Protocadherin-1 (PCDH1)
The protein contains 1060 amino acids for an estimated molecular weight of 114743 Da.
May be involved in cell-cell interaction processes and in cell adhesion. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs12517385 | |
| dbSNP:rs12515587 | |
| dbSNP:rs3822357 |
Biological Process
Cell adhesion
Cell-cell signaling
Homophilic cell adhesion via plasma membrane adhesion molecules
Nervous system development
The reference OMIM entry for this protein is 603626
Protocadherin 1; pcdh1
Pc42
CLONING
Cadherins are a group of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. See 601120. By PCR of rat, mouse, and human neural cell cDNA with degenerate primers based on conserved regions of the cadherin extracellular domain, Sano et al. (1993) isolated partial cDNAs encoding pc42 and pc43 (PCDH2; 603627). They used the partial cDNAs to screen a human brain library and recovered cDNAs corresponding to the entire coding sequence of both genes. Like typical cadherins, both pc42 and pc43 contain an N-terminal extracellular domain, a transmembrane domain, and a C-terminal cytoplasmic region. The extracellular domains of pc42 and pc43 contain 7 and 6 repeats, respectively, of a subdomain that is very similar to those of cadherins, but with characteristic properties. The cytoplasmic domains of pc42 and pc43 share no homology with those of cadherins. The authors isolated partial cDNAs encoding proteins with the protocadherin repeats from Xenopus, Drosophila, and C. elegans. Since pc42 and pc43 are more closely related to one another than to classic cadherins, and because of the existence of similar proteins in distantly related organisms, Sano et al. (1993) suggested the name 'protocadherins' for this family of proteins. Sano et al. (1993) reported that pc42 migrated at 170 kD on Western blots of extracts of mammalian cells expressing the protein. Immunofluorescence microscopy localized pc42 to the periphery of cells, primarily in cell-cell contact sites. Cells expressing pc42 showed cell aggregation activity.MAPPING
By analysis of somatic cell hybrids, Del Mastro et al. (1995) mapped the pc42 gene to 5q32-q33. Sago et al. (1995) noted that the mouse Pcdh1, Pcdh2, and Pcdh3 genes form a cluster on chromosome 18. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for PCDH1
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603626 was added.