Dynactin subunit 2 (DCTN2)
The protein contains 401 amino acids for an estimated molecular weight of 44231 Da.
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II
Cell population proliferation
Ciliary basal body-plasma membrane docking
Endoplasmic reticulum to Golgi vesicle-mediated transport
G2/M transition of mitotic cell cycle
Melanosome transport
Mitotic cell cycle
Mitotic metaphase plate congression
Mitotic nuclear division
Mitotic spindle organization
Organelle organization
Protein localization to centrosome
Regulation of G2/M transition of mitotic cell cycle
The reference OMIM entry for this protein is 607376
Dynactin 2; dctn2
Dynamitin
Dynactin complex, 50-kd subunit; dctn50
DESCRIPTION
DCTN2 is a 50-kD subunit of dynactin, a multiprotein complex associated with dynein. Cytoplasmic dynein is a molecular motor responsible for minus-end-directed movement along microtubules. See DCTN1 (601143) and DNCH1 (600112) for additional information about the dynactin complex and dynein.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the DCTN2 gene to chromosome 12 (TMAP RH70660).ANIMAL MODEL
LaMonte et al. (2002) generated a mouse model of amyotrophic lateral sclerosis (ALS; 105400) by overexpressing dynamitin in postnatal motor neurons of transgenic mice. They found that dynamitin overexpression disrupted the dynein-dynactin complex, resulting in an inhibition of retrograde axonal transport. The authors observed a late-onset, slowly progressive motor neuron degenerative disease in the transgenic mice and concluded that their mouse model confirms the critical role of disrupted axonal transport in the pathogenesis of motor neuron degenerative disease. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607376 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed