[Wed Aug 24 18:30:01 2016] >Input file: TM5EKEATD.pdb >TM segments: A230-252_A265-283_C229-250_C265-282_D230-251_D265-282 MAIDEN score = -66.0624 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I270C-I270A 1.08938531850165 Q277C-L281A 1.02644072179006 I249C-V268A 0.918400825488407 F272C-L243D 0.863444039161298 L281C-Q277D 0.85368183643487 L242C-F272A 0.851750773705129 I270C-I270D 0.784011900885588 L242C-L271A 0.752339144880573 M267C-L266D 0.686829498469129 L271C-L243D 0.679800134317528 S265C-I250D 0.668525680262959 G235A-V276A 0.657062504530158 A245C-F272A 0.612938277759647 S235C-G282A 0.611325183072173 L281C-S280D 0.583050006949077 A246A-L251A 0.56301486025581 Y245A-I250A 0.558513730636425 A245C-M267A 0.543830264214412 L271C-A246D 0.542764872530384 L278C-Q277D 0.540192476361284 A244A-L249A 0.538228864100324 G282C-S236D 0.537216550363723 G275C-S242D 0.535302664967897 S265D-I270D 0.517732497513102 F272C-A246D 0.515925640548987 S241C-F272A 0.510785761249757 G282C-Y233D 0.485725376532185 S246C-V268A 0.474998141912781 W230C-S280C 0.466271342978877 Y233A-Q277A 0.454867026275939 V276C-S239D 0.454604789082969 G235D-V276D 0.445021033117913 W231A-S280A 0.444239497493997 W231D-S280D 0.441543333288931 S241C-G275A 0.43271843473472 G234C-I279A 0.429928381198272 Y232C-G282A 0.427900229920715 S238C-L278A 0.421482173754022 L278C-S236D 0.41592097623222 I270C-G274A 0.400723685449199 L266C-M267A 0.393049598255975 T231C-S280C 0.386956267428812 W231D-V276D 0.386496087202042 S235C-L278A 0.377364754183681 S280C-L281A 0.366440733872238 V268C-I250D 0.363314649277196 L273C-G275A 0.36122682720067 A245C-A269C 0.354981209036287 Y244C-G274A 0.350659023461341 A269C-L271A 0.340294161509691 G234C-V276C 0.338803801503533 F272C-S242D 0.334486977059748 I279C-S236D 0.333960719080993 L271C-S242D 0.332945798735903 Y244C-V276A 0.327334876772186 W230C-V276C 0.321472903435029 L281C-T232D 0.320351013442109 T232D-S280D 0.319736422664199 G235D-Q277D 0.317284324261923 V276C-Y245D 0.315829157164117 I270A-G275A 0.31548759566072 G275C-G275D 0.315051448043006 L242C-V276A 0.307799214291146 G275C-A269D 0.303710590428793 G235A-G282A 0.301695645240144 Y232C-Q277C 0.301213167373178 A244D-L249D 0.298155135352701 T232A-S280A 0.296863769402805 I249C-M267A 0.296488716841466 I270C-A269D 0.295931412239464 L273C-L271A 0.295910645414814 G274C-S236D 0.295892806795298 T231C-L281A 0.295513996024169 S238C-V276A 0.295249360256897 A269C-A269D 0.29097213929903 L278C-G275D 0.288131962321876 G234C-L281C 0.281875546155473 L271C-A269D 0.281769978252704 L266A-G274A 0.281332421509582 G234C-Q277C 0.278867233946524 G275C-S247D 0.277872198070614 S246C-G275A 0.277569111449631 A245C-L271A 0.275130924030904 G274C-G282C 0.272759757430459 G275C-L240D 0.271939225044794 L239C-G274A 0.270250582429656 S235C-I279A 0.269631821494302 G234C-L278A 0.269300120901157 I237D-S242D 0.267511869200547 G235D-G282D 0.26299580970061 G274D-G282D 0.258410796050311 S265D-L271D 0.258163443450407 L242C-G275A 0.256335640411238 G235D-L281D 0.256012176428834 L248C-V268A 0.254447597682159 L278C-S239D 0.250056291661382 G234C-G282C 0.249141303250801 Y233D-Q277D 0.241366364056372 L240D-Y245D 0.24099673782502 Y233A-V283A 0.239169252957971 L239C-Y244C 0.239148770454081 G235A-F272A 0.235728114264656 G274C-A246D 0.231788570115873 V276C-L243D 0.230702919491373 G274A-Q277D 0.230436355732721 G275C-Q277A 0.230108031006132 L240A-Y245A 0.227579019755127 S241C-S280A 0.227001767558912 M267C-V276C 0.226822795747367 G234C-F272C 0.226135916961894 S280C-S242D 0.225246551652788 A269C-F272A 0.225170531435244 S265C-S242D 0.225058980484659 Q277C-G275D 0.220399275313765 G274A-L273D 0.218931810959546 G274A-I270D 0.218913128301705 G235A-Q277A 0.218411180481858 Q277C-Y233D 0.215779344675303 S246C-L271A 0.215275479458095 Q277A-G274D 0.212522137121155 V268C-S265D 0.210029906560433 S280C-V283A 0.20989133357399 V276C-L240D 0.208815799773992 S235C-G274A 0.207350105554148 W231A-V283A 0.205345476954278 L239C-G275A 0.203529715795274 W230C-I279C 0.197427980011387 M267C-V268A 0.195983775907974 L278C-G274A 0.192553841859887 G275C-Y233D 0.189704624729894 L271A-V276A 0.187859782325855 L233C-V276C 0.187432454082746 G274A-G282A 0.187161415841635 A269D-G274D 0.18701001389465 S238C-M267A 0.186583852783355 I270C-L271A 0.186581058215895 L281C-G282D 0.185552942857008 V268C-M267D 0.18531632080739 A269A-G274A 0.185246568641162 S265C-Y245D 0.18345847188146 G235D-F272D 0.181380680361188 M267C-G274A 0.178365008658088 L281C-L281D 0.176603122387203 G274C-I270A 0.176376291763372 Y245D-S265D 0.176167557632653 G282C-L240D 0.175752348101742 L278C-G282A 0.175346167950213 G282C-I238D 0.174711735155475 I270A-G274D 0.174508422388245 G275C-L243D 0.17426941937511 S242A-M267A 0.17245637153035 I279C-G235D 0.171245410925653 L278C-G275A 0.170820814698927 L281A-S280D 0.1706170414542 Y232C-G275A 0.169634848755236 T231C-G275A 0.169016234718438 L266C-L266A 0.16857572987016 L278C-G235D 0.164527541414388 W230C-I279A 0.164284646909005 I270C-I250D 0.16302040029108 S265A-M267D 0.162486053116059 S242A-L266A 0.162475283114631 G235A-G275A 0.162159420028154 L281C-G274D 0.162040288541468 A246A-A269A 0.161592989093766 G274C-L273D 0.157141555528722 M267C-L251D 0.156820137167699 A269C-S242D 0.155925766024426 S235C-F272A 0.155659996897161 I279C-I237D 0.154558269920341 V268D-L273D 0.154499487621001 G234C-G274A 0.154385890659313 W231A-S239A 0.154174968452404 G282C-L281A 0.154069226694023 W230C-S238C 0.153844713774509 W231D-S239D 0.153745065295305 Y245A-S265A 0.152995973896553 I237C-G275A 0.152213960657199 S241C-L266C 0.151699243393809 G274C-G235D 0.151593618655546 V276C-G235D 0.15068417542421 A245C-I270C 0.149932097007174 F272C-A269D 0.149842890834929 I237C-V276C 0.148699774164887 G235A-L281A 0.148143528718853 G282C-G282A 0.147381684185406 A243C-I270A 0.146945265500976 G274C-I279C 0.146668033438042 G274C-L281A 0.145377034325002 S265C-M267D 0.144710491883624 V276C-I238D 0.144581659468022 S280A-L281D 0.144255722320436 L273C-Y245D 0.143199034214135 V230A-V276A 0.143099835817466 Y245D-L273D 0.142788288768033 M267C-S239D 0.141454480125796 G274C-L266D 0.141235261204081 G275C-T232D 0.140912530935268 L281C-L281A 0.140793259010973 I238D-V276D 0.14052100229433 S239D-G274D 0.140278556860342 S241C-V276A 0.139900989048204 Y233D-I279D 0.139861472640252 L271C-I237D 0.138770023047084 L234D-V276D 0.13874148774029 S238C-V283A 0.137066879813154 M267C-G274D 0.136609101595699 S265C-S265A 0.136322957912615 F272C-S236D 0.136188361205678 I237C-L271C 0.135553469853193 A246A-S265A 0.135454516250331 Y232C-Q277A 0.135433063642154 L273C-L241D 0.135354330969692 S241C-S265A 0.13496108396382 Y244C-L273A 0.134700189652527 V229C-V283A 0.134573786962304 L234A-L281A 0.134059154751573 G235D-G274D 0.13343125973093 I236C-I279A 0.133080826220581 G274C-L241D 0.132328245956638 L278C-I279A 0.132009340052276 L234D-L281D 0.131839315789219 S238C-G274C 0.13164533201564 L266C-L243D 0.13151987730849 F247C-S265A 0.131511515518528 L278C-I270D 0.130819723565422 V276C-S247D 0.130506674879437 G274C-M267D 0.130115193213468 V276C-L273D 0.129136730571541 G275C-L271A 0.128055225714365 L240C-V276A 0.127647797215619 I236C-Q277C 0.127201087885596 L234D-L278D 0.127099573816105 A269C-Q277C 0.12709773409387 G235A-I279A 0.126438987264029 A245C-G274A 0.125500855708355 I270C-L241D 0.125416134472637 I236C-V276A 0.125105409250271 G235A-S280A 0.124461539225023 I270C-G275D 0.124334211449952 S280A-Q277D 0.123249173485738 I249C-L273A 0.123192818813425 G274C-I237D 0.122990153530206 G234C-G274C 0.122486568438823 L234A-L278A 0.122407361249423 L278A-L281D 0.122374925597874 I270C-V268D 0.122078272898772 I270C-Q277D 0.122018408904647 I237D-Q277D 0.121491458886693 V268C-S239D 0.121343532525007 Y245A-L273A 0.120988507497532 G275C-F272D 0.12063771278752 I237A-Q277A 0.120592892227617 L266C-A269D 0.118866062559417 S241C-A269A 0.118836449042316 S238C-I279A 0.118539842395245 L248C-L266C 0.116817001238048 L240D-L249D 0.116763368130767 L240C-A269A 0.116441248645186 G282C-G282D 0.116211621090283 L271C-Y245D 0.116111950747417 L281C-S280A 0.11557168010748 S241C-I270C 0.115537321233579 S265C-L266A 0.115323464416471 S280C-L281D 0.115213544195749 G274C-L271D 0.115026688767356 L271C-S265D 0.1148521705158 L271C-L271D 0.114840889315064 S265C-V268A 0.114773220297437 I270C-A244D 0.114078576464635 S242A-I270A 0.113722091263777 Y245A-A269A 0.113693949550687 S265C-A244D 0.113432711505348 G275A-V283A 0.113112092238111 L271C-L249D 0.113038002823945 L266C-S265D 0.112614343409768 A269C-G275A 0.112243608682692 V268C-L251D 0.111877424987239 L239C-S280A 0.111528661529753 L241D-L273D 0.111523522156973 S280C-L278A 0.111339035453243 L243D-L273D 0.111237867193398 S265C-L266D 0.111225727790506 L273C-L266A 0.111066295189921 A269C-L241D 0.109934010163046 V276C-V276D 0.109042180675782 L281C-L240D 0.108918273798165 A269C-S265D 0.108855335363847 S235C-L273C 0.108750719277494 L233C-L278C 0.108606311960802 A269C-S265A 0.108557143640778 Y244C-L273C 0.108551202736695 V229C-G282C 0.10846553928076 A246D-S265D 0.107748879199103 G274C-M267A 0.107606715857668 L242C-I270C 0.107549606981563 Q277C-L271A 0.107289345522385 Q277A-S280D 0.106995436657555 G274C-A269A 0.106793154661417 V276C-S280A 0.106430948805964 L273C-I270A 0.106232424237003 S239A-G274A 0.106123901603716 A269C-L240D 0.105597384267555 G274C-V268D 0.105242344007206 Y245D-L251D 0.104930409567142 G275C-L234D 0.104368844773532 S265D-L273D 0.103692304870683 G274C-T232D 0.103380237478199 L240C-L278A 0.103155964847107 L266C-L249D 0.102572697210328 V276C-L273A 0.102029601295362 A269C-M267A 0.101476907215344 W231D-S236D 0.10092827846677 A269C-Y245D 0.100742712500234 L281A-Q277D 0.100568227636341 G234C-A269C 0.100452337598842 V230D-G282D 0.100033121088112 I236C-G274A 0.0993265966173893 S241C-L271C 0.0993045510656962 T232A-G282A 0.0990387278161582 I270A-I270D 0.0987419188739054 S241C-V268C 0.0986142558283323 L271C-L273D 0.098567604873673 S236A-L273A 0.0984716082345771 A246A-I270A 0.0981333696640512 L266C-V268D 0.0979641625118237 A245C-L273C 0.0979505155366286 L240C-L273A 0.0974513787505416 L240C-V276C 0.0971624410998226 L248C-A269A 0.0968540561767028 L240C-A245C 0.0964099698285459 L273C-A244D 0.0963569696570169 A245C-L278A 0.0961785861678628 L243A-L266A 0.0961489705887937 A269C-V276A 0.0958588945934298 V230A-G282A 0.0954457380562084 L233C-V283A 0.0951451644616927 L273A-Q277D 0.0946049133091083 A243C-G274A 0.0942536952409385 L243D-L251D 0.0928226243342092 V268C-L271A 0.0926360411070819 L233C-L281C 0.0925261204454131 L271A-L273D 0.0923691456008804 L273A-L271D 0.0916292606128342 Y233A-V276A 0.0911187255403965 L266A-M267D 0.0910707924690462 Q277A-L273D 0.0908283352982171 V229C-L278A 0.0907424404869137 F272C-L266A 0.0894547787508468 L239C-L281A 0.0891650715364469 Y233A-I279A 0.0889849262486191 A243C-I279A 0.0887599414486751 I279C-S239D 0.088723833853221 L271C-L273A 0.0882557139487146 L271C-L266A 0.0880012007805786 W231A-V276A 0.0874831426343086 T231C-G282C 0.0874444777171488 L241A-I270A 0.08711411177742 L273C-S247D 0.0870004324388676 T232D-G282D 0.0869883745261231 V276C-A246D 0.0867150198492164 L266C-Y245D 0.0866618183552648 L234A-V283A 0.0866444934139472 Q277A-L278D 0.0866162430805959 V268C-L240D 0.0863824937132041 I249C-L266C 0.0862915523598192 L240C-V268A 0.0855023379625166 I279C-W231D 0.0848637539514385 V268C-V268D 0.0843476403944239 I237A-I279A 0.0842597078913996 L240C-G274A 0.0836813749830023 I270C-S265D 0.0835769339243204 F247C-F272A 0.0831989962814457 L278C-L271D 0.0828172878408133 L271C-V276D 0.0823988707448269 L248C-L273A 0.0823151733039524 L243A-I270A 0.0822271043644323 S236D-L273D 0.081581697933057 Y245A-L266A 0.0810564046713242 A245C-I270A 0.0808626624788085 A269C-I270A 0.0808281317990853 V268C-L241D 0.0805070147823188 S235C-G274C 0.0804821987513797 L239C-V276C 0.08041803523491 L273C-I250D 0.0799120048527255 S242A-G275A 0.0798432502778734 G275C-G275A 0.0796172189435485 L248C-L266A 0.0795980895526878 L273A-G274D 0.0795944942808529 A243C-M267A 0.0790613510502529 I279C-Q277A 0.0787703741063228 A245C-S265C 0.0786681847847692 S239A-A269A 0.0780925137415975 L281C-V230D 0.0779559441618449 G235A-G274A 0.0773216586320065 I270C-Q277A 0.0772565168197556 S236D-G275D 0.0771151654974649 L266C-L271D 0.0770272511038161 L241A-L273A 0.0762012995014683 S242D-G275D 0.076184497862507 A246A-I270D 0.0761215068784989 F272C-L271D 0.0757126307487974 F272C-L266D 0.0752880305700994 L266D-G274D 0.074415206624741 I238A-I279A 0.0741008696252319 I238A-G275A 0.0739862228284744 S236A-G274A 0.073595235521098 A246D-L266D 0.0734643826086652 I236C-Q277A 0.0729770978768023 L273D-L281D 0.0729569016628284 S235C-G275C 0.0727302059962569 L273A-L278D 0.0715249135347564 G275C-I270A 0.0710884102478229 Q277C-I237D 0.0709646163048947 L278C-V230D 0.0705719337622792 S236D-G274D 0.0704821947224397 Q277C-I270A 0.0703905448182423 A243C-V276A 0.0695276739768871 M267A-M267D 0.0694780900144976 A269C-G274D 0.0692411948117799 I237C-G282A 0.0688386832689201 G274C-S280A 0.0687372514292237 Q277C-V283A 0.0685124707782334 L271A-L266D 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