ADP-ribosylation factor 5 (ARF5)
The protein contains 180 amino acids for an estimated molecular weight of 20530 Da.
GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.', '(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. (updated: Dec. 5, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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Biological Process
Golgi to plasma membrane transport
Intracellular protein transport
Metabolic process
Protein transport
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
Small GTPase mediated signal transduction
Vesicle-mediated transport
Cellular Component
Cytoplasm
Extracellular exosome
Golgi apparatus
Perinuclear region of cytoplasm
Plasma membrane
The reference OMIM entry for this protein is 103188
Adp-ribosylation factor 5; arf5
DESCRIPTION
ADP-ribosylation factors (ARFs) are guanine nucleotide-binding proteins, approximately 20 kD in size, that serve as GTP-dependent allosteric activators of cholera toxin ADP-ribosyltransferase activity (summary by Tsuchiya et al., 1991).CLONING
To the 4 species of mammalian ARF, termed ARF1-4, previously identified by cloning, Tsuchiya et al. (1991) added new ARF-like genes, ARF5 and 6 (600464), encoding proteins of 180 and 175 amino acids, respectively. Both proteins contain consensus sequences believed to be involved in guanine nucleotide binding and GTP hydrolysis. ARF5 was more similar in deduced amino acid sequence to ARF4, which also has 180 amino acids.GENE STRUCTURE
McGuire et al. (1997) determined that the ARF5 gene contains 6 coding exons spanning approximately 3.2 kb of genomic DNA.MAPPING
McGuire et al. (1997) mapped the ARF5 gene to a YAC contig on chromosome 7q31.3. ... More on the omim web site
Dec. 9, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 103188 was added.
Jan. 27, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed