[Wed Aug 24 17:52:25 2016] >Input file: TM5C76ATD.pdb >TM segments: A17-39_A74-98_A148-170_A175-208_A238-270_A294-318_D83-112_D139-165_D175-197_D253-274_D296-326 MAIDEN score = -121.5887 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- A98D-I250A 2.31489541152958 C269D-Y83A 2.19130548481292 Y83D-C269A 1.77750897562984 C269D-I80A 1.25335055487882 I188A-L193A 1.24045698588492 E264A-C269A 1.16887527191377 L153A-I310A 1.10581182239312 L153D-I310D 1.10309098128702 H95D-I250A 1.08299087729677 F268D-Y83A 1.03632570289178 F23A-Y93A 1.00364424328758 Y83D-F268A 1.00341329358004 Y110D-F238A 1.00120478540075 V272D-I80A 0.991342541190929 R203A-A248A 0.979468398809482 F29A-V159A 0.966139819327851 E264D-A87A 0.962158778900788 A87D-E264A 0.948181208059418 E156A-A307A 0.936144333687792 E156D-A307D 0.915271399493788 A186A-K191A 0.912149112474573 S28A-V159A 0.868010710828669 E264D-C269D 0.842798588386065 I35A-L298A 0.810885176412401 I188D-L193D 0.80932380996584 I197D-V255D 0.802593230302333 L21A-L151A 0.800139478444969 H106D-K239A 0.756866046112903 N90D-I261A 0.747241817254547 H106D-F245A 0.747006540974866 V25A-F158A 0.735814920885484 T32A-V159A 0.708277524954321 K105D-L143D 0.705913956516292 E31A-V159A 0.70470919822352 R204A-N252A 0.679647847778261 A34A-Y83A 0.670654518999543 S36A-I295A 0.663868734246194 R104D-V140D 0.663390962674657 I108D-V140D 0.662695981162246 K102D-Y141D 0.653982353126327 L153A-Y314A 0.651437975182874 F187A-I192A 0.646626754231999 I157A-I310A 0.640430895743494 V160A-L303A 0.636041593063436 M146D-H321D 0.631908011313078 I157D-I310D 0.627830819558653 F273D-G76A 0.624645658405213 I35A-A299A 0.61443230276349 V160D-L303D 0.60966189709139 F94D-A257A 0.604567986237526 S28A-F158A 0.603207378339746 L20A-L151A 0.597358712495829 H95D-T251A 0.595564886984047 L193A-P259A 0.586026124036774 F187D-I192D 0.581798833877589 M208A-F245A 0.579972274524356 I266D-Y83A 0.578844891555577 M167A-F296A 0.57783475503228 A34A-F82A 0.576292343612527 L143D-H321D 0.576229062972202 A87D-V258A 0.564862082806222 L143D-Y320D 0.564026241663001 N90A-H95A 0.561832177716878 F94D-E253A 0.55047721171212 M167A-A299A 0.542941849589243 C269D-L79A 0.535495278945529 I197A-N252A 0.533946549017962 I261D-A91A 0.523420956798198 A98D-N249A 0.523034043127444 S149A-L317A 0.522989380177696 F150A-L317A 0.51370989604445 F150A-D316A 0.504855697073669 F150D-D316D 0.504242543237287 R204A-I250A 0.504220043532603 A87D-I261A 0.50407386335468 R260D-I266D 0.501184023008973 F150D-L317D 0.500552820019825 V27A-Y93A 0.49812229917767 A257D-F94A 0.496996948464467 E253D-P259D 0.49368774734205 I30A-R86A 0.493209557281611 F29A-L162A 0.491343325626973 L317D-S322D 0.490532319716116 K111D-K239A 0.487359455577408 G103D-K247A 0.486403970516464 V27A-N90A 0.485702213949352 E253A-P259A 0.485141260482553 E31A-P305A 0.484377345338622 L20A-Y93A 0.480972811552689 E264D-P305A 0.479016403639777 Y164A-T175A 0.477131021923597 L152A-R309A 0.473103002312453 K105D-K111D 0.462198135825683 A98D-T251A 0.460789514237177 L152A-S313A 0.459993225460176 E156D-I310D 0.459155757496963 L189A-P259A 0.457314037006254 L189D-P259D 0.451217147941406 K102D-K247A 0.44891153274747 V84D-C269A 0.447539089886806 R86D-I261A 0.444327040287702 L20A-L97A 0.442470842029682 E156A-T312A 0.440522743496504 A91D-V255A 0.434945952420282 R260A-I266A 0.433686832995338 F273D-I80A 0.433267029081325 L152D-R309D 0.430410340037323 V27A-R86A 0.429882371022246 L193D-P259D 0.429664915556236 E156D-T312D 0.427429773382212 R204A-F245A 0.422667984192875 S101D-Y141D 0.421527691965785 T32A-L162A 0.421047241389081 K102D-F245A 0.419623121601846 R104D-S144D 0.419309776967848 S149A-Y314A 0.41850761672746 V27A-S155A 0.411774327762066 A257D-A91A 0.411346826079565 K105D-Q241A 0.41098557081551 E156A-I310A 0.410264419277821 V297A-R302A 0.410244606528535 V272D-V77A 0.409894154482219 I266D-V84A 0.404118720494614 S149D-L317D 0.403509141607486 C269D-E31A 0.398852785323192 V160A-A307A 0.396732972020448 H106D-Q241A 0.39548524876412 V160D-A307D 0.394432484155799 T145D-E253A 0.394259035364415 V25A-V159A 0.384411527504479 R104D-L143D 0.383650474378618 L21A-M154A 0.381781911168721 A98D-A257A 0.379190854275884 K102D-E253A 0.376703206020963 S24A-Y93A 0.375051383954037 V159A-R302A 0.374377298485937 Y164A-A299A 0.374141249137436 Y83D-I266A 0.372423192906499 L97D-S148D 0.369614462947774 A107D-A240A 0.367846466497697 V160A-R302A 0.363586108195232 V272D-L79A 0.362387843094529 I180A-N185A 0.357801684686217 F177A-C269A 0.357417290516924 K199A-E205A 0.356975952054526 H315D-S322D 0.355936800887734 L163A-R302A 0.355587802953782 L163D-A299D 0.354659368444335 V297D-R302D 0.350841530256939 S148D-H321D 0.349598793388829 L153A-S313A 0.349013056160534 F268D-I80A 0.348133712600005 C269D-G78A 0.346928802749769 L153A-R309A 0.343463400448682 K105D-K247A 0.342975796555312 R99D-S246A 0.342317119024683 S254D-F94A 0.341517798556331 A98A-S148A 0.340715354877152 L153D-S313D 0.340402275507393 F245A-E253A 0.337404974357085 K102D-N252A 0.337389114533405 V159D-L303D 0.336822080986156 V81A-R86A 0.336190936943781 F177D-C269D 0.336178168391014 A186A-A307A 0.335706027786742 A107D-K239A 0.335701067422734 L163A-A299A 0.33535186159548 N90D-H95D 0.332628698104459 S254D-A91A 0.331983017145208 V159A-L303A 0.331361027793895 K191A-I196A 0.330416045420797 Y92D-S254A 0.326687533867365 E206A-Q241A 0.325902416165304 A98D-S148D 0.322444639218951 A91D-G265A 0.321782631984218 L20A-L96A 0.318894641331438 M146D-Y320D 0.318006915469197 L143D-L324D 0.317833510011155 T32A-E156A 0.317578029228985 Y110D-S242A 0.317511687024028 F150A-S313A 0.315265698872365 N185A-R260A 0.314847304938556 Y301D-V297A 0.31382347981079 I157A-R309A 0.313678233789407 H106D-V140D 0.313389497925979 K198A-R204A 0.313188347945168 A186D-A307D 0.313012906694836 R104D-A109D 0.312680867587076 S101D-I250A 0.311377783860838 V190A-P195A 0.310771814570169 V190D-Y262D 0.310415473353504 N142D-H321D 0.309028469079574 V159D-R302D 0.308660238484689 Y83D-R260A 0.307683158916771 E253D-L97A 0.307177787818573 A34A-G78A 0.306684235783135 L189D-S194D 0.306667275747511 H95A-S148A 0.306253087020126 C269D-V84A 0.30332638443738 P259D-Y92A 0.303063438329092 I157D-A307D 0.301338037788053 A200A-T251A 0.300250083193426 Y83D-V258A 0.30024218175863 V38A-I295A 0.298682148776793 L163D-L303D 0.297458080296514 L168A-L176A 0.29713066220313 S36A-A165A 0.297006625208088 L163A-L303A 0.296851721688872 V190D-P195D 0.296373685496684 L152D-S313D 0.292459302844555 L163D-R302D 0.291140110423203 R99D-Y141D 0.290066123635455 G267A-L300A 0.289041401300529 K102D-S149D 0.288516947048211 R104D-E243A 0.288157644503986 S155D-P305D 0.286908313271417 V255D-H95A 0.286653424320825 A34A-L79A 0.286590547754489 I80A-R86A 0.286469027985277 A207A-A244A 0.285125501270981 H106D-M208A 0.284730903259383 M146D-L317D 0.284125386701778 V160D-R302D 0.283814284593134 G201A-N252A 0.283790971132524 H95D-V255A 0.283337067019749 A186D-K191D 0.283005766762935 V190A-Y262A 0.280815557054125 V112D-V140D 0.280635908798787 M146D-R309D 0.280344457116195 R86A-A91A 0.278879303424932 N185A-C269A 0.277645198729018 N185D-R260D 0.276241291323108 T32A-L163A 0.275587673100409 H95D-S148D 0.273651261535868 F94D-S254A 0.272591835616858 I266D-A87A 0.270307064156047 M39A-I295A 0.269081927067393 V27A-L89A 0.26730870300683 V159D-S306D 0.266712223646053 E243A-N252A 0.26612319107568 I197A-V255A 0.265876331840378 V25A-M154A 0.265860957322014 K105D-Y110D 0.265106603982811 I157A-A307A 0.262651750903481 S24A-L152A 0.260472841043058 H95D-K105D 0.259994292801441 E156A-S306A 0.259358783941639 P305D-S313D 0.259097976420341 R86D-Y262A 0.258207554796041 S194A-G201A 0.257932691023668 S24A-M154A 0.257859861707074 S155D-A307D 0.257250860128745 L21A-S155A 0.257245204022645 K102D-A207A 0.255625869164162 A165A-S179A 0.254873301174019 G267D-L300D 0.254257060299905 N142D-Y320D 0.253308335922972 V25A-E156A 0.253028646371335 F100D-E243A 0.251770620301037 A91D-I261A 0.25158811148764 N252A-P259A 0.251188682804516 E156A-H315A 0.250781673064573 R99D-K105D 0.250396046283589 Y93D-S148D 0.250035627588225 Y262D-R86A 0.249297212166319 Y93A-S148A 0.249257381639868 L153D-Y314D 0.247889462632387 F150D-S313D 0.246956085191439 P305A-Y314A 0.246274813647816 Y164D-A299D 0.245548511103658 L17A-S148A 0.245067161554736 A109D-S246A 0.243776845882138 S254D-N90A 0.239950469108712 I261D-N90A 0.238434121770094 F181A-A186A 0.237673580034945 G103D-Q241A 0.237036193669431 Y164D-T175D 0.236699800782144 A34A-A87A 0.236184924255697 N185A-M304A 0.236123703362162 K102D-R204A 0.235339592095931 A87D-F94D 0.2337838643933 M154D-D316D 0.232853585169685 A256D-Y262D 0.231952065812448 I197A-R203A 0.23096993704128 I261D-R86A 0.230204517978429 E156A-R302A 0.229935342113177 I188D-V258D 0.228812747646221 S24A-S313A 0.228359449808246 H106D-K111D 0.227285417171444 G267D-Y301A 0.227223375098026 V84D-E264A 0.227025157271517 L153D-H315D 0.226793376492137 S242A-N249A 0.223724047298841 F33A-A299A 0.222356009552648 F23A-E31A 0.222100448729483 Y92D-P259A 0.222034021659886 N185D-G265D 0.220760929087989 M167A-R302A 0.219458046779126 K199A-A256A 0.219417730428866 R104D-M146D 0.219310525419208 I157D-R309D 0.218675237198216 A207A-A248A 0.218076649445993 N142D-L324D 0.218033349671011 N185D-C269D 0.217892054807405 N308A-H315A 0.217385183697965 S148D-S246A 0.216662518102331 E156D-R302D 0.216301201964299 S155A-A307A 0.216072741136665 H106D-T145D 0.215732270151409 V159A-S306A 0.215576776215904 Y262D-F82A 0.215532941081699 Y262D-V81A 0.214522518687488 T145D-H315D 0.212609886333712 Y164A-P305A 0.212030897163494 N142D-S149D 0.210994966456448 V27A-F85A 0.210482066581406 R104D-Y141D 0.210266955906694 V25A-L151A 0.209583222421818 R99D-A107D 0.209199965308983 Y164A-S306A 0.207739644191367 L97D-K102D 0.207194998050112 K102D-Y110D 0.206222351623491 E264D-Y83A 0.205996915613157 I35A-I295A 0.205871616867539 I188A-C269A 0.205590345547366 H95D-S246A 0.204731202029974 P305D-Y314D 0.203251205426105 V22A-L151A 0.202862690244802 L143D-N325D 0.202666736398836 I192D-V258D 0.201341521269955 L37A-M167A 0.201237958432071 I196A-G201A 0.20032520821475 V183A-M304A 0.200025403596662 V258D-L88A 0.199395889895976 S148D-S322D 0.199225912377007 V160A-M182A 0.199177983826497 I157D-S306D 0.198270392452856 F273D-G78A 0.198146698105022 M154D-N308D 0.198071724826772 L153A-H315A 0.197942657872278 R260A-C269A 0.19786337722534 V159A-Y164A 0.197567902253565 L97D-S149D 0.196377561873458 N185D-L263D 0.196121871921781 I261A-G267A 0.195742049393009 G103D-S148D 0.195121389827036 E31A-L163A 0.19498460706014 R104D-I147D 0.194937017665531 P195D-S254D 0.193713870747107 Y164D-P305D 0.193666323276798 P305A-S313A 0.193420289748365 F18A-V25A 0.193362677572126 G265D-R86A 0.193213412333147 M167A-T175A 0.192620465533105 K198A-A248A 0.192035370293068 F75A-Y83A 0.191968333266883 S24A-L151A 0.191858395696417 I261D-G267D 0.189844530053865 R86D-A91D 0.188491661227634 M146D-S155D 0.18822458402586 S28A-M304A 0.187425405732816 S36A-Y83A 0.187408249492727 I196D-V255D 0.187118698033444 N185A-G265A 0.187085167178793 Y92D-Y262A 0.186753042703228 S24A-H95A 0.186712242704688 V272D-F82A 0.186513583130352 E205A-T251A 0.186494250127837 L318D-N325D 0.185948059124888 M182D-S306D 0.18558890965354 A107D-Y141D 0.185461278508779 S148D-Y320D 0.18533401039823 F29A-Y83A 0.184634831179647 S148D-N252A 0.184488326316578 L152D-P305D 0.184289370588127 S28A-Y164A 0.18381888469609 F268D-E31A 0.182569603782141 R104D-K111D 0.181824074452181 L37A-L79A 0.181821186936699 M182A-F187A 0.181685453745923 F33A-F82A 0.18167117999382 G267D-A34A 0.181590728495554 V270D-G76A 0.181538422610148 S149D-S254A 0.181230148285165 I261D-A87A 0.181050430615444 R86D-R302D 0.180992944624836 I30A-A87A 0.180942690606414 K105D-A240A 0.180890734652346 E156D-L162D 0.179467095542679 L298A-S306A 0.179304528690431 L298D-S306D 0.178949528896133 E139D-Y319D 0.178877065718762 Y141D-N325D 0.178415977585948 Y301D-G267A 0.178128115615632 Y141D-S323D 0.178037533215712 V38A-Y301A 0.177977892880602 E31A-Y301A 0.17741209560318 S36A-L168A 0.177382814117322 I261D-C269D 0.177177482877114 Y83D-E264A 0.177160266912222 S155A-Y314A 0.17677976852723 S144D-S242A 0.176702133418676 S254D-L89A 0.176687782162952 F94D-K102D 0.176447717373306 S28A-L162A 0.176319908368441 K102D-Q241A 0.175740063869712 R260D-P305A 0.175267548326205 V297D-Y301A 0.175078410042413 Y110D-E139D 0.174715974230172 V38A-F296A 0.174467018676796 M208A-N252A 0.174073866848824 E31A-V38A 0.174004957291354 L193A-G201A 0.173711808971366 T145D-T251A 0.17350459577425 S28A-P305A 0.173039858863288 S254D-Y262D 0.172858952371113 L151D-S306D 0.172616047234955 S246A-N252A 0.172314369173455 E31A-Y93A 0.171126389568556 F273D-V81A 0.171075483447451 K105D-F238A 0.170621745468306 F100D-K105D 0.170431369412039 L97D-K247A 0.17009144102343 Y93A-S149A 0.170052462042992 P259D-R86A 0.170027674095221 S28A-Y93A 0.169748754351394 E264D-V84A 0.168565918969422 R86D-G265A 0.16836946549616 H106D-L143D 0.168151727435687 F19A-Y92A 0.168090117446088 R309D-D316D 0.166630069958274 S148A-R309A 0.166354191695966 S254A-Y262A 0.166192951228426 V258D-R86A 0.166013482359194 P259D-L88A 0.165988229254671 R204A-E253A 0.165824914039256 S194A-Y262A 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