Syntaxin-7 (STX7)

The protein contains 261 amino acids for an estimated molecular weight of 29816 Da.

 

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  5. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  6. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  7. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 42%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 603217

Syntaxin 7; stx7

CLONING

In the cell, the specificity of vesicle transport is thought to occur through the interaction of vesicle proteins with receptors such as syntaxins on a particular target membrane. See alpha-SNAP (603215). Pep12p, or Vps6p, is a syntaxin identified in S. cerevisiae and in Arabidopsis that is required for the sorting of soluble hydrolases from the Golgi complex to the vacuole. By searching an EST database for human homologs of Pep12p, Wang et al. (1997) identified partial syntaxin-7 (STX7) cDNAs. Using a partial cDNA as a probe, they isolated a fetal brain cDNA corresponding to the entire coding region of syntaxin-7. The deduced 261-amino acid STX7 protein contains a putative C-terminal transmembrane domain and 3 coiled-coil domains. The human protein shares 34% and 25% sequence identity with Arabidopsis and yeast Pep12p, respectively. In vitro, recombinant protein bound specifically to alpha-SNAP, a key regulator of transport vesicle fusion at multiple stages of the secretory pathway. Northern blot analysis revealed that syntaxin-7 was expressed as 1.8- and 3.6-kb mRNAs in all tissues tested.

GENE FUNCTION

Using immunocytochemistry, fluorescence, and confocal microscopy, Collins et al. (2002) showed that STX7 and STX13 (STX12; 606892) are involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. STX12 is localized to the recycling endosome compartment, whereas STX7 is found in late endosomes and lysosomes and both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

MAPPING

By analysis of a human monochromosomal panel, Wang et al. (1997) mapped the STX7 gene to chromosome 6. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603217 was added.