40S ribosomal protein S29 (RPS29)

The protein contains 56 amino acids for an estimated molecular weight of 6677 Da.

 

No function (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 100%
Model score: 100
No model available.

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VariantDescription
DBA13
DBA13

The reference OMIM entry for this protein is 603633

Ribosomal protein s29; rps29

DESCRIPTION

The RPS29 gene encodes a component of the small 40S ribosomal subunit and is essential for rRNA processing and ribosomal biogenesis (summary by Mirabello et al., 2014).

CLONING

By searching sequence databases with the partial sequences of randomly selected cDNAs from a human colorectal cDNA library, Frigerio et al. (1995) identified cDNAs encoding homologs of rat ribosomal proteins S5 (RPS5; 603630), S9 (RPS9; 603631), S10 (RPS10; 603632), S29 (RPS29), L5 (RPL5; 603634), L21 (RPL21; 603636), L27a (RPL27A; 603637), and L28 (RPL28; 603638). Frigerio et al. (1995) completed the cDNA sequences of these human ribosomal proteins. The deduced 56-amino acid human RPS29 is identical to rat Rps29. Northern blot analysis suggested variable expression of RPS29 in colorectal cancers compared to adjacent normal tissues, although no correlation between the level of expression and the severity of the disease was found. Kondoh et al. (1996) isolated RPS29 cDNAs by differential hybridization screening of a human colon carcinoma cDNA library with probes derived from a colon carcinoma cell line (HT29) and a differentiated subclone (C III). Northern blot analysis detected higher levels of the 0.3-kb RPS29 transcript in the undifferentiated HT29 cells compared to the differentiated C III cells, and in growth-arrested HT29 cells compared to rapidly proliferating HT29 cells. RPS29 has a zinc finger-like domain that can bind zinc. The authors demonstrated that RPS29 can enhance the tumor suppressor activity of KREV1 (179520).

MAPPING

By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPS29 gene to chromosome 14q.

MOLECULAR GENETICS

In affected members of 2 unrelated families with Diamond-Blackfan anemia-13 (DBA13; 615909), Mirabello et al. (2014) identified heterozygous missense mutations in the RPS29 gene (603633.0001 and 603633.0002). The mutations, which were found by whole-exome sequencing, segregated with the disorder in the families; however, both families showed evidence of incomplete penetrance. Functional studies showed that the mutations caused haploinsufficiency of RPS29. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 603633 was added.