[Wed Aug 24 18:01:27 2016] >Input file: TM5CH4ATD.pdb >TM segments: Y19-39_Y51-87_Y114-135_Y151-175_Y183-208_Y211-231_Y271-292_Y296-322_E34-59_G3-25_G52-72 MAIDEN score = -116.6507 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- P35Y-S71Y 10 I207Y-V212Y 2.19667255530558 I285Y-A301Y 1.64119648668223 P37Y-G71G 1.25093342818064 I120Y-I165Y 1.24763198079383 I207Y-L214Y 1.22231253286484 L61Y-I192Y 1.22208988995029 I77Y-I165Y 1.10957471253813 I34Y-I72Y 1.1049970007075 I58Y-Q282Y 1.09697351091648 P79Y-L122Y 1.05518838331438 F128Y-W154Y 1.05433271458565 Y80Y-T115Y 1.04618307404373 F134Y-P290Y 1.04029533599944 Y12G-F63G 1.03460540502101 W170Y-V23G 1.02907170604286 V39Y-F70Y 0.982960538960725 V193Y-F41E 0.976658468353844 I34Y-L68G 0.959945301885016 A277Y-E315Y 0.952725346732533 P284Y-A301Y 0.940686805025712 F304Y-G311Y 0.912589909188455 P37Y-A67G 0.894802433421994 A83Y-R116Y 0.892438022577165 Q292Y-Q298Y 0.882430395790692 Q292Y-L297Y 0.878786115353204 F73Y-I165Y 0.874180772786305 V194Y-D49E 0.871511534878318 A32Y-R69Y 0.859509662066905 Y80Y-I120Y 0.832207698186272 V15G-F63G 0.81313911287022 L130Y-L135Y 0.806334495306539 I120Y-V162Y 0.803700558192562 I274Y-V319Y 0.794691992492314 F128Y-V157Y 0.776381901731799 I77Y-L169Y 0.775881146143043 L30Y-F52E 0.741135575276595 S63Y-L75Y 0.739686008115132 A278Y-E315Y 0.730858256304714 I77Y-T184Y 0.723225456641249 L19G-L59G 0.717217380231645 L130Y-P290Y 0.69918606347651 L30Y-Y48E 0.691115580081105 S63Y-I188Y 0.688458944966412 N302Y-R308Y 0.682432923992316 A124Y-V162Y 0.682004612918419 A277Y-F322Y 0.680342700318863 I34Y-S71Y 0.678463439141208 S63Y-I77Y 0.673313643100284 A289Y-L297Y 0.672521444403752 A83Y-L122Y 0.671796339100285 I85Y-F276Y 0.671327785461006 Y80Y-L122Y 0.666736139116208 F62Y-I192Y 0.666610213956549 A16G-F63G 0.665504487845777 G64Y-I188Y 0.662947328484266 L206Y-V212Y 0.662703404656128 Y80Y-L169Y 0.661833549942255 I285Y-F304Y 0.660865978797318 F134Y-F291Y 0.65996003984081 V159Y-L13G 0.658100439286547 P79Y-Q126Y 0.651251952097448 F304Y-I314Y 0.64962713419848 F128Y-V158Y 0.63900314471612 I81Y-F276Y 0.637426744498955 A32Y-F73Y 0.635169128493148 I77Y-L168Y 0.631980627266779 V194Y-L45E 0.630939667969654 M78Y-A279Y 0.630487128463575 P37Y-L68G 0.628069200210993 V55Y-A289Y 0.623080547659009 P37Y-L72G 0.622764148252465 I274Y-F322Y 0.62243223356518 W196Y-M42E 0.620395345758847 V212Y-F217Y 0.617195689656535 F62Y-G76Y 0.617177301356586 A133Y-F291Y 0.613210160922513 F67Y-L75Y 0.607692590037169 P273Y-F322Y 0.607511920206433 A32Y-I72Y 0.606983851061909 F134Y-A289Y 0.606731244790863 G76Y-I165Y 0.602419830923703 P35Y-L68G 0.599859602787207 I34Y-A64G 0.599728476937552 T36Y-V157Y 0.59703728102247 L167Y-V23G 0.592451870400268 P35Y-G71G 0.592269116595338 F304Y-S310Y 0.592047478220796 A26Y-L45E 0.583352061800992 V159Y-Y12G 0.56835994335578 R29Y-I34Y 0.567569456566851 F134Y-Q292Y 0.56721454906837 A131Y-F153Y 0.565006364380123 F56Y-A289Y 0.564782920015555 F62Y-G191Y 0.562177047034456 I81Y-R116Y 0.561724149502165 T82Y-F276Y 0.560388520543057 P37Y-I70G 0.552036806777708 N66Y-I72Y 0.547916861531183 L30Y-D49E 0.547351501628425 P35Y-F70Y 0.545525464952157 G38Y-F153Y 0.540689044659638 I81Y-A279Y 0.540454877857176 I34Y-A67G 0.536978415101132 Y80Y-G118Y 0.530009372788205 F304Y-L312Y 0.528944981645423 D49E-F54E 0.52792751795888 V194Y-M42E 0.519985598395765 S63Y-G191Y 0.512717615092342 A190Y-F41E 0.510336928762455 V15G-L59G 0.509148682486193 R116Y-L169Y 0.502800279269819 F228Y-I34E 0.501364176269465 Y12G-L66G 0.500951148321995 A172Y-I187Y 0.496018500559358 Y28Y-L168Y 0.494783271386755 I117Y-L24G 0.494728602823502 T160Y-A67G 0.494597112452507 S185Y-I272Y 0.482298850287374 F291Y-Q298Y 0.4783687479078 F62Y-M78Y 0.476655334150268 F62Y-I188Y 0.475790490020478 F33Y-D49E 0.473691179845659 P273Y-E315Y 0.473473414880298 F33Y-R69Y 0.4722775656842 F33Y-S71Y 0.471426562021839 A166Y-L19G 0.464876312876815 I58Y-P306Y 0.461479513738235 R69Y-A74Y 0.459972747170419 I58Y-N305Y 0.459529907199482 I59Y-F286Y 0.458977096437827 L25Y-I187Y 0.45886258005067 Y12G-A67G 0.452661971853265 V24Y-I175Y 0.450116513708902 I285Y-N302Y 0.448885503984958 I285Y-N305Y 0.448087770984036 F303Y-R308Y 0.44780680134625 T82Y-G119Y 0.447072555403144 T203Y-V212Y 0.443917462635584 A131Y-V157Y 0.44383337380679 G119Y-I165Y 0.443784370984306 F62Y-L75Y 0.442703780659562 V193Y-T38E 0.44217037204535 A124Y-V159Y 0.440954945279928 F20Y-I175Y 0.437602939799636 F33Y-R53E 0.436373542986719 A131Y-W154Y 0.433068843398452 P284Y-E315Y 0.432523051980181 Y80Y-Q282Y 0.431512193418319 P79Y-I120Y 0.429488727791907 R53E-L58E 0.422546593944872 F228Y-L35E 0.422361765278797 R116Y-L24G 0.420830816395361 R29Y-L45E 0.420341276121767 L281Y-F304Y 0.420041509577035 P35Y-A67G 0.419030152424037 T21Y-E173Y 0.417514476123469 M171Y-I187Y 0.415101092224444 S185Y-V271Y 0.414350041028344 I34Y-F70Y 0.412515426135201 I59Y-F67Y 0.408385572861956 F73Y-L168Y 0.402151207507029 S63Y-M78Y 0.399989102679458 E173Y-G183Y 0.397311114878469 F291Y-L297Y 0.396928852186834 F276Y-F322Y 0.396198457262327 V55Y-A301Y 0.395458513996722 V162Y-A16G 0.394779786991396 A26Y-D49E 0.394298867083761 I120Y-A166Y 0.393040070138424 A121Y-V162Y 0.390022253136203 G31Y-S71Y 0.389473313178342 T36Y-E68Y 0.38517697887855 M78Y-I165Y 0.383645072753707 G64Y-V194Y 0.383507327784313 I9G-I70G 0.382946104340357 I81Y-G119Y 0.382169573327955 V193Y-L45E 0.380931982917426 I59Y-M78Y 0.377855746503793 A74Y-A163Y 0.377809806828855 F303Y-S310Y 0.377651408963389 T160Y-A16G 0.375725350530268 V24Y-R174Y 0.373618221624535 W196Y-V43E 0.373412949360132 P35Y-I72Y 0.372730208980504 E195Y-T50E 0.371450607769928 F33Y-I56E 0.371415932774143 A51Y-A289Y 0.370918303375992 L22G-L52G 0.369251648787489 L19G-T56G 0.368784500967875 A190Y-L45E 0.36843096013275 R54G-L59G 0.36608372853277 T36Y-A67G 0.362591545592207 L167Y-L19G 0.362298848557624 L169Y-V23G 0.361433424489484 G164Y-Y21G 0.358988171558956 Y28Y-G46E 0.358597113249639 V24Y-Y53G 0.356203274615918 L25Y-A190Y 0.355499367384613 V15G-V62G 0.35276769460499 L30Y-R53E 0.352703302856196 F33Y-F52E 0.351700840877134 I192Y-M42E 0.350404277982447 I72Y-T160Y 0.347548630592619 M78Y-I275Y 0.344143919918184 A288Y-I300Y 0.342319227465545 I274Y-L318Y 0.340286626043648 P35Y-N66Y 0.339013225180345 V24Y-R29Y 0.338956613300782 V20G-Y53G 0.337867914018394 V24Y-M42E 0.336433635386711 V55Y-I285Y 0.331282478567433 A121Y-G164Y 0.329578343522873 F129Y-F134Y 0.327727826784075 A51Y-A301Y 0.326681799098335 A26Y-G191Y 0.323030408527963 V159Y-G17G 0.320515925139562 G31Y-I72Y 0.31516760456693 N60Y-P306Y 0.315091661294549 A278Y-G311Y 0.315012758245628 L167Y-G60G 0.315011622325865 N66Y-F73Y 0.31416196297794 Y80Y-I165Y 0.31367763445529 F56Y-I285Y 0.313118347961967 R208Y-L214Y 0.312441883199027 I77Y-I188Y 0.312275151526893 I59Y-Q282Y 0.31227355267403 L19G-G60G 0.31167848745931 V8G-L66G 0.310555983503352 L169Y-T184Y 0.31039149622459 G76Y-G183Y 0.309826214628973 G76Y-A190Y 0.309170303559015 P284Y-F304Y 0.308521728892673 F125Y-V158Y 0.308114482149344 F73Y-G164Y 0.306792496570207 P37Y-Y5G 0.303864785696235 A74Y-Q161Y 0.30149990312977 L197Y-M42E 0.300861414126913 Q52Y-A289Y 0.298096914935326 A166Y-L24G 0.297621732838886 Y28Y-P35Y 0.296844697744852 G38Y-S71Y 0.296773698795701 V159Y-Y21G 0.29610762255149 G119Y-A163Y 0.295710914321266 Y21G-V57G 0.295345268889285 A74Y-A124Y 0.294873319410137 G31Y-G191Y 0.29483982507117 L167Y-V20G 0.294544687722646 G54Y-G299Y 0.294342397703922 L25Y-L45E 0.293882086676571 G57Y-G311Y 0.293497411983099 Q161Y-A166Y 0.290852827574464 A163Y-L19G 0.290496591377197 A163Y-G17G 0.289612431733823 M171Y-G183Y 0.289424130458184 A74Y-A131Y 0.286381928202957 G64Y-L186Y 0.285948897960612 Q282Y-L287Y 0.285160306569146 L25Y-G64Y 0.285036167250423 L75Y-G191Y 0.284871297473976 T82Y-P273Y 0.28296119883695 G76Y-A279Y 0.282210102423599 G54Y-L61Y 0.282203660556703 V8G-I70G 0.282032591425902 G14G-L66G 0.281672829602617 G123Y-L167Y 0.281572014190446 G53Y-G299Y 0.280951475633534 M171Y-R54G 0.279689283712667 L75Y-G119Y 0.278618419329999 N66Y-G164Y 0.278537231403839 I72Y-I165Y 0.277905144946967 V193Y-M42E 0.27734895167595 P79Y-Y114Y 0.275668124705206 M78Y-Q161Y 0.274670081342758 N66Y-G76Y 0.274353332772323 G57Y-A289Y 0.273888713385009 L197Y-R202Y 0.273837057292052 F128Y-F155Y 0.272554989916708 R116Y-M171Y 0.272442240066863 G57Y-N66Y 0.270846934247501 L22G-T56G 0.270602834510008 V24Y-D49E 0.270543708721454 V193Y-V43E 0.270351230535729 R29Y-F52E 0.269684408671576 L169Y-R174Y 0.269396059283906 G76Y-L122Y 0.267791855589937 L135Y-P290Y 0.2652431838802 A124Y-G17G 0.264100768700554 P79Y-I165Y 0.262022604835789 G65Y-W196Y 0.260424925812645 L61Y-G191Y 0.259180814089064 E195Y-T38E 0.259125765999845 I72Y-A163Y 0.258163793692845 R116Y-I165Y 0.258090858207844 G119Y-W170Y 0.2569304282447 L197Y-V43E 0.256418045087199 F291Y-G299Y 0.255331565603513 L25Y-G46E 0.255003844121342 P284Y-Q292Y 0.254118698839121 G65Y-G46E 0.252729835796028 G64Y-W196Y 0.251270479957559 T36Y-F70Y 0.250373499178943 I120Y-A163Y 0.25031999045316 T82Y-I280Y 0.249918706485783 A84Y-R116Y 0.24964931159934 I81Y-I275Y 0.249433737126852 L201Y-G46E 0.248540089899662 A190Y-A40E 0.246246542224949 Y28Y-V51E 0.245752119561095 Q126Y-G164Y 0.244585029515636 R116Y-Q126Y 0.244502136586818 G123Y-I165Y 0.243945523930549 Q199Y-R208Y 0.242429012345599 T203Y-L221Y 0.242155100404019 T21Y-L45E 0.241955524872254 T115Y-T184Y 0.241684169013825 L30Y-P35Y 0.241378197122456 G54Y-Q292Y 0.240585562731866 P79Y-L168Y 0.240110360905129 Q52Y-F134Y 0.239802925115592 F70Y-G164Y 0.239282833445934 F153Y-Q161Y 0.238715340307162 M78Y-R116Y 0.235436385662636 W196Y-T50E 0.235412206366817 L186Y-T38E 0.235040588931058 A32Y-F70Y 0.234801947951798 L24G-T56G 0.234112325727343 F129Y-A289Y 0.233168592887214 L25Y-T184Y 0.232918339720013 L75Y-Q161Y 0.23210428370373 F304Y-E315Y 0.231913223028782 R116Y-A172Y 0.231480380077191 Q52Y-F303Y 0.231139423185614 A26Y-F33Y 0.230497616491867 G14G-G60G 0.230129916035254 P79Y-Q282Y 0.230055193234765 A51Y-R308Y 0.229980262984546 A288Y-L297Y 0.229836514696767 G205Y-N213Y 0.228983049122162 G53Y-F134Y 0.228064790336761 P79Y-L287Y 0.226838298273057 T115Y-L169Y 0.226321880670974 L75Y-T82Y 0.226200725857965 A278Y-L318Y 0.225570437994529 F70Y-A131Y 0.225420565625411 P79Y-L167Y 0.22494012368025 P198Y-L39E 0.224826965167453 T160Y-L19G 0.224536745235021 F33Y-A65G 0.224503218954565 V194Y-I200Y 0.223986401131119 G31Y-Q161Y 0.22371644294629 Q126Y-A131Y 0.223487003109585 F67Y-G191Y 0.223349497738742 G119Y-L167Y 0.222944246459146 P306Y-F313Y 0.2225284714367 A131Y-F291Y 0.222083373969324 G118Y-V162Y 0.221821862068509 G65Y-I192Y 0.220766954764684 N60Y-N66Y 0.220344620905951 G64Y-G46E 0.219434869324577 P37Y-V156Y 0.21849501899796 I81Y-M87Y 0.217895740477132 V162Y-V20G 0.217815243890239 V23G-T56G 0.216729904545629 Y80Y-V23G 0.216193272964924 L281Y-I314Y 0.215393047633913 I120Y-G17G 0.214786120204638 G38Y-V69G 0.214770753774106 A133Y-F286Y 0.214528907003086 T160Y-L68G 0.214205877632101 R29Y-M171Y 0.213896554113092 L75Y-Q126Y 0.213870831251475 T203Y-F217Y 0.213671597956278 G54Y-E68Y 0.213430394677752 A172Y-T184Y 0.212934897239693 Y28Y-V193Y 0.211755552379508 G119Y-T184Y 0.210684083669161 V162Y-L19G 0.210062126898666 L30Y-V51E 0.210059693188565 T36Y-W154Y 0.209088629656251 R29Y-M42E 0.208406822112826 P37Y-L13G 0.208159110205168 F73Y-V157Y 0.208060789802344 G123Y-V20G 0.208006004815875 I72Y-G164Y 0.207930975704171 G299Y-S310Y 0.207918606148384 A190Y-M42E 0.20773731184163 G76Y-L168Y 0.207620104697488 F152Y-Y12G 0.206196401155573 T184Y-I274Y 0.206150558324324 L19G-V57G 0.205810244676981 P306Y-I314Y 0.205439956128944 L130Y-V157Y 0.205002781840782 E195Y-L45E 0.204712449582297 T82Y-I285Y 0.204644764887606 T132Y-P290Y 0.204631042478577 G164Y-T56G 0.203381518306877 P198Y-L45E 0.20257097259813 V25G-L52G 0.202439531020178 F11G-V69G 0.202207575117357 S63Y-V194Y 0.201455034168426 G57Y-N302Y 0.20123245567827 P79Y-L130Y 0.201198626768776 G127Y-A163Y 0.201056633666899 P35Y-G64Y 0.200818287629197 G54Y-I285Y 0.200673559381843 S71Y-P79Y 0.200014092354751 G31Y-D49E 0.199890573739155 I72Y-G60G 0.199847678481858 G127Y-V162Y 0.19954680718716 R174Y-V57G 0.199027004681833 P290Y-N302Y 0.198534907322887 Y80Y-Q161Y 0.197577287316965 Y28Y-R174Y 0.197338155750712 I59Y-G65Y 0.197196582644668 G119Y-V20G 0.196973394949387 R174Y-G183Y 0.196828486310543 F73Y-G191Y 0.195855993891523 G164Y-V15G 0.195676901037681 G76Y-V162Y 0.195368024627643 G183Y-G191Y 0.194842794785139 P273Y-V319Y 0.194342032724061 V8G-F63G 0.193485167783321 I59Y-G64Y 0.193170581536289 A166Y-T184Y 0.191823075825784 P37Y-P151Y 0.191822258384715 Q52Y-Q298Y 0.191803808770183 L297Y-N305Y 0.191325141419318 A190Y-F37E 0.190501973664633 Y28Y-A64G 0.190005828737336 Q282Y-N305Y 0.189326347022134 F33Y-G64Y 0.188447654364029 G38Y-Q161Y 0.18681323499279 Y21G-Y53G 0.186796431830445 T36Y-I9G 0.186280569393882 I175Y-T184Y 0.185983465738586 T307Y-L312Y 0.185675356933366 G57Y-P290Y 0.185216780995725 Y114Y-V23G 0.184969851785845 Q126Y-P284Y 0.184528990912302 F33Y-N66Y 0.183549403259529 M231Y-V271Y 0.183281449072715 M78Y-V162Y 0.183143308966195 G65Y-G164Y 0.183072046523777 V39Y-V157Y 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