Basal cell adhesion molecule (BCAM)
The protein contains 628 amino acids for an estimated molecular weight of 67405 Da.
Laminin alpha-5 receptor. May mediate intracellular signaling. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.
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The reference OMIM entry for this protein is 111200
Blood group--lutheran system; lu auberger system, included; au, included
A number sign (#) is used with this entry because the Lutheran (Lu) and Auberger (Au) blood group antigen systems are both encoded by the BCAM gene (612773).
DESCRIPTION
Lutheran is a complex blood group system consisting of 19 antigens numbered from LU1 to LU21, with 2 numbers (LU10 and LU15) declared obsolete. Four pairs of these antigens have allelic relationships: LU1/LU2, also known as Lu(a)/(b); LU6 and LU9; LU8 and LU14; and LU18/LU19, also known as Au(a)/(b) (Karamatic Crew et al., 2007). Although the Au(a) antigen was found by Salmon et al. (1961), the antithetical antigen, Au(b), was not found until 1989 (Frandson et al., 1989). Daniels et al. (1991) showed that the Au(a) and Au(b) antigens belong to the Lutheran system. For a discussion of Lutheran blood group phenotypes, see 247420.MOLECULAR GENETICS
- Lutheran System El Nemer et al. (1997) found that BCAM coding sequences amplified from the genomic DNA of Lu (a+b-) or Lu (a-b+) blood donors showed a single G-to-A transition in the gene (H77R; 612773.0001). When expressed in Chinese hamster ovary (CHO) cells, Lu cDNAs carrying 229A or 229G produced cell surface proteins that reacted with anti-Lu(a) or anti-Lu(b) antibodies, respectively, showing that these nucleotides specified the 2 major alleles of the Lutheran blood group locus. Parsons et al. (1997) also demonstrated that the Lu(a)/Lu(b) variant is determined by an H77R variant in the BCAM1 gene, resulting in a substitution in immunoglobulin domain-1. - Auberger System Parsons et al. (1997) demonstrated that the Au(a)/Au(b) polymorphism resulted from a variation in the G strand of domain 5 of the BCAM gene (T539A; 612773.0002) ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 111200 was added.
Sept. 16, 2015: Protein entry updated
Automatic update: model status changed