 R116Y-A172Y 0.187415973552373 Y80Y-G119Y 0.187410891608145 S310Y-Q383Y 0.187252358738966 A166Y-T184Y 0.186881946164927 T38E-I187Y 0.186518101816927 Y48E-A32Y 0.18636355726452 G60G-L167Y 0.186165196378848 A278Y-G311Y 0.185589703687405 I72Y-A163Y 0.184505897027416 G76Y-I188Y 0.184401710274669 I274Y-V378Y 0.183500461197251 G76Y-I192Y 0.183198351673842 F223Y-P382Y 0.182555395835354 V271Y-V404Y 0.182262507509142 A26Y-G191Y 0.182009032885594 F189Y-T411Y 0.181845446714939 L19G-T56G 0.180875097030909 M231Y-G400Y 0.180117645158816 R29Y-V193Y 0.179562876277488 G371Y-L402Y 0.179312169849371 L317Y-G398Y 0.179141431267288 M231Y-S397Y 0.179127941136062 T184Y-A408Y 0.179052231756813 F20Y-G183Y 0.178242710242133 L167Y-G183Y 0.178165408897951 G46E-Y28Y 0.178004624577152 M42E-V18Y 0.177723577591498 L221Y-S397Y 0.177503231093306 D49E-A26Y 0.177433312495137 D49E-R29Y 0.177192941185586 T50E-A32Y 0.177160401693661 Y21G-G119Y 0.176735627248453 T115Y-T184Y 0.17567654645274 A233Y-G371Y 0.175416287585637 F73Y-A124Y 0.175315377615432 S185Y-A278Y 0.175178054974946 L281Y-L318Y 0.174926663431711 T82Y-A124Y 0.174866499887471 V15G-L66G 0.174506858481644 F11G-V159Y 0.173735856243419 I85Y-A278Y 0.173731259745018 Y80Y-G127Y 0.173506305449212 A83Y-I280Y 0.173179970937461 A190Y-G400Y 0.173158773053825 L186Y-G400Y 0.172940231664031 L30Y-G164Y 0.172653844888376 I72Y-G164Y 0.172647872667803 L13G-G60G 0.171806694247025 G60G-Q161Y 0.171357810806265 G76Y-A190Y 0.171221268778975 M42E-V405Y 0.170980785390672 F374Y-G398Y 0.170974845205588 I72Y-T160Y 0.170904641181554 D49E-G31Y 0.169349388035378 Y21G-G118Y 0.168933562121565 L35E-G230Y 0.168887005163929 G118Y-Q126Y 0.1687187816677 M171Y-G181Y 0.168518178923081 Y28Y-R174Y 0.168287292127639 A27Y-I187Y 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