Secernin-1 (SCRN1)
The protein contains 414 amino acids for an estimated molecular weight of 46382 Da.
Regulates exocytosis in mast cells. Increases both the extent of secretion and the sensitivity of mast cells to stimulation with calcium (By similarity). (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs35960711 | |
| dbSNP:rs17324153 |
The reference OMIM entry for this protein is 614965
Secernin 1; scrn1
Ses1
Kiaa0193
CLONING
By sequencing clones obtained from a size-fractionated KG-1 myeloid cell line cDNA library, Nagase et al. (1996) obtained a partial SCRN1 clone, which they designated KIAA0193. Northern blot analysis detected SCRN1 expression in all human tissues and cell lines examined, with highest expression in brain. By database analysis to identify orthologs of mouse secernin-1 (named after secern, an archaic English term for secrete), Way et al. (2002) identified human SCRN1, SCRN2 (614966), and SCRN3 (614967). The deduced SCRN1 protein contains 346 amino acids and is N-terminally truncated compared with mouse Scrn1.GENE FUNCTION
Way et al. (2002) found that purified bovine brain secernin-1 stimulated secretion of hexosaminidase from permeabilized rat mast cells.MAPPING
By radiation hybrid analysis, Nagase et al. (1996) mapped the SCRN1 gene to chromosome 7. Way et al. (2002) mapped the SCRN1 gene to chromosome 7p14.3-p14.1 by genomic sequence analysis. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 614965 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed