[Mon Aug 29 17:07:27 2016] >Input file: TMA1A5B4S1G.pdb >TM segments: B193-206_B271-284_B335-350_B379-393_B421-434_B488-510_B538-554_B562-584_B600-614 MAIDEN score = -70.2129 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- I553B-A609B 2.0301000276395 P203B-P562B 1.06063319473445 W196B-F281B 0.914727267542872 C342B-L427B 0.914544166767211 V335B-R423B 0.881988109858929 T552B-A609B 0.871701509362975 L282B-E603B 0.840322344427529 L343B-H349B 0.790828805891015 L339B-T426B 0.77343552832123 V499B-R504B 0.766079549745404 W498B-L583B 0.758929104580291 H432B-I546B 0.732665392502353 W498B-C503B 0.702538601201713 M200B-L566B 0.691427392539235 Y548B-L602B 0.685024644851662 F425B-V601B 0.677932449741839 E540B-D576B 0.675579069708521 Y494B-L583B 0.672690441636455 V350B-T381B 0.668686702126795 L201B-L566B 0.660257053071821 A348B-F430B 0.656676236608223 I553B-V610B 0.65017520100433 Y494B-L575B 0.649149753291339 C342B-F430B 0.647972210336896 A204B-L566B 0.640569881044083 Y197B-L570B 0.626521294185197 I390B-F424B 0.620556259160328 L201B-P562B 0.607981114645379 V202B-W277B 0.587779637007404 P203B-L563B 0.563416657603562 F554B-A609B 0.560946571865251 M347B-L427B 0.558690207076572 I553B-L608B 0.554733994696417 M347B-F430B 0.537216933554293 A275B-V607B 0.519366180597551 T552B-V610B 0.509250937197657 F554B-A612B 0.495025998316844 M542B-Q547B 0.487357618744312 V571B-D576B 0.480481245012543 I553B-N613B 0.477061405535446 I578B-L583B 0.477043555573288 A204B-L563B 0.456913216585241 F429B-I546B 0.453508974525021 L343B-A348B 0.451566709184259 L343B-F430B 0.439481342921112 H386B-L427B 0.428216472429833 L339B-R423B 0.424364406784246 I553B-G606B 0.422324715722016 S489B-Y494B 0.421681584562967 Y197B-L566B 0.420916898163114 H432B-M544B 0.419265408527942 C491B-E543B 0.412660188568942 Q428B-Y548B 0.407388073421026 H349B-H386B 0.398475141300269 H386B-Q428B 0.393412641769163 A271B-V610B 0.390095593133844 C342B-R423B 0.389470851008616 E493B-T500B 0.37795625690466 Q428B-I546B 0.375589613671385 F429B-S434B 0.373454693757242 M274B-T551B 0.37249498039323 G346B-T389B 0.372046032873134 G549B-F554B 0.370836252983484 I553B-A612B 0.369151824374175 H432B-Q547B 0.365793896691332 I553B-I605B 0.364119407156972 Y548B-Q600B 0.36339185240821 W196B-N569B 0.363126928558602 W196B-P562B 0.362994613746606 N490B-L495B 0.356539499450163 V350B-Y387B 0.35610013308938 W196B-P203B 0.355048632547406 V202B-Y548B 0.354884430912125 W196B-M542B 0.354785182194671 I392B-R423B 0.352506963973324 C491B-T500B 0.351977631916942 L338B-R423B 0.351909685167612 W498B-K579B 0.351302032395139 P497B-L583B 0.348853414850792 A275B-T552B 0.347538193233361 Y548B-I605B 0.347292525024756 C342B-A348B 0.345764199532695 L338B-M393B 0.344950863910468 C491B-K502B 0.342728450159335 M347B-T431B 0.339967550980981 T279B-G606B 0.33941400802041 M347B-G382B 0.339376853773027 Q547B-E572B 0.336853847834408 L206B-F273B 0.335160954070988 G195B-Y548B 0.335007440175588 M545B-L602B 0.331658701368532 G382B-T431B 0.329486756674322 M200B-L563B 0.328899160596144 L282B-L602B 0.324656246417543 A271B-G606B 0.32223635127254 M545B-V601B 0.322023284690113 Y199B-F281B 0.317376406524994 F433B-F550B 0.314734798554488 P497B-Q584B 0.314526103708718 C491B-E540B 0.313194670708202 Y548B-V610B 0.312133194966968 E493B-D576B 0.311576096155557 I341B-G346B 0.305517153336072 E283B-Q600B 0.301942745986032 H501B-E510B 0.2998604292053 A275B-G549B 0.297628751956521 L201B-L563B 0.295674226090889 I345B-V385B 0.295215096422264 Y494B-D576B 0.292641143691564 C491B-V496B 0.291809986927212 M200B-Q547B 0.288154216500136 S489B-K579B 0.286303171258849 E572B-A577B 0.284644067683013 W196B-T279B 0.284609784656204 Y387B-R423B 0.282196459800674 Y494B-I578B 0.279990320876168 A205B-L563B 0.278110299280638 N490B-L575B 0.277901414429214 G549B-A565B 0.277074602490014 E283B-G606B 0.276631546909117 R423B-M542B 0.274791421951182 T421B-T426B 0.272068688623325 G606B-G614B 0.271320405638608 G346B-V385B 0.266911357598433 M200B-A565B 0.264418629867816 L338B-G346B 0.263887397825341 C342B-H432B 0.259211975444856 L343B-S434B 0.255233294842487 F554B-G606B 0.253593475620313 W277B-P562B 0.253110104911397 I345B-T389B 0.252758265975229 S489B-Q547B 0.25093481770861 P203B-T279B 0.248528924091148 N490B-K579B 0.246295346301852 G549B-Q600B 0.245925835485032 G549B-L602B 0.243874292918179 M340B-I345B 0.243660337500677 I422B-V601B 0.242973819557431 S489B-P497B 0.242613985799027 L282B-T552B 0.242250508057544 E493B-L583B 0.241317417685736 Q428B-F538B 0.240300826708617 H386B-H432B 0.239984211782367 V280B-Y548B 0.239664885204721 E493B-Q584B 0.239460059434997 N569B-L575B 0.237711360976413 T389B-T431B 0.235399192337335 A278B-T552B 0.235341031762392 T389B-T421B 0.233692376959972 Y199B-F273B 0.233595966750382 T198B-L563B 0.232430295604239 R574B-Q584B 0.231709725149605 P203B-Q547B 0.230414698831011 T279B-Q600B 0.230268651735305 T551B-L602B 0.227930060792428 T551B-L566B 0.224131428720876 F433B-A609B 0.223426942802521 G195B-L570B 0.223321129134538 T426B-T431B 0.223064966656537 Q428B-Q600B 0.222188221750161 G195B-L566B 0.218879559440209 M340B-R423B 0.21842126955671 H432B-T551B 0.218197587900626 P203B-L564B 0.217056396946155 M344B-V388B 0.214336996636607 I546B-G606B 0.213568650880792 T431B-Q547B 0.213291002844153 S489B-L495B 0.21196903711439 L343B-T426B 0.206634812309735 I341B-T389B 0.206509506215418 A278B-G606B 0.205997215942989 Q428B-F541B 0.205424144367133 V350B-G382B 0.204240425315792 A204B-F273B 0.204130985249408 W193B-I284B 0.203133199501478 L282B-M544B 0.202634762373185 M274B-L563B 0.202404362008023 Q547B-A565B 0.202376918728817 M340B-H349B 0.201139162197377 L343B-L427B 0.200848754445934 A275B-A609B 0.200660481026905 W498B-W582B 0.19915525815196 V496B-R504B 0.198312614853156 T431B-E543B 0.197716606289345 N490B-D576B 0.197589365191422 L194B-N569B 0.196448605832951 Y494B-C503B 0.196355538292686 V335B-F424B 0.195778443240493 P203B-G606B 0.194549618008719 C491B-C503B 0.194285455877054 S489B-V571B 0.1932734130294 K502B-W582B 0.191862585620418 L495B-C503B 0.191719035530985 T551B-T604B 0.189772529457989 M542B-Q600B 0.189679969147096 W193B-Y199B 0.189381353796399 V492B-P497B 0.188925267257815 E540B-Q547B 0.188238602866249 H501B-R507B 0.187757934326521 T279B-T551B 0.187656480394669 T500B-W582B 0.187583612057276 M274B-L602B 0.187480293521443 G346B-Q428B 0.187054209755664 Q547B-Q600B 0.186490019093982 G195B-P203B 0.186389749640287 Y197B-F541B 0.185839907237248 A275B-E283B 0.184737016621742 E540B-Y548B 0.183267692375028 F433B-N613B 0.183122519089073 V492B-W498B 0.182208959671334 G346B-M393B 0.181887958287341 Y199B-Q547B 0.18043461909684 T198B-W277B 0.18022365575559 T198B-A278B 0.179449166187093 H349B-G382B 0.178982352119605 R423B-D539B 0.178040392067573 G382B-H432B 0.177401323839829 N569B-D576B 0.177084852894678 F424B-V601B 0.176806085119069 Q547B-G606B 0.176399968214489 P203B-T551B 0.17517321868744 L566B-E572B 0.17353424021809 Y199B-A565B 0.173080619918944 T421B-T431B 0.172969616062243 L427B-M545B 0.172904626416166 A271B-G614B 0.172828753199847 H501B-K579B 0.172728704493503 A205B-T551B 0.172503055050795 V280B-E603B 0.172360861455957 V202B-L563B 0.170523418706155 T426B-G549B 0.170022823053979 M545B-D576B 0.168982763841469 V380B-V388B 0.168559952645183 F273B-G614B 0.16551047532263 E493B-H501B 0.165110844908898 T381B-H386B 0.164020929677791 Q547B-N569B 0.163450891360244 I422B-I605B 0.163076986804672 Y494B-E540B 0.162675168168589 I553B-I611B 0.162029809980856 T389B-I422B 0.162012324731459 A204B-G549B 0.161813585661883 N490B-I578B 0.161370178980598 Y548B-S568B 0.16124341048693 G195B-M545B 0.161151585294737 M347B-V385B 0.160899668928205 E543B-Y548B 0.159449654030234 S434B-N613B 0.158947197532995 W196B-L566B 0.157949348854693 P203B-F273B 0.157910777757097 T500B-L583B 0.156127731293738 A278B-F550B 0.154880921457599 F425B-S434B 0.154713316767534 V385B-I392B 0.154671621580018 Y199B-M544B 0.154605499651775 Q428B-F550B 0.15423585691054 I272B-V610B 0.153838357487121 D539B-N569B 0.153253922064388 M200B-I573B 0.152808006974422 T198B-P203B 0.152132359137148 W196B-L563B 0.151803350906447 V350B-H432B 0.151286393565177 L338B-T426B 0.151088019962301 F273B-T551B 0.15102382252267 A278B-I611B 0.150055741176055 I553B-G614B 0.14976424976743 I392B-T421B 0.149478542279994 I390B-T431B 0.148481286440406 T431B-G549B 0.147741387656064 T381B-I390B 0.147589906681554 K502B-R507B 0.147098406388267 W196B-T551B 0.146733232108957 T552B-L602B 0.145092129219259 W196B-T552B 0.144775118507551 Y494B-H501B 0.144428283627844 I341B-I390B 0.144015819730389 M274B-A612B 0.143856694004003 C491B-R574B 0.14379770692921 P497B-D576B 0.142627603170186 T421B-T604B 0.141397889532809 Y197B-I284B 0.141378632520552 Y197B-M542B 0.141343960189178 T279B-G549B 0.141062081166302 P203B-S568B 0.140893685236887 C342B-I422B 0.140021008031266 T426B-S434B 0.139503323096399 L488B-P497B 0.139375639015979 V499B-S505B 0.138846367760003 I605B-N613B 0.13869806021869 A278B-Q547B 0.138619083726992 S489B-N569B 0.138580030908853 L194B-I573B 0.138486762902934 Q428B-M544B 0.137813728820078 A275B-G606B 0.137192064235798 G549B-G614B 0.137073478287653 M274B-G549B 0.136894750669254 N490B-L583B 0.136840473643689 G606B-I611B 0.136754989580846 I272B-N613B 0.136719620039953 M544B-L575B 0.136627182494671 I578B-Q584B 0.135667893137645 I422B-E603B 0.135149787941877 A205B-M274B 0.134906458311722 I345B-A383B 0.134782684721849 F425B-E603B 0.13472965617485 T551B-I611B 0.134717423760613 M542B-N569B 0.134491787420104 E603B-V610B 0.133957906311905 L194B-I546B 0.133909334818407 A348B-A383B 0.133219576481453 Y199B-N569B 0.13195679307041 Y548B-N569B 0.131719857188715 E543B-R574B 0.131333840367506 L338B-I392B 0.130812915230544 W196B-S568B 0.130641751554555 T552B-I605B 0.130144191885565 T279B-I553B 0.129833081586918 A278B-N613B 0.12960271116585 T552B-N613B 0.129567295557474 A278B-A612B 0.12930207760439 L194B-E572B 0.129008925789257 T198B-T551B 0.12876342828541 E493B-N569B 0.128503142055591 T604B-N613B 0.128489938081425 T421B-I546B 0.127648339019794 L564B-V571B 0.127190864459485 W193B-L570B 0.126458812116998 Y199B-E283B 0.126097792056721 M274B-T552B 0.126078471568531 V380B-V385B 0.126076332955558 F281B-T552B 0.125376972919804 G195B-E572B 0.125164807870057 D539B-K579B 0.125146210388754 I391B-T421B 0.125004413447765 T426B-Q600B 0.124754578499232 M393B-F424B 0.123693165349062 L495B-M580B 0.123621807318718 Y197B-A205B 0.123352796762187 F430B-G549B 0.122880203245391 Q547B-A609B 0.122341905531374 S434B-Q547B 0.121249966983699 Y197B-R574B 0.12090005073124 E540B-I578B 0.120379902292132 V379B-V492B 0.120282819903782 R423B-F538B 0.120165628356772 L206B-L564B 0.12014690071691 F425B-G549B 0.119821877215503 N490B-M544B 0.119561327050364 W196B-L282B 0.119356169279237 G549B-P562B 0.11925175141189 C342B-I391B 0.118945123973421 C342B-H386B 0.118806131933191 M545B-L566B 0.118569854746511 L343B-M393B 0.118490978783978 E540B-Q584B 0.118220313530687 Y197B-A278B 0.118110417838505 L575B-Q584B 0.11788894701429 H432B-I553B 0.117882491566393 H432B-F541B 0.11725082695337 M344B-Y387B 0.117103861954325 L570B-A577B 0.116816772224869 E572B-K579B 0.116439296961512 E493B-I573B 0.116035749671754 L206B-L566B 0.11564805021861 H432B-A612B 0.114864843388561 M545B-I605B 0.114556184270953 H432B-Y548B 0.114199653122175 A205B-A271B 0.113618973017048 I345B-T431B 0.113062574955416 A204B-N569B 0.112969037454708 A275B-F554B 0.112861333628659 V335B-T389B 0.11252189984081 E493B-W498B 0.112510034174714 I392B-T426B 0.111953253113096 I345B-V388B 0.111693173855594 I345B-I391B 0.111220734808356 L194B-L201B 0.111033283730105 S489B-A577B 0.110792106248074 F429B-F541B 0.109892285920559 A348B-S434B 0.109752014425087 H501B-V581B 0.109615148614479 F425B-F541B 0.108929939099365 T552B-V601B 0.108822813157011 T337B-R423B 0.108682226391004 V388B-L427B 0.108128945420007 C491B-P497B 0.108051062937718 T500B-R507B 0.10783092535855 G195B-I573B 0.107805027300516 G346B-V379B 0.107447630583617 M344B-H349B 0.1071991275419 R504B-Q584B 0.107175110303272 C491B-A577B 0.10699953368872 G195B-A278B 0.106979005060127 G549B-V601B 0.106909822420338 A204B-Y548B 0.105998527390736 A205B-A565B 0.105989900987415 A204B-L564B 0.105867609239139 V496B-D576B 0.105759267781967 L339B-A348B 0.105564289288568 T500B-K579B 0.105017206613934 A348B-V388B 0.10477374851479 H349B-T431B 0.104235458157937 P562B-A609B 0.103354077152471 F429B-G606B 0.103092631384738 L488B-L575B 0.102666697136346 F281B-G549B 0.102473680485866 V388B-T431B 0.102380673239444 V335B-I341B 0.10161965437551 F433B-T551B 0.101595936867509 W193B-F281B 0.101210434477512 L563B-V571B 0.101011963045938 A348B-T381B 0.100715911653019 Y197B-E543B 0.099259291475235 G346B-T426B 0.0992179700734191 L338B-V385B 0.0990197419322865 V496B-L575B 0.098446018229239 E493B-R574B 0.0984227658981832 V202B-T551B 0.0983578879589304 L276B-L608B 0.097757514058189 F273B-A609B 0.0974636194254428 T421B-E540B 0.0969170441951333 A348B-V379B 0.096867458076483 C491B-H501B 0.0968189499097978 A278B-L608B 0.096201943321379 T198B-E283B 0.0958794491418096 V202B-L564B 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