[Wed Aug 24 17:34:09 2016] >Input file: TM5AWWCGD.pdb >TM segments: Y18-35_Y69-101_Y107-127_Y157-176_Y179-205_Y214-235_Y266-283_Y311-330_Y364-386_Y391-411_E454-483_G491-512_G534-553 MAIDEN score = -77.0508 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- BBeads pair Empirical energy ----------- ---------------- Q386Y-I391Y 10 I320Y-A372Y 2.48509070277138 I175Y-I180Y 1.72509967121582 L266Y-T368Y 1.26646656589328 V91Y-L97Y 1.19940262885361 I34Y-S71Y 1.18042320957254 I165Y-W170Y 1.13628344105279 I34Y-I72Y 1.13389772441176 I385Y-I391Y 1.09474805499708 R69Y-A74Y 1.06221843162229 Y496G-F547G 1.0277194753644 I120Y-I165Y 1.00537864019519 V18Y-I180Y 0.993697560011151 V499G-F547G 0.978426317140419 I274Y-V319Y 0.910291242664124 A83Y-R116Y 0.905739664373979 W196Y-A395Y 0.885447260220935 V405Y-D410Y 0.871730650599656 L266Y-L367Y 0.8708907756364 L508G-Q113Y 0.868505926932229 I34Y-R69Y 0.865637541205505 I320Y-L373Y 0.828916230746635 I120Y-V162Y 0.820183035631003 F465E-V193Y 0.806310634087345 A269Y-D410Y 0.773052788453774 P512G-R109Y 0.767385056597229 V499G-L543G 0.739969625367239 F189Y-V404Y 0.7276426151586 I320Y-L375Y 0.723251433867822 L90Y-L97Y 0.721019013886612 G76Y-I165Y 0.713907423177622 T327Y-T368Y 0.712013080476256 V271Y-I403Y 0.711507032121652 G31Y-I72Y 0.681483946422654 L321Y-A372Y 0.6741261750491 F189Y-I403Y 0.670147123483247 I180Y-T411Y 0.66956690797062 Y80Y-L122Y 0.668009506139505 F73Y-I165Y 0.658992044422239 Y496G-L550G 0.650648992052779 I77Y-T184Y 0.650123158610175 Y324Y-L369Y 0.644176894449182 T21Y-I180Y 0.634479712516268 A328Y-V364Y 0.632500157409019 V546G-A551G 0.630267361288036 A124Y-V157Y 0.629159794826478 V158Y-A163Y 0.628409669163366 E195Y-A395Y 0.62194550740576 Q386Y-H392Y 0.620244427747908 G31Y-F73Y 0.619420345290473 L169Y-R174Y 0.617122615334002 L197Y-R202Y 0.613951247870348 I320Y-G376Y 0.609938655909154 R538G-L543G 0.603391367765223 A277Y-F322Y 0.602689148408457 F495G-A500G 0.598624561674221 V319Y-L375Y 0.5862624955419 F465E-A190Y 0.584698351879596 P79Y-G123Y 0.582452671843881 Y80Y-I120Y 0.576174618097617 M466E-V193Y 0.575770009762753 F228Y-V405Y 0.574676387370326 I89Y-V94Y 0.574551896265456 Y472E-A26Y 0.573782334623925 V507G-W170Y 0.572876081996178 I458E-V405Y 0.566263402939859 G470E-V194Y 0.560079267091856 I110Y-R116Y 0.55836725124347 L469E-V194Y 0.556945419837927 V234Y-N267Y 0.551937840304535 S185Y-I403Y 0.547800604851892 A121Y-V162Y 0.545422520647309 G31Y-S71Y 0.545402201177778 P35Y-F70Y 0.543775069253854 P273Y-F322Y 0.542207335588581 M466E-L197Y 0.541770020266921 L266Y-W370Y 0.537391127955532 N112Y-I117Y 0.536445405535112 N182Y-I187Y 0.535318889358094 V499G-A163Y 0.529242180879725 F125Y-V158Y 0.523807563585724 A227Y-V378Y 0.521860475665159 T455E-F228Y 0.520635093886104 G123Y-V158Y 0.519577271546662 F461E-A408Y 0.516342490045053 F20Y-G179Y 0.515270481032455 R116Y-I165Y 0.511361721767949 P382Y-F396Y 0.510403646852132 L90Y-P95Y 0.509598887436712 A500G-V162Y 0.509219723377802 A32Y-F73Y 0.509124947242099 F465E-V194Y 0.507125020876476 F217Y-A222Y 0.507041116404061 R477E-L482E 0.506004787777982 A277Y-E315Y 0.505643082572037 A268Y-T323Y 0.504707803571214 F33Y-R69Y 0.497402007507575 Y496G-V159Y 0.493196220645074 P382Y-G398Y 0.492473316981439 L552G-P35Y 0.492236672162636 D473E-F478E 0.492226069580263 L471E-F476E 0.491985161871533 L281Y-E315Y 0.490829735358222 A32Y-F70Y 0.489529196388748 V24Y-R29Y 0.48671256444676 A83Y-Y114Y 0.48614372654299 A500G-T160Y 0.482720689396746 I77Y-L168Y 0.462100659041413 P79Y-I120Y 0.458392445884143 E173Y-G183Y 0.453716244153421 Q235Y-L266Y 0.452424165391355 I192Y-G400Y 0.451829379190642 G119Y-I165Y 0.447745660082245 T82Y-G119Y 0.44632166048264 L503G-A166Y 0.446156077316539 T462E-E195Y 0.443046117913218 I81Y-G119Y 0.438897632408139 M87Y-I111Y 0.438472285803621 E315Y-I320Y 0.434545718396462 F33Y-S71Y 0.434211994812531 V504G-A163Y 0.430080136357277 F396Y-L401Y 0.427278091513564 G123Y-I165Y 0.42476225575801 V193Y-A395Y 0.423797888110569 I72Y-A163Y 0.423228827880209 Y80Y-Q282Y 0.42246877559 L508G-R116Y 0.42168860065738 L100Y-R109Y 0.420340766608041 M78Y-I165Y 0.419077652848697 A172Y-N182Y 0.418093523529758 F465E-G470E 0.41564793576366 I34Y-F73Y 0.414638536887216 A278Y-E315Y 0.412852348185545 L497G-V159Y 0.410515503604262 L469E-V193Y 0.410477487040652 V316Y-T379Y 0.410343125348545 L503G-A163Y 0.409546909988003 Q126Y-V158Y 0.406170557208292 M78Y-I275Y 0.405332209308613 V378Y-L401Y 0.404411029086887 I77Y-I165Y 0.403522692365273 F219Y-I385Y 0.401722109907687 T379Y-S397Y 0.400467523204576 L186Y-V407Y 0.398789698710917 I165Y-M171Y 0.398508707794628 I320Y-G371Y 0.398289924212484 L402Y-V407Y 0.392982026213054 V319Y-Y324Y 0.392686033444013 L506G-L536G 0.392399302270407 I72Y-I165Y 0.392380145785483 P273Y-L318Y 0.391335881448281 V91Y-A96Y 0.39033918204701 A124Y-V159Y 0.390174150925501 L321Y-Y326Y 0.389820976996921 I85Y-F276Y 0.387740199498589 P512G-N112Y 0.386608717526294 V316Y-L380Y 0.385742423264415 I542G-F547G 0.385485965304967 P273Y-V319Y 0.385119839383092 F220Y-I385Y 0.383548029533444 V507G-L167Y 0.383497288459189 W170Y-I175Y 0.383287486040263 G454E-F228Y 0.380953318607739 I274Y-I399Y 0.379789289072507 I192Y-A395Y 0.379296617439253 A27Y-A190Y 0.379168320358916 I458E-F228Y 0.378652572556284 A269Y-A408Y 0.378504858708964 L266Y-L369Y 0.378224246982312 A27Y-A172Y 0.378076279434451 F219Y-I224Y 0.377042803051084 A74Y-A124Y 0.37618600409371 V93Y-E98Y 0.374592849552371 Y80Y-G118Y 0.373814352635891 L318Y-T323Y 0.373523255758282 V316Y-L377Y 0.372327364089714 V504G-V162Y 0.367007780322988 I458E-A408Y 0.366372358673808 L536G-V541G 0.365828344705359 T92Y-L97Y 0.363419800817959 R174Y-G183Y 0.363114194703052 L218Y-F223Y 0.361639455132895 V492G-L550G 0.36155631463471 V316Y-L321Y 0.360195235081598 V404Y-L409Y 0.359659443760613 L317Y-F322Y 0.359653476457066 Q88Y-P95Y 0.35809330890561 L469E-T474E 0.357885017098167 G470E-Y28Y 0.357762549013555 N182Y-V407Y 0.357543077441637 P35Y-S71Y 0.35717498825375 V504G-Y114Y 0.357080753520802 V378Y-I399Y 0.356869682117755 V475E-Y28Y 0.356835802745847 P79Y-Q282Y 0.356792172720874 L494G-V499G 0.356647283102786 I34Y-F70Y 0.355951981617886 V378Y-L402Y 0.354487383156658 Y472E-G191Y 0.354256697914974 D473E-L30Y 0.354143385535531 A551G-I34Y 0.351661246734523 V545G-L550G 0.350067652136463 L312Y-T379Y 0.348838566555606 M466E-V194Y 0.348620287961383 T82Y-A279Y 0.347807011192297 L381Y-Q386Y 0.347589833111082 T21Y-I175Y 0.347294488460937 V507G-L169Y 0.345261392100067 E195Y-G400Y 0.344586909021439 V499G-V546G 0.344484336817681 A124Y-A166Y 0.342969352544291 Q88Y-V93Y 0.340501496288324 P198Y-S393Y 0.340311253292879 A464E-A408Y 0.340044633249139 Y505G-G535G 0.33969755271142 S397Y-L402Y 0.338180198607633 A548G-A27Y 0.338030720023727 F33Y-F70Y 0.337386045880313 F228Y-A233Y 0.337003419896654 T455E-A232Y 0.335232641180067 T176Y-T184Y 0.33268003723953 I81Y-F276Y 0.330933605118147 Q199Y-H392Y 0.329263878926335 A548G-I34Y 0.32871754749879 P79Y-L122Y 0.328299760655784 M231Y-V319Y 0.328017438061129 Q113Y-G118Y 0.327944030598301 A269Y-A328Y 0.326645277030432 Y80Y-G119Y 0.325550689525706 V507G-A166Y 0.323675759318723 L491G-Y496G 0.323548894256279 L226Y-M231Y 0.322485587897423 F20Y-I175Y 0.322373509990344 I274Y-L402Y 0.321944768959506 F20Y-I180Y 0.321135803374325 I81Y-M87Y 0.318961292611942 M171Y-G183Y 0.316555738817918 A549G-G31Y 0.315590516636487 T82Y-T184Y 0.313448578557608 I274Y-F322Y 0.312861682192111 G181Y-G270Y 0.312503655768747 Q88Y-K99Y 0.312316168906884 Q383Y-H392Y 0.312127455656888 V18Y-L23Y 0.312099250768155 A551G-G164Y 0.31169132326449 A121Y-G164Y 0.311541866299942 L312Y-L380Y 0.311136142296319 G183Y-G406Y 0.310781099688199 Y472E-R29Y 0.310767691413125 G311Y-G376Y 0.310537060311707 I394Y-G400Y 0.31049614333254 N267Y-W370Y 0.308991065012389 L459E-A464E 0.308516425348347 V162Y-L167Y 0.307753345437326 F322Y-A372Y 0.305043668909793 Y28Y-V193Y 0.302912494919854 L266Y-V364Y 0.302683682412603 V319Y-A372Y 0.30105844416803 Y505G-V541G 0.300685656355244 E315Y-T323Y 0.300176947633215 R116Y-L169Y 0.300175559752983 M78Y-A279Y 0.299941965779827 L22Y-A27Y 0.299315897702281 L25Y-A190Y 0.297951918043326 Q510G-M171Y 0.297880889898835 A551G-A163Y 0.297159199471307 T21Y-A26Y 0.296980998621008 I274Y-A279Y 0.296579735597582 A277Y-G398Y 0.296351120741037 L503G-T540G 0.295705516750279 A227Y-G406Y 0.295443689941548 I224Y-A229Y 0.295130273376869 E511G-T115Y 0.295022807803555 I77Y-L169Y 0.294769221703245 T184Y-P273Y 0.294760043998141 T323Y-G371Y 0.294605628840964 L503G-L543G 0.293730768930079 V271Y-L402Y 0.292816613180218 P273Y-T379Y 0.291866582444534 A268Y-Q330Y 0.291852527118246 I72Y-V162Y 0.29145754203569 V234Y-W370Y 0.29142322402509 V316Y-L375Y 0.291317623940976 V234Y-L266Y 0.289610884332221 A74Y-A163Y 0.289024118175544 G376Y-L401Y 0.286879102504616 N267Y-G406Y 0.286318267537031 E98Y-R108Y 0.285488178765639 L168Y-E173Y 0.285463132025217 V467E-Y472E 0.285336749444085 L266Y-A372Y 0.2852628549956 G119Y-L167Y 0.285250401276305 P273Y-E315Y 0.284732451878099 L506G-T540G 0.284190830775547 L167Y-A172Y 0.283615426822639 M466E-R29Y 0.283056861408912 A27Y-G164Y 0.282947572617349 G498G-L550G 0.281864678015825 I272Y-A277Y 0.280999642994338 G454E-L226Y 0.280598877985936 K99Y-R108Y 0.280519708395696 V159Y-G164Y 0.280331335869166 R116Y-A121Y 0.280330225920199 V499G-V159Y 0.279821350375835 G76Y-A190Y 0.279808790159462 L543G-A548G 0.27972142598577 A395Y-L401Y 0.279711023396245 A227Y-L401Y 0.279230460243559 R116Y-L122Y 0.278945167103702 L469E-R29Y 0.278679271477852 L375Y-G400Y 0.278087282273814 G230Y-L401Y 0.276607904998712 Q88Y-Y326Y 0.276290267768009 L266Y-R366Y 0.27617654540917 Y472E-P198Y 0.276082326364143 G230Y-L402Y 0.275601944072326 G501G-L167Y 0.275539461117487 W370Y-L375Y 0.275507729419949 G205Y-L214Y 0.274562499337356 I188Y-I403Y 0.273308137731347 G127Y-A163Y 0.272932491713556 G470E-L25Y 0.272603342557094 A457E-A408Y 0.271397759723576 L22Y-G183Y 0.270199613755294 I274Y-I403Y 0.270182825015976 E315Y-Q383Y 0.269939244220696 Y505G-Q510G 0.269714847433916 L508G-G118Y 0.268223725745571 E511G-T176Y 0.267596159619203 L502G-V507G 0.265497233355527 A277Y-G311Y 0.264650969247226 R477E-A32Y 0.264042618871387 L380Y-G400Y 0.26329622257616 G376Y-S397Y 0.262597242883923 R538G-M171Y 0.262017262117109 W196Y-G400Y 0.260928602847661 M87Y-T115Y 0.260334614699642 L506G-G534G 0.259503080907008 V504G-V509G 0.258934748946993 V325Y-Q330Y 0.258803899745946 L469E-E195Y 0.25760644886192 F276Y-L281Y 0.256908031952268 I120Y-A166Y 0.255136173642793 R116Y-M171Y 0.25461452191008 G544G-W170Y 0.253756057514314 I394Y-I399Y 0.252669255739815 G501G-F547G 0.251821801878439 G76Y-A172Y 0.249322380314957 T323Y-A328Y 0.249215731929702 F223Y-G398Y 0.249139477456386 Y537G-I542G 0.248286667178948 P273Y-Q330Y 0.247171466082071 L221Y-P382Y 0.246895004998504 L122Y-G164Y 0.246499919954311 N267Y-Q330Y 0.246398118661144 G544G-Q161Y 0.246153200019996 A227Y-G376Y 0.245363229445504 L23Y-Y28Y 0.24507713606428 L508G-G119Y 0.244888720641882 E315Y-P382Y 0.244702007635285 Q199Y-Q386Y 0.244650219613419 Q456E-G230Y 0.244472882128249 G118Y-Q161Y 0.243706692913255 A121Y-A163Y 0.243139475760342 V378Y-S397Y 0.243020591482218 I403Y-A408Y 0.242647509365741 Q126Y-G164Y 0.242300355497123 L75Y-T82Y 0.242059982129345 T462E-L409Y 0.241644545638268 V467E-V193Y 0.241332309318802 Y472E-A32Y 0.240920451272349 F73Y-Q161Y 0.24090915719988 Q383Y-S397Y 0.240249924212359 T323Y-L367Y 0.239846287848754 L75Y-T160Y 0.239322210712296 P35Y-I72Y 0.237806564584327 A268Y-Y326Y 0.237217784977938 G230Y-Q235Y 0.235460206901587 A32Y-G164Y 0.235381415047708 L469E-P198Y 0.23404666716027 F70Y-G127Y 0.233678402274074 V215Y-I385Y 0.233317592314985 A457E-G406Y 0.233085428153764 T462E-V193Y 0.233005302650406 S185Y-G400Y 0.232995553200523 G311Y-G398Y 0.232846312406758 T82Y-L281Y 0.232400368674501 R116Y-Q126Y 0.232316700578784 G470E-Q199Y 0.231960473951962 G230Y-N267Y 0.231602870779767 I274Y-V405Y 0.230759445118064 T462E-L401Y 0.230548040206172 R29Y-M171Y 0.230404194595776 I120Y-F125Y 0.229874741351516 S71Y-Q126Y 0.229466235841793 V24Y-R174Y 0.229401418690675 P35Y-L75Y 0.229297285584928 Q282Y-E315Y 0.228393562734044 N267Y-L369Y 0.22831075949289 L502G-G164Y 0.228252257367405 L25Y-T184Y 0.227714509364099 G181Y-D410Y 0.226903946488238 H392Y-G398Y 0.225961872205307 L266Y-T327Y 0.22552214468371 Y505G-G119Y 0.224870315773764 L469E-A190Y 0.224589399135748 F219Y-Q383Y 0.223156427804075 F461E-A190Y 0.223069705326468 T82Y-Q113Y 0.222480516744298 T379Y-Q386Y 0.222301040581197 Y505G-P512G 0.22226414339699 G544G-I165Y 0.222124125488935 I188Y-V405Y 0.220911564122395 V499G-V504G 0.220606184967492 M466E-I192Y 0.220021314198096 Y326Y-S365Y 0.219812883017696 L503G-T160Y 0.219108335295554 G270Y-D410Y 0.219077952208009 S185Y-V407Y 0.21896603888242 G501G-V158Y 0.218424412456462 V271Y-V407Y 0.217999286798225 L75Y-G119Y 0.217849795695322 A551G-P35Y 0.217488397468324 M466E-L471E 0.217391954754202 Q456E-Q235Y 0.217043758584387 E315Y-L321Y 0.216613843096162 A551G-F70Y 0.216203724997893 Y28Y-A172Y 0.216007522847644 I385Y-G398Y 0.215511652974083 M78Y-W170Y 0.215400492635647 I274Y-G376Y 0.214322890162959 I81Y-G123Y 0.214315261730636 I224Y-V405Y 0.214075308660545 F70Y-G164Y 0.213667353773753 A27Y-S71Y 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