[Mon Aug 29 17:21:40 2016] >Input file: TMA1ZBD6S2G.pdb >TM segments: B221-229_B240-246_B248-256_B262-270_B280-285_B295-301 MAIDEN score = 51.1964 Below is the ranking list of the residue pairs and their related pseudo-energy (the lower, the better). This includes inter-chain residue pairs. The maximum (least favorable) pairwise energy value has been set to 10. See the MAIDEN software article: Postic,G., Ghouzam,Y. and Gelly,J.C. (2015) Biochimie, 115, 155–161. ----------- ---------------- Calpha pair Empirical energy ----------- ---------------- V242B-V284B 10 K243B-H252B 10 K245B-L250B 10 S225B-V264B 6.20428384317847 K224B-D266B 3.97322254476057 V246B-I280B 2.07841982719737 F285B-M296B 1.55206411367116 I281B-K300B 1.44971496338539 V284B-M296B 1.37482172341255 F285B-F295B 1.35921166320043 I280B-L301B 1.27968064184274 F244B-K283B 1.19894794634344 K243B-K253B 1.03687434036509 V246B-I281B 1.02822907167738 H222B-V268B 1.02192498354743 V221B-F269B 0.976427909808926 V284B-F295B 0.943222280354893 V242B-F285B 0.901733189452696 F244B-L251B 0.885436710413249 F244B-L250B 0.879777067659345 I281B-L301B 0.832168361259302 V221B-I270B 0.798401066882726 L223B-E267B 0.786578756092847 Y241B-T256B 0.75675389685691 V242B-K253B 0.731626174124146 V282B-A299B 0.710537193551129 K283B-F295B 0.704657029878263 K283B-M296B 0.673481574307518 V242B-R255B 0.667911184612998 L223B-W265B 0.667397778974717 F244B-I281B 0.656547725699536 V221B-V268B 0.63841678770261 I281B-S298B 0.637766238205278 K243B-K283B 0.632172289684718 L223B-V268B 0.622399759043287 K283B-A299B 0.599430194339519 F244B-V282B 0.598850658678724 K224B-V268B 0.572899275121152 F285B-G297B 0.567950508556041 L223B-F269B 0.553285432497986 P240B-F285B 0.544693859247066 K245B-I281B 0.544249371301786 L251B-F269B 0.527351343770306 V221B-E267B 0.512856148402673 R249B-I270B 0.497062660478017 K243B-I281B 0.490302936325116 S254B-W265B 0.489066071807315 L223B-S254B 0.456077441750549 F244B-I280B 0.448850049667305 K243B-L250B 0.448608442242122 T256B-E267B 0.425673156657279 K245B-H252B 0.423519509047158 K243B-F285B 0.418913749465261 Y241B-K253B 0.413828932317893 P240B-N262B 0.369144275683961 P240B-V284B 0.364730241383505 P240B-R255B 0.33602261902163 P240B-K283B 0.329514317987071 K243B-R249B 0.306096272480094 K243B-R255B 0.305330407609235 H252B-K283B 0.303022289359653 V242B-L251B 0.301230503865352 L229B-M296B 0.30027550596771 K224B-N262B 0.299442927081625 I280B-A299B 0.297729723186235 S227B-P263B 0.284501427100955 S225B-K283B 0.284422148591136 I281B-A299B 0.275964845912766 N262B-E267B 0.274477189111998 K253B-E267B 0.272921229896108 G226B-D266B 0.258752095470665 Y241B-K283B 0.257963862236805 Y241B-D266B 0.253338243012151 Y241B-V268B 0.251901232124426 T256B-P263B 0.237946880866212 P240B-S298B 0.235367448643039 P240B-H252B 0.226451779898325 H222B-K243B 0.225180621326449 F244B-H252B 0.221440665555765 S225B-K243B 0.219345685539544 V221B-I281B 0.218741089463031 V284B-S298B 0.213751386581245 S227B-G297B 0.211242413671706 H222B-F269B 0.207425054606125 V282B-F295B 0.206573173153022 V282B-K300B 0.204710268950939 R255B-V268B 0.199363163229984 L251B-V282B 0.193534782198378 S225B-V268B 0.191459312280092 P263B-G297B 0.190191872966643 S225B-S298B 0.186521195756844 V221B-H252B 0.184781358676164 S227B-Y241B 0.18313713946432 L229B-K283B 0.182587507857495 H222B-V282B 0.181803281179134 H252B-I281B 0.181646936022859 S225B-V284B 0.179734069313076 H222B-K253B 0.175989225728839 H222B-V242B 0.173377994730793 N262B-F285B 0.173288358643125 T256B-D266B 0.172613221643345 R249B-V282B 0.168779495814333 H222B-K245B 0.167768443014782 S254B-V282B 0.16627191075156 T256B-K283B 0.166006292287955 H222B-K283B 0.160680891107999 F244B-R249B 0.160073696972447 L251B-W265B 0.159752474449071 K243B-V284B 0.1582280693979 I281B-G297B 0.150946090002318 S227B-W265B 0.150639036805077 G226B-K283B 0.150413389945081 V221B-S254B 0.149403803788967 L229B-V284B 0.144300970860249 K243B-G248B 0.142913365069928 K224B-T256B 0.142226644921382 V242B-S298B 0.140789059441647 V264B-V284B 0.137602878234793 G226B-S298B 0.136777954071432 Y241B-N262B 0.134395841591381 H222B-H252B 0.133202961789594 K253B-F285B 0.132699362824335 K224B-F244B 0.132466033089828 S225B-R255B 0.129791926553923 R255B-V284B 0.128886886235986 L223B-V284B 0.127734248044907 K224B-V284B 0.127202339344513 K245B-V268B 0.127199038275712 V221B-K253B 0.127138468632491 V242B-W265B 0.126794060834071 G226B-T256B 0.126538794745777 K224B-V282B 0.126305108152075 S254B-N262B 0.125764506095049 Y241B-L250B 0.123354874749834 S225B-V282B 0.123226944126365 G226B-F285B 0.123115869587925 V242B-K300B 0.121804476477974 P240B-K253B 0.120573323909221 G226B-R255B 0.119426540070376 L223B-N262B 0.116656771936004 S227B-V284B 0.115547464855241 V221B-V264B 0.113248608818847 H252B-W265B 0.112962131626021 G248B-I270B 0.112305018388772 F244B-F295B 0.110752483653409 H222B-A299B 0.109008094102826 H222B-V264B 0.108983200663254 S254B-E267B 0.107862192688773 D228B-V284B 0.103402041329586 S227B-V264B 0.102598539567677 H252B-V284B 0.101773725439083 L229B-Y241B 0.101659474326602 V242B-V282B 0.100720897851004 T256B-V282B 0.100255066222694 K245B-S254B 0.100220243241599 L223B-K245B 0.0986175640605724 V242B-S254B 0.0964296076249926 L229B-S298B 0.0955339619005775 F244B-F285B 0.0943646675523531 V246B-V282B 0.093556761776 K243B-D266B 0.0926761432090956 H252B-V282B 0.0917497887579609 K224B-K243B 0.0915609844044898 L223B-V246B 0.0914683729535819 L223B-L250B 0.0906894441436872 V242B-I280B 0.0900649841784035 D228B-P240B 0.087256712073766 K243B-G297B 0.0869016586121256 K224B-K253B 0.0861690480826098 S225B-F244B 0.0843490979421802 L250B-F285B 0.0840919416591266 H222B-L251B 0.0828737339162986 I281B-M296B 0.0787711989033344 L250B-V268B 0.0764290565849617 F244B-D266B 0.0753047527813584 L250B-V284B 0.0747665657681232 P240B-G297B 0.0720826649479789 K243B-W265B 0.0709547729064957 V242B-N262B 0.0707612079796739 D228B-F285B 0.0704071739447818 S254B-V268B 0.0697719172231723 F295B-K300B 0.0693883396048829 H252B-S298B 0.0689430700469963 L223B-T256B 0.0680962341728223 L229B-A299B 0.0664112276668815 Y241B-S298B 0.0658571636302847 Y241B-E267B 0.0642941601218763 F244B-E267B 0.0640073974285409 V221B-R249B 0.063098843026562 Y241B-H252B 0.0628491247612764 P240B-D266B 0.060737729034535 G226B-E267B 0.0602015381101282 R255B-K283B 0.0598135731265366 K283B-L301B 0.0557170654279071 K253B-D266B 0.0541795833800376 K243B-V268B 0.0530680938135759 W265B-K283B 0.0526143978432554 K243B-F269B 0.0519165410862625 S227B-D266B 0.0507529640997976 P263B-V284B 0.0506975847239883 K245B-V282B 0.0484450978936937 Y241B-F285B 0.0480811602282465 P263B-M296B 0.0480282382891269 F244B-M296B 0.0464936836665883 H252B-I280B 0.046246266369544 R255B-F269B 0.0453766108282831 L251B-F285B 0.0446043539312191 G226B-W265B 0.0445259074450757 L229B-N262B 0.0442227539115612 S225B-M296B 0.0429948283707045 K243B-T256B 0.0409493896553643 V246B-H252B 0.0354765094751373 K253B-V282B 0.0347419762159747 P240B-L251B 0.0340902735361528 L223B-P263B 0.0337941573765654 K253B-V284B 0.0330554804007087 V246B-V268B 0.0323554150130576 P240B-W265B 0.0322877728370234 V221B-K243B 0.0322481597117075 V284B-K300B 0.0320050547495707 V246B-K283B 0.0318774378816875 G226B-S254B 0.0317166529019487 V268B-V282B 0.0305540443980115 S227B-V242B 0.0297497456129157 Y241B-W265B 0.0292802989528669 H252B-E267B 0.0288938415238951 P240B-V282B 0.0280567193785319 L250B-A299B 0.0273948612576422 S227B-S298B 0.0271658476590626 S225B-T256B 0.0265541790949788 D228B-M296B 0.0264316052179003 H222B-I280B 0.0255554880135509 L223B-G297B 0.0255269794031727 P240B-A299B 0.024726857787869 G226B-V264B 0.0243347904280276 K245B-V284B 0.0241758484808163 K245B-K300B 0.0237120749796933 S225B-K253B 0.023229131898443 K224B-E267B 0.0229846878935351 P263B-F285B 0.02150623389735 L250B-R255B 0.0206324044045187 D228B-F295B 0.0204139084753434 V284B-L301B 0.0199651062948665 S254B-K283B 0.0194453662540188 L251B-I270B 0.0183777301959402 K253B-I281B 0.0182802350377542 H252B-V268B 0.0172920437837356 V242B-H252B 0.0163161670840957 H252B-G297B 0.0153916769371749 T256B-V268B 0.0145635214642859 S225B-F285B 0.014555714228333 R255B-F285B 0.0123332748956 S227B-K283B 0.0118039635356122 H252B-I270B 0.0116015171752173 D228B-S298B 0.0100483596552283 K253B-I270B 0.00813000654073459 L223B-A299B 0.00727041082314628 D228B-G297B 0.00674088116008642 F269B-V282B 0.00532107727065934 K245B-L251B 0.00454222280219518 T256B-N262B 0.0019819105589729 G226B-K243B 0.000816637564735156 F244B-W265B 0.000490509908164682 H222B-Y241B 0.000306974615085664 L223B-K283B -0.00188310032103534 P240B-M296B -0.00361186064377073 F244B-K253B -0.00402963768162756 N262B-M296B -0.00431225476411701 F285B-S298B -0.00478339351861923 F244B-R255B -0.00633529477686312 S225B-H252B -0.00783250823703163 K245B-S298B -0.00807404852350779 Y241B-I281B -0.00879126498090876 S225B-G297B -0.0106843208012724 R249B-I280B -0.0118666375421945 T256B-M296B -0.0128233467162557 L223B-L301B -0.0137024532190733 S227B-F285B -0.0140696377459228 G226B-G297B -0.0140880722941423 H222B-I281B -0.0178898360093696 G226B-A299B -0.0197607516815135 G248B-V282B -0.0198205849074524 K243B-V264B -0.0202433173021088 V246B-S254B -0.020358984962574 S227B-T256B -0.021146369987866 V221B-K300B -0.0214320556547074 P240B-V264B -0.022607845376303 V246B-A299B -0.0232628904074563 K245B-E267B -0.0251149429513278 P263B-V268B -0.025363827486081 H252B-V264B -0.0277251550009749 R255B-N262B -0.02872205420277 V221B-A299B -0.0300251465531166 T256B-F285B -0.030468388648545 S254B-I281B -0.0332874862967436 Y241B-P263B -0.0352571726934969 L250B-S298B -0.03674418213126 H252B-A299B -0.0375168226985494 F269B-I281B -0.0379559183356868 R255B-E267B -0.0394883516602589 I281B-F295B -0.0398610425078072 S225B-N262B -0.0407282600332486 K243B-E267B -0.0411776030419082 V284B-A299B -0.0425747931419549 R249B-S254B -0.045052065299538 Y241B-A299B -0.0452344407859169 L229B-V282B -0.0462761228612711 L251B-T256B -0.0469225952549257 S254B-D266B -0.0480902756607917 L251B-K300B -0.0487378358489444 H222B-V246B -0.0498610030013928 H252B-M296B -0.0505120258081404 F244B-G297B -0.052651246158667 L223B-H252B -0.0528379299609902 V242B-F269B -0.0530349040995612 V242B-T256B -0.0531270851307086 V246B-I270B -0.056213682461447 K224B-S254B -0.0581421436854116 V242B-V264B -0.0585659901447553 H222B-L250B -0.0597079336633013 V242B-R249B -0.0598889784817773 L223B-Y241B -0.0614499534194031 K283B-G297B -0.0662957049270148 F244B-T256B -0.0664839693152333 H222B-S254B -0.0665292052616273 R249B-K283B -0.0708477445461893 R255B-W265B -0.0711958068505347 K243B-S298B -0.0713593601478253 K245B-K253B -0.0714322327384833 V221B-G226B -0.0721235972034151 H222B-V284B -0.0722542033457634 F269B-I280B -0.0725403499499753 L229B-T256B -0.0733210995967903 W265B-F285B -0.0733366087455725 S225B-F269B -0.0763650361889556 L251B-A299B -0.0769181309628486 L251B-I280B -0.077427696736093 L223B-P240B -0.0774412874341763 D228B-V264B -0.0781217345729006 V221B-Y241B -0.078666335287465 G226B-V268B -0.0792095976216816 S227B-M296B -0.0795401656925125 V242B-V268B -0.0809578023751752 N262B-V284B -0.0828130546169865 H222B-F244B -0.0848973250229523 K224B-V242B -0.0850011697996456 T256B-F295B -0.0850295839152198 L251B-D266B -0.085679469173659 T256B-W265B -0.085888220927187 S254B-I270B -0.0891917322209224 V242B-M296B -0.0903954768394941 L223B-S298B -0.0909390257127312 F244B-L301B -0.0918694514690983 K224B-H252B -0.0932958680398401 K224B-I270B -0.0933806321148603 F285B-A299B -0.0936586909894124 S225B-Y241B -0.094754874432823 V242B-P263B -0.0950469560106484 K224B-V264B -0.0956470256983621 R249B-V268B -0.0963299274315412 V221B-V242B -0.0971264756407603 K245B-G297B -0.0980004267795733 F244B-S298B -0.0981318608230596 L251B-V284B -0.0986403474927622 V242B-E267B -0.100128116307511 V242B-K283B -0.10072332617485 H252B-F295B -0.101783131769364 V246B-S298B -0.102560048316965 S225B-A299B -0.103290853217519 L223B-I270B -0.104192215677121 I280B-G297B -0.105756270755667 Y241B-M296B -0.111952864007264 S227B-P240B -0.114670123684326 V242B-D266B -0.114782009403875 K243B-P263B -0.114825595868346 G226B-M296B -0.115183449841413 G226B-F295B -0.116260928462011 G226B-F244B -0.116607238239181 V268B-I280B -0.116693829175493 V264B-F285B -0.117612422243668 G248B-K283B -0.118568551996049 K253B-V264B -0.120798646467133 D228B-W265B -0.120830335136812 K243B-F295B -0.122858609957708 S227B-A299B -0.124857185543465 K243B-I270B -0.124938290809041 G226B-V284B -0.125594711534669 L250B-E267B -0.125640234602276 L223B-F285B -0.12613141685683 V221B-I280B -0.127615016129177 W265B-V284B -0.12914151897552 L223B-D266B -0.129448842080442 L223B-I280B -0.130214110137735 K224B-R255B -0.130280687156367 G248B-F269B -0.130335195717219 K253B-K283B -0.130439163803939 G248B-K300B -0.130807519650128 I270B-V282B -0.131830939980638 V221B-W265B -0.131944765692598 V242B-A299B -0.134196084798305 H222B-E267B -0.135331949804153 I270B-I281B -0.137403334384842 G226B-V282B -0.137902360195819 K253B-F269B -0.139567058457818 V242B-G297B -0.139781475277129 K224B-K283B -0.139900727690049 L251B-S298B -0.141401521910597 S225B-E267B -0.142018794539407 V221B-V282B -0.143921839297592 L229B-F285B -0.144151745485035 K243B-S254B -0.144463383498494 Y241B-G297B -0.145820471883837 G226B-P240B -0.147028841759293 F244B-V268B -0.14742004908884 L250B-I281B -0.147565229113516 L223B-F244B -0.147781597747471 K253B-P263B 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